Skip to content

TAD calling weird behaviour #392

Description

@iagooteroc

Hi. I'm doing TAD calling using Chromosome.find_tad on two samples that have 5kb resolution. At first I did the calling with add_experiment(..., resolution=5000), but that resulted in very small TADs. After that, I tried with resolution=40000 and the TADs look good (see figure below)
chr12

However, there are still two chromosomes that have very small TADs, chr13 for example:
chr13

I tried to re-run those chromosomes with 20k and 80k resolution to no avail, it still results in small TADs.
I'm running pytadbit v1.0.1 (pytadbit.version says v1.1 though) and this is my code:

from pytadbit.parsers.hic_parser import load_hic_data_from_bam
from pytadbit import Chromosome

hic_data = load_hic_data_from_bam(BAM_FILE, int(RESOLUTION), region=CHROMOSOME, ncpus=int(THREADS))
crm = Chromosome(CHROMOSOME)
crm.add_experiment('EXPERIMENT', hic_data=[hic_data.get_matrix(focus=CHROMOSOME)], resolution = int(RESOLUTION), ncpus = int(THREADS))
crm.find_tad([('EXPERIMENT')], n_cpus=int(THREADS), normalized=False) 
crm.get_experiment('EXPERIMENT').write_tad_borders(savedata='TAD_{0}_{1}.tsv'.format(CHROMOSOME, RESOLUTION))

Any insight would be appreciated, thank you.

Activity

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

      Milestone

      No milestone

      Relationships

      None yet

      Development

      No branches or pull requests

      Issue actions