not loading .ab1 input files

[vb-ak]

Hello!

I'm trying to load .ab1 input files in SOAPTyping but without success so far. The program loads and I am able to select the desired files as well as choose the appropriate HLA gene, exon number and F/R orientation, but when clicking on 'analysis' nothing happens. There is no error either.

Hoping you'll be able to point me in the right direction. I'm running under MacOS 11.6, and have tried on Amazon Linux 2 as well.

Thanks!

!

[jasonborn]

Thank you for your feedback. The file names that this software can load need to be named as follows: 18B0139284-A-8551-D01_A_2F_D01.ab1. By dividing ‘_’, the sample name, gene name, exon name, and gssp information can be obtained in turn.
So your test file name does not meet the loading requirements, you may need to adjust the naming and see if it works. Good luck !

[vb-ak]

Thank you! Files loaded successfully this way.

[vb-ak]

Hello again,
Another question on this: we did not use standard GSSPs but custom primers - do I need to load additional information on the primers for the .ab1 traces to be correctly interpreted? I see many mismatches to the top alleles (see attached screenshot), but I do not know if this is in the data, or if something is still not being loaded correctly.
Thank you so much!

[jasonborn]

This is normal and may be related to your data. This software is for personal use, and some situations may not be considered, and users need to modify it according to their needs. Good luck ！