diff --git a/all_local_packages.txt b/all_local_packages.txt
index 830f5c96..5d368c30 100644
--- a/all_local_packages.txt
+++ b/all_local_packages.txt
@@ -5,3 +5,4 @@
 -e ./pytest-embedded-jtag/
 -e ./pytest-embedded-qemu/
 -e ./pytest-embedded-arduino/
+-e ./pytest-embedded-nuttx/
diff --git a/pytest-embedded-nuttx/pytest_embedded_nuttx/app.py b/pytest-embedded-nuttx/pytest_embedded_nuttx/app.py
index 467712a2..4557b487 100644
--- a/pytest-embedded-nuttx/pytest_embedded_nuttx/app.py
+++ b/pytest-embedded-nuttx/pytest_embedded_nuttx/app.py
@@ -22,9 +22,9 @@ def __init__(
         super().__init__(**kwargs)
 
         self.file_extension = file_extension
-        self.app_file, self.bootloader_file, self.merge_file = self._get_bin_files()
+        self.app_file, self.bootloader_file, self.merge_file, self.vefuse_file = self._get_bin_files()
 
-    def _get_bin_files(self) -> tuple[Path | None, Path | None, Path | None]:
+    def _get_bin_files(self) -> tuple[Path | None, Path | None, Path | None, Path | None]:
         """
         Get path to binary files available in the app_path.
         If either the application image or bootloader is not found,
@@ -37,6 +37,7 @@ def _get_bin_files(self) -> tuple[Path | None, Path | None, Path | None]:
         search_pattern = '*' + self.file_extension
         bin_files = list(search_path.rglob(search_pattern))
         app_file, bootloader_file, merge_file = None, None, None
+        vefuse_file = None
 
         logging.info('Searching %s', str(search_path))
         if not bin_files:
@@ -44,6 +45,9 @@ def _get_bin_files(self) -> tuple[Path | None, Path | None, Path | None]:
 
         for file_path in bin_files:
             file_name = str(file_path.stem)
+            if 'vefuse' in file_name:
+                vefuse_file = file_path
+                logging.info('Virtual E-Fuse file: %s', vefuse_file.as_posix())
             if 'nuttx' in file_name:
                 if 'merged' in file_name:
                     merge_file = file_path
@@ -59,4 +63,4 @@ def _get_bin_files(self) -> tuple[Path | None, Path | None, Path | None]:
             logging.error('App file not found: %s', app_file)
             print(bin_files)
 
-        return app_file, bootloader_file, merge_file
+        return app_file, bootloader_file, merge_file, vefuse_file
diff --git a/pytest-embedded-nuttx/pytest_embedded_nuttx/serial.py b/pytest-embedded-nuttx/pytest_embedded_nuttx/serial.py
index 5a6d50b0..f3942fbf 100644
--- a/pytest-embedded-nuttx/pytest_embedded_nuttx/serial.py
+++ b/pytest-embedded-nuttx/pytest_embedded_nuttx/serial.py
@@ -15,7 +15,8 @@ class NuttxSerial(EspSerial):
 
     # Default offset for the primary MCUBoot slot across
     # all Espressif devices on NuttX
-    MCUBOOT_PRIMARY_SLOT_OFFSET = 0x10000
+    MCUBOOT_PRIMARY_SLOT_OFFSET = 0x20000
+    VIRTUAL_EFUSE_OFFSET = 0x10000
     SERIAL_BAUDRATE = 115200
 
     binary_offsets: ClassVar[dict[str, int]] = {
@@ -82,13 +83,19 @@ def get_key_from_value(dictionary, val):
             self.flash_mode = 'dio'
 
     @EspSerial.use_esptool()
-    def flash(self) -> None:
+    def flash(
+        self,
+        virtual_efuse_offset: int = VIRTUAL_EFUSE_OFFSET,
+        mcuboot_primary_slot_offset: int = MCUBOOT_PRIMARY_SLOT_OFFSET,
+    ) -> None:
         """Flash the binary files to the board."""
 
         flash_files = []
         if self.app.bootloader_file:
+            if self.app.vefuse_file:
+                flash_files.extend((str(virtual_efuse_offset), self.app.vefuse_file.as_posix()))
             flash_files.extend((str(self.binary_offsets[self.target]), self.app.bootloader_file.as_posix()))
-            flash_files.extend((str(self.MCUBOOT_PRIMARY_SLOT_OFFSET), self.app.app_file.as_posix()))
+            flash_files.extend((str(mcuboot_primary_slot_offset), self.app.app_file.as_posix()))
         else:
             flash_files.extend((str(self.binary_offsets[self.target]), self.app.app_file.as_posix()))
 
@@ -101,6 +108,11 @@ def flash(self) -> None:
             self.flash_freq,
         ]
 
+        print(f'Flash files: {flash_files}')
+        print(f'Flash settings: {flash_settings}')
+        print(f'Virtual E-Fuse offset: {hex(virtual_efuse_offset)}')
+        print(f'MCUBoot primary slot offset: {hex(mcuboot_primary_slot_offset)}')
+
         esptool.main(
             [
                 '--chip',
diff --git a/pytest-embedded-nuttx/tests/test_nuttx.py b/pytest-embedded-nuttx/tests/test_nuttx.py
index 6383f3b6..8d6a3d2e 100644
--- a/pytest-embedded-nuttx/tests/test_nuttx.py
+++ b/pytest-embedded-nuttx/tests/test_nuttx.py
@@ -48,6 +48,7 @@ def test_nuttx_app_mcuboot(dut, app, target):
             assert '4MB' == dut.serial.flash_size
             assert 'dio' == dut.serial.flash_mode
             assert None != app.bootloader_file
+            assert None == app.vefuse_file
 
         def test_nuttx_app_mcuboot_flash(serial, dut):
             serial.erase_flash()
@@ -73,6 +74,47 @@ def test_hello_mcuboot(dut):
     result.assert_outcomes(passed=3)
 
 
+def test_nuttx_app_mcuboot_with_virtual_efuse(testdir):
+    """This test takes vefuse.bin in consideration. This is a scenario
+    where the bootloader is flashed with virtual efuse enabled, which
+    changes the location in flash for MCUBoot and application.
+    This was added to NuttX on september 2025.
+    """
+    testdir.makepyfile("""
+        import pytest
+
+        def test_nuttx_app_mcuboot(dut, app, target):
+            assert 'esp32' == target
+            assert '40m' == dut.serial.flash_freq
+            assert '4MB' == dut.serial.flash_size
+            assert 'dio' == dut.serial.flash_mode
+            assert None != app.bootloader_file
+            assert None != app.vefuse_file
+
+        def test_nuttx_app_mcuboot_flash(serial, dut):
+            serial.erase_flash()
+            serial.flash()
+            dut.reset_to_nsh()
+
+        def test_hello_mcuboot(dut):
+            dut.reset_to_nsh()
+            dut.write("ls /dev")
+            dut.expect("console")
+    """)
+
+    result = testdir.runpytest(
+        '-s',
+        '--embedded-services',
+        'nuttx,esp',
+        '--target',
+        'esp32',
+        '--app-path',
+        os.path.join(testdir.tmpdir, 'hello_world_nuttx_virtual_efuse'),
+    )
+
+    result.assert_outcomes(passed=3)
+
+
 qemu_bin_required = pytest.mark.skipif(
     shutil.which('qemu-system-xtensa') is None,
     reason='Please make sure that `qemu-system-xtensa` is in your PATH env var. Build QEMU for ESP32 locally and then '
diff --git a/tests/fixtures/hello_world_nuttx_mcuboot/mcuboot-esp32.bin b/tests/fixtures/hello_world_nuttx_mcuboot/mcuboot-esp32.bin
index e376cd18..50cd06c1 100644
Binary files a/tests/fixtures/hello_world_nuttx_mcuboot/mcuboot-esp32.bin and b/tests/fixtures/hello_world_nuttx_mcuboot/mcuboot-esp32.bin differ
diff --git a/tests/fixtures/hello_world_nuttx_mcuboot/nuttx.bin b/tests/fixtures/hello_world_nuttx_mcuboot/nuttx.bin
index 02b6a0af..2c2f85e5 100644
Binary files a/tests/fixtures/hello_world_nuttx_mcuboot/nuttx.bin and b/tests/fixtures/hello_world_nuttx_mcuboot/nuttx.bin differ
diff --git a/tests/fixtures/hello_world_nuttx_virtual_efuse/mcuboot-esp32.bin b/tests/fixtures/hello_world_nuttx_virtual_efuse/mcuboot-esp32.bin
new file mode 100644
index 00000000..50cd06c1
Binary files /dev/null and b/tests/fixtures/hello_world_nuttx_virtual_efuse/mcuboot-esp32.bin differ
diff --git a/tests/fixtures/hello_world_nuttx_virtual_efuse/nuttx.bin b/tests/fixtures/hello_world_nuttx_virtual_efuse/nuttx.bin
new file mode 100644
index 00000000..7a1deae5
Binary files /dev/null and b/tests/fixtures/hello_world_nuttx_virtual_efuse/nuttx.bin differ
diff --git a/tests/fixtures/hello_world_nuttx_virtual_efuse/vefuse.bin b/tests/fixtures/hello_world_nuttx_virtual_efuse/vefuse.bin
new file mode 100644
index 00000000..e69de29b