issue with FEAST matrix

[lorenzovergani89]

Dear FEST group,
I am traying to use FEAST with a dataset that includes multiple sinks (A, B and C, each of them with replicate samples) teach tested with distinct sources (a different one with replicate samples for each set of sinks). I set different_sources_flag = 1, but I guess I did something wrong when preparing the metadata and count imput table, as I get the following errors:

Error in if (sum(x[i, ]) > maxdepth) { :
missing value where TRUE/FALSE needed
In addition: Warning messages:
1: In matrix(sinks, nrow = 1, ncol = dim(totalsource)[2]) :
data length differs from size of matrix: [32736 != 1 x 1364]
2: In matrix(sinks, nrow = 1, ncol = dim(totalsource)[2]) :
data length differs from size of matrix: [32736 != 1 x 1364]
3: In matrix(sinks, nrow = 1, ncol = dim(totalsource)[2]) :
data length differs from size of matrix: [16368 != 1 x 1364]

Any suggestions?

[davve2]

Hi,

I have the same problem running FEAST, any solution to this issue?

[lorenzovergani89]

Hi,
I solved my problem by giving to each sink a different ID number, as I was told that even if they are replicates of each other, FEAST doesn’t work if there are two sinks with the same ID. I do not know if this applies to your case though.

[Weilan2011]

Hi, I solved my problem by giving to each sink a different ID number, as I was told that even if they are replicates of each other, FEAST doesn’t work if there are two sinks with the same ID. I do not know if this applies to your case though.

What about the sources? I assume you used different sources for your sinks as well? Should we assign difference source IDs to the same source-sampleIDS based on the sink ID changes?