From 90cfa3ca7476e8802038e1b9727fdf17eb762f00 Mon Sep 17 00:00:00 2001
From: Francesco <padovaf@tcd.ie>
Date: Tue, 27 Jan 2026 16:32:57 +0100
Subject: [PATCH 1/4] WIP: add a tool to annotate one mother-bud pair at the
 time

---
 cellacdc/docs/source/tooltips.rst | 12 ++++++++++++
 cellacdc/gui.py                   | 27 +++++++++++++++++++++++++--
 cellacdc/workers.py               |  2 +-
 3 files changed, 38 insertions(+), 3 deletions(-)

diff --git a/cellacdc/docs/source/tooltips.rst b/cellacdc/docs/source/tooltips.rst
index 0d545570..4cfb8be2 100644
--- a/cellacdc/docs/source/tooltips.rst
+++ b/cellacdc/docs/source/tooltips.rst
@@ -159,6 +159,12 @@
     :height: 16px
     :width: 16px
 
+.. |annotateSingleMotherBudPairButton| image:: https://raw.githubusercontent.com/SchmollerLab/Cell_ACDC/refs/heads/main/cellacdc/resources/icons/lock_id_annotate_future.svg
+    :target: https://github.com/SchmollerLab/Cell_ACDC/blob/main/cellacdc/resources/icons/lock_id_annotate_future.svg
+    :alt: annotateSingleMotherBudPairButton icon
+    :height: 16px
+    :width: 16px
+
 .. |segmentToolAction| image:: https://raw.githubusercontent.com/SchmollerLab/Cell_ACDC/refs/heads/main/cellacdc/resources/icons/segment.svg
     :target: https://github.com/SchmollerLab/Cell_ACDC/blob/main/cellacdc/resources/icons/segment.svg 
     :alt: segmentToolAction icon
@@ -478,6 +484,12 @@ Edit tools: Cell cycle analysis
 * **Automatically assign bud to mother (** |assignBudMothAutoAction| **):** Automatically assign buds to mothers using YeastMate.
 * **Manually edit cell cycle annotations table (** |editCcaToolAction| **"Ctrl+Shift+P"):** Manually edit cell cycle annotations table.
 * **Re-initialize cell cycle annotations table (** |reInitCcaAction| **):** Re-initialize cell cycle annotations table from this frame onward. NOTE: This will erase all the already annotated future frames information (from the current session not the saved information).
+* **Annotate one mother-bud pair at the time (** |annotateSingleMotherBudPairButton| **"Y"):**
+    1. Activate to annotate a single mother-bud pair at the time.
+    2. Annotate past and future frames
+    3. Deactivate to go back to the frame you were annotating before activating this tool.
+
+    NOTE: When annotating future frames, the other cells will not be displayed and they will be ignored. 
 
 Edit tools: Normal division: Lineage tree
 -----------------------------------------
diff --git a/cellacdc/gui.py b/cellacdc/gui.py
index 3bafb0ad..f6ab42f6 100755
--- a/cellacdc/gui.py
+++ b/cellacdc/gui.py
@@ -1010,6 +1010,25 @@ def gui_createToolBars(self):
         self.checkableQButtonsGroup.addButton(self.setIsHistoryKnownButton)
         self.functionsNotTested3D.append(self.setIsHistoryKnownButton)
         
+        self.annotateSingleMotherBudPairButton = QToolButton(self)
+        self.annotateSingleMotherBudPairButton.setIcon(
+            QIcon(":lock_id_annotate_future.svg")
+        )
+        self.annotateSingleMotherBudPairButton.setCheckable(True)
+        self.annotateSingleMotherBudPairButton.setShortcut('Y')
+        self.annotateSingleMotherBudPairButton.setVisible(False)
+        self.annotateSingleMotherBudPairButton.action = ccaToolBar.addWidget(
+            self.annotateSingleMotherBudPairButton
+        )
+        self.checkableButtons.append(self.annotateSingleMotherBudPairButton)
+        self.widgetsWithShortcut['Annotate one mother-bud pair at the time'] = (
+            self.annotateSingleMotherBudPairButton
+        )
+        self.checkableQButtonsGroup.addButton(
+            self.annotateSingleMotherBudPairButton
+        )
+        self.functionsNotTested3D.append(self.annotateSingleMotherBudPairButton)
+        
         ccaToolBar.addAction(self.assignBudMothAutoAction)
         ccaToolBar.addAction(self.editCcaToolAction)
         ccaToolBar.addAction(self.reInitCcaAction)
@@ -14562,8 +14581,12 @@ def keyPressEvent(self, ev):
             return
 
         if ev.key() == Qt.Key_Q and self.debug:
-            # posData = self.data[self.pos_i]
-            printl(self.ax1.highlightingRectItems)
+            worker, thread = self.autoSaveActiveWorkers[-1]
+            printl(len(worker.dataQ))
+            printl(
+                self.manualAnnotPastButton.isChecked(),
+                self.annotateSingleMotherBudPairButton.isChecked()
+            )
 
         if not self.isDataLoaded:
             self.logger.warning(
diff --git a/cellacdc/workers.py b/cellacdc/workers.py
index 04dbab37..6b3b7392 100755
--- a/cellacdc/workers.py
+++ b/cellacdc/workers.py
@@ -836,7 +836,7 @@ def __init__(self, mutex, waitCond, savedSegmData):
         self.abortSaving = False
         self.isSaving = False
         self.isPaused = False
-        self.dataQ = deque(maxlen=5)
+        self.dataQ = deque(maxlen=2)
         self.isAutoSaveON = False
         self.debug = False
     

From 9ad32511cd0162b08ed326beb425b9656afe307b Mon Sep 17 00:00:00 2001
From: Francesco <padovaf@tcd.ie>
Date: Tue, 27 Jan 2026 19:39:36 +0100
Subject: [PATCH 2/4] wip: setup annotate single mother bud tool

---
 cellacdc/_warnings.py |  28 +++++++++
 cellacdc/gui.py       | 142 ++++++++++++++++++++++++++++++++++++++++--
 2 files changed, 164 insertions(+), 6 deletions(-)

diff --git a/cellacdc/_warnings.py b/cellacdc/_warnings.py
index b50d1987..5645c9d8 100644
--- a/cellacdc/_warnings.py
+++ b/cellacdc/_warnings.py
@@ -202,6 +202,34 @@ def warnPromptSegmentPointsLayerNotInit(qparent=None):
     )
     return msg.cancel
 
+def warnNoIDsInS(qparent=None):
+    from cellacdc import widgets
+    txt = html_utils.paragraph(f"""
+        <b>None</b> of the IDs present at this frame 
+        <b>are in 'S' phase</b>.<br><br>
+        This tool can be used only for mother-bud pairs.<br><br>
+        Thank you for your patience!
+    """)
+    msg = widgets.myMessageBox(wrapText=False)
+    msg.warning(
+        qparent, 'No cells in "S" phase', txt, 
+    )
+    return msg.cancel
+
+def warnSelectedIDisNotInS(ID, qparent=None):
+    from cellacdc import widgets
+    txt = html_utils.paragraph(f"""
+        The selected ID {ID} <b>cell cycle stage is not 'S'</b>!<br><br>
+        Make sure you are hovering a cell ID in 'S' (mother of bud), 
+        when activating this mode.<br><br>
+        Thank you for your patience!
+    """)
+    msg = widgets.myMessageBox(wrapText=False)
+    msg.warning(
+        qparent, 'Selected ID not in S', txt, 
+    )
+    return msg.cancel
+
 def warnPromptSegmentModelNotInit(qparent=None):
     from cellacdc import widgets
     txt = html_utils.paragraph(f"""
diff --git a/cellacdc/gui.py b/cellacdc/gui.py
index f6ab42f6..81298187 100755
--- a/cellacdc/gui.py
+++ b/cellacdc/gui.py
@@ -3346,9 +3346,13 @@ def gui_connectEditActions(self):
 
         self.whitelistIDsToolbar.sigViewOGIDs.connect(self.whitelistViewOGIDs)
 
-        self.whitelistIDsToolbar.sigAddNewIDs.connect(self.whitelistAddNewIDsToggled)
+        self.whitelistIDsToolbar.sigAddNewIDs.connect(
+            self.whitelistAddNewIDsToggled
+        )
 
-        self.whitelistIDsToolbar.sigLoadOGLabs.connect(self.whitelistLoadOGLabs_cb)
+        self.whitelistIDsToolbar.sigLoadOGLabs.connect(
+            self.whitelistLoadOGLabs_cb
+        )
 
         self.whitelistIDsToolbar.sigTrackOGagainstPreviousFrame.connect(
             self.whitelistTrackOGagainstPreviousFrame_cb
@@ -3370,6 +3374,10 @@ def gui_connectEditActions(self):
         self.addScaleBarAction.toggled.connect(self.addScaleBar)
         self.addTimestampAction.toggled.connect(self.addTimestamp)
         self.saveLabColormapAction.triggered.connect(self.saveLabelsColormap)
+        
+        self.annotateSingleMotherBudPairButton.toggled.connect(
+            self.annotateSingleMotherBudPair_cb
+        )
 
         self.enableSmartTrackAction.toggled.connect(self.enableSmartTrack)
         # Brush/Eraser size action
@@ -3381,18 +3389,26 @@ def gui_connectEditActions(self):
         self.modeComboBox.activated.connect(self.clearComboBoxFocus)
         self.equalizeHistPushButton.toggled.connect(self.equalizeHist)
         
-        self.editOverlayColorAction.triggered.connect(self.toggleOverlayColorButton)
-        self.editTextIDsColorAction.triggered.connect(self.toggleTextIDsColorButton)
+        self.editOverlayColorAction.triggered.connect(
+            self.toggleOverlayColorButton
+        )
+        self.editTextIDsColorAction.triggered.connect(
+            self.toggleTextIDsColorButton
+        )
         self.overlayColorButton.sigColorChanging.connect(self.changeOverlayColor)
         self.overlayColorButton.sigColorChanged.connect(self.saveOverlayColor)
-        self.textIDsColorButton.sigColorChanging.connect(self.updateTextAnnotColor)
+        self.textIDsColorButton.sigColorChanging.connect(
+            self.updateTextAnnotColor
+        )
         self.textIDsColorButton.sigColorChanged.connect(self.saveTextIDsColors)
 
         self.setMeasurementsAction.triggered.connect(self.showSetMeasurements)
         self.addCustomMetricAction.triggered.connect(self.addCustomMetric)
         self.addCombineMetricAction.triggered.connect(self.addCombineMetric)
 
-        self.labelsGrad.colorButton.sigColorChanging.connect(self.updateBkgrColor)
+        self.labelsGrad.colorButton.sigColorChanging.connect(
+            self.updateBkgrColor
+        )
         self.labelsGrad.colorButton.sigColorChanged.connect(self.saveBkgrColor)
         self.labelsGrad.sigGradientChangeFinished.connect(self.updateLabelsCmap)
         self.labelsGrad.sigGradientChanged.connect(self.ticksCmapMoved)
@@ -13032,6 +13048,102 @@ def copyLostObjContour_cb(self, checked):
         self.lostObjImage = np.zeros_like(self.currentLab2D)
         self.updateLostContoursImage(0)
     
+    def getHoveredMotherBudIDs(self):
+        posData = self.data[self.pos_i]
+        
+        hoverID = self.getLastHoveredID()
+        try:
+            ccs = posData.cca_df.loc[hoverID, 'cell_cycle_stage']
+        except Exception as err:
+            ccs = 'G1'
+        
+        cca_df = posData.cca_df
+        cca_df_S = cca_df[cca_df['cell_cycle_stage'] == 'S']
+        IDs_in_S = cca_df_S.index.to_list()
+        
+        if not IDs_in_S:
+            _warnings.warnNoIDsInS(qparent=self)
+            return
+        
+        while ccs != 'S':
+            win = apps.QLineEditDialog(
+                title='Selected ID not in S',
+                msg='The cell cycle stage of the selected ID is not "S"\n'
+                    'Enter the ID (mother or bud) '
+                    'that you want to annotate.',
+                parent=self, 
+                isInteger=True,
+                allowedValues=IDs_in_S,
+                defaultTxt=IDs_in_S[0]
+            )
+            win.exec_()
+            if win.cancel:
+                return
+            
+            hoverID = win.EntryID
+            ccs = cca_df.loc[hoverID, 'cell_cycle_stage']
+        
+        relationship = posData.cca_df.loc[hoverID, 'relationship']
+        relative_ID = posData.cca_df.loc[hoverID, 'relative_ID']
+        mothID = hoverID if relationship == 'mother' else relative_ID
+        budID = hoverID if relationship == 'bud' else relative_ID
+        
+        return mothID, budID
+    
+    def annotateSingleMotherBudPair_cb(self, checked):
+        posData = self.data[self.pos_i]
+        self.modeComboBox.setDisabled(checked)
+        if checked:
+            self.store_data()
+            
+            self.annotateSingleMothBudPairState = {}
+            mothIDbudID = self.getHoveredMotherBudIDs()
+            
+            if mothIDbudID is None:
+                self.annotateSingleMotherBudPairButton.setChecked(False)
+                return
+            
+            mothID, budID = mothIDbudID
+            
+            self.logger.info(
+                'Setting annotation mode for single mother-bud pair = '
+                f'{(mothID, budID)}, at frame n. {posData.frame_i+1}'
+            )
+            
+            self.annotateSingleMothBudPairState['mother_ID'] = (
+                mothID
+            )
+            self.annotateSingleMothBudPairState['bud_ID'] = (
+                budID
+            )
+            self.annotateSingleMothBudPairState['frame_i_to_restore'] = (
+                posData.frame_i
+            )
+            
+            self.ax1.sigRangeChanged.connect(self.highlightManualAnnotMode)
+            self.ax1.setHighlighted(True, color='green')
+        else:
+            frame_to_restore = self.manualAnnotState.get('frame_i_to_restore')
+            if frame_to_restore is None:
+                return            
+            
+            self.store_data()
+            self.store_manual_annot_data()
+            
+            last_tracked_i_to_restore = self.manualAnnotState['last_tracked_i']
+            self.manualAnnotRestoreLastTrackedFrame(last_tracked_i_to_restore)
+            
+            self.logger.info(
+                f'Restoring view to frame n. {posData.frame_i+1}...'
+            )
+            posData.frame_i = frame_to_restore
+            self.get_data()
+            self.updateAllImages()
+            self.updateScrollbars()
+            self.ax1.sigRangeChanged.disconnect()
+            self.ax1.setHighlighted(False)
+            QTimer.singleShot(150, self.autoRange)
+
     def manualAnnotPast_cb(self, checked):
         posData = self.data[self.pos_i]
         if checked:
@@ -27994,6 +28106,24 @@ def setTitleText(
             self, lost_IDs=None, new_IDs=None, IDs_with_holes=None, 
             tracked_lost_IDs=None
         ):
+        if self.annotateSingleMotherBudPairButton.isChecked():
+            mothID = self.annotateSingleMothBudPairState.get(
+                'mother_ID'
+            )
+            budID = self.annotateSingleMothBudPairState.get(
+                'bud_ID'
+            )
+            frame_to_restore = self.annotateSingleMothBudPairState.get(
+                'frame_i_to_restore'
+            )
+            txt = (
+                f'Annotating mother-bud pair {(mothID, budID)} '
+                f'since frame n. {frame_to_restore+1}'
+            )
+            htmlTxt = f'<font color="orange">{txt}</font>'
+            self.titleLabel.setText(htmlTxt)
+            return
+        
         if self.manualAnnotPastButton.isChecked():
             lockedID = self.editIDspinbox.value()
             frame_to_restore = self.manualAnnotState.get('frame_i_to_restore')

From 88bea46ce9e82de01c312b4e3d2ec4eca9d30aa8 Mon Sep 17 00:00:00 2001
From: Francesco <padovaf@tcd.ie>
Date: Wed, 28 Jan 2026 17:27:36 +0100
Subject: [PATCH 3/4] feat: annotate single mother-bud pair tool, closes #985

---
 cellacdc/cca_functions.py                   |  34 +++
 cellacdc/gui.py                             | 245 ++++++++++++++++----
 cellacdc/load.py                            |  35 ++-
 cellacdc/myutils.py                         |   3 +-
 global_cca_df_with_single_moth_bud_pair.csv | 124 ++++++++++
 5 files changed, 380 insertions(+), 61 deletions(-)
 create mode 100644 global_cca_df_with_single_moth_bud_pair.csv

diff --git a/cellacdc/cca_functions.py b/cellacdc/cca_functions.py
index cd87389b..2ca15a5a 100755
--- a/cellacdc/cca_functions.py
+++ b/cellacdc/cca_functions.py
@@ -864,7 +864,41 @@ def add_generation_num_of_relative_ID(
     
     acdc_df_with_col = acdc_df_by_frame_i.reset_index()
     return acdc_df_with_col
+
+def get_IDs_gen_num_will_divide(cca_df):
+    """Get a list of (ID, gen_num) of cells that require `will_divide`>0
+    
+    Parameters
+    ----------
+    cca_df : pd.DataFrame
+        DataFrame with cc annotations for every frame and Cell_ID
     
+    Returns
+    -------
+    list of tuples
+        List of (ID, gen_num) of cells that require `will_divide`>0
+    """
+    
+    cca_df_buds = cca_df.query('relationship == "bud"')
+    IDs_gen_num_will_divide = []
+    for budID, bud_cca_df in cca_df_buds.groupby('Cell_ID'):
+        all_gen_nums = cca_df.query(f'Cell_ID == {budID}')['generation_num']
+        if not (all_gen_nums > 0).any():
+            # bud division is annotated in the future
+            continue        
+        
+        mothID = int(bud_cca_df['relative_ID'].iloc[0])
+        first_frame_bud = bud_cca_df['frame_i'].iloc[0]
+        gen_num_moth = cca_df.query(
+            f'(frame_i == {first_frame_bud}) & (Cell_ID == {mothID})'
+        )['generation_num'].iloc[0]
+        
+        IDs_gen_num_will_divide.append((mothID, gen_num_moth))
+        IDs_gen_num_will_divide.append((budID, 0))
+        
+    return IDs_gen_num_will_divide
+    
+
 def get_IDs_gen_num_will_divide_wrong(global_cca_df):
     """Get a list of (ID, gen_num) of cells whose `will_divide`>0 but the 
     next generation does not exist (i.e., `will_divide` is wrong)
diff --git a/cellacdc/gui.py b/cellacdc/gui.py
index 81298187..6709272b 100755
--- a/cellacdc/gui.py
+++ b/cellacdc/gui.py
@@ -1568,6 +1568,7 @@ def autoSaveWorkerTimerCallback(self, worker, posData):
             worker._enqueue(posData)
     
     def autoSaveWorkerDone(self):
+        self.logger.info('Autosaving done.')
         self.setStatusBarLabel(log=False)
     
     def ccaCheckerWorkerDone(self):
@@ -13068,7 +13069,8 @@ def getHoveredMotherBudIDs(self):
         while ccs != 'S':
             win = apps.QLineEditDialog(
                 title='Selected ID not in S',
-                msg='The cell cycle stage of the selected ID is not "S"\n'
+                msg='The <b>cell cycle stage</b> of the selected ID '
+                    '<b>is not "S"</b>.<br><br>'
                     'Enter the ID (mother or bud) '
                     'that you want to annotate.',
                 parent=self, 
@@ -13088,7 +13090,7 @@ def getHoveredMotherBudIDs(self):
         mothID = hoverID if relationship == 'mother' else relative_ID
         budID = hoverID if relationship == 'bud' else relative_ID
         
-        return mothID, budID
+        return int(mothID), int(budID)
     
     def annotateSingleMotherBudPair_cb(self, checked):
         posData = self.data[self.pos_i]
@@ -13110,6 +13112,13 @@ def annotateSingleMotherBudPair_cb(self, checked):
                 f'{(mothID, budID)}, at frame n. {posData.frame_i+1}'
             )
             
+            self.annotateSingleMothBudPairState['doWarnLostObj'] = (
+                self.warnLostCellsAction.isChecked()
+            )
+            self.annotateSingleMothBudPairState['doAnnotateLostObjs'] = (
+                self.annotLostObjsToggle.isChecked()
+            )
+            
             self.annotateSingleMothBudPairState['mother_ID'] = (
                 mothID
             )
@@ -13119,29 +13128,49 @@ def annotateSingleMotherBudPair_cb(self, checked):
             self.annotateSingleMothBudPairState['frame_i_to_restore'] = (
                 posData.frame_i
             )
+            self.annotateSingleMothBudPairState['last_cca_frame_i'] = (
+                self.navigateScrollBar.maximum()-1
+            )
+            
+            self.warnLostCellsAction.setChecked(False)
+            self.annotLostObjsToggle.setChecked(False)
             
             self.ax1.sigRangeChanged.connect(self.highlightManualAnnotMode)
             self.ax1.setHighlighted(True, color='green')
         else:
-            frame_to_restore = self.manualAnnotState.get('frame_i_to_restore')
+            frame_to_restore = self.annotateSingleMothBudPairState.get(
+                'frame_i_to_restore'
+            )
             if frame_to_restore is None:
                 return            
             
-            self.store_data()
-            self.store_manual_annot_data()
-            
-            last_tracked_i_to_restore = self.manualAnnotState['last_tracked_i']
-            self.manualAnnotRestoreLastTrackedFrame(last_tracked_i_to_restore)
+            self.store_single_mother_bud_pair_data()
             
             self.logger.info(
                 f'Restoring view to frame n. {posData.frame_i+1}...'
             )
             posData.frame_i = frame_to_restore
+            last_cca_frame_i_to_restore = (
+                self.annotateSingleMothBudPairState['last_cca_frame_i']
+            )
+            self.annotateSingleMotherBudPairRestoreLastCcaFrame(
+                last_cca_frame_i_to_restore
+            )
             self.get_data()
             self.updateAllImages()
             self.updateScrollbars()
             self.ax1.sigRangeChanged.disconnect()
             self.ax1.setHighlighted(False)
+            
+            self.warnLostCellsAction.setChecked(
+                self.annotateSingleMothBudPairState['doWarnLostObj']
+            )
+            self.annotLostObjsToggle.setChecked(
+                self.annotateSingleMothBudPairState['doAnnotateLostObjs']
+            )
+            
+            self.annotateSingleMothBudPairState = {}
+            
             QTimer.singleShot(150, self.autoRange)
 
     def manualAnnotPast_cb(self, checked):
@@ -13238,18 +13267,32 @@ def highlightManualAnnotMode(self, viewBox, viewRange):
         self.ax1.setHighlighted(True)
     
     def updateHighlightedAxis(self):
-        if not self.manualAnnotPastButton.isChecked():
-            return
+        color = None
+        if self.manualAnnotPastButton.isChecked():
+            frame_to_restore = self.manualAnnotState.get('frame_i_to_restore')
+            posData = self.data[self.pos_i]
+            if posData.frame_i == frame_to_restore:
+                color = 'green'
+            elif posData.frame_i < frame_to_restore:
+                color = 'gold'
+            else:
+                color = 'red'
         
-        frame_to_restore = self.manualAnnotState.get('frame_i_to_restore')
-        posData = self.data[self.pos_i]
-        if posData.frame_i == frame_to_restore:
-            color = 'green'
-        elif posData.frame_i < frame_to_restore:
-            color = 'gold'
-        else:
-            color = 'red'
+        if self.annotateSingleMotherBudPairButton.isChecked():
+            frame_to_restore = self.annotateSingleMothBudPairState.get(
+                'frame_i_to_restore'
+            )
+            posData = self.data[self.pos_i]
+            if posData.frame_i == frame_to_restore:
+                color = 'green'
+            elif posData.frame_i < frame_to_restore:
+                color = 'gold'
+            else:
+                color = 'red'
         
+        if color is None:
+            return 
+                
         self.ax1.setHighlightingRectItemsColor(color)
         
     def updateLostNewCurrentIDs(self):
@@ -14693,12 +14736,8 @@ def keyPressEvent(self, ev):
             return
 
         if ev.key() == Qt.Key_Q and self.debug:
-            worker, thread = self.autoSaveActiveWorkers[-1]
-            printl(len(worker.dataQ))
-            printl(
-                self.manualAnnotPastButton.isChecked(),
-                self.annotateSingleMotherBudPairButton.isChecked()
-            )
+            posData = self.data[self.pos_i]
+            printl(posData.new_IDs)
 
         if not self.isDataLoaded:
             self.logger.warning(
@@ -18632,8 +18671,16 @@ def manualAnnotRestoreLastTrackedFrame(self, last_tracked_i_to_restore):
             
             posData.allData_li[frame_i] = data_frame_i
         
-        self.navigateScrollBar.setMaximum(last_tracked_i_to_restore+1)
-        self.navSpinBox.setMaximum(last_tracked_i_to_restore+1)
+        posData.last_tracked_i = last_tracked_i_to_restore
+        self.setNavigateScrollBarMaximum()
+    
+    def annotateSingleMotherBudPairRestoreLastCcaFrame(
+            self, cca_frame_i_to_restore
+        ):
+        if self.navigateScrollBar.maximum()-1 <= cca_frame_i_to_restore:
+            return
+        
+        self.resetCcaFuture(cca_frame_i_to_restore+1)
     
     def setNavigateScrollBarMaximum(self):
         posData = self.data[self.pos_i]
@@ -20509,6 +20556,8 @@ def initGlobalAttr(self):
         self.timestampDialog = None
         self.scaleBarDialog = None
         self.countObjsWindow = None
+        
+        self.annotateSingleMothBudPairState = {}
 
         # Second channel used by cellpose
         self.secondChannelName = None
@@ -20889,6 +20938,40 @@ def store_manual_annot_data(
             
         # data_frame_i['manually_edited_lab']['zoom_slice'] = zoom_slice
 
+    def store_single_mother_bud_pair_data(self, cca_df=None):
+        posData = self.data[self.pos_i]
+        data_frame_i = posData.allData_li[posData.frame_i]
+        
+        if cca_df is None:
+            cca_df = data_frame_i['acdc_df']
+        
+        if cca_df is None:
+            return
+        
+        mothID = self.annotateSingleMothBudPairState.get('mother_ID')
+        if mothID is None:
+            return        
+        
+        budID = self.annotateSingleMothBudPairState['bud_ID']
+        single_moth_bud_pair_cca = cca_df.loc[[mothID, budID]].copy()
+        stored_moth_bud_pairs_cca = data_frame_i.get('moth_bud_pairs_cca')
+        if stored_moth_bud_pairs_cca is None:
+            data_frame_i['moth_bud_pairs_cca'] = single_moth_bud_pair_cca
+        else:
+            if mothID in stored_moth_bud_pairs_cca.index:
+                stored_moth_bud_pairs_cca = stored_moth_bud_pairs_cca.drop(
+                    index=mothID
+                )
+            
+            if budID in stored_moth_bud_pairs_cca.index:
+                stored_moth_bud_pairs_cca = stored_moth_bud_pairs_cca.drop(
+                    index=budID
+                )
+                
+            data_frame_i['moth_bud_pairs_cca'] = pd.concat(
+                [stored_moth_bud_pairs_cca, single_moth_bud_pair_cca],
+            ).drop_duplicates()
+    
     @exception_handler
     def store_data(
             self, pos_i=None, enforce=True, debug=False, mainThread=True,
@@ -20985,6 +21068,7 @@ def store_data(
 
         if not mainThread:
             self.pointsLayerDataToDf(posData)
+            
         self.store_cca_df(
             pos_i=pos_i, mainThread=mainThread, autosave=autosave, 
             store_cca_df_copy=store_cca_df_copy
@@ -21216,7 +21300,7 @@ def checkCcaPastFramesNewIDs(self):
             
             # Remove IDs found in past frames from new_IDs list
             newIDs = np.array(posData.new_IDs, dtype=np.uint32)
-            mask_index = np.in1d(newIDs, cca_df_i.index)
+            mask_index = np.isin(newIDs, cca_df_i.index)
             posData.new_IDs = list(newIDs[~mask_index])
             if not posData.new_IDs:
                 return found_cca_df_IDs
@@ -21350,6 +21434,28 @@ def _getCcaCostMatrix(
         
         return cost
 
+    def getSingleMotherBudPairCca_df(self):
+        if not self.annotateSingleMotherBudPairButton.isChecked():
+            return
+        
+        posData = self.data[self.pos_i]
+        start_frame_i = (
+            self.annotateSingleMothBudPairState['frame_i_to_restore']
+        )
+        
+        if posData.frame_i <= start_frame_i:
+            # Past frame --> no need to get cca_df
+            return
+        
+        mothID = self.annotateSingleMothBudPairState['mother_ID']
+        budID = self.annotateSingleMothBudPairState['bud_ID']
+        
+        # Get previous dataframe
+        acdc_df = posData.allData_li[posData.frame_i-1]['acdc_df']
+        prev_cca_df = acdc_df[self.cca_df_colnames].loc[[mothID, budID]].copy()
+        
+        return prev_cca_df
+    
     def autoCca_df(self, enforceAll=False):
         """
         Assign each bud to a mother with scipy linear sum assignment
@@ -21375,6 +21481,13 @@ def autoCca_df(self, enforceAll=False):
             proceed = self.warnFrameNeverVisitedSegmMode()
             return notEnoughG1Cells, proceed
         
+        # Get temporary cca_df with single mother-bud pair mode active
+        cca_df = self.getSingleMotherBudPairCca_df()
+        if cca_df is not None:
+            posData.cca_df = cca_df
+            self.store_single_mother_bud_pair_data(cca_df=cca_df)
+            return notEnoughG1Cells, proceed
+        
         # Determine if this is the last visited frame for repeating
         # bud assignment on non manually correct (corrected_on_frame_i>0) buds.
         # The idea is that the user could have assigned division on a cell
@@ -21385,15 +21498,33 @@ def autoCca_df(self, enforceAll=False):
             curr_df, enforceAll=enforceAll
         )
         
-        frameAlreadyAnnotated = (
+        # Get previous dataframe
+        acdc_df = posData.allData_li[posData.frame_i-1]['acdc_df']
+        prev_cca_df = acdc_df[self.cca_df_colnames].copy()
+        
+        # When using "single mother-bud pair annotation" mode, 
+        # there might be some IDs that do not have annotations yet
+        # --> not fully annotated frame
+        missingIDs = []
+        if posData.cca_df is not None:
+            missingIDs = [
+                ID for ID in posData.IDs 
+                if ID not in posData.cca_df.index
+                and ID in prev_cca_df.index
+            ]
+        
+        frameAlreadyFullyAnnotated = (
             posData.cca_df is not None
             and not enforceAll
             and not isLastVisitedAgain
+            and not missingIDs
         )
         # Use stored cca_df and do not modify it with automatic stuff
-        if frameAlreadyAnnotated:
+        if frameAlreadyFullyAnnotated:
             return notEnoughG1Cells, proceed
         
+        self.updateLostNewCurrentIDs()
+        
         # Keep only correctedAssignIDs if requested
         # For the last visited frame we perform assignment again only on
         # IDs where we didn't manually correct assignment
@@ -21419,22 +21550,24 @@ def autoCca_df(self, enforceAll=False):
             notEnoughG1Cells = False
             proceed = False
             return notEnoughG1Cells, proceed
-
-        # Get previous dataframe
-        acdc_df = posData.allData_li[posData.frame_i-1]['acdc_df']
-        prev_cca_df = acdc_df[self.cca_df_colnames].copy()
-
+        
         if posData.cca_df is None:
             posData.cca_df = prev_cca_df.copy()
         else:
             posData.cca_df = curr_df[self.cca_df_colnames].copy()
         
+        if missingIDs:
+            # Add missing IDs from previous cca_df
+            posData.cca_df.loc[missingIDs] = prev_cca_df.loc[missingIDs]
+        
+        posData.cca_df = posData.cca_df.dropna()
+        
         # concatenate new IDs found in past frames (before frame_i-1)
         if found_cca_df_IDs is not None:
             cca_df = pd.concat([posData.cca_df, *found_cca_df_IDs])
             unique_idx = ~cca_df.index.duplicated(keep='first')
             posData.cca_df = cca_df[unique_idx]
-
+        
         # If there are no new IDs we are done
         if not posData.new_IDs:
             proceed = True
@@ -21744,7 +21877,7 @@ def set_2Dlab(self, lab2D):
         else:
             posData.lab = lab2D
 
-    def get_labels(
+    def get_labels_array(
             self, 
             from_store=False, 
             frame_i=None, 
@@ -21825,7 +21958,7 @@ def _get_editID_info(self, df):
             for row in manually_edited_df.itertuples()
         ]
         return editID_info
-
+    
     def apply_manual_edits_to_lab_if_needed(self, lab):
         posData = self.data[self.pos_i]
         data_frame_i = posData.allData_li[posData.frame_i]
@@ -21869,7 +22002,7 @@ def _get_data_unvisited(self, posData, debug=False, lin_tree_init=True,):
 
         elif str(self.modeComboBox.currentText()) == 'Normal division: Lineage tree':
             # Warn that we are visiting a frame that was never segm-checked
-            # on cell cycle analysis mode
+            # on Normal division: Lineage tree mode
             msg = widgets.myMessageBox()
             txt = html_utils.paragraph(
                 'Segmentation and Tracking was <b>never checked from '
@@ -21885,10 +22018,8 @@ def _get_data_unvisited(self, posData, debug=False, lin_tree_init=True,):
             return proceed_cca, never_visited
         
         # Requested frame was never visited before. Load from HDD
-        labels = self.get_labels()
-        posData.lab = self.apply_manual_edits_to_lab_if_needed(
-            labels
-        )
+        lab = self.get_labels_array()
+        posData.lab = self.apply_manual_edits_to_lab_if_needed(lab)
         posData.rp = skimage.measure.regionprops(posData.lab)
         self.setManualBackgroundLab()
         
@@ -21933,7 +22064,7 @@ def _get_data_unvisited(self, posData, debug=False, lin_tree_init=True,):
     def _get_data_visited(self, posData, debug=False, lin_tree_init=True,):        
         # Requested frame was already visited. Load from RAM.
         never_visited = False
-        posData.lab = self.get_labels(from_store=True)
+        posData.lab = self.get_labels_array(from_store=True)
         posData.rp = skimage.measure.regionprops(posData.lab)
         df = posData.allData_li[posData.frame_i]['acdc_df']
         if df is None:
@@ -22144,8 +22275,8 @@ def initCca(self):
                     'No, stay on current frame')
             )
             if goToFrameButton == msg.clickedButton:
-                self.addMissingIDs_cca_df(posData)
-                self.store_cca_df()
+                # self.addMissingIDs_cca_df(posData)
+                # self.store_cca_df()
                 msg = 'Looking good!'
                 self.last_cca_frame_i = last_cca_frame_i
                 posData.frame_i = last_cca_frame_i
@@ -22415,7 +22546,9 @@ def resetWillDivideInfo(self):
         if global_cca_df is None:
             return
         
+        global_cca_df.to_csv('global_cca_df_with_single_moth_bud_pair.csv')
         global_cca_df = load._fix_will_divide(global_cca_df)
+        
         self.storeFromConcatCcaDf(global_cca_df)
     
     def ccaCheckerStopChecking(self):
@@ -22534,9 +22667,8 @@ def get_cca_df(self, frame_i=None, return_df=False, debug=False):
         cca_df = None
         i = posData.frame_i if frame_i is None else frame_i
         df = posData.allData_li[i]['acdc_df']            
-        if df is not None:
-            if 'cell_cycle_stage' in df.columns:
-                cca_df = df[self.cca_df_colnames].copy()
+        if df is not None and 'cell_cycle_stage' in df.columns:
+            cca_df = df[self.cca_df_colnames].copy()
         
         if cca_df is None and self.isSnapshot:
             cca_df = self.getBaseCca_df()
@@ -22544,6 +22676,12 @@ def get_cca_df(self, frame_i=None, return_df=False, debug=False):
 
         if cca_df is not None:
             cca_df = cca_df.dropna()
+        else:
+            moth_bud_pairs_cca = (
+                posData.allData_li[i].get('moth_bud_pairs_cca', None)
+            )
+            if moth_bud_pairs_cca is not None:
+                cca_df = moth_bud_pairs_cca
         
         if return_df:
             return cca_df
@@ -22666,11 +22804,16 @@ def store_cca_df(
         if store_cca_df_copy and cca_df is not None:
             posData.allData_li[i]['cca_df'] = cca_df.copy()
         
+        if self.annotateSingleMotherBudPairButton.isChecked():
+            self.store_single_mother_bud_pair_data(cca_df=cca_df)
+        
         if autosave:
             self.enqAutosave()
             self.enqCcaIntegrityChecker()
 
-    def lin_tree_to_acdc_df(self, force_all=False, ignore=set(), force=set(), specific=set()):
+    def lin_tree_to_acdc_df(
+            self, force_all=False, ignore=set(), force=set(), specific=set()
+        ):
         """
         Syncs the lineage tree DataFrame with the acdc_df DataFrame. By default, it will only try to sync frames which have not been synced before. 
         This can be changed using the optional arguments.
@@ -28050,7 +28193,7 @@ def getPrevFrameIDs(self, current_frame_i=None):
             return prevIDs
         
         # IDs in previous frame were not stored --> load prev lab from HDD
-        prev_lab = self.get_labels(
+        prev_lab = self.get_labels_array(
             from_store=False, 
             frame_i=prev_frame_i,
             return_copy=False
@@ -28660,7 +28803,7 @@ def getTrackedLostIDs(self, prev_lab=None, IDs_in_frames=None, frame_i=None):
             frame_i = posData.frame_i
             
         if prev_lab is None:
-            prev_lab = self.get_labels(
+            prev_lab = self.get_labels_array(
                 from_store=True, 
                 frame_i=posData.frame_i-1, 
                 return_existing=False,
diff --git a/cellacdc/load.py b/cellacdc/load.py
index 051a6608..f107e118 100755
--- a/cellacdc/load.py
+++ b/cellacdc/load.py
@@ -517,8 +517,7 @@ def _fix_corrected_assignment_i(acdc_df: pd.DataFrame):
 
 def _fix_will_divide(acdc_df):
     """Resetting annotaions in GUI sometimes does not fully reset `will_divide` 
-    column. Here we set `will_divide` back to 0 for those cells whose 
-    next generation does not exist (division was not annotated)
+    column. Here we reset `will_divide`
 
     Parameters
     ----------
@@ -534,19 +533,22 @@ def _fix_will_divide(acdc_df):
     if 'cell_cycle_stage' not in acdc_df.columns:
         return acdc_df
     
-    required_cols = ['frame_i', 'Cell_ID', 'generation_num', 'will_divide']
+    required_cols = [
+        'frame_i', 'Cell_ID', 'generation_num', 'will_divide', 'relationship',
+        'relative_ID'
+    ]
     
     cca_df_mask = ~acdc_df['cell_cycle_stage'].isna()
     cca_df = acdc_df[cca_df_mask].reset_index()[required_cols]
     
-    IDs_will_divide_wrong = (
-        cca_functions.get_IDs_gen_num_will_divide_wrong(cca_df)
-    )
-    if not IDs_will_divide_wrong:
+    IDs_gen_num_will_divide = cca_functions.get_IDs_gen_num_will_divide(cca_df)
+    
+    if not IDs_gen_num_will_divide:
         return acdc_df
     
-    cca_df = cca_df.reset_index().set_index(['Cell_ID', 'generation_num'])   
-    cca_df.loc[IDs_will_divide_wrong, 'will_divide'] = 0
+    cca_df['will_divide'] = 0.0
+    cca_df = cca_df.reset_index().set_index(['Cell_ID', 'generation_num'])
+    cca_df.loc[IDs_gen_num_will_divide, 'will_divide'] = 1.0
     cca_df = cca_df.reset_index()
     acdc_df = acdc_df.reset_index()
 
@@ -556,6 +558,21 @@ def _fix_will_divide(acdc_df):
     cca_df_index = cca_df_mask[cca_df_mask].index
     acdc_df.loc[cca_df_index, 'will_divide'] = cca_df['will_divide']
     
+    # IDs_will_divide_wrong = (
+    #     cca_functions.get_IDs_gen_num_will_divide_wrong(cca_df)
+    # )
+    # if IDs_will_divide_wrong:
+    #     cca_df = cca_df.reset_index().set_index(['Cell_ID', 'generation_num'])   
+    #     cca_df.loc[IDs_will_divide_wrong, 'will_divide'] = 0
+    #     cca_df = cca_df.reset_index()
+    #     acdc_df = acdc_df.reset_index()
+
+    #     cca_df = cca_df.set_index(['frame_i', 'Cell_ID'])
+    #     acdc_df = acdc_df.set_index(['frame_i', 'Cell_ID'])
+        
+    #     cca_df_index = cca_df_mask[cca_df_mask].index
+    #     acdc_df.loc[cca_df_index, 'will_divide'] = cca_df['will_divide']
+    
     return acdc_df
 
 def _add_missing_columns(acdc_df):
diff --git a/cellacdc/myutils.py b/cellacdc/myutils.py
index 8fad6fdd..5806ea69 100644
--- a/cellacdc/myutils.py
+++ b/cellacdc/myutils.py
@@ -5001,7 +5001,8 @@ def get_empty_stored_data_dict():
                     'rois': [], 'delMasks': [], 'delIDsROI': [], 'state': []
                 },
             'IDs': [],
-            'manually_edited_lab': {'lab': {}, 'zoom_slice': None}
+            'manually_edited_lab': {'lab': {}, 'zoom_slice': None},
+            'single_moth_bud_pair_cca': None,
         }
 
 def iterate_along_axes(arr, axes, arr_ndim=None):
diff --git a/global_cca_df_with_single_moth_bud_pair.csv b/global_cca_df_with_single_moth_bud_pair.csv
new file mode 100644
index 00000000..fe52ab37
--- /dev/null
+++ b/global_cca_df_with_single_moth_bud_pair.csv
@@ -0,0 +1,124 @@
+frame_i,Cell_ID,cell_cycle_stage,generation_num,relative_ID,relationship,emerg_frame_i,division_frame_i,is_history_known,corrected_on_frame_i,will_divide,daughter_disappears_before_division,disappears_before_division,time_minutes,time_hours,generation_num_tree,parent_ID_tree,root_ID_tree,sister_ID_tree,is_cell_dead,is_cell_excluded,was_manually_edited,x_centroid,y_centroid,num_objects,end_of_cell_cycle_frame_i,cell_area_pxl,cell_vol_vox,cell_area_um2,cell_vol_fl,basename,Dia_Ph3_autoBkgr_bkgrVal_median,Dia_Ph3_autoBkgr_bkgrVal_mean,Dia_Ph3_autoBkgr_bkgrVal_q75,Dia_Ph3_autoBkgr_bkgrVal_q25,Dia_Ph3_autoBkgr_bkgrVal_q95,Dia_Ph3_autoBkgr_bkgrVal_q05,Dia_Ph3_dataPrepBkgr_bkgrVal_median,Dia_Ph3_dataPrepBkgr_bkgrVal_mean,Dia_Ph3_dataPrepBkgr_bkgrVal_q75,Dia_Ph3_dataPrepBkgr_bkgrVal_q25,Dia_Ph3_dataPrepBkgr_bkgrVal_q95,Dia_Ph3_dataPrepBkgr_bkgrVal_q05,Dia_Ph3_mean,Dia_Ph3_sum,Dia_Ph3_amount_autoBkgr,Dia_Ph3_amount_dataPrepBkgr,Dia_Ph3_amount_manualBkgr,Dia_Ph3_mean_manualBkgr,Dia_Ph3_median,Dia_Ph3_min,Dia_Ph3_max,Dia_Ph3_q25,Dia_Ph3_q75,Dia_Ph3_q05,Dia_Ph3_q95,Dia_Ph3_concentration_autoBkgr_from_vol_vox,Dia_Ph3_concentration_dataPrepBkgr_from_vol_vox,Dia_Ph3_concentration_autoBkgr_from_vol_fl,Dia_Ph3_concentration_dataPrepBkgr_from_vol_fl,major_axis_length,minor_axis_length,inertia_tensor_eigvals-0,inertia_tensor_eigvals-1,equivalent_diameter,moments-0-0,moments-0-1,moments-0-2,moments-0-3,moments-1-0,moments-1-1,moments-1-2,moments-1-3,moments-2-0,moments-2-1,moments-2-2,moments-2-3,moments-3-0,moments-3-1,moments-3-2,moments-3-3,area,solidity,extent,inertia_tensor-0-0,inertia_tensor-0-1,inertia_tensor-1-0,inertia_tensor-1-1,filled_area,centroid-0,centroid-1,bbox_area,local_centroid-0,local_centroid-1,convex_area,euler_number,moments_normalized-0-0,moments_normalized-0-1,moments_normalized-0-2,moments_normalized-0-3,moments_normalized-1-0,moments_normalized-1-1,moments_normalized-1-2,moments_normalized-1-3,moments_normalized-2-0,moments_normalized-2-1,moments_normalized-2-2,moments_normalized-2-3,moments_normalized-3-0,moments_normalized-3-1,moments_normalized-3-2,moments_normalized-3-3,moments_central-0-0,moments_central-0-1,moments_central-0-2,moments_central-0-3,moments_central-1-0,moments_central-1-1,moments_central-1-2,moments_central-1-3,moments_central-2-0,moments_central-2-1,moments_central-2-2,moments_central-2-3,moments_central-3-0,moments_central-3-1,moments_central-3-2,moments_central-3-3,bbox-0,bbox-1,bbox-2,bbox-3,Dia_Ph3_CV,test_channel_indipendent_metric,velocity_pixel,velocity_um,disappears_before_end
+0,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+0,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+1,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+1,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+2,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+2,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+3,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+3,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+4,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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+49,7,S,0.0,2.0,bud,44.0,-1.0,True,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+50,2,S,3.0,7.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+50,7,S,0.0,2.0,bud,44.0,-1.0,True,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+51,2,S,3.0,7.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+51,7,S,0.0,2.0,bud,44.0,-1.0,True,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+52,2,S,3.0,7.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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+54,7,S,0.0,2.0,bud,44.0,-1.0,True,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+55,2,S,3.0,7.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+55,7,S,0.0,2.0,bud,44.0,-1.0,True,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+56,2,S,3.0,7.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+56,7,S,0.0,2.0,bud,44.0,-1.0,True,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
+57,2,S,3.0,7.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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From 1a2458ba8d3e4e05783e0ceef9a81597c5c8d973 Mon Sep 17 00:00:00 2001
From: Francesco <padovaf@tcd.ie>
Date: Thu, 29 Jan 2026 09:45:30 +0100
Subject: [PATCH 4/4] fix: delete unnecessary csv test file

---
 global_cca_df_with_single_moth_bud_pair.csv | 124 --------------------
 1 file changed, 124 deletions(-)
 delete mode 100644 global_cca_df_with_single_moth_bud_pair.csv

diff --git a/global_cca_df_with_single_moth_bud_pair.csv b/global_cca_df_with_single_moth_bud_pair.csv
deleted file mode 100644
index fe52ab37..00000000
--- a/global_cca_df_with_single_moth_bud_pair.csv
+++ /dev/null
@@ -1,124 +0,0 @@
-frame_i,Cell_ID,cell_cycle_stage,generation_num,relative_ID,relationship,emerg_frame_i,division_frame_i,is_history_known,corrected_on_frame_i,will_divide,daughter_disappears_before_division,disappears_before_division,time_minutes,time_hours,generation_num_tree,parent_ID_tree,root_ID_tree,sister_ID_tree,is_cell_dead,is_cell_excluded,was_manually_edited,x_centroid,y_centroid,num_objects,end_of_cell_cycle_frame_i,cell_area_pxl,cell_vol_vox,cell_area_um2,cell_vol_fl,basename,Dia_Ph3_autoBkgr_bkgrVal_median,Dia_Ph3_autoBkgr_bkgrVal_mean,Dia_Ph3_autoBkgr_bkgrVal_q75,Dia_Ph3_autoBkgr_bkgrVal_q25,Dia_Ph3_autoBkgr_bkgrVal_q95,Dia_Ph3_autoBkgr_bkgrVal_q05,Dia_Ph3_dataPrepBkgr_bkgrVal_median,Dia_Ph3_dataPrepBkgr_bkgrVal_mean,Dia_Ph3_dataPrepBkgr_bkgrVal_q75,Dia_Ph3_dataPrepBkgr_bkgrVal_q25,Dia_Ph3_dataPrepBkgr_bkgrVal_q95,Dia_Ph3_dataPrepBkgr_bkgrVal_q05,Dia_Ph3_mean,Dia_Ph3_sum,Dia_Ph3_amount_autoBkgr,Dia_Ph3_amount_dataPrepBkgr,Dia_Ph3_amount_manualBkgr,Dia_Ph3_mean_manualBkgr,Dia_Ph3_median,Dia_Ph3_min,Dia_Ph3_max,Dia_Ph3_q25,Dia_Ph3_q75,Dia_Ph3_q05,Dia_Ph3_q95,Dia_Ph3_concentration_autoBkgr_from_vol_vox,Dia_Ph3_concentration_dataPrepBkgr_from_vol_vox,Dia_Ph3_concentration_autoBkgr_from_vol_fl,Dia_Ph3_concentration_dataPrepBkgr_from_vol_fl,major_axis_length,minor_axis_length,inertia_tensor_eigvals-0,inertia_tensor_eigvals-1,equivalent_diameter,moments-0-0,moments-0-1,moments-0-2,moments-0-3,moments-1-0,moments-1-1,moments-1-2,moments-1-3,moments-2-0,moments-2-1,moments-2-2,moments-2-3,moments-3-0,moments-3-1,moments-3-2,moments-3-3,area,solidity,extent,inertia_tensor-0-0,inertia_tensor-0-1,inertia_tensor-1-0,inertia_tensor-1-1,filled_area,centroid-0,centroid-1,bbox_area,local_centroid-0,local_centroid-1,convex_area,euler_number,moments_normalized-0-0,moments_normalized-0-1,moments_normalized-0-2,moments_normalized-0-3,moments_normalized-1-0,moments_normalized-1-1,moments_normalized-1-2,moments_normalized-1-3,moments_normalized-2-0,moments_normalized-2-1,moments_normalized-2-2,moments_normalized-2-3,moments_normalized-3-0,moments_normalized-3-1,moments_normalized-3-2,moments_normalized-3-3,moments_central-0-0,moments_central-0-1,moments_central-0-2,moments_central-0-3,moments_central-1-0,moments_central-1-1,moments_central-1-2,moments_central-1-3,moments_central-2-0,moments_central-2-1,moments_central-2-2,moments_central-2-3,moments_central-3-0,moments_central-3-1,moments_central-3-2,moments_central-3-3,bbox-0,bbox-1,bbox-2,bbox-3,Dia_Ph3_CV,test_channel_indipendent_metric,velocity_pixel,velocity_um,disappears_before_end
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-2,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-3,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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-7,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-7,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-8,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-8,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-9,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-9,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-10,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-10,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-11,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-11,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-12,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-12,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-13,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-13,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-14,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-14,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-15,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-15,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-16,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-16,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-17,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-17,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-18,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-18,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-19,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-19,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-20,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-20,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-21,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-21,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-22,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-22,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-23,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-23,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-24,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-24,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-25,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-25,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-26,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-26,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-27,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-27,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-28,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-28,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-29,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-29,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-30,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-30,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-31,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-31,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-32,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-32,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-33,1,S,0.0,2.0,bud,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-33,2,S,2.0,1.0,mother,-1.0,-1.0,False,-1.0,1.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-34,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-34,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-35,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-35,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-36,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-36,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-37,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-37,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-38,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-38,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-39,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-39,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-40,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-40,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-41,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-41,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-42,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-42,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-43,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-43,2,G1,3.0,1.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
-44,1,G1,1.0,2.0,mother,-1.0,34.0,False,-1.0,0.0,0.0,0.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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