I'm trying out the mvTCR package, and it seems really interesting! I have a dataset with RNA and CITE-seq data, and I've seen that integration using Harmony has really improved the data quality when using Seurat on these modalities. I saw in your paper that you inputted Harmony PCs to mvTCR, and I'd like to try that with my data. Is there any preprocessing or scaling required before using the PC values? How many PCs would you recommend inputting? Thanks!
I'm trying out the mvTCR package, and it seems really interesting! I have a dataset with RNA and CITE-seq data, and I've seen that integration using Harmony has really improved the data quality when using Seurat on these modalities. I saw in your paper that you inputted Harmony PCs to mvTCR, and I'd like to try that with my data. Is there any preprocessing or scaling required before using the PC values? How many PCs would you recommend inputting? Thanks!