From 111abd1bb4f62136c38258e0ab2bbfb0ac91db2d Mon Sep 17 00:00:00 2001 From: "Mitchell R. Vollger" Date: Thu, 13 Aug 2026 09:35:19 -0700 Subject: [PATCH 1/5] fix: overlay FIRE quals in qc and restore LINKER decorators for non-FIRE MSPs Same bug class as the fire --extract fix: since the MA-spec refactor (0.10), FIRE quals live on the `fire` annotation type, not the MSPs. `ft qc --m6a-per-msp` read the MSP qual directly, so is_fire was always false, and `ft track-decorators` iterated only fire-type annotations, so precision-0 MSPs (the majority) lost their LINKER decorations relative to 0.6 output. Factor the overlay into FiberseqData::msp_fire_quals() and use it in all three consumers (extract, qc, decorator). Co-Authored-By: Claude Fable 5 --- src/fiber.rs | 19 +++ src/subcommands/decorator.rs | 18 +-- src/subcommands/fire.rs | 18 +-- src/subcommands/qc.rs | 9 +- tests/regression.rs | 2 + tests/regression/decorator.rs | 35 ++++++ tests/regression/fire.rs | 7 ++ tests/regression/qc.rs | 26 ++++ ...track_decorators_emit_fire_and_linker.snap | 111 ++++++++++++++++++ ..._fire__fire_extract_reports_fire_fdrs.snap | 25 ++++ ...qc__qc_m6a_per_msp_sees_fire_elements.snap | 25 ++++ 11 files changed, 266 insertions(+), 29 deletions(-) create mode 100644 tests/regression/decorator.rs create mode 100644 tests/regression/snapshots/regression__decorator__track_decorators_emit_fire_and_linker.snap create mode 100644 tests/regression/snapshots/regression__fire__fire_extract_reports_fire_fdrs.snap create mode 100644 tests/regression/snapshots/regression__qc__qc_m6a_per_msp_sees_fire_elements.snap diff --git a/src/fiber.rs b/src/fiber.rs index 7b16bfb7..5f662d10 100644 --- a/src/fiber.rs +++ b/src/fiber.rs @@ -157,6 +157,25 @@ impl FiberseqData { AnnotationTypeView::new(&self.annotations, FIRE_TYPE) } + /// Per-MSP FIRE quals, BAM-orient ascending (aligned with the `msp()` + /// view's accessors). FIRE quals live on the `fire` annotation type (the + /// subset of MSPs called as FIREs), not on the MSPs themselves — overlay + /// them onto the matching MSPs, keyed by BAM-orient query start (fire + /// entries are exact interval copies of their source MSPs). MSPs without + /// a fire entry fall back to their own qual, which is 0 for MA-era + /// records (FDR 100) and the legacy `aq` value for pre-MA BAMs. + pub fn msp_fire_quals(&self) -> Vec { + let fire = self.fire(); + let fire_quals: std::collections::HashMap = + fire.starts().into_iter().zip(fire.qual()).collect(); + let msp = self.msp(); + msp.qual() + .into_iter() + .zip(msp.starts()) + .map(|(q, s)| fire_quals.get(&s).copied().unwrap_or(q)) + .collect() + } + /// Flush `self.annotations` onto the record's MA-family aux tags. The /// single write path for subcommands that edit nuc/msp/fire annotations; /// call this, then hand the record to the BAM writer. diff --git a/src/subcommands/decorator.rs b/src/subcommands/decorator.rs index f9208b67..ba377efa 100644 --- a/src/subcommands/decorator.rs +++ b/src/subcommands/decorator.rs @@ -166,12 +166,13 @@ pub fn fire_decorators(fiber: &FiberseqData) -> Vec> { map.insert(color, vec![]); } - // FIRE is its own annotation type — color decorators by FIRE precision - // directly. Non-FIRE MSPs are not decorated here - let fire = fiber.fire(); - let ref_starts = fire.reference_starts(); - let ref_lengths = fire.reference_lengths(); - let quals = fire.qual(); + // FIRE quals live on the `fire` annotation type — overlay them onto the + // MSPs so every MSP is decorated: called FIREs by their precision, and + // non-FIRE MSPs as LINKER (qual 0 -> FDR 100), matching pre-MA output. + let msp = fiber.msp(); + let ref_starts = msp.reference_starts(); + let ref_lengths = msp.reference_lengths(); + let quals = fiber.msp_fire_quals(); for ((pos, length), qual) in ref_starts.iter().zip(ref_lengths.iter()).zip(quals.iter()) { if let (Some(p), Some(l)) = (pos, length) { @@ -182,8 +183,11 @@ pub fn fire_decorators(fiber: &FiberseqData) -> Vec> { map.get_mut(&fire_color).unwrap().push((p, l)); } } + // iterate in FIRE_COLORS order so output is deterministic (HashMap + // iteration order is randomized per process) let mut rtn = vec![]; - for (color, values) in map.into_iter() { + for color in FIRE_COLORS.iter().map(|(_, color)| color) { + let values = map.remove(&color).unwrap(); let (starts, lengths): (Vec>, Vec>) = values.into_iter().unzip(); let el_type = if *color == LINKER_COLOR { "LINKER" diff --git a/src/subcommands/fire.rs b/src/subcommands/fire.rs index 37fa801a..d33cc87d 100644 --- a/src/subcommands/fire.rs +++ b/src/subcommands/fire.rs @@ -128,23 +128,7 @@ pub fn fire_to_bed9(fire_opts: &FireOptions, bam: &mut bam::Reader) -> Result<() let msp_ends = msp.reference_ends(); let nuc_ends = nuc.reference_ends(); let end_iter = msp_ends.iter().chain(nuc_ends.iter()); - // FIRE quals live on the `fire` annotation type (the subset of MSPs - // called as FIREs), not on the MSPs themselves. Overlay them onto the - // matching MSPs (keyed by molecular start) so extraction reports the - // called FDR; MSPs without a fire entry fall back to their own qual - // (nonzero only for legacy-tag BAMs) and so report an FDR of 100. - let fire = rec.fire(); - let fire_quals: std::collections::HashMap = fire - .starts() - .into_iter() - .zip(fire.qual().into_iter()) - .collect(); - let msp_qual: Vec = msp - .qual() - .into_iter() - .zip(msp.starts().into_iter()) - .map(|(q, s)| fire_quals.get(&s).copied().unwrap_or(q)) - .collect(); + let msp_qual = rec.msp_fire_quals(); let nuc_qual = nuc.qual(); let qual_iter = msp_qual.iter().chain(nuc_qual.iter()); let n_msps = msp_starts.len(); diff --git a/src/subcommands/qc.rs b/src/subcommands/qc.rs index e76fb0d2..587766c6 100644 --- a/src/subcommands/qc.rs +++ b/src/subcommands/qc.rs @@ -243,13 +243,12 @@ impl<'a> QcStats<'a> { fn m6a_per_msp(&mut self, fiber: &fiber::FiberseqData) { let msp = fiber.msp(); let m6a_starts = fiber.m6a().starts(); - for annotation in &msp { + // FIRE quals live on the `fire` annotation type, not the MSPs — + // msp_fire_quals() overlays them (both are BAM-orient ascending). + let quals = fiber.msp_fire_quals(); + for (annotation, qual) in msp.infos().iter().zip(quals.into_iter()) { let st = annotation.query_start as i64; let en = annotation.query_end as i64; - let qual = crate::utils::bamannotations::primary_qual( - annotation.qualities, - annotation.type_name, - ); let is_fire = qual >= 230; let msp_size = en - st; let m6a_count = m6a_starts diff --git a/tests/regression.rs b/tests/regression.rs index 448d614a..ffcb41be 100644 --- a/tests/regression.rs +++ b/tests/regression.rs @@ -4,6 +4,8 @@ mod center; mod common; #[path = "regression/convert_tags.rs"] mod convert_tags; +#[path = "regression/decorator.rs"] +mod decorator; #[path = "regression/extract.rs"] mod extract; #[path = "regression/fire.rs"] diff --git a/tests/regression/decorator.rs b/tests/regression/decorator.rs new file mode 100644 index 00000000..3d3bebc7 --- /dev/null +++ b/tests/regression/decorator.rs @@ -0,0 +1,35 @@ +use super::common::{fixture, run}; +use tempfile::NamedTempFile; + +// Every MSP must produce a decoration: called FIREs by their precision and +// non-FIRE (precision-0) MSPs as LINKER, matching pre-MA output. FIRE quals +// live on the `fire` annotation type, so the decorator must overlay them +// onto the MSPs rather than iterate the fire type alone. +#[test] +fn track_decorators_emit_fire_and_linker() { + let scored = NamedTempFile::with_suffix(".bam").unwrap(); + run(&[ + "fire", + fixture("all.bam").to_str().unwrap(), + scored.path().to_str().unwrap(), + ]); + let bed12 = NamedTempFile::with_suffix(".bed").unwrap(); + let out = run(&[ + "track-decorators", + "--bed12", + bed12.path().to_str().unwrap(), + scored.path().to_str().unwrap(), + ]); + let count = |el: &str| { + out.lines() + .filter(|l| l.split('\t').any(|f| f == el)) + .count() + }; + let (fire, linker) = (count("FIRE"), count("LINKER")); + assert!(fire > 0, "no FIRE decorations emitted"); + assert!( + linker > fire, + "expected precision-0 MSPs (the majority) to decorate as LINKER; got {linker} LINKER vs {fire} FIRE" + ); + insta::assert_snapshot!(out); +} diff --git a/tests/regression/fire.rs b/tests/regression/fire.rs index 8b38e492..554112b9 100644 --- a/tests/regression/fire.rs +++ b/tests/regression/fire.rs @@ -24,6 +24,13 @@ fn fire_extract_reports_fire_fdrs() { fdrs.iter().any(|&f| f < 1.0), "no FIRE elements with FDR < 1.0 in extract output; fire quals were not overlaid onto MSPs" ); + // Pin the called FIRE elements (FDR < 1) so qual->FDR mapping drift is caught. + let fire_rows: String = out + .lines() + .filter(|l| l.split('\t').nth(9).unwrap().parse::().unwrap() < 1.0) + .collect::>() + .join("\n"); + insta::assert_snapshot!(fire_rows); } // Snapshot a stable subset of FIRE feature columns so additions/reorderings diff --git a/tests/regression/qc.rs b/tests/regression/qc.rs index 6d36e7d3..ac4cb5cc 100644 --- a/tests/regression/qc.rs +++ b/tests/regression/qc.rs @@ -1,7 +1,33 @@ use super::common::{fixture, run, select_tsv_cols}; +use tempfile::NamedTempFile; #[test] fn qc_default() { let out = run(&["qc", fixture("all.bam").to_str().unwrap()]); insta::assert_snapshot!(select_tsv_cols(&out, &["statistic", "value", "count"])); } + +// FIRE quals live on the `fire` annotation type (MA spec), not the MSPs; +// the m6a_per_msp_size statistic must overlay them or is_fire is always +// false for fire-scored BAMs. +#[test] +fn qc_m6a_per_msp_sees_fire_elements() { + let scored = NamedTempFile::with_suffix(".bam").unwrap(); + run(&[ + "fire", + fixture("all.bam").to_str().unwrap(), + scored.path().to_str().unwrap(), + ]); + let out = run(&["qc", "-m", scored.path().to_str().unwrap()]); + let fire_rows: Vec<&str> = out + .lines() + .filter(|l| { + l.starts_with("m6a_per_msp_size") && l.split('\t').nth(1).unwrap().ends_with(",true") + }) + .collect(); + assert!( + !fire_rows.is_empty(), + "no m6a_per_msp_size rows with is_fire=true; fire quals were not overlaid onto MSPs" + ); + insta::assert_snapshot!(fire_rows.join("\n")); +} diff --git a/tests/regression/snapshots/regression__decorator__track_decorators_emit_fire_and_linker.snap b/tests/regression/snapshots/regression__decorator__track_decorators_emit_fire_and_linker.snap new file mode 100644 index 00000000..2e1add7c --- /dev/null +++ b/tests/regression/snapshots/regression__decorator__track_decorators_emit_fire_and_linker.snap @@ -0,0 +1,111 @@ +--- +source: tests/regression/decorator.rs +expression: out +--- +ptg000001l 318 15592 m6A 0 - 318 15592 128,0,128,1 1526 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,8,13,16,148,154,166,171,299,301,311,314,316,318,323,326,327,328,330,334,337,338,340,342,343,345,346,348,349,350,352,354,355,356,359,360,362,365,366,367,370,371,373,375,377,378,497,499,504,512,518,521,522,523,525,526,529,530,532,533,535,537,538,539,544,551,557,585,588,591,600,606,608,725,737,739,741,746,748,749,753,754,755,756,757,758,760,761,762,763,764,766,767,769,771,772,774,777,780,782,784,790,795,801,802,803,807,817,819,820,826,827,845,846,848,850,852,856,867,989,991,994,996,1002,1005,1007,1013,1016,1028,1030,1032,1039,1042,1044,1045,1190,1195,1201,1205,1207,1219,1223,1335,1341,1343,1347,1349,1351,1355,1359,1363,1365,1368,1370,1372,1375,1378,1381,1383,1386,1388,1392,1394,1398,1402,1403,1405,1407,1415,1419,1421,1423,1425,1427,1429,1437,1439,1440,1443,1447,1450,1453,1455,1458,1461,1463,1479,1494,1498,1583,1585,1591,1593,1597,1599,1600,1601,1604,1606,1608,1611,1614,1616,1620,1621,1622,1625,1626,1633,1638,1640,1642,1644,1646,1648,1654,1665,1666,1765,1772,1773,1777,1779,1780,1786,1790,1793,1795,1797,1798,1802,1803,1807,1808,1813,1814,1817,1819,1821,1824,1825,1827,1830,1833,1834,1836,1839,1841,1845,1847,1851,1854,1858,1973,1990,1992,1993,1999,2005,2011,2018,2023,2024,2026,2027,2035,2039,2043,2045,2047,2150,2151,2166,2192,2197,2199,2200,2202,2205,2206,2214,2217,2222,2225,2228,2230,2233,2340,2344,2346,2348,2350,2352,2354,2361,2362,2367,2372,2374,2376,2378,2379,2382,2384,2386,2388,2389,2391,2396,2397,2403,2406,2408,2409,2411,2413,2416,2428,2429,2557,2560,2561,2563,2569,2571,2572,2574,2577,2578,2580,2582,2585,2594,2597,2600,2607,2608,2610,2611,2612,2615,2616,2617,2637,2658,2661,2745,2748,2758,2766,2772,2774,2776,2783,2785,2884,2897,2908,2910,2914,2915,2920,2921,2924,2925,2927,2929,2932,2934,2936,2938,2946,2948,2950,2952,2953,2954,2956,2957,2959,2961,2963,2964,2967,2969,2971,2972,2973,2977,2982,2987,2994,3019,3135,3143,3147,3149,3150,3151,3153,3154,3158,3159,3161,3163,3164,3165,3167,3168,3170,3173,3185,3187,3190,3191,3193,3196,3256,3310,3313,3315,3322,3327,3329,3333,3337,3344,3345,3348,3349,3352,3354,3366,3367,3370,3372,3373,3374,3377,3380,3382,3386,3387,3388,3390,3391,3413,3417,3420,3421,3424,3546,3550,3552,3553,3554,3559,3569,3571,3573,3591,3592,3593,3595,3597,3598,3604,3606,3608,3611,3613,3614,3615,3620,3736,3739,3742,3745,3751,3752,3755,3756,3758,3761,3762,3764,3767,3770,3771,3773,3778,3779,3781,3785,3788,3790,3792,3795,3804,3807,3808,3813,3814,3816,3819,3830,3840,3845,3850,3916,3924,3939,3942,3944,3945,3947,3961,3965,3966,3970,3975,3978,3981,3984,3989,3993,3996,3998,3999,4001,4005,4015,4103,4116,4133,4136,4138,4139,4142,4144,4149,4151,4153,4154,4155,4160,4165,4167,4169,4171,4174,4312,4334,4337,4459,4467,4480,4484,4485,4487,4491,4492,4496,4497,4503,4505,4509,4512,4515,4516,4531,4532,4535,4636,4650,4664,4667,4669,4670,4671,4675,4680,4681,4685,4686,4689,4691,4692,4694,4697,4702,4705,4707,4711,4713,4716,4717,4720,4724,4732,4742,4744,4810,4823,4826,4831,4841,4842,4848,4851,4855,4857,4860,4864,4866,4867,4871,4874,4876,4879,4881,4884,4888,4892,4896,4898,4902,4904,4909,4910,5068,5070,5083,5086,5088,5089,5090,5092,5094,5095,5098,5100,5103,5105,5107,5110,5124,5138,5144,5282,5287,5289,5293,5294,5305,5307,5311,5316,5328,5339,5439,5459,5478,5480,5631,5643,5644,5647,5649,5652,5654,5655,5664,5667,5813,5817,5819,5827,5828,5832,5834,5838,5840,5841,5847,5848,5850,5854,5857,5858,5863,5978,5982,5983,5985,5989,5992,5993,5998,6002,6011,6017,6018,6020,6022,6152,6154,6155,6158,6164,6171,6176,6179,6192,6194,6199,6201,6202,6206,6208,6211,6212,6215,6216,6221,6229,6233,6235,6236,6240,6273,6279,6348,6354,6357,6358,6362,6364,6366,6367,6370,6372,6374,6375,6378,6382,6384,6389,6394,6396,6398,6404,6408,6409,6410,6412,6416,6417,6418,6420,6422,6424,6427,6447,6450,6453,6460,6588,6591,6592,6621,6625,6725,6751,6755,6761,6762,6767,6768,6769,6772,6774,6776,6777,6779,6781,6783,6785,6788,6789,6791,6793,6795,6796,6798,6800,6801,6802,6811,6812,6813,6816,6820,6822,6823,6825,6827,6832,6841,6843,6846,6848,6852,6860,6978,6993,6998,7127,7129,7131,7138,7141,7152,7153,7154,7155,7159,7172,7179,7325,7328,7331,7334,7336,7340,7341,7473,7474,7476,7480,7482,7484,7485,7487,7489,7494,7501,7503,7505,7507,7508,7617,7624,7642,7644,7645,7648,7650,7653,7656,7658,7661,7666,7667,7669,7675,7680,7685,7687,7688,7692,7694,7702,7705,7803,7806,7808,7829,7835,7847,7848,7850,7852,7854,7857,7859,7861,7863,7864,7867,7869,7871,7873,7876,7877,7879,7882,7884,7888,7894,7898,7900,7906,7908,7911,7914,7918,7920,7924,7926,7933,8076,8077,8080,8083,8087,8090,8092,8095,8096,8097,8098,8102,8105,8107,8109,8111,8112,8114,8119,8122,8125,8127,8130,8138,8179,8182,8184,8234,8273,8277,8279,8282,8284,8285,8286,8288,8290,8298,8300,8301,8304,8305,8306,8315,8316,8319,8322,8324,8326,8328,8329,8334,8336,8340,8342,8346,8348,8372,8472,8474,8478,8491,8492,8495,8497,8500,8503,8505,8510,8511,8520,8523,8534,8653,8658,8660,8662,8664,8667,8669,8671,8672,8673,8854,8857,8860,8862,8874,8875,8891,8894,8897,8899,9028,9033,9034,9039,9041,9045,9046,9057,9064,9066,9074,9080,9085,9093,9210,9214,9223,9230,9238,9362,9377,9380,9382,9384,9386,9387,9389,9393,9395,9397,9407,9540,9542,9555,9557,9567,9569,9572,9574,9577,9578,9580,9585,9586,9587,9595,9607,9610,9613,9616,9618,9621,9624,9713,9743,9746,9748,9752,9755,9758,9761,9762,9764,9769,9772,9774,9776,9778,9780,9782,9784,9786,9787,9790,9792,9795,9797,9799,9801,9802,9803,9805,9806,9809,9811,9814,9825,9827,9882,9952,9954,9955,9958,9959,9963,9975,9979,9990,9992,9998,10000,10001,10003,10129,10133,10138,10143,10145,10148,10152,10157,10158,10162,10163,10169,10171,10173,10174,10179,10206,10315,10318,10330,10335,10342,10345,10354,10359,10363,10379,10380,10383,10384,10506,10523,10527,10530,10531,10534,10691,10693,10694,10696,10698,10701,10704,10709,10720,10722,10723,10726,10729,10735,10737,10759,10761,10763,10765,10882,10891,10893,10895,10897,10900,10902,10913,11061,11064,11072,11076,11082,11084,11152,11209,11224,11228,11235,11238,11244,11249,11402,11407,11412,11558,11561,11571,11573,11576,11578,11589,11593,11595,11598,11602,11603,11605,11612,11614,11616,11617,11619,11621,11622,11625,11626,11627,11632,11636,11757,11908,11915,11938,11942,11945,11947,11948,11951,11952,11960,11963,12079,12086,12122,12124,12141,12143,12152,12154,12160,12162,12166,12168,12169,12275,12286,12291,12298,12301,12303,12304,12307,12309,12310,12313,12316,12321,12322,12326,12332,12340,12342,12348,12353,12357,12372,12484,12487,12488,12492,12508,12511,12514,12517,12522,12524,12642,12649,12663,12664,12667,12674,12677,12679,12682,12683,12688,12689,12692,12696,12703,12706,12707,12709,12712,12716,12717,12719,12722,12724,12726,12727,12729,12853,12856,12859,12860,12862,12870,12874,12878,12880,12887,12889,12891,12898,12899,12905,12909,12911,12913,12916,12917,12923,12928,12939,13052,13054,13056,13059,13066,13071,13093,13247,13318,13384,13386,13392,13400,13403,13412,13413,13415,13431,13433,13556,13559,13605,13608,13610,13612,13615,13621,13638,13643,13644,13646,13648,13650,13652,13654,13656,13668,13675,13680,13690,13816,13818,13820,13822,13823,13825,13830,13833,13837,13840,13844,13846,13848,13850,13854,13858,13861,13871,13874,13876,13878,13882,13886,13891,13894,14013,14024,14027,14030,14031,14032,14035,14036,14038,14041,14044,14051,14060,14061,14064,14066,14067,14070,14073,14076,14078,14080,14082,14084,14086,14088,14090,14097,14161,14216,14221,14225,14227,14230,14239,14241,14244,14247,14248,14250,14252,14256,14258,14260,14261,14266,14311,14321,14403,14405,14406,14407,14409,14414,14420,14425,14427,14432,14434,14437,14439,14442,14449,14451,14454,14456,14458,14466,14496,14589,14595,14596,14598,14610,14624,14626,14628,14632,14641,14643,14660,14661,14663,14667,14669,14673,14677,14691,14794,14804,14806,14811,14813,14815,14817,14822,14825,14827,14830,14833,14834,14836,14839,14842,14845,14849,14858,14863,14864,14865,14870,14871,14874,14875,14876,14883,14885,14889,14905,15006,15045,15047,15049,15069,15189,15193,15196,15197,15202,15205,15206,15208,15209,15221,15223,15224,15226,15256,15267,15270,15272,15273, ptg000001l:306-15696:m54329U_210323_190418/5048829/ccs block 128,0,128,0 Ignored m6A +ptg000001l 812 15047 5mC 0 - 812 15047 139,69,19,1 38 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,744,750,980,1010,2037,2142,2181,2194,2213,2324,2405,2724,2727,3170,4236,4407,5436,7043,8046,8635,8673,8675,8681,9159,9610,9923,10127,10818,11555,11799,11803,12843,13239,13605,13700,14182,14234, ptg000001l:306-15696:m54329U_210323_190418/5048829/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 490 15507 NUC 0 - 490 15507 169,169,169,1 76 127,118,116,121,135,111,84,98,114,102,106,127,83,98,115,113,121,114,87,137,121,100,157,137,99,150,145,114,129,107,127,125,117,128,145,131,108,97,142,99,118,180,128,116,123,132,118,124,125,135,121,156,116,147,124,152,145,120,150,115,105,111,117,123,112,153,136,122,125,118,118,81,122,102,139,119, 0,207,437,696,874,1052,1327,1495,1687,1876,2062,2258,2490,2614,2848,3025,3253,3450,3844,4003,4166,4364,4739,4973,5168,5309,5496,5692,5851,6069,6289,6454,6689,6827,7008,7170,7337,7534,7762,8201,8363,8502,8728,8922,9067,9236,9453,9656,9832,10008,10213,10363,10594,10742,10913,11078,11241,11465,11586,11792,11998,12201,12353,12558,12768,12922,13076,13262,13519,13723,13926,14150,14295,14520,14734,14898, ptg000001l:306-15696:m54329U_210323_190418/5048829/ccs block 169,169,169,0 Ignored NUC +ptg000001l 1653 1817 FIRE 0 - 1653 1817 225,0,0,1 1 164, 0, ptg000001l:306-15696:m54329U_210323_190418/5048829/ccs block 225,0,0,0 Ignored FIRE +ptg000001l 466 15592 LINKER 0 - 466 15592 147,112,219,1 76 24,80,112,143,57,43,84,94,75,84,90,105,41,136,62,115,76,280,72,26,77,275,77,58,42,37,51,45,89,113,38,110,21,53,17,36,89,131,297,63,21,46,66,29,46,85,85,52,51,70,29,75,32,24,41,11,79,1,56,91,98,41,88,87,42,1,50,135,79,85,106,64,103,112,25,85, 0,151,349,577,841,1033,1435,1617,1825,2002,2192,2409,2597,2736,2987,3162,3398,3588,3955,4164,4311,4488,4920,5134,5291,5483,5665,5830,6004,6200,6440,6603,6830,6979,7177,7325,7469,7655,7928,8324,8505,8706,8880,9062,9214,9392,9595,9804,9981,10167,10358,10543,10734,10913,11061,11254,11410,11609,11760,11931,12127,12336,12494,12705,12904,13099,13236,13408,13668,13865,14068,14255,14441,14646,14897,15041, ptg000001l:306-15696:m54329U_210323_190418/5048829/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 985 21720 m6A 0 + 985 21720 128,0,128,1 318 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,175,291,357,372,375,377,653,740,754,949,955,966,969,1126,1128,1317,1323,1519,1525,1528,1533,1695,2048,2049,2053,2056,2061,2069,2078,2081,2085,2093,2095,2260,2421,2426,2436,2439,2541,2546,2627,2629,2632,2633,2662,2983,2985,2986,3036,3180,3383,3386,3396,3552,3555,3789,3825,3983,3997,4109,4174,4181,4200,4359,4363,4367,4373,4378,4379,4932,4935,4936,4938,4940,4942,5121,5128,5134,5135,5140,5143,5144,5146,5150,5209,5315,5318,5322,5325,5331,5353,5521,5523,5527,5606,5697,5703,5707,5714,6271,6274,6277,6956,6975,6977,6983,6986,7294,7326,7824,7825,7828,7844,7846,7853,7856,8002,8109,8153,8187,8208,8554,8710,8717,8719,8722,8726,8730,8900,8902,8907,8910,9091,9094,9095,9097,9102,9111,9113,9126,9285,9425,9429,9682,9684,9696,9863,10394,10404,10483,10791,11293,11479,11483,11499,11502,11686,11705,11718,11719,11725,11885,11886,11905,11908,12296,12319,12497,12805,12810,12830,12837,12843,12847,12850,13035,13039,13045,13224,13227,13239,13411,13415,13417,13423,13427,13428,13430,13434,13438,13441,13451,13454,13830,13832,13835,13836,13839,13842,13843,13846,13848,13849,13852,13856,14021,14024,14182,14190,14204,14207,14218,14222,14225,14228,14229,14230,14231,14234,14236,14238,14408,14427,14438,14439,14441,14446,14450,14462,14640,14644,14648,14652,14819,14825,14829,14832,14834,14851,14855,14857,14860,14864,14868,14873,14879,14892,14911,14917,15025,15027,15042,15046,15048,15466,15472,15961,15989,15996,16159,16193,16211,16583,16746,16749,16752,16763,16768,16770,16772,16773,16775,16777,16779,16780,16783,16954,17114,17120,17148,17291,17294,17320,17324,17516,17519,17529,17929,18250,18259,18438,18450,18454,18457,18463,18465,18654,18801,18960,18994,18998,19001,19003,19008,19015,19017,19347,19350,19508,19512,19518,19672,19679,19788,20035,20724,20734, ptg000001l:832-21833:m54329U_210323_190418/141691444/ccs block 128,0,128,0 Ignored m6A +ptg000001l 1555 21791 5mC 0 + 1555 21791 139,69,19,1 79 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,6,204,208,236,266,313,717,911,1293,1398,1437,1450,1580,1629,1661,1983,3608,4692,4763,5648,6299,7043,7891,7937,7962,8058,8713,9179,9284,9543,9560,9748,9754,9918,10157,10322,10470,10811,11447,12133,12136,12227,12614,13119,13506,13518,13669,14085,14118,14311,14349,15058,15249,16414,16791,16794,16840,16848,16853,16899,16901,16921,17069,17188,17285,17367,17459,17859,17861,17904,18082,18675,18752,18853,18904,18906,20220,20235, ptg000001l:832-21833:m54329U_210323_190418/141691444/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 986 21709 NUC 0 + 986 21709 169,169,169,1 83 174,181,275,86,194,156,188,195,161,352,164,160,101,80,320,143,202,155,233,157,176,158,552,178,164,167,78,90,556,678,307,497,145,150,345,155,169,180,158,139,252,159,530,78,307,501,185,183,159,387,177,307,184,178,171,375,164,157,169,177,166,107,417,488,162,371,162,170,159,142,191,399,320,178,188,146,158,329,157,153,108,246,688, 0,175,377,653,754,969,1128,1323,1533,1695,2095,2260,2439,2546,2662,3036,3180,3396,3555,3825,3997,4200,4379,4942,5150,5353,5527,5606,5714,6277,6986,7326,7856,8002,8208,8554,8730,8910,9126,9285,9429,9696,9863,10404,10483,10791,11293,11502,11725,11908,12319,12497,12850,13045,13239,13454,13856,14024,14238,14462,14652,14917,15048,15472,15996,16211,16583,16783,16954,17148,17324,17529,17929,18259,18465,18654,18801,19017,19350,19518,19679,19788,20035, ptg000001l:832-21833:m54329U_210323_190418/141691444/ccs block 169,169,169,0 Ignored NUC +ptg000001l 985 21720 LINKER 0 + 985 21720 147,112,219,1 84 1,1,21,1,15,21,3,7,15,1,48,1,19,6,36,54,1,14,4,37,15,27,21,11,30,39,7,1,18,7,31,33,33,1,56,1,21,11,36,1,5,15,8,11,1,1,1,24,40,24,24,1,46,11,16,44,27,4,57,55,13,99,24,7,36,53,1,38,1,35,34,14,1,10,28,1,1,58,4,11,8,1,1,11, 0,175,357,653,740,949,1126,1317,1519,1695,2048,2260,2421,2541,2627,2983,3180,3383,3552,3789,3983,4174,4359,4932,5121,5315,5521,5606,5697,6271,6956,7294,7824,8002,8153,8554,8710,8900,9091,9285,9425,9682,9856,10394,10483,10791,11293,11479,11686,11885,12296,12497,12805,13035,13224,13411,13830,14021,14182,14408,14640,14819,15025,15466,15961,16159,16583,16746,16954,17114,17291,17516,17929,18250,18438,18654,18801,18960,19347,19508,19672,19788,20035,20724, ptg000001l:832-21833:m54329U_210323_190418/141691444/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 6866 20931 m6A 0 - 6866 20931 128,0,128,1 887 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,5,12,16,19,29,40,182,203,207,210,213,226,229,231,351,372,377,383,384,388,390,396,403,455,557,560,561,564,569,571,581,583,590,600,742,777,925,932,936,944,1083,1094,1096,1097,1100,1102,1126,1127,1132,1137,1255,1258,1268,1282,1284,1299,1300,1302,1304,1306,1309,1311,1313,1315,1316,1319,1321,1323,1325,1328,1329,1331,1334,1335,1352,1358,1505,1535,1693,1694,1698,1700,1703,1706,1708,1711,1713,1725,1727,1729,1731,1734,1737,1738,1740,1750,1757,1903,1904,1906,1909,1911,1916,1917,1922,1932,1933,1935,1940,2105,2110,2112,2114,2263,2265,2272,2275,2278,2288,2306,2449,2456,2460,2475,2477,2479,2485,2486,2488,2490,2493,2497,2630,2634,2641,2657,2662,2663,2666,2673,2675,2679,2682,2686,2687,2690,2694,2696,2697,2699,2703,2710,2711,2718,2719,2723,2838,2841,2847,2849,2859,2878,2879,2884,2887,2898,2994,3009,3012,3013,3019,3021,3024,3026,3029,3032,3047,3048,3049,3052,3053,3055,3056,3059,3061,3062,3065,3068,3070,3073,3076,3077,3079,3085,3087,3096,3098,3101,3103,3106,3109,3110,3115,3221,3225,3230,3232,3236,3239,3240,3242,3244,3245,3247,3249,3251,3253,3257,3258,3261,3263,3266,3272,3274,3275,3279,3282,3285,3286,3291,3293,3294,3297,3298,3299,3300,3303,3306,3316,3319,3338,3340,3346,3350,3353,3477,3482,3483,3485,3487,3488,3495,3642,3645,3649,3656,3658,3663,3667,3668,3673,3818,3824,3834,3839,3844,3850,3851,3854,3856,3861,3870,4017,4020,4028,4031,4037,4038,4045,4047,4056,4059,4062,4092,4093,4095,4099,4220,4248,4560,4565,4567,4573,4575,4580,4582,4588,4592,4595,4597,4601,4604,4606,4609,4610,4623,4752,4754,4757,4780,4781,4783,4785,4791,4799,4802,4807,4810,4814,4823,4831,4833,4838,4848,4966,4986,4989,4990,4991,4995,4996,4998,5001,5002,5013,5023,5025,5164,5171,5173,5178,5179,5193,5194,5195,5199,5200,5204,5206,5207,5213,5391,5396,5397,5399,5400,5403,5412,5415,5433,5455,5458,5602,5604,5606,5610,5614,5615,5618,5627,5759,5765,5784,5786,5788,5793,5794,5800,5805,5824,5972,5974,5976,5997,6000,6158,6163,6164,6168,6169,6174,6183,6188,6195,6205,6206,6208,6210,6221,6226,6363,6378,6380,6382,6385,6391,6566,6581,6596,6766,6768,6770,6778,6781,6816,6925,6929,6932,6936,6938,6941,6945,6948,6951,6952,6960,6962,6966,6969,6977,7018,7096,7098,7100,7103,7104,7106,7108,7132,7138,7139,7142,7146,7154,7157,7158,7161,7163,7164,7170,7306,7309,7310,7313,7315,7316,7323,7336,7341,7343,7344,7346,7349,7358,7360,7518,7519,7522,7525,7528,7530,7532,7534,7536,7538,7540,7542,7547,7549,7553,7557,7560,7563,7578,7872,7879,7901,7903,7906,7908,7919,7925,7928,7931,7933,7935,7937,7938,7939,7942,7949,7954,7955,7958,7962,7965,7968,7971,7974,7975,7988,8079,8080,8084,8085,8087,8089,8093,8095,8112,8113,8115,8117,8119,8121,8124,8125,8140,8143,8151,8154,8158,8301,8309,8310,8312,8313,8315,8316,8317,8322,8323,8326,8337,8341,8344,8486,8492,8493,8495,8497,8499,8501,8504,8508,8510,8512,8514,8519,8521,8523,8525,8527,8532,8535,8546,8684,8706,8707,8720,8722,8724,8725,8728,8730,8732,8735,8898,8904,8907,8927,9080,9085,9086,9101,9104,9107,9112,9114,9122,9125,9126,9130,9144,9146,9282,9287,9292,9301,9306,9458,9471,9474,9476,9477,9482,9483,9485,9490,9491,9496,9506,9508,9517,9659,9664,9666,9667,9669,9671,9675,9689,9694,9701,9708,9714,9856,9857,9863,9865,9877,9880,9886,9889,9897,9900,9903,9905,9909,9913,9914,10082,10085,10086,10095,10099,10100,10107,10108,10112,10113,10115,10119,10260,10266,10278,10290,10293,10312,10317,10472,10676,10678,10686,10687,10692,10694,10695,10697,10699,10707,10730,10731,10868,10887,10889,10891,10892,10894,10896,10899,10902,10957,11060,11072,11073,11075,11081,11096,11257,11260,11265,11267,11273,11289,11292,11296,11300,11302,11424,11426,11429,11439,11441,11446,11452,11469,11608,11639,11642,11645,11648,11649,11721,11803,11808,11811,11830,11833,11835,11837,11841,11847,11849,11850,11855,11856,11859,12006,12012,12014,12015,12019,12020,12022,12023,12024,12027,12028,12031,12033,12034,12037,12039,12042,12043,12044,12048,12049,12050,12052,12054,12055,12071,12076,12080,12087,12101,12217,12230,12236,12239,12241,12243,12245,12248,12250,12251,12257,12258,12267,12274,12275,12277,12303,12424,12426,12437,12444,12450,12467,12470,12474,12600,12603,12621,12622,12624,12627,12631,12637,12639,12640,12643,12647,12653,12655,12658,12776,12783,12788,12814,12816,12818,12821,12824,12825,12827,12829,12831,12833,12835,12837,12839,12845,12847,12851,12853,12858,12859,12863,12865,12866,12869,12871,12884,13008,13009,13010,13012,13014,13016,13017,13020,13022,13025,13028,13042,13049,13053,13060,13064,13073,13078,13216,13231,13234,13236,13237,13239,13276,13396,13398,13413,13433,13437,13444,13446,13610,13614,13616,13621,13627,13631,13634,13637,13641,13643,13645,13646,13649,13653,13656,13659,13662,13670,13690,13835,13843,13846,14038,14045,14047,14049,14051,14064, ptg000001l:6745-20968:m54329U_210323_190418/175376495/ccs block 128,0,128,0 Ignored m6A +ptg000001l 7016 20579 5mC 0 - 7016 20579 139,69,19,1 65 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,2431,2469,2471,2477,2630,2660,2811,2836,3253,3406,3658,4288,4294,4458,4532,4614,4912,5010,5351,5467,5488,6639,6767,7035,7401,7425,8046,8058,8121,8156,8714,8889,9061,10010,10362,10954,11331,11334,11337,11380,11388,11393,11415,11423,11439,11441,11461,11467,11478,11484,11504,11886,11907,11962,12163,12399,12401,12542,12622,13112,13215,13292,13446,13562, ptg000001l:6745-20968:m54329U_210323_190418/175376495/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 6907 20904 NUC 0 - 6907 20904 169,169,169,1 70 141,119,153,141,147,138,117,146,156,145,164,148,142,132,114,95,105,123,146,144,146,148,311,128,117,138,177,143,131,147,157,136,174,169,143,117,135,157,293,90,142,141,137,162,152,135,151,141,141,167,140,154,203,136,157,160,121,138,153,146,115,146,125,117,123,137,156,163,144,191, 0,191,363,560,737,904,1097,1318,1496,1717,1900,2074,2266,2457,2683,2858,3075,3313,3455,3633,3830,4059,4208,4583,4808,4985,5173,5418,5587,5784,5960,6186,6351,6556,6741,6938,7130,7320,7538,7948,8118,8304,8506,8695,8887,9106,9266,9477,9674,9874,10079,10277,10432,10691,10862,11056,11262,11429,11609,11819,12061,12237,12434,12618,12844,13038,13199,13406,13650,13806, ptg000001l:6745-20968:m54329U_210323_190418/175376495/ccs block 169,169,169,0 Ignored NUC +ptg000001l 6866 20713 LINKER 0 - 6866 20713 147,112,219,1 70 41,50,53,44,36,20,55,104,31,65,38,10,44,49,94,61,122,133,19,32,53,83,1,64,97,60,50,68,26,66,29,69,29,31,16,53,75,55,61,117,80,44,61,52,30,67,25,60,56,59,38,58,1,56,35,37,46,46,42,57,96,61,51,59,109,71,24,51,81,12, 0,182,351,557,742,925,1083,1255,1505,1693,1903,2105,2263,2449,2630,2838,2994,3221,3477,3642,3818,4017,4248,4560,4752,4966,5164,5391,5602,5759,5972,6158,6363,6566,6766,6925,7096,7306,7518,7872,8079,8301,8486,8684,8898,9080,9282,9458,9659,9856,10082,10260,10472,10676,10868,11060,11257,11424,11608,11803,12006,12217,12424,12600,12776,13008,13216,13396,13610,13835, ptg000001l:6745-20968:m54329U_210323_190418/175376495/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 13567 36176 m6A 0 - 13567 36176 128,0,128,1 1694 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,6,158,167,178,180,182,352,492,496,501,503,504,507,510,515,517,518,521,522,527,530,533,535,541,555,671,683,696,698,701,703,706,708,709,710,712,714,725,727,729,730,732,733,735,736,737,740,748,751,869,879,882,887,889,897,905,908,911,912,917,919,922,924,925,926,929,933,937,943,948,955,1091,1100,1103,1234,1238,1240,1244,1246,1247,1248,1250,1253,1254,1257,1260,1261,1263,1264,1266,1267,1270,1273,1274,1278,1288,1293,1297,1298,1300,1303,1304,1310,1312,1314,1316,1319,1320,1323,1326,1327,1439,1442,1450,1453,1457,1464,1467,1468,1471,1476,1490,1519,1600,1685,1687,1689,1692,1693,1713,1727,1864,1868,1887,2027,2029,2031,2058,2062,2064,2066,2070,2072,2082,2086,2089,2197,2203,2206,2209,2220,2226,2229,2232,2234,2237,2241,2243,2247,2248,2250,2253,2254,2258,2259,2288,2297,2417,2424,2425,2427,2429,2432,2433,2434,2441,2443,2445,2449,2451,2466,2594,2595,2597,2602,2605,2770,2773,2775,2781,2782,2784,2789,2795,2807,2808,2811,2814,2960,2970,2974,2975,3120,3131,3141,3146,3147,3150,3155,3159,3162,3164,3168,3170,3173,3179,3185,3196,3330,3333,3337,3340,3341,3346,3360,3379,3683,3691,3694,3697,3702,3709,3711,3726,3729,3731,3733,3735,3738,3739,3741,3743,3747,3756,3758,3762,3764,3782,3785,3787,3790,3795,3806,3937,3942,3945,3947,3953,3955,3956,3959,3960,3964,3966,3969,3973,3975,3977,3978,3982,3985,3991,3993,3996,4001,4158,4160,4164,4167,4170,4181,4183,4186,4191,4195,4198,4201,4357,4359,4360,4362,4365,4366,4368,4369,4371,4372,4374,4378,4380,4526,4527,4530,4532,4535,4538,4559,4691,4695,4697,4698,4706,4709,4712,4719,4723,4736,4738,4740,4745,4751,4754,4756,4757,4760,4764,4765,4769,4771,4773,4775,4777,4784,4904,4907,4913,4922,4925,4928,4934,4937,4938,4941,4944,4947,4948,4955,4960,4962,4963,4965,4967,4969,4971,4973,4976,4978,4979,4982,4985,4986,4988,4996,5000,5001,5005,5008,5013,5016,5042,5134,5136,5140,5143,5146,5148,5149,5154,5157,5158,5160,5162,5163,5164,5166,5169,5180,5181,5184,5210,5347,5348,5349,5351,5353,5354,5357,5362,5370,5373,5375,5379,5386,5389,5390,5392,5397,5400,5403,5406,5407,5412,5415,5417,5419,5565,5566,5569,5574,5576,5584,5586,5588,5590,5593,5595,5598,5600,5602,5604,5609,5610,5615,5622,5623,5647,5769,5780,5783,5786,5788,5791,5800,5808,5811,5813,5816,5818,5822,5830,5852,5856,5858,5863,5865,5868,5984,5987,5994,6001,6007,6011,6013,6017,6022,6024,6028,6031,6035,6036,6039,6040,6170,6183,6189,6197,6213,6217,6219,6225,6233,6234,6236,6240,6241,6242,6248,6251,6253,6255,6257,6258,6262,6266,6283,6319,6414,6416,6417,6421,6428,6433,6435,6436,6439,6440,6442,6443,6445,6446,6449,6450,6453,6459,6463,6471,6576,6587,6602,6607,6610,6613,6614,6617,6619,6620,6639,6642,6648,6653,6814,6817,6818,6843,6845,6989,6991,6994,6999,7004,7009,7012,7014,7016,7018,7023,7186,7190,7194,7208,7213,7215,7217,7219,7363,7370,7373,7381,7388,7405,7419,7567,7570,7573,7574,7577,7580,7594,7597,7603,7604,7608,7610,7613,7617,7623,7628,7767,7777,7780,7793,7798,7966,7979,7984,8142,8147,8148,8150,8152,8156,8158,8166,8175,8180,8312,8314,8324,8329,8336,8339,8340,8343,8345,8348,8515,8521,8523,8534,8536,8538,8543,8549,8551,8553,8690,8715,8719,8727,8731,8743,8877,8883,8889,8908,8913,8915,9076,9090,9132,9134,9135,9272,9275,9277,9280,9443,9447,9618,9748,9755,9762,9766,9772,9774,9779,9783,9789,9791,9792,9794,9797,9798,9799,9804,9808,9813,9816,9817,9821,9822,9824,9825,9828,9829,9831,9834,9843,9850,9851,9852,9854,9858,9861,9864,9866,9867,9870,9872,9875,9881,9882,9884,9886,9888,9890,9893,9894,9895,9901,9902,9905,9906,9909,9911,9913,10022,10031,10032,10034,10037,10045,10050,10051,10053,10067,10071,10073,10083,10089,10102,10189,10208,10213,10217,10220,10223,10228,10232,10234,10362,10381,10546,10549,10553,10558,10566,10568,10571,10574,10576,10589,10594,10596,10599,10600,10602,10603,10740,10741,10742,10743,10747,10749,10751,10755,10756,10759,10764,10768,10771,10774,10789,10791,10795,10805,10808,10818,10829,10859,10907,10924,10927,10929,10934,10935,10938,10940,10943,10945,10948,10950,10951,10954,10956,10959,10963,10972,10975,10985,10987,11097,11107,11108,11111,11115,11117,11120,11122,11125,11129,11133,11135,11137,11139,11143,11145,11147,11149,11150,11152,11178,11180,11183,11184,11186,11189,11209,11210,11328,11338,11341,11349,11350,11353,11356,11358,11359,11362,11373,11375,11378,11379,11388,11389,11395,11397,11400,11402,11403,11404,11406,11408,11411,11417,11512,11523,11528,11548,11551,11553,11554,11559,11561,11566,11568,11571,11580,11583,11588,11589,11592,11594,11596,11601,11602,11607,11617,11620,11625,11627,11631,11633,11638,11645,11652,11784,11786,11794,11796,11797,11800,11801,11803,11806,11808,11830,11833,11839,11843,11969,11975,11977,11979,11983,11984,11988,11991,12004,12014,12019,12020,12021,12023,12027,12036,12039,12042,12049,12050,12054,12056,12060,12066,12197,12199,12200,12202,12205,12208,12211,12371,12374,12386,12388,12393,12396,12398,12462,12563,12565,12566,12571,12572,12575,12579,12584,12586,12589,12591,12596,12600,12610,12613,12704,12707,12736,12737,12739,12741,12745,12750,12753,12756,12758,12759,12764,12765,12766,12770,12772,12773,12793,12812,12931,12937,12953,12962,12963,12966,12967,12969,12981,13132,13135,13148,13151,13153,13162,13317,13332,13334,13336,13339,13342,13344,13347,13354,13500,13513,13517,13519,13525,13526,13529,13534,13536,13537,13540,13542,13543,13544,13547,13548,13551,13552,13554,13558,13577,13737,13738,13742,13746,13750,13752,13753,13757,13758,13761,13764,13899,13914,13915,13916,13917,13921,13925,13961,14072,14085,14089,14090,14095,14096,14099,14104,14105,14113,14119,14121,14124,14125,14132,14133,14134,14137,14140,14142,14165,14246,14272,14276,14277,14280,14282,14290,14295,14298,14306,14308,14314,14317,14320,14324,14327,14330,14331,14333,14337,14339,14345,14348,14354,14356,14358,14361,14362,14485,14487,14488,14493,14495,14501,14503,14504,14507,14509,14511,14513,14517,14527,14530,14534,14667,14668,14670,14671,14676,14679,14680,14682,14686,14690,14691,14696,14697,14701,14744,14885,14887,14890,14891,15054,15058,15062,15064,15065,15067,15070,15072,15082,15089,15106,15139,15257,15259,15262,15263,15278,15281,15291,15292,15294,15296,15308,15311,15428,15444,15455,15458,15460,15468,15473,15477,15479,15488,15490,15493,15494,15496,15498,15499,15500,15502,15504,15505,15509,15516,15517,15520,15523,15525,15527,15529,15533,15535,15540,15688,15689,15694,15701,15703,15709,15710,15711,15713,15715,15717,15725,15729,15731,15733,15755,15758,15897,15903,15904,15906,15907,15911,15912,15914,15915,15919,15926,15942,15944,15950,16076,16120,16126,16129,16132,16135,16138,16140,16143,16145,16147,16153,16155,16156,16159,16163,16165,16167,16170,16284,16288,16291,16294,16297,16301,16303,16309,16313,16317,16331,16333,16335,16337,16342,16352,16356,16503,16512,16519,16521,16525,16527,16544,16545,16683,16686,16698,16699,16703,16707,16709,16710,16712,16713,16716,16717,16718,16720,16722,16724,16725,16728,16730,16732,16735,16737,16739,16743,16745,16749,16751,16752,16755,16759,16773,16781,16790,16909,16919,16922,16924,16928,16934,16936,16938,16940,16942,16944,16946,16950,16952,16953,16956,16957,16959,16960,16962,16963,16964,16973,16974,16977,16979,16980,16988,16990,17002,17004,17094,17110,17115,17116,17119,17122,17126,17128,17132,17136,17137,17139,17140,17143,17149,17150,17152,17155,17160,17163,17166,17168,17185,17300,17306,17316,17318,17320,17326,17327,17330,17335,17337,17342,17344,17346,17348,17351,17353,17356,17359,17360,17363,17368,17370,17371,17374,17376,17377,17378,17380,17389,17393,17395,17396,17401,17407,17410,17412,17416,17418,17420,17423,17426,17433,17439,17441,17442,17446,17582,17612,17614,17617,17619,17631,17632,17633,17636,17638,17640,17644,17646,17649,17779,17805,17808,17810,17812,17814,17816,17818,17819,17846,17994,17997,17999,18002,18174,18177,18188,18192,18212,18215,18217,18355,18397,18400,18401,18404,18405,18407,18415,18568,18577,18580,18589,18591,18624,18720,18736,18739,18743,18745,18749,18750,18752,18755,18756,18758,18762,18764,18765,18766,18769,18770,18772,18773,18776,18788,18791,18793,18795,18797,18802,18809,18816,18826,18951,18953,18954,18958,18969,18971,18974,18976,18988,18989,18994,18999,19002,19004,19008,19010,19016,19164,19167,19172,19173,19175,19178,19180,19184,19200,19201,19202,19205,19206,19210,19223,19226,19229,19230,19373,19375,19381,19384,19390,19391,19395,19396,19398,19400,19402,19406,19410,19412,19414,19417,19427,19429,19431,19432,19435,19437,19441,19444,19587,19601,19606,19608,19609,19610,19626,19717,19766,19767,19769,19772,19798,19799,19802,19817,19818,19820,19824,19827,19975,19979,19980,20158,20195,20320,20325,20337,20344,20349,20353,20372,20374,20383,20390,20393,20395,20396,20399,20405,20424,20558,20572,20577,20578,20580,20582,20583,20584,20585,20587,20588,20594,20595,20602,20608,20755,20772,20778,20782,20783,20785,20787,20790,20793,20804,20805,20808,20811,20813,20815,20816,20837,20978,20980,20982,20987,20989,21002,21005,21006,21008,21012,21014,21016,21018,21020,21025,21030,21032,21034,21037,21039,21043,21049,21056,21203,21206,21209,21211,21212,21379,21383,21389,21391,21395,21398,21401,21405,21412,21413,21416,21419,21420,21421,21426,21427,21431,21435,21436,21438,21440,21442,21449,21451,21454,21459,21464,21469,21471,21475,21477,21478,21481,21482,21483,21494,21560,21609,21611,21613,21615,21621,21627,21630,21633,21636,21655,21656,21658,21807,21809,21810,21813,21816,21819,21821,21997,21999,22021,22026,22031,22033,22061,22202,22204,22206,22210,22213,22217,22221,22223,22381,22387,22391,22393,22394,22403,22404,22551,22576,22578,22583,22587,22589,22594,22596,22597,22598,22605,22608, ptg000001l:13560-36197:m54329U_210323_190418/32113767/ccs block 128,0,128,0 Ignored m6A +ptg000001l 13655 36154 5mC 0 - 13655 36154 139,69,19,1 120 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,34,37,786,997,1020,1407,1419,1517,1570,1636,1966,1986,2053,2075,2212,4315,4599,4692,4704,4741,4749,4754,4776,4784,4800,4802,4822,4828,4845,4865,5323,5760,5762,6576,6653,6754,6805,6807,7281,7675,8066,8239,8327,8329,9529,9723,9753,9790,9915,9971,9975,9977,9998,10007,10011,10113,10161,10169,10223,10231,10239,10792,10795,10798,10807,10835,10844,10865,10896,10937,10941,10961,10985,10992,11000,11482,11744,12107,12517,12655,12931,12939,12947,12971,12977,13033,13053,13057,13099,13110,13144,13469,13711,14215,14224,14526,15295,15561,15568,16297,16791,16946,17113,17133,17574,18286,18465,19010,19020,19537,19722,19724,19808,21650,21809,21819,22014,22433,22498, ptg000001l:13560-36197:m54329U_210323_190418/32113767/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 13750 36118 NUC 0 - 13750 36118 169,169,169,1 113 169,139,115,117,135,130,111,109,84,136,139,107,119,127,164,145,144,133,303,130,156,155,145,131,119,117,136,145,121,115,129,130,104,160,143,162,143,147,138,167,157,131,166,136,133,160,136,162,170,129,108,86,127,164,136,109,117,94,131,125,130,159,164,90,118,150,154,145,159,134,146,106,122,132,140,162,150,116,147,138,169,113,146,137,118,89,114,135,129,147,171,137,152,128,124,147,142,142,139,147,177,161,133,146,140,146,166,114,148,175,140,157,146, 0,170,373,569,773,921,1145,1308,1418,1545,1705,1907,2115,2284,2423,2632,2793,3014,3197,3624,3819,4019,4198,4377,4602,4834,5028,5237,5465,5686,5858,6101,6289,6471,6663,6841,7037,7237,7446,7616,7802,7998,8166,8371,8561,8733,8953,9098,9265,9436,9731,9920,10052,10199,10421,10805,11028,11235,11470,11661,11884,12029,12216,12431,12630,12799,12980,13172,13395,13582,13743,13983,14180,14352,14562,14709,14924,15129,15358,15576,15768,15988,16174,16363,16608,16822,17003,17264,17467,17664,17820,18035,18233,18409,18644,18834,19048,19262,19444,19645,19798,19976,20242,20426,20655,20874,21030,21312,21476,21639,21879,22041,22222, ptg000001l:13560-36197:m54329U_210323_190418/32113767/ccs block 169,169,169,0 Ignored NUC +ptg000001l 23315 23481 FIRE 0 - 23315 23481 175,0,0,1 1 166, 0, ptg000001l:13560-36197:m54329U_210323_190418/32113767/ccs block 175,0,0,0 Ignored FIRE +ptg000001l 30867 31014 FIRE 0 - 30867 31014 225,0,0,1 1 147, 0, ptg000001l:13560-36197:m54329U_210323_190418/32113767/ccs block 225,0,0,0 Ignored FIRE +ptg000001l 13725 35972 LINKER 0 - 13725 35972 147,112,219,1 111 25,1,64,81,87,13,94,52,1,43,24,63,101,50,12,45,16,77,50,124,65,44,24,34,94,113,77,73,83,100,57,114,58,78,32,35,34,57,62,32,19,39,37,39,54,39,60,9,5,1,81,46,20,58,248,114,90,141,60,98,15,28,51,109,51,31,38,78,28,27,94,91,50,78,7,53,55,113,71,54,51,73,43,108,96,92,68,68,9,44,61,24,107,66,67,72,40,62,6,1,105,51,83,79,10,116,50,15,65,22,24, 0,194,334,513,711,933,1076,1281,1442,1527,1706,1869,2039,2259,2436,2612,2802,2962,3172,3525,3779,4000,4199,4368,4533,4746,4976,5189,5407,5611,5826,6012,6256,6418,6656,6831,7028,7205,7409,7609,7808,7984,8154,8357,8532,8719,8918,9114,9285,9460,9864,10031,10204,10388,10582,10939,11170,11354,11626,11811,12039,12213,12405,12546,12773,12974,13159,13342,13579,13741,13914,14114,14327,14509,14727,14896,15099,15270,15530,15739,15962,16126,16345,16525,16751,16936,17424,17621,17836,18016,18197,18410,18562,18793,19006,19215,19429,19608,19817,20000,20162,20400,20597,20820,21045,21221,21451,21649,21839,22044,22223, ptg000001l:13560-36197:m54329U_210323_190418/32113767/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 14289 40504 m6A 0 + 14289 40504 128,0,128,1 1076 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,96,102,438,439,444,454,456,461,466,470,477,478,480,483,485,487,494,499,512,515,518,651,657,658,662,664,667,670,672,673,696,787,826,840,842,844,846,851,854,856,859,1016,1031,1035,1037,1046,1225,1226,1231,1278,1375,1388,1391,1392,1394,1396,1397,1543,1566,1569,1571,1575,1581,1583,1586,1588,1591,1595,1599,1601,1603,1604,1605,1609,1678,1681,1763,1766,1767,1771,1772,2125,2291,2428,2448,2457,2590,2596,2599,2608,2609,2611,2615,2618,2619,2624,2685,3034,3142,3144,3148,3149,3151,3152,3164,3225,3227,3320,3337,3342,3363,3503,3519,3526,3703,3704,3707,3715,3778,3866,3867,3869,3873,3874,3877,3879,3882,3923,4014,4018,4023,4034,4053,4055,4062,4071,4075,4082,4085,4088,4090,4238,4240,4247,4251,4381,4388,4391,4396,4412,4414,4418,4421,4577,4581,4600,4604,4605,4608,4627,4667,4668,4754,4760,4778,4788,4792,4794,4797,4801,4802,4805,4816,4822,4900,5001,5003,5004,5008,5161,5177,5180,5181,5185,5334,5350,5353,5356,5360,5362,5367,5374,5385,5391,5520,5522,5523,5526,5529,5536,5541,5544,5550,5553,5554,5555,5557,5564,5694,5699,5711,5718,5720,5731,5739,5741,5743,5746,5749,5914,5917,5920,6084,6095,6111,6121,6123,6125,6252,6257,6259,6262,6267,6269,6272,6276,6282,6287,6290,6299,6466,6468,6472,6480,6483,6486,6491,6630,6815,6830,6835,6838,6841,6844,6846,6961,6963,7025,7036,7042,7276,7278,7316,7322,7328,7465,7468,7473,7475,7477,7481,7484,7490,7493,7497,7509,7512,7626,7653,7663,7664,7665,7667,7670,7674,7827,7829,7831,7838,8021,8025,8201,8207,8319,8573,8576,8660,8725,8727,8775,8781,8866,8874,8876,8880,8883,8887,8888,8891,8892,8897,8899,8903,8915,8921,8923,8929,9052,9063,9067,9069,9070,9072,9075,9080,9082,9091,9139,9254,9257,9315,9408,9417,9422,9424,9430,9432,9584,9594,9600,9637,9658,9672,9782,9785,9920,9923,9928,9933,9958,9963,10150,10152,10322,10325,10340,10343,10345,10346,10377,10515,10519,10521,10529,10533,10535,10545,10548,10550,10552,10718,10721,10724,10730,10735,10747,10752,10885,10887,10898,10905,10906,10909,10911,10916,10923,10927,10930,10932,10935,10937,10939,10941,10945,10958,10963,11086,11092,11096,11099,11101,11103,11108,11111,11117,11121,11123,11126,11128,11130,11132,11133,11135,11136,11139,11140,11142,11143,11147,11154,11158,11164,11262,11292,11297,11313,11314,11317,11328,11332,11334,11375,11378,11469,11474,11475,11477,11478,11480,11482,11483,11486,11489,11493,11652,11664,11671,11674,11828,11830,11832,11838,11841,11853,11855,11856,11857,11859,11860,11862,11870,11874,11888,11891,11894,11896,12037,12039,12040,12205,12209,12215,12222,12233,12293,12378,12382,12385,12389,12400,12538,12541,12561,12722,12725,12727,12730,12734,12736,12739,12741,12744,12748,12760,12763,12768,12858,12885,12887,12904,12915,12917,12948,12954,13102,13117,13118,13120,13124,13259,13260,13264,13266,13268,13271,13272,13275,13443,13464,13465,13470,13472,13475,13477,13480,13483,13486,13496,13626,13632,13636,13649,13664,13667,13672,13793,13795,13808,13812,13817,13828,13831,13835,13837,13849,13852,13921,13980,13984,13992,13996,14022,14251,14256,14261,14292,14295,14303,14305,14308,14311,14312,14316,14331,14332,14336,14342,14471,14474,14477,14478,14481,14498,14501,14503,14504,14506,14640,14644,14650,14653,14751,14811,14813,14985,14987,14988,14991,14993,14997,15007,15175,15177,15190,15193,15198,15352,15354,15361,15363,15520,15529,15533,15538,15541,15544,15549,15551,15556,15559,15587,15688,15703,15714,15716,15717,15721,15723,15725,15731,15732,15736,15738,15739,15748,15749,15751,15756,15759,15760,15762,15919,15920,15921,15923,15925,15933,15938,15941,15945,15947,15961,15976,16128,16130,16140,16145,16245,16308,16310,16314,16472,16503,16506,16508,16511,16528,16590,16641,16701,16704,16707,16711,16716,16719,16892,16895,16897,16909,16974,17043,17069,17205,17209,17211,17215,17216,17221,17227,17229,17231,17238,17241,17246,17395,17396,17399,17427,17455,17457,17459,17565,17568,17570,17593,17599,17608,17614,17717,17719,17722,17773,17788,17791,17793,17798,17801,17944,17948,17953,17955,17960,17968,17969,17970,17971,17973,17977,18039,18133,18134,18160,18193,18197,18236,18239,18336,18338,18346,18349,18352,18354,18356,18359,18367,18369,18370,18373,18381,18387,18389,18401,18404,18407,18458,18547,18549,18738,18743,18744,18748,18751,18757,18762,18764,18914,18919,18920,18925,18929,18932,18933,18938,18944,18947,18974,19032,19035,19105,19117,19274,19276,19277,19378,19440,19452,19463,19468,19473,19603,19642,19650,19657,19661,19662,19668,19673,19674,19677,19787,19993,19996,19998,20003,20005,20007,20010,20017,20022,20024,20026,20028,20030,20031,20158,20164,20188,20189,20200,20203,20348,20350,20376,20496,20497,20499,20502,20673,20677,20678,20679,20681,20683,20689,20691,20694,20711,20713,20780,20841,20845,20856,20858,20861,20870,20873,20875,20877,20882,20885,20889,20891,20893,21031,21035,21037,21042,21044,21046,21050,21051,21054,21056,21061,21063,21068,21070,21075,21208,21214,21218,21221,21224,21227,21230,21233,21238,21244,21246,21249,21250,21253,21400,21403,21408,21412,21413,21418,21422,21577,21589,21610,21782,21786,21836,21950,21963,21966,22006,22117,22138,22141,22143,22150,22152,22157,22159,22162,22164,22165,22168,22171,22177,22186,22189,22197,22200,22206,22216,22351,22356,22361,22405,22410,22440,22563,22567,22571,22585,22596,22600,22603,22620,22757,22776,22779,22780,22896,22912,22918,22921,22932,22936,22937,22940,22941,22943,22947,22960,22964,23015,23045,23126,23131,23133,23134,23137,23140,23156,23160,23162,23164,23166,23167,23171,23299,23349,23351,23354,23355,23358,23359,23364,23369,23371,23378,23387,23394,23537,23541,23543,23546,23548,23550,23551,23556,23728,23731,23733,23737,23740,23745,23748,23755,23756,23757,23761,23931,23964,23965,23971,24022,24062,24069,24113,24114,24116,24120,24123,24125,24126,24187,24294,24299,24303,24307,24310,24311,24313,24316,24318,24321,24322,24334,24337,24341,24450,24496,24498,24503,24510,24520,24524,24526,24634,24693,24739,24850,24852,24860,24871,24879,24881,24883,24885,24888,24893,24897,24898,24900,24905,24949,25044,25134,25229,25245,25268,25273,25429,25638,25642,25643,25645,25674,25677,25681,25810,25812,25814,25815,25819,25821,25824,25826,25833,25836,25839,26009,26169,26172,26176,26178,26181,26193,26214, ptg000001l:14275-40558:m54329U_210323_190418/66718332/ccs block 128,0,128,0 Ignored m6A +ptg000001l 14416 40524 5mC 0 + 14416 40524 139,69,19,1 177 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,24,235,629,645,657,1488,2197,2388,2724,2961,3553,3930,3936,3942,3979,4031,4038,4040,4060,4066,4103,4424,4485,4506,4598,4998,5000,5141,5310,5711,5814,7243,7304,7334,7359,7374,7477,7497,7508,7518,7522,7565,7567,7941,8067,8336,8367,8757,8767,8961,8991,9209,9213,9215,9236,9245,9249,9256,9301,9351,9461,9469,9477,10030,10033,10036,10045,10073,10082,10103,10175,10179,10223,10238,10260,10434,11738,11755,11893,11952,12209,12215,12232,12256,12271,12291,12295,12337,12348,12368,12393,12395,12426,12623,12707,12949,12956,14589,14593,14620,14631,14799,14806,15535,15844,15939,16184,16200,16242,16351,17703,17867,18248,18618,18860,18871,18962,19046,19067,20071,20121,21047,21057,21189,21213,21252,21671,21736,22018,22160,22626,22767,22773,22807,22821,22830,22861,22865,22955,23493,23909,24235,24365,24711,24891,25487,25492,25678,25731,25744,25753,25756,25758,25791,25793,25797,25810,25812,25816,25838,25862,25867,25874,25886,25901,25904,25908,25916,25953,25964,25973,25980,25987,26063,26095,26107, ptg000001l:14275-40558:m54329U_210323_190418/66718332/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 14392 40458 NUC 0 + 14392 40458 169,169,169,1 139 335,132,129,156,178,143,145,81,352,165,136,132,348,107,92,139,176,150,131,147,129,155,85,77,100,152,148,128,129,164,163,126,166,138,184,114,233,136,140,152,182,175,111,253,148,84,122,162,150,151,109,134,186,169,137,165,132,122,127,90,158,153,140,164,144,137,160,116,147,134,167,129,120,127,228,128,133,157,171,167,153,156,99,156,151,162,157,172,172,133,135,148,105,102,142,155,96,139,188,149,130,156,162,129,109,205,126,144,145,170,127,137,132,146,154,171,163,150,134,152,136,115,110,127,142,171,169,167,154,166,156,138,89,94,155,208,128,169,159, 0,416,594,757,944,1129,1295,1579,1670,2023,2189,2355,2583,2932,3125,3261,3424,3613,3780,3988,4149,4319,4566,4720,4798,4906,5083,5289,5462,5647,5818,6023,6197,6389,6528,6744,6940,7226,7410,7572,7736,7923,8105,8217,8474,8679,8827,8989,9155,9330,9570,9683,9861,10050,10275,10450,10650,10861,11062,11276,11391,11572,11794,11938,12131,12298,12459,12666,12852,13022,13173,13394,13570,13750,13920,14240,14404,14551,14711,14905,15096,15261,15486,15660,15874,16043,16212,16426,16617,16807,16967,17144,17357,17512,17699,17875,18137,18305,18447,18662,18872,19015,19175,19371,19575,19685,19929,20101,20248,20400,20611,20791,20973,21151,21320,21508,21684,21864,22114,22308,22518,22678,22913,23069,23292,23454,23659,24024,24239,24424,24591,24803,24942,25032,25171,25327,25579,25737,25907, ptg000001l:14275-40558:m54329U_210323_190418/66718332/ccs block 169,169,169,0 Ignored NUC +ptg000001l 38220 38416 FIRE 0 + 38220 38416 255,0,0,1 1 196, 0, ptg000001l:14275-40558:m54329U_210323_190418/66718332/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 14385 40504 LINKER 0 + 14385 40504 147,112,219,1 139 7,81,46,34,31,7,23,139,10,1,1,30,96,1,86,44,24,13,17,77,14,41,92,69,1,8,25,58,45,56,7,42,48,26,1,32,82,53,48,22,12,5,7,1,4,57,64,40,4,25,89,4,44,3,56,38,35,79,79,87,25,23,69,4,29,23,24,47,70,23,17,54,47,60,43,92,36,14,3,23,24,12,68,75,58,18,7,57,19,18,27,42,65,50,85,34,107,72,3,27,61,13,4,34,75,1,39,46,3,7,41,53,45,46,23,34,5,17,100,60,58,24,120,46,96,20,34,48,31,1,56,1,1,45,1,44,30,1,46, 0,342,555,730,920,1129,1279,1447,1667,2029,2195,2332,2494,2938,3046,3224,3407,3607,3770,3918,4142,4285,4481,4658,4804,4905,5065,5238,5424,5598,5818,5988,6156,6370,6534,6719,6865,7180,7369,7557,7731,7925,8105,8223,8477,8629,8770,8956,9158,9312,9488,9686,9824,10054,10226,10419,10622,10789,10990,11196,11373,11556,11732,11941,12109,12282,12442,12626,12789,13006,13163,13347,13530,13697,13884,14155,14375,14544,14715,14889,15079,15256,15424,15592,15823,16032,16212,16376,16605,16796,16947,17109,17299,17469,17621,17848,18037,18240,18451,18642,18818,19009,19178,19344,19507,19691,19897,20062,20252,20400,20577,20745,20935,21112,21304,21481,21686,21854,22021,22255,22467,22661,22800,23030,23203,23441,23632,24198,24400,24597,24754,24948,25038,25133,25333,25542,25714,25913,26073, ptg000001l:14275-40558:m54329U_210323_190418/66718332/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 16121 34715 m6A 0 - 16121 34715 128,0,128,1 1358 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,2,5,7,24,27,40,43,58,173,176,198,203,211,213,216,219,221,222,227,228,230,235,250,254,369,381,385,386,389,396,399,404,406,411,412,414,416,420,446,453,459,596,614,616,619,625,629,631,634,642,645,654,783,786,792,797,990,994,1000,1003,1004,1005,1007,1030,1031,1033,1035,1038,1180,1181,1184,1185,1187,1189,1190,1191,1193,1195,1368,1382,1385,1388,1391,1393,1395,1399,1401,1412,1419,1423,1551,1552,1554,1558,1574,1575,1582,1584,1586,1589,1590,1592,1593,1596,1599,1601,1604,1606,1610,1613,1616,1627,1757,1759,1760,1762,1776,1781,1785,1801,1803,1805,1808,1811,1812,1814,1815,1817,1818,1820,1824,1826,1829,1833,1836,1840,1841,1842,1845,1912,1978,1984,1989,1992,1994,1995,1998,2002,2005,2007,2008,2009,2010,2012,2018,2028,2155,2158,2163,2166,2169,2180,2182,2184,2186,2188,2191,2192,2197,2200,2202,2210,2214,2215,2217,2221,2223,2230,2239,2242,2245,2359,2368,2371,2376,2380,2383,2384,2387,2390,2393,2394,2411,2415,2417,2419,2422,2424,2508,2537,2542,2549,2556,2559,2564,2567,2572,2575,2580,2582,2592,2594,2595,2600,2601,2603,2604,2606,2608,2609,2610,2612,2615,2616,2621,2626,2627,2767,2768,2769,2772,2775,2777,2779,2782,2784,2788,2789,2793,2794,2795,2797,2799,2800,2803,2808,2821,2832,2836,2946,2960,2962,2969,2970,2975,2981,2984,2986,2988,2990,2993,2995,2996,3002,3012,3015,3019,3022,3189,3194,3200,3205,3208,3209,3212,3232,3234,3237,3381,3382,3384,3395,3396,3398,3400,3424,3433,3559,3574,3578,3582,3596,3598,3602,3603,3608,3610,3614,3616,3623,3626,3628,3640,3773,3798,3805,3806,3809,3824,3828,3835,3951,3957,3975,3976,3979,3982,3984,3987,3988,3989,3994,4018,4019,4021,4022,4182,4189,4191,4207,4355,4359,4361,4366,4372,4376,4379,4382,4388,4394,4398,4401,4404,4407,4422,4425,4743,4952,4979,5069,5133,5148,5152,5159,5307,5318,5329,5330,5333,5337,5495,5517,5519,5520,5535,5540,5542,5550,5554,5555,5556,5714,5718,5721,5724,5730,5735,5755,5756,5758,5760,5770,5774,5775,5782,5785,5786,5789,5791,5909,5925,5927,5946,6094,6096,6098,6103,6116,6134,6138,6295,6300,6302,6309,6313,6316,6318,6321,6323,6326,6329,6330,6336,6346,6481,6485,6489,6490,6494,6509,6514,6522,6547,6817,6830,6836,6838,6842,6845,6848,6853,6856,6857,6859,6860,6862,6869,6871,6905,7023,7025,7034,7038,7042,7044,7045,7048,7050,7051,7055,7056,7058,7059,7064,7065,7067,7071,7083,7086,7091,7097,7106,7109,7111,7208,7212,7220,7275,7389,7395,7416,7417,7422,7423,7584,7590,7592,7603,7752,7758,7760,7762,7766,7768,7770,7774,7776,7802,7805,7806,7808,7810,7963,7967,7969,7980,7985,7990,7999,8003,8004,8009,8011,8012,8014,8017,8020,8148,8151,8155,8156,8158,8161,8165,8167,8179,8185,8193,8195,8197,8202,8204,8205,8210,8353,8370,8373,8375,8380,8381,8383,8384,8386,8389,8391,8394,8396,8397,8400,8402,8405,8406,8409,8530,8536,8553,8554,8557,8561,8563,8566,8568,8571,8575,8579,8581,8583,8585,8589,8591,8593,8595,8598,8756,8758,8761,8762,8774,8776,8777,8779,8781,8782,8784,8785,8786,8787,8793,8795,8799,8802,8804,8805,8808,8810,8821,8941,8943,8958,8960,8962,8965,8969,8971,8975,8980,8981,8983,8985,8986,8988,8991,8994,8997,8999,9000,9005,9007,9009,9011,9014,9017,9022,9029,9140,9150,9152,9153,9155,9158,9160,9162,9165,9168,9170,9174,9175,9178,9179,9181,9183,9185,9194,9200,9201,9203,9205,9214,9216,9232,9240,9242,9243,9246,9247,9248,9249,9252,9254,9256,9257,9260,9267,9269,9272,9276,9279,9285,9289,9291,9398,9405,9409,9421,9423,9425,9426,9427,9429,9430,9434,9436,9455,9460,9465,9466,9633,9637,9642,9643,9645,9646,9648,9650,9654,9657,9659,9832,9834,9839,9840,9842,9844,9852,9854,9856,9859,9888,9893,9979,9981,9982,9986,9996,9998,10001,10004,10006,10009,10011,10012,10017,10018,10021,10023,10024,10027,10028,10030,10032,10035,10038,10041,10042,10046,10049,10053,10054,10056,10059,10062,10064,10191,10226,10230,10237,10239,10243,10244,10250,10253,10366,10383,10390,10391,10393,10397,10401,10408,10409,10412,10413,10415,10416,10421,10426,10428,10574,10577,10594,10597,10599,10626,10629,10775,10778,10780,10782,10790,10792,10807,10810,10812,10815,10817,10818,10822,10824,10827,10831,10833,10836,10838,10841,10843,10846,10998,11023,11024,11026,11027,11029,11031,11036,11050,11052,11054,11057,11060,11061,11191,11195,11196,11203,11210,11234,11236,11240,11242,11243,11246,11249,11261,11262,11264,11267,11270,11272,11281,11286,11292,11406,11414,11418,11420,11421,11423,11425,11427,11428,11432,11434,11439,11440,11443,11446,11448,11449,11451,11454,11455,11457,11460,11469,11471,11472,11478,11480,11486,11489,11638,11640,11643,11645,11648,11651,11654,11657,11661,11664,11666,11670,11673,11678,11680,11682,11685,11688,11692,11694,11698,11700,11701,11703,11705,11710,11712,11714,11814,11829,11832,11835,11845,11849,11852,11854,11858,11860,11869,11871,11875,11877,11879,11880,11884,11888,11891,11898,11900,11903,11907,11910,12032,12045,12063,12066,12068,12230,12236,12240,12241,12244,12246,12247,12248,12251,12254,12260,12261,12263,12264,12268,12279,12281,12285,12287,12293,12300,12333,12451,12456,12460,12463,12475,12476,12477,12479,12639,12642,12645,12648,12649,12653,12655,12659,12666,12669,12671,12672,12674,12679,12697,12808,12818,12830,12834,12839,12841,12844,12848,12850,12853,12854,12856,12859,12861,12867,12890,13009,13012,13021,13026,13028,13030,13031,13037,13050,13056,13057,13063,13065,13071,13073,13082,13196,13203,13208,13210,13216,13217,13220,13223,13225,13226,13230,13234,13237,13239,13247,13252,13255,13256,13258,13261,13263,13268,13269,13273,13276,13282,13283,13286,13291,13292,13302,13433,13443,13447,13449,13455,13456,13457,13460,13463,13466,13468,13469,13471,13472,13474,13475,13481,13483,13487,13491,13493,13499,13501,13505,13507,13509,13648,13652,13654,13656,13658,13662,13663,13665,13666,13668,13671,13673,13676,13679,13680,13681,13684,13687,13688,13692,13695,13697,13701,13702,13706,13709,13712,13713,13717,13719,13720,13721,13724,13727,13730,13734,13737,13740,13743,13747,13755,13759,13869,13882,13885,13889,13893,13895,13899,13900,13904,13906,13907,13916,13919,13921,13922,13924,13927,13928,13930,13944,13946,13951,14099,14101,14104,14109,14262,14267,14269,14275,14279,14281,14286,14290,14291,14293,14296,14298,14300,14307,14308,14313,14322,14327,14333,14478,14482,14500,14504,14507,14512,14514,14641,14643,14645,14658,14659,14670,14671,14674,14676,14679,14680,14682,14685,14687,14690,14699,14702,14706,14707,14712,14828,14839,14842,14844,14858,14862,14864,14866,14869,14872,14875,14879,14881,14884,14899,14909,14916,15046,15262,15272,15440,15443,15450,15458,15460,15462,15464,15474,15477,15480,15997,16014,16165,16171,16176,16177,16180,16182,16188,16196,16198,16201,16202,16204,16206,16207,16210,16212,16215,16216,16222,16234,16239,16241,16243,16262,16264,16387,16393,16396,16400,16404,16406,16412,16415,16417,16420,16422,16424,16434,16435,16440,16448,16450,16454,16456,16462,16464,16581,16587,16595,16599,16602,16603,16610,16613,16615,16618,16619,16621,16624,16626,16630,16636,16648,16652,16654,16656,16795,16801,16819,16997,16999,17003,17018,17021,17027,17162,17165,17168,17182,17184,17187,17188,17191,17197,17200,17203,17212,17213,17215,17217,17220,17359,17364,17368,17372,17374,17376,17380,17382,17416,17418,17582,17594,17598,17600,17608,17752,17759,17760,17762,17766,17771,17888,17926,17950,17955,17981,18023,18026,18141,18143,18155,18157,18159,18160,18161,18164,18166,18167,18171,18173,18175,18182,18185,18192,18326,18329,18331,18342,18348,18356,18368,18371,18378,18384,18396,18411,18530,18539,18542,18544,18545,18548,18549,18552,18555,18556,18564,18568,18569,18572,18573,18576,18577,18580,18583,18586,18588,18591,18593, ptg000001l:16029-34725:m54329U_210323_190418/90833781/ccs block 128,0,128,0 Ignored m6A +ptg000001l 16336 33880 5mC 0 - 16336 33880 139,69,19,1 97 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,1634,2011,2014,2017,2023,2060,2103,2112,2119,2121,2141,2147,2184,2289,2408,2505,2566,3079,3081,3124,3391,3792,3895,3972,4126,4994,5300,5324,5440,5455,5558,5589,5646,5648,6448,6838,6848,6865,7206,7294,7317,7326,7330,7337,7382,7480,7542,7550,8112,8114,8117,8126,8154,8163,8184,8280,8304,8311,9426,9836,9886,10033,10250,10258,10266,10290,10296,10372,10376,10429,10463,10474,10476,10507,10523,11534,11543,11845,12701,12887,14110,14265,14463,14893,15605,15948,16301,16329,16650,16856,16908,17041,17043,17127,17148,17543, ptg000001l:16029-34725:m54329U_210323_190418/90833781/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 16180 34651 NUC 0 - 16180 34651 169,169,169,1 90 114,114,136,128,192,141,172,127,129,132,126,113,112,139,109,166,143,125,132,115,159,147,317,208,153,147,157,157,117,147,156,134,269,151,96,168,160,148,152,127,142,120,157,119,110,106,166,172,85,126,112,145,145,151,129,113,148,99,121,161,150,159,110,118,113,130,138,109,147,152,144,126,115,129,215,167,516,150,122,116,138,177,134,138,163,143,154,114,133,118, 0,196,401,596,739,980,1137,1365,1569,1787,1970,2187,2366,2569,2778,2964,3179,3375,3582,3777,3964,4149,4367,4685,4921,5101,5279,5498,5733,5888,6080,6288,6489,6813,7053,7162,7365,7545,7752,7962,8152,8351,8540,8763,8971,9233,9408,9601,9835,10006,10195,10370,10571,10788,11003,11234,11431,11656,11852,12010,12242,12421,12639,12832,13024,13244,13451,13701,13893,14051,14275,14456,14654,14858,14988,15214,15422,15956,16206,16406,16598,16761,16969,17162,17360,17550,17713,17968,18134,18353, ptg000001l:16029-34725:m54329U_210323_190418/90833781/ccs block 169,169,169,0 Ignored NUC +ptg000001l 25261 25413 FIRE 0 - 25261 25413 200,0,0,1 1 152, 0, ptg000001l:16029-34725:m54329U_210323_190418/90833781/ccs block 200,0,0,0 Ignored FIRE +ptg000001l 29769 29881 FIRE 0 - 29769 29881 255,0,0,1 1 112, 0, ptg000001l:16029-34725:m54329U_210323_190418/90833781/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 16121 34533 LINKER 0 - 16121 34533 147,112,219,1 88 59,82,91,59,15,49,16,56,77,89,51,91,66,91,70,77,49,53,82,63,72,26,71,1,28,27,31,62,78,38,45,52,67,55,89,13,35,20,59,58,63,57,69,66,89,69,27,62,86,63,63,56,72,64,102,84,77,97,37,71,29,59,83,74,107,77,83,11,72,37,72,89,1,11,41,18,100,78,76,25,31,59,60,27,20,101,52,86, 0,173,369,596,783,990,1180,1368,1551,1757,1978,2155,2359,2537,2767,2946,3189,3381,3559,3773,3951,4182,4355,4743,4952,5133,5307,5495,5714,5909,6094,6295,6481,6817,7023,7208,7389,7584,7752,7963,8148,8353,8530,8756,8941,9398,9633,9832,9979,10191,10366,10574,10775,10998,11191,11406,11638,11814,12032,12230,12451,12639,12808,13009,13196,13433,13869,14099,14262,14478,14641,14828,15046,15262,15440,15997,16165,16387,16581,16795,16997,17162,17359,17582,17752,17926,18141,18326, ptg000001l:16029-34725:m54329U_210323_190418/90833781/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 21247 39350 m6A 0 + 21247 39350 128,0,128,1 1375 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,7,177,181,185,304,307,309,312,318,319,323,326,330,343,345,347,364,370,493,500,507,508,510,515,517,519,523,526,532,535,539,542,547,551,554,558,656,659,668,669,671,674,676,679,681,682,684,685,707,709,712,716,717,718,719,721,725,726,728,741,854,856,874,878,879,901,902,904,906,908,912,913,915,916,917,919,921,926,1063,1065,1076,1082,1083,1086,1088,1091,1093,1096,1100,1104,1126,1133,1249,1282,1287,1301,1314,1452,1454,1455,1458,1470,1619,1634,1637,1639,1640,1817,1818,1833,1983,2154,2163,2350,2352,2477,2640,2642,2793,2804,2807,2815,2816,2820,2824,2827,2959,2961,2962,2965,3109,3111,3113,3115,3125,3128,3254,3260,3265,3270,3271,3274,3276,3279,3280,3283,3287,3292,3295,3305,3307,3431,3437,3440,3442,3445,3449,3453,3455,3457,3459,3463,3467,3469,3470,3472,3496,3601,3626,3630,3635,3644,3645,3648,3650,3651,3652,3655,3658,3661,3662,3666,3667,3669,3672,3673,3676,3678,3679,3682,3684,3695,3698,3699,3709,3715,3817,3832,3834,3836,3839,3843,3845,3848,3849,3854,3855,3857,3859,3860,3862,3865,3868,3881,3883,3891,3990,3997,4000,4004,4005,4012,4014,4018,4020,4022,4024,4026,4027,4029,4032,4034,4036,4039,4041,4042,4044,4046,4048,4049,4052,4053,4059,4060,4063,4065,4066,4068,4070,4071,4074,4075,4077,4215,4220,4223,4240,4244,4246,4265,4272,4279,4283,4424,4426,4442,4445,4471,4590,4629,4630,4633,4635,4636,4640,4645,4805,4807,4809,4834,4838,4841,4842,4843,4845,4847,4849,4850,4853,4855,4856,4860,4865,4990,4992,4995,4997,5005,5008,5015,5017,5022,5024,5025,5027,5033,5038,5042,5044,5052,5057,5151,5153,5157,5163,5166,5168,5173,5176,5177,5180,5183,5185,5189,5191,5193,5195,5196,5201,5202,5203,5205,5206,5210,5211,5213,5219,5221,5232,5235,5236,5238,5251,5257,5380,5390,5395,5396,5399,5405,5408,5412,5413,5415,5416,5420,5421,5424,5427,5431,5433,5437,5442,5444,5445,5448,5451,5452,5454,5468,5471,5599,5616,5619,5632,5638,5643,5645,5650,5654,5656,5659,5661,5664,5786,5795,5800,5802,5805,5810,5814,5816,5820,5821,5823,5824,5831,5833,5834,5837,5839,5840,5842,5845,5846,5993,5996,5998,6002,6004,6007,6009,6012,6013,6014,6016,6019,6021,6024,6141,6144,6154,6160,6162,6166,6175,6180,6185,6188,6189,6193,6195,6199,6203,6205,6211,6213,6214,6216,6218,6219,6228,6234,6240,6331,6363,6368,6371,6374,6376,6380,6383,6384,6385,6399,6400,6405,6409,6416,6419,6424,6547,6552,6554,6556,6559,6562,6566,6568,6572,6574,6575,6577,6579,6580,6583,6584,6586,6588,6592,6596,6597,6600,6602,6610,6611,6615,6618,6626,6628,6743,6745,6762,6765,6768,6772,6774,6777,6781,6783,6784,6787,6789,6790,6802,6805,6807,6808,6811,6813,6815,6817,6821,6823,6827,6833,6835,6837,6839,6844,6902,6907,6929,6937,6940,6942,6943,6944,6947,6948,6961,6963,6965,6967,6970,6973,6974,6976,6978,6979,6991,6996,6999,7000,7006,7128,7142,7153,7155,7159,7161,7164,7167,7174,7179,7180,7185,7187,7188,7190,7193,7316,7319,7322,7324,7330,7334,7337,7338,7343,7345,7347,7350,7353,7358,7361,7362,7364,7367,7368,7371,7372,7373,7374,7377,7378,7382,7384,7385,7387,7389,7390,7392,7513,7516,7519,7520,7522,7523,7527,7529,7533,7538,7540,7543,7545,7546,7548,7553,7554,7558,7559,7565,7571,7682,7690,7692,7695,7696,7704,7708,7713,7718,7722,7724,7727,7728,7730,7733,7739,7853,7855,7867,7868,7870,7876,7881,7883,7885,7886,7888,7891,8033,8071,8074,8075,8077,8078,8080,8082,8087,8090,8091,8095,8097,8111,8226,8230,8231,8234,8239,8247,8252,8262,8264,8268,8269,8270,8274,8277,8410,8416,8429,8431,8440,8445,8446,8449,8452,8455,8569,8583,8586,8591,8593,8598,8601,8604,8605,8608,8611,8614,8617,8621,8623,8629,8633,8637,8639,8640,8644,8651,8741,8743,8756,8763,8765,8773,8778,8790,8791,8793,8798,8801,8802,8804,8818,8820,8821,8823,8825,8827,8968,8970,8971,8973,8975,8976,8978,8984,8987,8989,8992,8995,8996,8998,9001,9003,9019,9023,9027,9145,9149,9155,9158,9166,9167,9170,9172,9174,9177,9178,9179,9181,9182,9185,9186,9187,9194,9196,9201,9205,9207,9210,9212,9227,9229,9350,9352,9356,9358,9360,9361,9364,9366,9368,9374,9378,9381,9386,9392,9395,9515,9517,9519,9544,9545,9548,9550,9553,9554,9556,9561,9564,9570,9576,9702,9706,9709,9713,9858,9862,9865,9870,9872,9876,9879,9880,9883,9884,9886,9889,9891,9894,9895,9902,9915,9917,9918,9920,9922,10064,10076,10079,10212,10214,10216,10217,10218,10221,10232,10236,10237,10238,10241,10247,10248,10251,10253,10256,10263,10269,10271,10273,10414,10416,10422,10425,10436,10438,10441,10442,10586,10604,10607,10610,10612,10618,10653,10790,10793,10925,10927,10929,10937,10944,10945,10967,10969,10970,10972,10979,10981,10990,10995,11102,11108,11111,11115,11117,11129,11130,11136,11143,11145,11146,11150,11152,11153,11159,11165,11166,11170,11322,11324,11328,11333,11335,11336,11338,11341,11343,11361,11487,11657,11661,11663,11664,11666,11669,11670,11675,11798,11804,11827,11829,11830,11837,11842,11964,11966,11967,11969,11971,11974,11975,11980,11985,11986,11989,11993,12118,12122,12126,12140,12144,12147,12150,12155,12159,12288,12292,12303,12306,12308,12309,12313,12316,12319,12320,12324,12467,12468,12474,12477,12478,12481,12482,12484,12485,12488,12505,12645,12651,12652,12653,12657,12664,12669,12671,12673,12800,12802,12817,12818,12825,12829,12843,13015,13016,13017,13020,13040,13044,13045,13157,13177,13179,13185,13199,13200,13203,13338,13340,13345,13350,13352,13355,13357,13359,13361,13367,13369,13382,13388,13520,13523,13524,13526,13529,13533,13695,13699,13703,13720,13721,13723,13725,13730,13731,13739,13741,13872,13874,13891,13896,13917,13919,14071,14077,14079,14081,14084,14086,14088,14092,14093,14096,14108,14117,14239,14242,14246,14254,14256,14260,14263,14266,14267,14269,14272,14275,14277,14280,14282,14283,14286,14288,14291,14292,14295,14307,14418,14424,14427,14431,14435,14440,14442,14443,14446,14450,14452,14455,14457,14460,14464,14474,14477,14478,14480,14486,14488,14618,14619,14623,14625,14628,14630,14633,14652,14791,14800,14802,14804,14807,14814,14826,14829,14833,14834,14867,14972,14983,14992,14994,14996,14998,15008,15013,15123,15133,15135,15137,15139,15140,15146,15150,15153,15155,15158,15159,15160,15163,15167,15168,15170,15175,15177,15180,15182,15183,15185,15191,15194,15198,15210,15213,15217,15312,15318,15326,15331,15332,15334,15335,15336,15339,15341,15342,15345,15349,15354,15358,15362,15364,15366,15369,15499,15502,15503,15505,15506,15510,15514,15516,15518,15520,15526,15528,15532,15562,15695,15700,15701,15705,15711,15713,15714,15717,15734,15762,16003,16006,16010,16012,16013,16015,16019,16022,16026,16029,16048,16173,16175,16176,16182,16200,16202,16204,16206,16208,16209,16211,16213,16226,16228,16229,16231,16234,16236,16390,16393,16396,16397,16401,16403,16404,16407,16414,16420,16421,16423,16424,16426,16428,16429,16431,16443,16561,16564,16566,16570,16571,16575,16579,16585,16588,16590,16592,16593,16598,16599,16600,16603,16609,16613,16769,16773,16775,16776,16777,16782,16785,16786,16787,16790,16792,16796,16797,16799,16941,16943,16947,16956,16959,16962,16964,16967,16973,16978,17138,17140,17141,17150,17155,17156,17158,17161,17162,17164,17165,17167,17168,17170,17171,17173,17174,17175,17181,17187,17189,17194,17297,17302,17305,17311,17313,17319,17321,17325,17328,17342,17345,17349,17353,17355,17358,17360,17363,17482,17487,17495,17503,17506,17510,17512,17516,17519,17523,17526,17531,17538,17540,17545,17552,17662,17663,17664,17668,17674,17676,17680,17683,17690,17693,17695,17697,17699,17701,17706,17834,17861,17865,17868,17870,17872,17877,17902,17904,17913,18032,18034,18037,18038,18048,18053,18056,18058,18059,18063,18066,18072,18073,18079,18080,18082,18085,18086,18087,18089,18095,18097,18102, ptg000001l:21207-39362:m54329U_210323_190418/114362450/ccs block 128,0,128,0 Ignored m6A +ptg000001l 21329 39308 5mC 0 + 21329 39308 139,69,19,1 112 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,330,421,429,461,553,584,595,605,609,652,654,1028,1154,1454,1844,1854,2078,2212,2296,2300,2302,2323,2332,2336,2343,2388,2430,2438,2486,2494,2524,2526,2548,2556,3117,3120,3123,3132,3160,3169,3190,3221,3262,3266,3286,3310,3317,3325,4069,4825,4842,4892,5039,5256,5272,5319,5378,5424,5435,5455,5480,5482,5513,5640,5794,6036,6043,6114,6549,6578,7680,7707,7893,8622,9116,9287,9458,9469,9547,9899,10611,10790,11345,12047,12049,12133,12491,12836,13975,14134,14144,14222,14339,14758,15083,15105,15309,15854,15894,15917,15948,15952,16026,16042,16070,16439,16490,16996,17482,17953,17978, ptg000001l:21207-39362:m54329U_210323_190418/114362450/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 21255 39279 NUC 0 + 21255 39279 169,169,169,1 98 169,118,122,97,112,136,148,137,148,176,149,170,186,124,162,150,131,143,125,123,104,101,98,137,140,144,159,124,93,122,127,121,146,116,122,122,114,121,122,120,110,113,141,114,132,113,89,140,117,120,119,125,144,141,132,140,143,136,131,106,151,125,169,122,121,124,128,142,139,126,171,111,134,131,161,130,151,121,110,129,138,137,109,94,129,132,240,124,153,117,155,141,159,102,118,109,127,118, 0,178,363,551,734,919,1126,1307,1463,1633,1826,1976,2156,2345,2470,2635,2820,2958,3121,3300,3489,3708,3884,4070,4276,4438,4638,4858,5050,5250,5464,5657,5839,6017,6233,6417,6621,6999,7186,7385,7564,7732,7884,8104,8270,8448,8644,8820,9020,9222,9388,9569,9706,9915,10072,10266,10435,10646,10786,10988,11163,11354,11480,11668,11835,11986,12152,12317,12498,12666,12836,13038,13196,13381,13526,13734,13912,14110,14300,14481,14645,14827,15006,15210,15362,15555,15755,16041,16229,16436,16606,16792,16971,17187,17356,17545,17699,17906, ptg000001l:21207-39362:m54329U_210323_190418/114362450/ccs block 169,169,169,0 Ignored NUC +ptg000001l 21424 39161 LINKER 0 + 21424 39161 147,112,219,1 97 9,67,66,86,73,71,33,19,22,17,1,10,3,1,3,35,7,20,54,66,115,75,88,69,22,56,61,68,107,92,66,61,32,100,62,82,264,66,77,59,58,39,79,52,46,83,87,60,85,46,62,12,65,16,62,29,68,4,71,69,40,1,19,45,30,42,37,39,29,44,31,47,51,14,47,48,47,69,71,35,44,42,95,58,64,68,46,64,54,53,31,38,57,67,71,45,80, 0,127,316,479,677,886,1105,1275,1442,1640,1806,1977,2173,2300,2463,2616,2782,2932,3077,3254,3424,3640,3813,4038,4247,4413,4628,4813,4974,5203,5422,5609,5816,5964,6186,6370,6566,6951,7139,7336,7505,7676,7856,8049,8233,8392,8564,8791,8968,9173,9338,9525,9681,9887,10035,10237,10409,10613,10748,10925,11145,11310,11480,11621,11787,11941,12111,12290,12468,12623,12838,12980,13161,13343,13518,13695,13894,14062,14241,14441,14614,14795,14946,15135,15322,15518,15826,15996,16213,16384,16592,16764,16961,17120,17305,17485,17657, ptg000001l:21207-39362:m54329U_210323_190418/114362450/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 23761 44720 m6A 0 + 23761 44720 128,0,128,1 2021 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,3,11,160,162,165,166,168,170,172,173,181,202,219,352,363,364,369,372,374,375,377,380,386,387,405,406,424,545,553,555,557,558,560,562,565,570,577,588,591,594,595,597,598,603,730,733,735,740,743,744,746,749,751,754,756,757,760,762,765,766,903,907,921,928,931,935,941,943,945,949,951,953,955,956,958,984,986,989,990,992,995,1008,1162,1181,1184,1185,1188,1189,1195,1201,1206,1318,1320,1322,1325,1329,1331,1334,1340,1341,1343,1345,1346,1348,1351,1354,1365,1367,1369,1371,1372,1374,1377,1381,1382,1387,1389,1390,1395,1398,1399,1400,1402,1407,1408,1409,1410,1413,1426,1433,1439,1574,1576,1596,1597,1598,1599,1600,1602,1603,1606,1607,1608,1609,1614,1617,1620,1624,1627,1629,1630,1632,1636,1639,1645,1649,1660,1667,1701,1777,1781,1783,1789,1790,1810,1816,1829,1834,1836,1845,1852,1855,1856,1858,1860,1862,1999,2002,2003,2005,2006,2008,2010,2011,2014,2017,2019,2021,2023,2199,2202,2204,2208,2213,2214,2216,2219,2222,2327,2333,2339,2341,2342,2346,2356,2358,2360,2361,2362,2364,2365,2366,2369,2371,2372,2375,2377,2378,2381,2383,2384,2385,2387,2388,2390,2392,2395,2397,2399,2401,2402,2406,2409,2413,2414,2416,2418,2419,2422,2424,2427,2431,2433,2435,2440,2441,2443,2445,2447,2448,2451,2452,2454,2457,2459,2462,2464,2466,2468,2469,2471,2472,2474,2476,2477,2478,2480,2481,2483,2485,2486,2488,2491,2492,2494,2496,2500,2501,2508,2510,2511,2513,2514,2519,2520,2528,2530,2533,2538,2543,2630,2634,2635,2636,2637,2639,2640,2643,2652,2654,2772,2793,2794,2797,2799,2801,2802,2803,2806,2809,2821,2827,2829,2831,2835,2845,2849,2852,2854,2857,2859,2863,2989,2991,3001,3007,3016,3017,3020,3022,3026,3028,3031,3036,3039,3041,3042,3045,3054,3057,3058,3066,3069,3083,3203,3206,3215,3217,3223,3227,3229,3232,3236,3241,3245,3250,3253,3258,3383,3386,3387,3389,3399,3400,3410,3412,3414,3417,3418,3424,3427,3429,3432,3435,3507,3533,3548,3563,3570,3581,3582,3585,3586,3591,3594,3596,3600,3602,3603,3606,3607,3609,3615,3616,3618,3621,3622,3624,3627,3630,3632,3633,3634,3640,3641,3644,3645,3646,3648,3652,3666,3750,3751,3760,3761,3766,3774,3776,3778,3780,3781,3783,3785,3787,3788,3789,3792,3794,3796,3799,3800,3803,3805,3809,3815,3817,3822,3823,3826,3827,3829,3831,3838,3840,3843,3849,3854,3983,3986,3988,3989,3991,3992,3993,3998,4000,4001,4003,4005,4008,4011,4014,4017,4018,4021,4026,4030,4038,4040,4042,4045,4052,4054,4058,4060,4061,4063,4065,4067,4069,4070,4072,4074,4078,4082,4083,4086,4088,4094,4096,4097,4101,4104,4112,4114,4158,4200,4202,4204,4205,4207,4209,4212,4214,4218,4220,4222,4229,4231,4235,4237,4239,4240,4248,4251,4253,4254,4260,4263,4267,4270,4283,4406,4412,4415,4423,4426,4428,4430,4441,4447,4449,4451,4453,4456,4459,4460,4462,4464,4465,4467,4474,4476,4482,4485,4488,4492,4496,4497,4502,4520,4638,4639,4640,4641,4645,4647,4650,4653,4660,4669,4671,4674,4676,4677,4679,4683,4684,4690,4691,4693,4696,4805,4820,4823,4828,4836,4839,4864,4870,4871,4873,4878,4879,5009,5013,5026,5029,5031,5032,5034,5039,5042,5049,5050,5051,5054,5057,5065,5069,5072,5208,5210,5216,5221,5225,5227,5236,5240,5242,5250,5252,5255,5260,5264,5266,5300,5413,5431,5433,5436,5438,5445,5451,5456,5464,5594,5612,5651,5803,5807,5809,5820,5823,5826,5828,5829,5831,5832,5834,5835,5843,5847,5851,5853,5865,5867,5869,5872,5874,5875,5877,5882,5889,6008,6014,6018,6022,6023,6025,6026,6028,6031,6033,6036,6040,6041,6044,6047,6048,6052,6055,6057,6061,6062,6069,6073,6077,6079,6080,6081,6084,6087,6090,6091,6094,6097,6100,6103,6107,6115,6216,6223,6226,6227,6229,6231,6242,6245,6247,6248,6249,6251,6253,6255,6259,6260,6264,6266,6276,6277,6279,6282,6284,6287,6288,6290,6421,6442,6447,6451,6459,6461,6464,6469,6470,6473,6481,6482,6484,6487,6627,6639,6641,6646,6648,6653,6656,6658,6660,6663,6664,6665,6667,6668,6671,6672,6673,6675,6679,6680,6682,6687,6691,6693,6696,6698,6713,6750,6836,6838,6842,6844,6846,6847,6850,6852,6854,6860,6864,6867,6872,6874,6878,6879,6881,6882,6884,6886,6889,6891,6893,6896,6899,6900,6902,6906,6913,6914,6921,6922,6925,6928,6932,7036,7039,7040,7042,7047,7055,7056,7059,7062,7066,7067,7072,7073,7080,7083,7096,7099,7103,7107,7199,7202,7221,7229,7232,7235,7239,7241,7244,7245,7247,7252,7259,7262,7268,7269,7271,7272,7274,7276,7278,7315,7318,7394,7406,7420,7423,7425,7436,7437,7439,7442,7444,7446,7448,7450,7452,7455,7457,7463,7472,7474,7478,7513,7614,7616,7618,7622,7625,7629,7630,7632,7634,7635,7652,7666,7792,7796,7800,7803,7805,7808,7839,7840,7844,7915,7987,8018,8021,8023,8025,8026,8028,8029,8030,8033,8035,8037,8048,8203,8206,8207,8213,8220,8225,8227,8230,8234,8240,8245,8247,8250,8252,8256,8257,8269,8386,8393,8395,8397,8398,8401,8403,8405,8407,8408,8411,8413,8415,8418,8419,8421,8423,8430,8431,8434,8435,8438,8564,8582,8594,8597,8599,8601,8603,8607,8608,8615,8622,8632,8639,8645,8656,8756,8775,8777,8938,8940,8944,8947,8955,8967,8970,8973,8979,8981,8984,8986,9266,9278,9452,9453,9455,9457,9460,9461,9466,9471,9472,9475,9479,9491,9493,9495,9604,9624,9630,9633,9634,9636,9641,9647,9651,9654,9656,9657,9658,9662,9665,9669,9673,9677,9679,9682,9690,9771,9830,9832,9834,9835,9837,9841,9844,9849,9853,9991,10006,10012,10014,10018,10020,10155,10159,10171,10176,10178,10180,10182,10183,10184,10185,10189,10190,10196,10199,10201,10202,10205,10208,10209,10211,10213,10214,10215,10216,10218,10220,10223,10226,10227,10228,10232,10234,10235,10238,10242,10245,10248,10249,10252,10253,10255,10257,10258,10260,10261,10265,10266,10268,10269,10271,10274,10275,10276,10284,10286,10289,10299,10547,10550,10552,10556,10561,10564,10566,10567,10584,10593,10611,10722,10728,10731,10744,10750,10764,10767,10768,10771,10772,10774,10775,10778,10779,10781,10795,10932,10933,10936,10937,10942,10943,10945,10946,10948,10951,10953,10956,10962,10963,10965,10979,10981,10984,11112,11126,11129,11131,11132,11136,11140,11145,11147,11149,11150,11300,11301,11303,11306,11308,11311,11312,11314,11317,11327,11329,11332,11343,11491,11494,11496,11498,11499,11502,11503,11505,11507,11508,11510,11514,11517,11520,11529,11531,11534,11538,11541,11542,11546,11547,11553,11670,11675,11677,11679,11681,11683,11686,11687,11690,11697,11699,11701,11705,11709,11716,11718,11721,11725,11728,11730,11732,11736,11861,11867,11872,11878,11882,11885,11887,11888,11889,11893,11894,11898,11910,11914,11926,11928,11931,12046,12072,12084,12087,12090,12105,12109,12248,12261,12263,12265,12271,12279,12450,12451,12454,12458,12460,12464,12469,12478,12480,12486,12513,12632,12639,12641,12645,12649,12653,12654,12656,12657,12661,12663,12668,12669,12677,12680,12684,12685,12687,12690,12693,12696,12699,12703,12827,12838,12850,12852,12855,12868,12869,12871,12875,12879,12884,12889,13002,13037,13045,13046,13048,13051,13052,13065,13072,13073,13075,13076,13078,13080,13082,13086,13087,13091,13095,13097,13099,13100,13102,13113,13118,13124,13131,13239,13242,13244,13246,13247,13248,13254,13257,13259,13260,13261,13264,13265,13267,13270,13272,13276,13278,13283,13285,13292,13403,13417,13421,13424,13425,13427,13432,13434,13436,13440,13443,13446,13449,13453,13459,13460,13464,13465,13466,13468,13469,13471,13475,13481,13488,13489,13492,13496,13498,13499,13501,13505,13508,13626,13630,13646,13649,13650,13659,13662,13781,13809,13813,13817,13818,13820,13823,13824,13827,13828,13832,13833,13836,13838,13841,13849,13851,13854,13855,13873,13876,13877,13879,13882,13886,13887,13892,13903,13907,13909,13910,13912,13914,13917,13928,13996,14013,14015,14017,14023,14026,14027,14029,14035,14039,14041,14043,14047,14050,14052,14054,14056,14057,14061,14065,14069,14071,14074,14076,14078,14079,14084,14085,14086,14089,14091,14095,14099,14101,14113,14115,14118,14123,14211,14217,14253,14255,14256,14259,14261,14262,14265,14267,14268,14271,14272,14273,14276,14278,14282,14283,14285,14312,14459,14461,14462,14465,14470,14472,14485,14487,14492,14495,14636,14641,14644,14647,14648,14650,14651,14653,14654,14660,14661,14666,14667,14673,14676,14679,14680,14717,14827,14831,14835,14838,14841,14846,14849,14862,14865,14869,14875,14877,14878,14881,14892,14894,14896,15038,15046,15048,15052,15054,15056,15058,15059,15061,15063,15233,15388,15395,15396,15399,15401,15402,15403,15407,15411,15425,15432,15433,15568,15572,15581,15583,15585,15586,15588,15595,15598,15600,15605,15610,15618,15621,15623,15752,15755,15758,15760,15762,15766,15768,15770,15772,15773,15775,15779,15782,15787,15790,15794,15800,15801,15804,15806,15810,15812,15818,15821,15823,15825,15828,15830,15838,15841,15846,15848,15984,15990,16000,16004,16006,16007,16012,16013,16015,16018,16019,16021,16022,16025,16027,16028,16032,16045,16170,16173,16177,16188,16190,16194,16200,16202,16209,16211,16214,16224,16226,16233,16235,16238,16243,16255,16260,16382,16385,16389,16392,16395,16398,16402,16416,16418,16422,16586,16589,16597,16599,16605,16607,16706,16709,16746,16748,16754,16755,16760,16764,16767,16769,16777,16789,16800,16807,16937,16949,16951,16953,16955,16957,16958,16961,16967,16969,16973,16976,16986,16989,16999,17004,17010,17016,17021,17129,17149,17150,17157,17159,17160,17167,17169,17173,17176,17177,17182,17185,17186,17188,17189,17191,17194,17455,17457,17460,17463,17466,17479,17481,17483,17484,17485,17486,17487,17489,17493,17494,17497,17498,17503,17504,17520,17521,17522,17524,17526,17528,17540,17547,17549,17550,17555,17556,17561,17563,17566,17568,17570,17572,17574,17576,17578,17579,17581,17587,17590,17599,17694,17698,17700,17704,17708,17713,17716,17718,17722,17725,17726,17728,17729,17730,17733,17737,17747,17752,17758,17762,17763,17767,17768,17769,17771,17774,17778,17789,17892,17895,17900,17902,17903,17915,17923,17927,17929,17944,17946,18089,18091,18094,18095,18098,18100,18106,18113,18119,18126,18128,18130,18276,18278,18281,18282,18286,18294,18306,18311,18314,18459,18464,18472,18498,18648,18655,18658,18660,18662,18663,18665,18666,18667,18669,18671,18673,18675,18677,18681,18683,18686,18688,18693,18694,18697,18700,18702,18716,18720,18723,18834,18837,18856,18860,18862,18865,18869,18872,18873,18875,18876,18880,18884,18886,18890,18893,18895,18896,18897,18902,18915,18918,18921,18925,18927,18929,18934,19048,19060,19082,19083,19086,19089,19095,19105,19110,19112,19251,19254,19260,19261,19263,19267,19298,19301,19304,19318,19320,19441,19443,19452,19455,19461,19464,19465,19467,19469,19472,19475,19486,19489,19491,19492,19495,19497,19498,19500,19503,19504,19516,19652,19654,19658,19661,19663,19665,19682,19696,19697,19702,19703,19705,19846,19865,19866,19868,19870,19871,19876,19891,19892,19893,19894,19895,19896,19900,19904,19909,19912,19914,20068,20070,20072,20074,20078,20081,20084,20085,20086,20091,20093,20094,20097,20100,20103,20107,20109,20111,20121,20123,20126,20127,20130,20257,20258,20262,20270,20273,20277,20280,20282,20289,20292,20298,20305,20312,20322,20471,20476,20479,20480,20482,20483,20485,20487,20489,20490,20497,20634,20639,20644,20654,20656,20659,20660,20662,20664,20668,20670,20672,20674,20675,20678,20681,20686,20688,20691,20693,20694,20695,20696,20716,20728,20836,20843,20854,20855,20858,20860,20862,20865,20867,20872,20874,20876,20877,20880,20881,20885,20886,20888,20893,20899,20904,20905,20906,20907,20910,20911,20912,20914,20915,20916,20918,20919,20921,20922,20923,20926,20927,20928,20930,20933,20935,20941,20942,20944,20949,20951,20952,20953,20954,20955,20958, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 128,0,128,0 Ignored m6A +ptg000001l 23717 42702 5mC 0 + 23717 42702 139,69,19,1 145 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,50,98,136,138,168,176,729,732,735,744,772,781,802,874,878,898,922,937,2044,2437,2454,2651,2868,2876,2931,2970,2990,2994,3036,3047,3067,3125,3648,4152,4190,4463,5330,5498,5505,6728,6850,6961,7070,7081,7159,7511,8223,8402,8957,9474,9659,9661,10161,10448,10770,11587,11746,11951,12370,12859,13506,13520,13529,13682,14011,14192,15064,15410,15590,15858,15987,15995,16186,16191,16288,16290,16377,16419,16422,16443,16452,16455,16457,16468,16490,16492,16496,16509,16511,16515,16520,16524,16526,16537,16556,16561,16566,16573,16585,16590,16595,16598,16600,16663,16665,16672,16679,16686,16794,16806,16816,16823,16825,16901,16973,17022,17070,17268,17646,17767,17799,17809,17851,17858,17867,17895,17906,17996,17998,18002,18050,18613,18621,18682,18751,18757,18787,18795,18800,18804,18811,18855,18952,18984, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 23773 44597 NUC 0 + 23773 44597 169,169,169,1 102 148,132,120,126,136,153,111,134,109,136,175,104,86,117,125,119,124,100,83,128,122,117,108,129,135,146,129,151,118,100,130,139,122,103,91,75,135,125,142,154,116,125,99,160,279,173,108,139,137,134,247,110,136,127,149,147,116,124,140,138,170,145,123,146,107,110,117,146,87,146,140,146,141,169,154,134,128,135,124,121,163,98,129,107,260,94,102,142,145,144,149,110,113,138,120,135,140,153,126,148,136,107, 0,208,413,592,755,997,1195,1428,1656,1851,2012,2211,2532,2643,2852,3072,3247,3421,3655,3843,4272,4509,4685,4868,5061,5255,5453,5640,5878,6104,6279,6476,6702,6921,7096,7307,7467,7655,7833,8037,8258,8427,8645,8766,8975,9267,9484,9679,9842,10009,10288,10600,10784,10973,11139,11332,11542,11725,11920,12098,12268,12475,12692,12879,13120,13281,13497,13651,14112,14301,14484,14669,14885,15052,15222,15422,15612,15837,16034,16249,16411,16596,16796,17010,17183,17588,17778,17935,18119,18303,18487,18712,18923,19101,19309,19505,19694,19903,20119,20311,20486,20717, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 169,169,169,0 Ignored NUC +ptg000001l 26088 26305 FIRE 0 + 26088 26305 175,0,0,1 1 217, 0, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 175,0,0,0 Ignored FIRE +ptg000001l 27744 28045 FIRE 0 + 27744 28045 255,0,0,1 1 301, 0, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 33916 34061 FIRE 0 + 33916 34061 255,140,0,1 1 145, 0, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 255,140,0,0 Ignored FIRE +ptg000001l 23761 44490 LINKER 0 + 23761 44490 147,112,219,1 99 12,60,73,59,37,106,45,122,94,86,25,24,25,92,95,56,50,134,105,115,59,75,64,59,52,58,87,108,75,67,87,97,72,120,85,53,53,62,67,53,93,22,49,13,44,87,24,30,65,74,53,39,44,63,67,71,38,32,37,72,63,95,54,106,37,315,102,37,45,70,26,1,46,56,97,62,91,41,22,102,85,66,145,96,55,42,39,40,76,101,65,70,76,54,69,63,66,27,95, 0,160,352,545,730,903,1162,1318,1574,1777,1999,2199,2630,2772,2989,3203,3383,3533,3750,4406,4638,4805,5009,5208,5413,5594,5803,6008,6216,6421,6627,6836,7036,7199,7394,7614,7792,7987,8203,8386,8564,8756,8938,9266,9452,9604,9830,9991,10547,10722,10932,11112,11300,11491,11670,11861,12072,12248,12450,12632,12827,13037,13239,13403,13626,13809,14211,14459,14636,14827,15038,15233,15388,15568,15752,15984,16170,16382,16586,16706,16937,17129,17455,17694,17892,18089,18276,18459,18648,18834,19048,19251,19441,19652,19846,20068,20257,20471,20634, ptg000001l:23691-44729:m54329U_210323_190418/85459034/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 25192 34140 m6A 0 + 25192 34140 128,0,128,1 327 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,2,3,6,8,15,20,24,27,29,32,34,36,40,55,60,181,196,198,208,210,214,218,229,232,233,236,239,244,254,261,410,411,414,416,417,424,425,427,429,431,435,575,583,604,640,813,959,985,991,993,1002,1009,1021,1180,1334,1341,1342,1344,1531,1747,1908,1909,2068,2069,2071,2074,2076,2079,2217,2221,2230,2235,2239,2240,2243,2244,2248,2250,2254,2258,2260,2266,2273,2276,2278,2281,2283,2289,2409,2421,2429,2431,2439,2447,2454,2455,2460,2464,2471,2590,2593,2595,2599,2602,2607,2609,2611,2613,2614,2617,2621,2623,2627,2630,2638,2639,2641,2643,2647,2651,2652,2657,2800,2806,2808,2809,2812,2813,2820,2832,2992,3010,3020,3022,3031,3185,3187,3197,3216,3222,3229,3392,3393,3397,3416,3582,3584,3595,3598,3600,3601,3603,3608,3620,3626,3759,3768,3777,3779,3782,3783,3785,3788,3790,3792,3955,3960,4159,4163,4167,4190,4319,4324,4329,4332,4337,4507,4531,4534,4540,4542,4545,4548,4706,4710,4723,4761,4920,4959,5107,5110,5112,5120,5291,5292,5294,5297,5299,5301,5303,5306,5309,5313,5315,5317,5323,5332,5465,5485,5490,5491,5492,5494,5497,5501,5503,5507,5512,5518,5524,5665,5672,5675,5676,5681,5687,5699,5701,5705,5707,5708,5710,5712,5717,5723,5726,5876,5880,5883,5884,5886,5887,5889,5891,5892,5893,5896,5904,5917,5920,5934,6233,6235,6237,6238,6240,6409,6552,6590,6751,6752,6753,6758,6759,6761,6762,6772,6779,6943,6964,6967,7102,7328,7597,7598,7601,7604,7766,7770,7773,7931,7949,7956,8117,8120,8129,8259,8267,8281,8288,8293,8295,8297,8300,8301,8303,8305,8311,8467,8475,8480,8488,8651,8654,8663,8683,8688,8689,8691,8695,8697,8698,8700,8712,8719,8724,8726,8728,8732,8910,8912,8921,8924,8933,8939,8947, ptg000001l:25050-34283:m54329U_210323_190418/43059336/ccs block 128,0,128,0 Ignored m6A +ptg000001l 26171 34166 5mC 0 + 26171 34166 139,69,19,1 40 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,197,414,422,430,454,460,477,536,540,593,627,638,640,671,1194,1201,1272,1707,2407,2834,2838,2865,2876,3763,4057,4184,4274,4445,4499,4616,4705,5769,5948,6112,6591,7205,7207,7707,7994, ptg000001l:25050-34283:m54329U_210323_190418/43059336/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 25253 34102 NUC 0 + 25253 34102 169,169,169,1 46 120,148,139,172,145,158,153,186,215,160,158,137,119,118,142,159,153,162,165,132,162,198,128,169,157,158,147,170,132,140,149,298,168,142,160,163,134,225,268,161,157,160,129,155,162,177, 0,201,375,580,753,961,1120,1284,1471,1687,1849,2019,2229,2411,2597,2772,2971,3169,3356,3566,3732,3900,4130,4277,4488,4701,4899,5060,5272,5464,5666,5874,6180,6349,6530,6719,6907,7042,7268,7544,7713,7896,8069,8251,8428,8672, ptg000001l:25050-34283:m54329U_210323_190418/43059336/ccs block 169,169,169,0 Ignored NUC +ptg000001l 25192 34140 LINKER 0 + 25192 34140 147,112,219,1 47 61,81,26,66,1,63,1,11,1,1,2,12,73,63,68,33,40,45,25,45,34,6,32,19,42,56,40,14,42,60,62,59,8,1,39,29,25,1,1,8,8,26,13,53,22,82,38, 0,181,410,575,813,959,1180,1334,1531,1747,1908,2068,2217,2409,2590,2800,2992,3185,3392,3582,3759,3955,4159,4319,4507,4706,4920,5107,5291,5465,5665,5876,6233,6409,6552,6751,6943,7102,7328,7597,7766,7931,8117,8259,8467,8651,8910, ptg000001l:25050-34283:m54329U_210323_190418/43059336/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 25965 39680 m6A 0 - 25965 39680 128,0,128,1 755 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,186,205,209,210,212,215,218,220,227,229,374,539,546,569,571,734,737,739,750,753,755,758,934,936,938,943,944,946,948,963,974,1103,1115,1119,1121,1122,1124,1127,1128,1131,1132,1135,1136,1138,1142,1153,1156,1180,1182,1183,1187,1344,1348,1351,1356,1357,1359,1363,1366,1387,1556,1562,1563,1570,1574,1701,1721,1723,1726,1734,1736,1739,1740,1742,1744,1749,1767,1916,1919,1922,1926,1929,1932,1933,1935,1939,1941,1947,1950,1951,1954,1956,1958,1966,1988,1991,1996,2097,2103,2105,2106,2109,2111,2113,2115,2132,2138,2142,2145,2147,2152,2155,2173,2176,2178,2180,2316,2320,2327,2341,2345,2346,2347,2351,2515,2518,2523,2525,2531,2533,2534,2537,2545,2550,2555,2725,2727,2729,2731,2744,2752,2753,2896,2898,2913,2926,2927,2929,2932,2934,2938,2939,2958,2959,2964,2967,2970,2972,2974,2977,2978,2986,2997,3000,3135,3137,3150,3290,3309,3311,3312,3315,3333,3335,3357,3360,3505,3508,3513,3516,3653,3676,3678,3685,3837,3840,3843,3844,3848,3851,3853,3857,3858,3862,3865,3868,3873,3875,3876,3880,3883,3972,3978,3988,4006,4007,4010,4012,4013,4019,4023,4025,4027,4028,4034,4037,4038,4041,4045,4047,4049,4056,4063,4080,4083,4086,4236,4250,4252,4253,4255,4258,4260,4265,4269,4271,4278,4280,4283,4418,4420,4423,4435,4437,4440,4442,4446,4447,4449,4452,4454,4456,4460,4461,4463,4464,4467,4469,4476,4478,4483,4489,4492,4632,4634,4638,4640,4646,4799,4801,4814,4826,4827,4830,4832,4836,4838,4843,4846,4849,4984,4991,4995,4998,5009,5014,5017,5020,5022,5025,5028,5031,5035,5041,5043,5048,5204,5210,5211,5214,5216,5219,5221,5418,5446,5599,5601,5603,5606,5607,5779,5782,5783,5785,5792,5793,5796,5798,5817,5994,5999,6002,6170,6182,6186,6187,6189,6191,6193,6194,6207,6211,6214,6215,6360,6363,6390,6395,6397,6399,6404,6550,6553,6555,6556,6560,6568,6571,6573,6576,6578,6734,6736,6737,6741,6743,6751,6755,6758,6759,6763,6766,6769,6771,6774,6775,6777,6778,6780,6782,6785,6787,6789,6792,6796,6802,6803,6804,6808,6812,6818,6825,6951,6957,6961,6970,6973,6974,6975,6977,6979,6983,6986,6988,6992,6998,7000,7002,7012,7152,7158,7159,7182,7183,7187,7189,7208,7221,7223,7225,7228,7344,7347,7371,7383,7395,7400,7401,7404,7417,7422,7426,7437,7569,7570,7572,7574,7585,7587,7588,7590,7591,7595,7597,7598,7602,7616,7638,7640,7642,7645,7649,7796,7802,7804,7808,7810,7814,7838,7844,8028,8179,8180,8184,8196,8205,8210,8220,8222,8224,8227,8410,8413,8415,8417,8420,8425,8436,8439,8457,8459,8461,8463,8627,8632,8634,8636,8639,8641,8645,8655,8658,8670,8676,8680,8682,8873,8964,9033,9035,9037,9050,9061,9066,9068,9071,9083,9090,9097,9102,9104,9107,9108,9271,9287,9290,9310,9314,9330,9466,9473,9475,9479,9501,9633,9644,9650,9653,9823,9825,9831,9834,9835,9837,9839,9842,9845,9847,9852,9855,9858,9861,9868,9880,9883,9948,9987,9989,9993,10048,10055,10057,10059,10061,10099,10110,10138,10140,10141,10228,10231,10234,10235,10403,10405,10409,10415,10417,10419,10421,10422,10423,10428,10432,10435,10437,10442,10445,10449,10450,10452,10453,10457,10459,10462,10464,10465,10467,10473,10476,10480,10492,10621,10624,10634,10640,10648,10651,10655,10664,10667,10671,10988,10990,10993,10995,10996,10999,11004,11009,11011,11014,11016,11022,11179,11181,11185,11186,11189,11191,11193,11195,11199,11203,11206,11208,11211,11213,11217,11220,11221,11223,11228,11381,11395,11401,11411,11422,11436,11553,11566,11571,11577,11584,11589,11591,11594,11596,11603,11609,11613,11614,11619,11624,11632,11777,11780,11781,11783,11786,11788,11792,11798,11799,11805,11946,11953,11972,11979,11986,12126,12129,12130,12136,12153,12156,12159,12162,12165,12172,12175,12177,12178,12180,12182,12184,12189,12328,12329,12335,12342,12345,12347,12349,12350,12353,12366,12367,12369,12371,12374,12377,12379,12382,12385,12386,12404,12407,12547,12549,12552,12555,12556,12558,12574,12579,12584,12587,12591,12593,12607,12623,12624,12627,12631,12635,12745,12748,12756,12762,12764,12769,12772,12774,12777,12928,12944,12945,12946,12950,12952,12958,12962,12965,12979,12983,13116,13143,13147,13150,13152,13157,13170,13319,13320,13341,13490,13664,13668,13674,13675,13680,13711,13714, ptg000001l:25868-39722:m54329U_210323_190418/10290844/ccs block 128,0,128,0 Ignored m6A +ptg000001l 26155 39309 5mC 0 - 26155 39309 139,69,19,1 58 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,17,214,431,439,494,533,553,557,610,644,688,1514,1715,2026,2424,2882,2893,3068,3797,4291,4413,4446,4462,4613,4722,5074,5786,5965,6520,6831,7133,7165,7222,7224,7308,7329,7666,8011,8383,9150,9309,9319,9451,9507,9514,9933,9998,10484,10888,11069,11113,11123,11127,11574,11665,12171,13153, ptg000001l:25868-39722:m54329U_210323_190418/10290844/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 25966 39629 NUC 0 - 25966 39629 169,169,169,1 69 185,144,164,162,175,128,156,168,126,148,100,135,163,169,142,134,139,144,136,151,88,149,134,139,152,134,155,196,152,171,176,167,144,145,155,125,139,115,131,146,183,150,182,163,190,159,162,135,131,169,103,86,167,128,316,156,152,116,144,140,139,138,139,109,150,132,148,148,173, 0,229,374,571,758,974,1187,1387,1574,1767,1996,2180,2351,2555,2753,3000,3150,3360,3516,3685,3883,4086,4283,4492,4646,4849,5048,5221,5446,5607,5817,6002,6215,6404,6578,6825,7012,7228,7437,7649,7844,8028,8227,8463,8682,8873,9108,9330,9501,9653,9883,10141,10235,10492,10671,11022,11228,11436,11632,11805,11986,12189,12407,12635,12777,12983,13170,13341,13490, ptg000001l:25868-39722:m54329U_210323_190418/10290844/ccs block 169,169,169,0 Ignored NUC +ptg000001l 36368 36458 FIRE 0 - 36368 36458 255,140,0,1 1 90, 0, ptg000001l:25868-39722:m54329U_210323_190418/10290844/ccs block 255,140,0,0 Ignored FIRE +ptg000001l 25965 39456 LINKER 0 - 25965 39456 147,112,219,1 68 1,44,1,33,25,41,85,44,19,67,81,84,36,41,29,105,16,71,12,33,47,115,48,75,15,51,65,18,29,9,39,9,46,45,29,92,62,77,94,81,49,1,49,54,56,1,76,60,36,21,61,155,8,51,35,50,56,80,29,41,64,80,89,33,56,55,23,1, 0,186,374,539,734,934,1103,1344,1556,1701,1916,2097,2316,2515,2725,2896,3135,3290,3505,3653,3837,3972,4236,4418,4632,4799,4984,5204,5418,5599,5779,5994,6170,6360,6550,6734,6951,7152,7344,7569,7796,8028,8179,8410,8627,8873,9033,9271,9466,9633,9823,9987,10228,10621,10988,11179,11381,11553,11777,11946,12126,12328,12547,12745,12928,13116,13319,13490, ptg000001l:25868-39722:m54329U_210323_190418/10290844/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 28310 44103 m6A 0 - 28310 44103 128,0,128,1 2092 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,1,2,6,122,130,136,142,144,147,160,161,168,170,173,174,176,178,180,186,188,319,321,324,327,329,330,332,335,337,339,346,363,365,367,368,373,377,380,382,384,386,388,390,399,404,408,411,414,544,548,549,551,553,565,566,568,569,580,581,582,584,587,594,701,703,710,723,725,730,734,736,739,740,741,743,745,747,750,751,753,755,756,759,761,766,772,773,777,790,792,915,936,940,941,945,946,958,960,968,1061,1087,1094,1102,1107,1116,1117,1124,1131,1156,1229,1232,1235,1242,1244,1246,1248,1251,1252,1254,1256,1258,1260,1261,1264,1266,1267,1268,1271,1274,1277,1279,1280,1282,1283,1285,1286,1287,1289,1290,1292,1294,1298,1302,1304,1308,1310,1311,1316,1318,1321,1325,1328,1333,1340,1342,1448,1450,1459,1463,1465,1466,1467,1469,1473,1474,1476,1477,1479,1482,1484,1487,1490,1491,1492,1495,1498,1499,1503,1506,1508,1512,1513,1517,1520,1523,1524,1528,1530,1531,1532,1535,1538,1541,1542,1545,1548,1551,1554,1558,1560,1566,1570,1574,1576,1581,1588,1619,1627,1643,1667,1682,1687,1692,1693,1695,1696,1700,1702,1704,1706,1710,1711,1716,1718,1723,1725,1727,1730,1733,1735,1738,1739,1741,1746,1748,1755,1757,1758,1760,1762,1764,1891,1898,1902,1905,1907,1908,1910,1912,1913,1915,1917,1920,1922,1924,1926,1930,1932,1935,1938,1940,1944,1947,1955,1964,2064,2073,2075,2078,2080,2090,2092,2097,2099,2104,2107,2109,2111,2115,2116,2118,2119,2123,2124,2133,2138,2144,2166,2261,2265,2287,2289,2295,2297,2298,2301,2303,2305,2311,2315,2318,2454,2456,2474,2481,2482,2485,2487,2490,2498,2633,2634,2635,2639,2643,2650,2652,2653,2656,2658,2661,2664,2667,2669,2671,2673,2675,2676,2677,2680,2683,2686,2692,2828,2839,2845,2857,2863,2871,2874,2876,3068,3071,3084,3088,3089,3093,3095,3243,3251,3254,3256,3262,3271,3273,3275,3277,3284,3287,3288,3290,3291,3295,3318,3447,3469,3472,3474,3611,3612,3616,3623,3625,3627,3634,3649,3654,3658,3661,3662,3664,3770,3777,3780,3796,3798,3800,3802,3803,3805,3808,3812,3814,3815,3819,3821,3825,3827,3833,3834,3837,3839,3842,3844,3846,3848,3849,3856,3976,3982,3984,3986,3987,3988,3991,3993,4000,4002,4006,4007,4009,4012,4013,4015,4017,4018,4019,4021,4022,4023,4027,4029,4030,4033,4052,4142,4156,4167,4168,4171,4172,4174,4176,4177,4179,4180,4181,4183,4184,4185,4187,4189,4191,4192,4193,4195,4197,4198,4199,4201,4204,4205,4207,4208,4210,4211,4215,4217,4218,4219,4220,4222,4223,4224,4226,4228,4229,4231,4233,4235,4238,4243,4245,4246,4249,4251,4254,4256,4263,4265,4267,4270,4273,4275,4278,4280,4288,4290,4406,4410,4413,4418,4421,4424,4426,4429,4430,4432,4433,4435,4437,4440,4444,4447,4448,4451,4457,4458,4459,4463,4465,4467,4577,4603,4606,4608,4612,4620,4623,4625,4628,4629,4630,4632,4633,4638,4641,4643,4648,4652,4653,4655,4774,4781,4786,4788,4789,4790,4792,4794,4795,4800,4801,4803,4804,4806,4807,4808,4810,4813,4814,4949,4971,4973,4979,4981,4985,4987,4989,4993,4995,4998,5002,5008,5011,5014,5023,5024,5026,5028,5031,5141,5161,5163,5166,5170,5175,5177,5179,5182,5183,5185,5187,5190,5191,5193,5344,5347,5349,5350,5357,5360,5362,5364,5365,5370,5372,5381,5384,5390,5393,5396,5398,5399,5402,5404,5405,5409,5411,5414,5415,5418,5419,5421,5422,5425,5426,5545,5548,5553,5557,5559,5560,5561,5563,5565,5570,5571,5573,5577,5579,5580,5582,5583,5584,5589,5594,5601,5606,5610,5696,5699,5700,5706,5708,5709,5711,5712,5725,5727,5730,5731,5735,5737,5740,5741,5744,5745,5748,5750,5754,5755,5759,5761,5762,5764,5766,5768,5769,5771,5772,5774,5775,5778,5780,5781,5783,5784,5785,5792,5801,5803,5806,5808,5810,5811,5821,5936,5940,5943,5945,5952,5953,5954,5957,5959,5960,5962,5965,5966,5972,5975,5977,5978,5981,5982,5984,5986,5989,5990,5993,5996,6003,6005,6070,6080,6096,6112,6114,6116,6118,6122,6130,6134,6136,6137,6140,6143,6149,6150,6153,6156,6159,6163,6166,6168,6179,6259,6262,6263,6265,6269,6271,6275,6278,6282,6287,6289,6291,6292,6294,6296,6298,6300,6304,6306,6309,6310,6313,6316,6319,6322,6325,6327,6329,6331,6334,6335,6337,6338,6341,6347,6348,6350,6353,6355,6356,6359,6360,6375,6388,6390,6391,6407,6409,6411,6413,6414,6416,6417,6419,6428,6430,6431,6432,6435,6460,6577,6580,6583,6604,6606,6607,6609,6615,6617,6619,6624,6626,6630,6631,6632,6636,6640,6641,6642,6643,6646,6650,6652,6656,6657,6658,6660,6662,6668,6669,6670,6673,6676,6677,6678,6683,6684,6688,6690,6692,6693,6697,6809,6811,6812,6815,6817,6820,6824,6825,6827,6828,6829,6833,6835,6837,6840,6847,6852,6854,6856,6862,6864,6866,6868,6870,6872,6965,6989,6992,6993,6997,6998,7000,7004,7008,7010,7011,7014,7016,7018,7021,7023,7025,7029,7030,7033,7035,7037,7040,7042,7045,7047,7049,7050,7054,7059,7060,7062,7064,7066,7067,7068,7070,7073,7074,7076,7078,7187,7193,7197,7200,7203,7204,7206,7209,7212,7214,7217,7219,7220,7223,7225,7228,7229,7232,7236,7238,7240,7244,7246,7247,7248,7254,7355,7361,7364,7368,7372,7375,7377,7379,7380,7382,7383,7387,7389,7391,7392,7394,7397,7401,7405,7407,7409,7411,7414,7415,7417,7419,7423,7425,7441,7450,7453,7455,7456,7459,7461,7465,7467,7469,7474,7480,7607,7608,7610,7613,7615,7618,7619,7621,7623,7624,7626,7629,7630,7632,7635,7637,7638,7640,7644,7650,7771,7789,7793,7795,7796,7798,7801,7803,7808,7811,7813,7819,7821,7822,7825,7827,7828,7830,7836,7837,7839,7841,7847,7848,7849,7851,7859,7862,7864,7865,7980,7985,7992,7995,8000,8003,8010,8011,8012,8015,8019,8035,8042,8045,8047,8175,8179,8184,8185,8189,8191,8194,8195,8199,8320,8322,8326,8330,8335,8340,8353,8354,8356,8365,8368,8370,8373,8374,8376,8378,8381,8384,8385,8386,8389,8390,8392,8394,8397,8399,8401,8402,8405,8408,8410,8414,8416,8417,8419,8421,8422,8424,8426,8427,8428,8429,8431,8432,8436,8440,8442,8443,8444,8447,8449,8451,8453,8455,8457,8461,8463,8465,8471,8473,8587,8590,8592,8594,8595,8597,8599,8602,8603,8613,8614,8615,8617,8620,8621,8623,8627,8629,8630,8631,8632,8633,8636,8637,8638,8643,8645,8648,8650,8651,8654,8659,8664,8666,8669,8671,8672,8675,8677,8690,8693,8695,8699,8705,8828,8830,8832,8834,8836,8840,8841,8844,8846,8848,8850,8854,8858,8861,8863,8864,8866,8868,8872,8875,8876,8878,8883,8885,8887,8890,8891,8894,8897,8900,8904,8907,8910,8911,8915,8920,8922,8926,8932,8943,9012,9028,9030,9033,9036,9044,9048,9049,9050,9052,9056,9060,9070,9072,9074,9077,9081,9084,9091,9097,9101,9198,9199,9202,9206,9208,9209,9211,9214,9215,9220,9221,9223,9225,9226,9230,9232,9234,9235,9237,9239,9241,9242,9244,9246,9249,9251,9252,9255,9256,9258,9260,9264,9268,9271,9274,9275,9278,9279,9281,9283,9284,9287,9289,9292,9294,9295,9300,9302,9305,9306,9312,9324,9328,9330,9436,9441,9443,9446,9447,9449,9453,9454,9466,9468,9474,9477,9478,9480,9485,9488,9598,9601,9608,9611,9613,9617,9620,9623,9627,9629,9632,9634,9637,9638,9641,9646,9647,9649,9651,9655,9657,9662,9765,9776,9777,9778,9781,9783,9784,9785,9788,9790,9791,9792,9794,9797,9798,9800,9801,9802,9806,9808,9809,9811,9812,9814,9815,9817,9819,9820,9822,9823,9825,9827,9830,9832,9833,9835,9837,9839,9842,9844,9847,9849,9857,9859,9861,9863,9864,9867,9868,9874,9876,9877,9878,9880,9884,9892,9896,9899,9915,9967,9984,9990,9992,9997,9998,10000,10002,10005,10008,10021,10029,10030,10032,10034,10037,10040,10041,10046,10048,10050,10055,10166,10170,10172,10173,10176,10178,10180,10182,10183,10186,10188,10189,10191,10194,10196,10198,10200,10201,10202,10204,10207,10210,10213,10219,10222,10227,10229,10234,10239,10242,10246,10359,10372,10375,10377,10380,10383,10385,10387,10391,10393,10394,10396,10400,10403,10404,10406,10407,10411,10415,10417,10419,10421,10424,10426,10427,10429,10432,10440,10443,10458,10477,10480,10482,10489,10558,10560,10564,10570,10571,10574,10575,10577,10578,10582,10583,10586,10587,10588,10593,10594,10596,10599,10600,10601,10605,10607,10611,10613,10617,10620,10621,10622,10625,10627,10629,10630,10632,10634,10636,10638,10643,10661,10665,10749,10752,10754,10756,10758,10760,10765,10767,10768,10771,10772,10776,10779,10780,10781,10784,10791,10795,10798,10802,10803,10805,10932,10934,10936,10945,10950,10953,10956,10963,10964,10969,10970,10971,10974,10975,10976,10978,10979,10980,10982,10983,10985,10986,10988,10990,10993,10995,10996,11010,11016,11017,11019,11022,11023,11026,11037,11039,11040,11042,11049,11164,11167,11169,11187,11188,11193,11196,11197,11199,11201,11203,11205,11206,11209,11211,11213,11220,11223,11224,11231,11234,11348,11350,11360,11363,11366,11369,11370,11373,11374,11375,11377,11378,11382,11384,11386,11388,11390,11392,11396,11398,11401,11404,11523,11524,11546,11549,11566,11569,11580,11582,11713,11719,11737,11743,11749,11751,11754,11756,11759,11761,11774,11861,11869,11873,11880,11887,11894,11902,11907,11909,11911,11914,11915,11921,11925,11939,11942,11947,11954,11960,11966,11971,11975,11978,11984,11988,11990,11995,12001,12017,12021,12024,12027,12035,12037,12040,12043,12050,12146,12148,12151,12153,12155,12157,12160,12161,12163,12165,12166,12172,12174,12175,12178,12182,12187,12189,12192,12193,12315,12317,12319,12322,12324,12327,12328,12332,12334,12335,12340,12344,12348,12352,12355,12359,12362,12363,12365,12366,12368,12371,12373,12377,12378,12508,12511,12513,12514,12518,12526,12532,12534,12538,12542,12544,12546,12661,12668,12685,12688,12690,12695,12698,12701,12703,12705,12710,12713,12715,12719,12723,12724,12725,12729,12734,12738,12741,12743,12746,12850,12853,12857,12860,12862,12864,12867,12869,12871,12872,12873,12876,12879,12881,12885,12888,12891,12892,12897,12900,12902,12905,12906,12908,12911,12912,12914,12916,12924,12928,12929,12930,12932,12934,12935,12936,12938,12940,12944,12945,12948,12951,13090,13096,13105,13116,13118,13180,13184,13248,13269,13280,13287,13289,13296,13299,13301,13307,13309,13311,13315,13317,13318,13321,13428,13432,13436,13438,13440,13443,13444,13446,13449,13451,13456,13461,13462,13468,13472,13473,13477,13481,13483,13484,13488,13490,13492,13497,13501,13505,13509,13514,13517,13520,13522,13525,13628,13639,13641,13645,13651,13658,13660,13661,13663,13665,13666,13668,13669,13671,13673,13674,13679,13680,13681,13683,13684,13690,13691,13694,13696,13698,13711,13727,13729,13842,13847,13850,13852,13853,13855,13856,13857,13858,13863,13868,13873,13874,13889,13897,14007,14008,14010,14012,14014,14017,14024,14025,14030,14038,14039,14045,14050,14051,14056,14059,14061,14062,14064,14065,14070,14075,14082,14188,14193,14198,14204,14214,14215,14217,14220,14223,14225,14228,14229,14230,14233,14235,14238,14242,14247,14248,14250,14251,14253,14254,14256,14257,14258,14260,14264,14266,14268,14269,14380,14400,14402,14405,14409,14415,14417,14419,14422,14423,14425,14427,14431,14432,14435,14436,14438,14588,14590,14592,14594,14596,14599,14602,14604,14606,14607,14614,14741,14747,14748,14749,14752,14755,14759,14762,14763,14765,14766,14769,14771,14772,14774,14777,14782,14784,14786,14787,14789,14790,14792,14794,14796,14797,14800,14801,14802,14804,14806,14809,14810,14814,14821,14823,14825,14829,14830,14831,14834,14838,14840,14841,14843,14844,14845,14848,14851,14852,14855,14856,14857,14859,14863,14876,14992,14997,14999,15001,15002,15004,15006,15015,15018,15021,15044,15175,15181,15185,15187,15197,15199,15200,15203,15204,15206,15207,15208,15210,15211,15216,15218,15221,15224,15225,15226,15227,15229,15232,15234,15239,15246,15247,15251,15253,15255,15257,15258,15261,15262,15267,15271,15272,15275,15277,15279,15280,15284,15297,15433,15435,15440,15441,15443,15450,15456,15466,15467,15562,15628,15629,15631,15633,15636,15640,15642,15644,15678,15779,15781,15792, ptg000001l:28298-44111:m54329U_210323_190418/2491749/ccs block 128,0,128,0 Ignored m6A +ptg000001l 28789 43585 5mC 0 - 28789 43585 139,69,19,1 102 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,434,1146,1567,1657,1779,1812,1870,1890,1999,3331,3876,4197,4246,4588,4590,4674,4695,5090,5377,6516,6743,6817,6873,6880,7299,7624,7850,7934,8395,8401,8435,8449,8458,8489,8493,8583,8611,8980,9031,9537,10023,10377,10494,10916,10924,11115,11120,11217,11219,11253,11348,11411,11438,11440,11444,11490,11495,11502,11514,11527,11529,11532,11536,11553,11581,11587,11592,11594,11601,11615,11646,11691,11723,11745,11752,11754,12229,12463,12575,12608,12738,12760,12780,12787,12796,12824,12915,12925,12927,12931,13686,13724,13729,13733,13784,13873,13881,13905,13935,13951,14795, ptg000001l:28298-44111:m54329U_210323_190418/2491749/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 28499 43938 NUC 0 - 28499 43938 169,169,169,1 74 130,129,106,122,118,100,105,126,99,94,135,134,135,191,147,128,136,105,119,89,115,135,118,134,109,150,118,85,114,79,116,111,92,108,100,126,120,114,127,120,113,122,96,105,109,102,110,112,83,126,114,113,118,130,86,95,121,129,114,103,138,129,106,102,112,109,105,110,149,126,115,130,135,160, 0,226,406,604,780,943,1154,1576,1776,1978,2130,2310,2504,2688,2907,3130,3286,3476,3668,3864,4102,4279,4467,4626,4843,5005,5238,5422,5633,5991,6272,6509,6684,6890,7066,7292,7462,7677,7859,8011,8285,8517,8913,9142,9300,9474,9867,10058,10477,10617,10861,11046,11216,11394,11586,11862,12005,12190,12358,12558,12763,12930,13133,13337,13541,13709,13894,14081,14250,14426,14688,14856,15109,15279, ptg000001l:28298-44111:m54329U_210323_190418/2491749/ccs block 169,169,169,0 Ignored NUC +ptg000001l 40171 40361 FIRE 0 - 40171 40361 200,0,0,1 1 190, 0, ptg000001l:28298-44111:m54329U_210323_190418/2491749/ccs block 200,0,0,0 Ignored FIRE +ptg000001l 36630 36784 FIRE 0 - 36630 36784 255,0,0,1 1 154, 0, ptg000001l:28298-44111:m54329U_210323_190418/2491749/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 28432 43955 LINKER 0 - 28432 43955 147,112,219,1 73 67,96,51,92,54,45,111,317,74,103,58,45,60,49,28,76,28,54,87,77,149,62,53,41,83,53,83,66,126,244,202,121,64,114,68,126,44,95,68,25,119,274,133,53,65,291,81,307,57,118,71,57,60,62,48,64,39,86,102,29,74,98,102,56,76,82,59,27,136,53,123,35,17, 0,197,422,579,793,965,1110,1326,1769,1942,2139,2332,2511,2706,2946,3121,3325,3489,3648,3854,4020,4284,4481,4652,4827,5019,5222,5423,5574,5814,6137,6455,6687,6843,7065,7233,7485,7649,7858,8053,8465,8706,9076,9314,9476,9643,10044,10237,10627,10810,11042,11226,11401,11591,12024,12193,12386,12539,12728,12968,13126,13306,13506,13720,13885,14066,14258,14466,14619,14870,15053,15311,15506, ptg000001l:28298-44111:m54329U_210323_190418/2491749/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 31498 49084 m6A 0 - 31498 49084 128,0,128,1 742 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,2,18,20,22,187,190,191,356,359,361,362,364,532,539,542,726,728,730,744,746,748,768,866,885,894,895,898,899,902,1079,1085,1090,1094,1225,1236,1242,1244,1247,1249,1253,1254,1256,1576,1596,1718,1720,1723,1724,1729,1734,1735,1738,1742,1744,1754,1756,1830,1914,1917,1919,1920,1921,1925,1926,1942,2097,2099,2109,2116,2493,2498,2501,2504,2505,2508,2511,2512,2515,2516,2518,2521,2523,2531,2534,2547,2706,2709,2710,2714,2906,2908,2914,3071,3074,3075,3077,3081,3083,3085,3087,3089,3090,3094,3099,3101,3103,3104,3106,3108,3112,3116,3118,3121,3122,3125,3126,3134,3137,3147,3327,3332,3333,3343,3500,3502,3504,3511,3517,3520,3528,3680,3690,3882,3886,3888,3890,4095,4100,4101,4102,4104,4111,4117,4120,4130,4271,4273,4277,4279,4281,4286,4290,4292,4293,4297,4299,4302,4304,4306,4309,4312,4472,4488,4491,4495,4497,4515,4677,4695,4847,4852,4857,4859,4870,4872,4876,4882,4884,4886,4889,4895,4899,4902,4904,4909,4917,5061,5064,5067,5075,5081,5088,5091,5098,5118,5234,5236,5240,5243,5248,5251,5252,5254,5256,5259,5261,5263,5265,5267,5275,5277,5281,5283,5285,5288,5289,5295,5300,5304,5305,5308,5309,5311,5314,5315,5322,5455,5466,5476,5549,5576,5582,5589,5591,5593,5594,5597,5616,5619,5620,5622,5623,5625,5628,5631,5637,5640,5642,5644,5646,5648,5652,5653,5656,5658,5660,5670,5673,5675,5833,5836,5837,5840,5842,5845,5846,5848,5851,5853,5856,5859,5862,5864,5868,5872,6038,6593,6596,6597,6600,6604,6606,6631,6632,6642,6767,6781,6786,6792,6795,6796,6800,6802,6804,6812,6814,6817,6820,6852,6980,6982,6984,6985,6990,6992,6994,6995,7000,7001,7003,7006,7008,7010,7013,7014,7016,7019,7046,7197,7208,7212,7215,7216,7218,7223,7229,7231,7233,7236,7238,7239,7241,7244,7289,7395,7398,7399,7412,7413,7417,7419,7425,7429,7433,7583,7781,7783,7807,7970,7972,7979,8160,8173,8178,8181,8182,8194,8196,8202,8204,8208,8213,8216,8369,8378,8381,8382,8566,8583,8586,8591,8593,8601,8603,8606,8612,8769,8772,8778,8783,8787,8790,8796,8800,8802,8963,8975,8978,8984,8990,8994,8998,8999,9001,9005,9017,9018,9023,9032,9174,9177,9178,9180,9183,9190,9196,9197,9200,9207,9209,9379,9382,9384,9390,9558,9577,9582,9583,9744,9750,9752,9756,9757,9771,9773,9917,9928,9930,9933,9939,9941,9945,9949,9955,9957,9959,9961,9963,9967,9971,9976,9979,9981,9985,9989,9996,10004,10135,10155,10158,10163,10165,10166,10176,10178,10182,10186,10187,10361,10382,10389,10393,10480,10539,10544,10545,10549,10551,10557,10743,10747,10749,10752,10928,10930,10932,10944,11104,11116,11119,11125,11128,11278,11281,11284,11286,11287,11290,11304,11307,11464,11471,11474,11478,11479,11483,11487,11488,11491,11493,11495,11498,11500,11518,11526,11692,11693,11704,11706,11872,11873,11887,11894,12063,12069,12073,12084,12247,12252,12253,12274,12275,12278,12279,12293,12295,12297,12448,12460,12463,12471,12479,12494,12657,12665,12667,12673,12849,12862,12866,12867,12874,12877,12879,12880,13011,13018,13022,13024,13025,13027,13029,13031,13046,13049,13058,13062,13231,13233,13251,13254,13259,13440,13453,13455,13458,13459,13463,13615,13626,13643,13784,13794,13801,13802,13806,13809,13813,13815,13819,13822,13825,13826,13830,13841,13845,13847,13974,13975,13994,14036,14040,14042,14055,14210,14213,14217,14219,14221,14222,14232,14234,14236,14238,14240,14242,14247,14461,14468,14469,14470,14471,14480,14650,14684,14686,14838,14855,14859,14861,14863,14864,14869,14876,14888,14903,14904,15079,15080,15083,15085,15088,15249,15262,15267,15292,15309,15312,15315,15469,15490,15491,15492,15494,15496,15499,15501,15513,15516,15534,15663,15665,15671,15674,15675,15677,15689,15693,15697,15700,15711,15713,15714,15716,15717,15720,15722,15724,15727,15734,15894,15898,15899,15901,15903,15905,15907,15909,15911,15914,15920,15924,15925,15927,15949,16116,16118,16123,16124,16292,16298,16304,16484,16486,16489,16491,16495,16932,16935,16938,16943,16944,16946,16960,16961,16963,16965,16970,16977,16978,16992,16998,17132,17139,17141,17144,17149,17155,17157,17180,17184,17339,17341,17345,17503,17509,17521,17524,17527,17535,17537,17541,17544,17545,17552,17556,17558,17563,17565,17572,17585, ptg000001l:31406-49213:m54329U_210323_190418/94437983/ccs block 128,0,128,0 Ignored m6A +ptg000001l 31941 48017 5mC 0 - 31941 48017 139,69,19,1 113 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,179,1045,1251,1347,1379,1436,1438,1880,2225,3523,3533,3665,3721,3728,4212,4472,4698,5102,5243,5249,5283,5306,5327,5337,5341,5401,5431,5459,5788,5828,5879,6841,7367,7764,7772,7963,8065,8067,8101,8196,8245,8259,8267,8269,8273,8286,8288,8292,8314,8338,8343,8350,8362,8375,8377,8380,8384,8392,8435,8440,8442,8449,8456,8463,8539,8571,8583,8593,8600,8602,8799,8847,9045,9077,9311,9415,9423,9576,9586,9608,9628,9635,9644,9672,9763,9773,9775,9779,9827,10390,10398,10459,10528,10534,10564,10572,10577,10581,10588,10721,10729,10753,10799,11038,13193,13208,13299,13385,13457,14319,14353,16075, ptg000001l:31406-49213:m54329U_210323_190418/94437983/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 31521 49001 NUC 0 - 31521 49001 169,169,169,1 85 164,164,167,183,97,176,130,319,121,157,154,376,158,191,156,179,156,151,191,204,140,159,161,151,143,115,132,105,157,165,554,124,127,150,150,149,197,162,180,152,183,156,160,141,169,167,160,143,130,173,145,185,175,159,149,156,165,165,168,162,150,162,175,130,168,180,151,140,126,154,213,169,151,174,160,153,128,159,166,167,179,436,133,154,157, 0,169,342,520,746,880,1072,1234,1574,1734,1920,2094,2525,2692,2892,3125,3321,3506,3668,3868,4108,4290,4493,4673,4895,5096,5300,5454,5653,5850,6016,6620,6830,7024,7222,7411,7561,7785,7957,8194,8360,8590,8780,9010,9187,9368,9561,9751,9982,10165,10371,10535,10730,10922,11106,11285,11504,11684,11872,12062,12275,12472,12651,12858,13040,13237,13441,13621,13825,14033,14225,14458,14664,14882,15066,15293,15512,15712,15927,16102,16282,16473,16976,17162,17323, ptg000001l:31406-49213:m54329U_210323_190418/94437983/ccs block 169,169,169,0 Ignored NUC +ptg000001l 41415 41503 FIRE 0 - 41415 41503 255,0,0,1 1 88, 0, ptg000001l:31406-49213:m54329U_210323_190418/94437983/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 31498 49084 LINKER 0 - 31498 49084 147,112,219,1 85 23,5,9,11,43,37,16,32,21,39,29,20,55,9,9,77,17,29,11,9,36,42,44,19,71,58,89,22,94,40,1,50,86,67,48,39,1,27,10,57,14,47,34,70,36,12,26,30,53,33,19,10,17,25,30,63,15,23,22,51,47,17,32,52,29,24,29,64,82,38,20,37,67,10,67,66,72,56,9,13,12,67,53,7,83, 0,187,356,532,726,866,1079,1225,1576,1718,1914,2097,2493,2706,2906,3071,3327,3500,3680,3882,4095,4271,4472,4677,4847,5061,5234,5455,5582,5833,6038,6593,6767,6980,7197,7395,7583,7781,7970,8160,8369,8566,8769,8963,9174,9379,9558,9744,10135,10361,10539,10743,10928,11104,11278,11464,11692,11872,12063,12247,12448,12657,12849,13011,13231,13440,13615,13784,13974,14210,14461,14650,14838,15079,15249,15469,15663,15894,16116,16292,16484,16932,17132,17339,17503, ptg000001l:31406-49213:m54329U_210323_190418/94437983/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 42985 56636 m6A 0 - 42985 56636 128,0,128,1 585 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,400,541,671,680,682,685,688,690,862,864,869,873,876,879,899,902,906,1049,1056,1065,1068,1082,1090,1094,1098,1101,1104,1106,1108,1109,1119,1252,1255,1256,1258,1259,1261,1266,1279,1284,1286,1450,1452,1464,1492,1499,1638,1641,1643,1648,1650,1662,1671,1686,1690,1697,1848,1861,1866,1870,1872,1877,1884,1887,1923,2046,2051,2052,2057,2059,2063,2065,2066,2068,2070,2256,2258,2261,2281,2288,2464,2466,2469,2627,2628,2817,3191,3197,3203,3208,3374,3376,3377,3382,3389,3409,3580,3584,3587,3589,3592,3593,3596,3597,3598,3601,3617,3766,3768,3770,3780,3805,3825,3828,3834,4003,4009,4012,4014,4016,4020,4022,4023,4026,4169,4176,4178,4184,4186,4187,4190,4193,4203,4207,4210,4213,4214,4220,4224,4226,4227,4229,4230,4233,4245,4379,4384,4386,4387,4390,4392,4394,4397,4399,4407,4411,4412,4414,4416,4418,4420,4422,4424,4427,4428,4431,4433,4437,4438,4442,4443,4444,4459,4460,4462,4464,4466,4467,4469,4636,4644,4647,4648,4650,4656,4805,4807,4808,4810,4814,4825,4827,4828,4832,4835,4836,4838,4842,4843,4845,4846,4851,4854,4999,5001,5002,5004,5008,5024,5370,5396,5546,5550,5556,5697,5707,5712,5719,5723,5724,5728,5740,5742,5745,5747,5748,5767,5768,5773,5929,5931,5935,5952,5965,5970,6112,6117,6118,6125,6128,6131,6133,6156,6159,6318,6322,6335,6339,6341,6343,6346,6347,6360,6364,6365,6369,6373,6376,6378,6381,6527,6532,6545,6547,6551,6552,6558,6561,6562,6565,6568,6572,6747,6752,6754,6921,6932,7095,7098,7104,7111,7115,7120,7122,7127,7130,7132,7135,7137,7150,7284,7299,7302,7309,7311,7313,7314,7318,7326,7327,7329,7675,7678,7679,7684,7686,7694,7698,7781,7846,7854,8018,8025,8036,8039,8042,8062,8213,8217,8220,8379,8382,8383,8384,8390,8400,8403,8418,8420,8422,8423,8425,8431,8432,8434,8571,8582,8586,8589,8590,8596,8603,8605,8608,8613,8620,8622,8624,8626,8630,8631,8633,8636,8637,8640,8642,8645,8649,8653,8783,8804,8808,8811,8814,8831,8832,8972,8979,8985,8999,9000,9004,9005,9018,9020,9022,9026,9399,9542,9872,9874,9878,9883,9916,9927,10080,10239,10250,10261,10264,10422,10434,10439,10440,10441,10447,10595,10598,10612,10614,10617,10618,10620,10623,10627,10790,10796,10800,10803,10934,10953,10956,10959,10967,10968,10972,10974,10976,10982,10986,10989,10992,11016,11018,11157,11162,11165,11299,11302,11303,11317,11331,11334,11336,11338,11339,11342,11344,11348,11350,11364,11507,11513,11514,11530,11534,11546,11550,11706,11708,11723,12037,12042,12045,12049,12050,12062,12066,12068,12070,12075,12078,12083,12085,12238,12253,12257,12263,12266,12416,12418,12419,12420,12422,12438,12440,12444,12450,12603,12607,12609,12611,12613,12615,12618,12621,12634,12636,12638,12640,12642,12645,12647,12778,12785,12792,12794,12796,12802,12803,12805,12807,12811,12813,12814,12815,12819,12820,12832,12834,12835,12853,12855,12997,13001,13003,13005,13009,13011,13016,13018,13021,13023,13025,13028,13042,13046,13049,13063,13083,13177,13179,13181,13184,13199,13202,13205,13206,13208,13211,13212,13215,13217,13219,13224,13226,13227,13236,13239,13241,13244,13248,13254,13256,13258,13261,13262,13264,13266,13269,13437,13443,13445,13447,13448,13450,13452,13455,13456,13458,13474,13622,13624,13628,13629,13634,13635,13637,13639,13640,13642,13646,13648,13650, ptg000001l:42836-56647:m54329U_210323_190418/162202206/ccs block 128,0,128,0 Ignored m6A +ptg000001l 43403 56209 5mC 0 - 43403 56209 139,69,19,1 45 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,1143,1731,1746,1837,1877,1923,2562,3851,6479,6519,6569,6580,6609,6613,6621,6625,6684,6701,6731,6852,7332,7448,7466,7553,7604,7617,7744,7772,7782,7784,7788,8713,9136,9335,9339,9463,9729,10338,10358,11013,11991,12050,12714,12805, ptg000001l:42836-56647:m54329U_210323_190418/162202206/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 42986 56422 NUC 0 - 42986 56422 169,169,169,1 64 399,140,129,171,142,132,163,138,150,158,185,175,157,188,373,165,170,148,168,142,133,166,148,144,345,149,140,155,141,158,145,174,166,162,133,345,147,163,150,158,136,129,139,372,142,329,152,158,157,147,162,130,138,133,142,155,313,152,149,152,130,141,93,167, 0,400,541,690,906,1119,1286,1499,1697,1887,2070,2288,2469,2628,2817,3208,3409,3617,3834,4026,4245,4469,4656,4854,5024,5396,5556,5773,5970,6159,6381,6572,6754,6932,7150,7329,7698,7854,8062,8220,8434,8653,8832,9026,9399,9542,9927,10080,10264,10447,10627,10803,11018,11165,11364,11550,11723,12085,12266,12450,12647,12855,13083,13269, ptg000001l:42836-56647:m54329U_210323_190418/162202206/ccs block 169,169,169,0 Ignored NUC +ptg000001l 42985 56460 LINKER 0 - 42985 56460 147,112,219,1 65 1,1,1,20,45,71,35,50,60,40,25,33,6,2,1,18,36,38,69,24,77,91,21,50,26,27,11,77,42,48,64,46,8,12,56,46,24,9,45,8,56,83,50,55,1,1,56,1,26,26,33,14,85,9,66,44,18,49,29,35,45,78,87,93,38, 0,400,541,671,862,1049,1252,1450,1638,1848,2046,2256,2464,2627,2817,3191,3374,3580,3766,4003,4169,4379,4636,4805,4999,5370,5546,5697,5929,6112,6318,6527,6747,6921,7095,7284,7675,7846,8018,8213,8379,8571,8783,8972,9399,9542,9872,10080,10239,10422,10595,10790,10934,11157,11299,11507,11706,12037,12238,12416,12603,12778,12997,13177,13437, ptg000001l:42836-56647:m54329U_210323_190418/162202206/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 51903 71292 m6A 0 - 51903 71292 128,0,128,1 814 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,172,177,178,194,198,366,368,370,555,562,566,721,728,730,733,739,745,913,923,929,1102,1114,1129,1130,1269,1270,1277,1279,1291,1297,1482,1490,1493,1643,1654,1658,1661,1680,1865,1872,1877,1878,2022,2027,2047,2054,2056,2058,2059,2064,2068,2072,2217,2231,2232,2242,2244,2247,2251,2257,2269,2413,2426,2430,2432,2436,2439,2444,2446,2447,2450,2454,2468,2473,2476,2616,2628,2632,2633,2635,2637,2639,2641,2643,2646,2649,2651,2652,2654,2677,2829,2833,2838,2840,2844,2846,2851,3020,3044,3048,3071,3212,3235,3241,3406,3411,3607,3619,3636,3767,3788,3791,3792,3794,3796,3799,3801,3802,3804,3807,3808,3811,3813,3820,3824,4022,4026,4217,4219,4225,4411,4580,4582,4585,4934,4940,4942,4946,4949,4979,4982,4983,4985,4988,4996,5010,5016,5019,5025,5027,5032,5153,5180,5181,5229,5377,5408,5437,5541,5558,5565,5569,5573,5577,5590,5593,5595,5757,5775,5962,6148,6153,6155,6290,6294,6296,6303,6401,6504,6506,6528,6531,6533,6535,6540,6702,6746,6903,6920,6924,6927,6931,6933,6938,6950,7126,7130,7131,7134,7137,7147,7148,7150,7154,7169,7222,7257,7259,7261,7263,7291,7293,7295,7297,7299,7310,7311,7313,7315,7317,7319,7321,7323,7325,7327,7329,7331,7337,7339,7341,7343,7345,7352,7355,7365,7367,7369,7371,7373,7375,7389,7391,7535,7547,7700,7719,7721,7722,7726,7728,7730,7734,7740,7745,7758,7768,7910,7920,7965,8095,8099,8132,8134,8137,8142,8143,8145,8149,8156,8164,8168,8328,8334,8342,8344,8346,8350,8358,8363,8366,8369,8371,8372,8381,8383,8385,8543,8545,8547,8549,8551,8553,8555,8556,8559,8561,8570,8574,8738,8741,8749,8755,8765,8766,8768,8769,8771,8772,8774,8779,8781,8785,8790,8794,8803,8934,8936,8938,8941,8943,8946,8953,8960,8966,8979,8981,8983,8985,9140,9143,9146,9152,9154,9155,9159,9332,9335,9337,9352,9355,9356,9363,9366,9367,9369,9371,9373,9375,9376,9379,9382,9387,9390,9394,9553,9559,9572,9574,9576,9588,9590,9592,9596,9598,9605,9609,9616,9746,9749,9752,9778,9790,9793,9795,9957,9964,9982,9999,10154,10172,10173,10175,10178,10192,10195,10197,10199,10202,10205,10218,10222,10224,10225,10231,10381,10385,10388,10391,10394,10396,10397,10403,10424,10431,10581,10598,10599,10600,10601,10606,10608,10616,10769,10809,10813,10815,10826,10829,10977,10983,10985,10989,10995,10999,11003,11008,11013,11019,11023,11028,11030,11041,11045,11049,11053,11061,11064,11185,11198,11210,11237,11245,11586,11605,11621,11623,11627,11628,11629,11633,11638,11641,11643,11646,11649,11791,11798,11800,11802,11817,11828,11830,11835,11838,11840,11844,11846,11851,11854,11858,11863,11866,11868,11873,11877,11884,11888,11891,12017,12019,12021,12037,12044,12046,12048,12059,12062,12063,12064,12068,12071,12083,12086,12090,12099,12102,12103,12104,12114,12117,12267,12270,12272,12276,12277,12282,12283,12285,12288,12293,12295,12296,12306,12310,12312,12313,12315,12316,12319,12321,12340,12344,12352,12359,12363,12487,12491,12521,12523,12532,12539,12544,12546,12551,12558,12561,12631,12697,12700,12703,12706,12708,12714,12717,12719,12720,12725,12734,12736,12740,12747,12771,12881,12884,12896,12898,12901,12913,12915,12917,12920,12923,12926,12933,12937,12939,12943,12945,12957,12964,12966,12970,12978,12988,13132,13136,13139,13141,13148,13151,13154,13158,13160,13161,13164,13166,13170,13174,13188,13189,13192,13195,13353,13354,13507,13511,13512,13513,13516,13519,13539,13542,13554,13559,13732,13734,13746,13919,13939,13944,13948,13950,13951,13955,13958,13970,13974,13978,14168,14315,14317,14353,14354,14529,14534,14722,14725,14740,14744,14750,14768,14883,14928,15278,15289,15291,15293,15477,15485,15489,15495,15497,15503,15657,15693,15695,15700,15703,15708,15714,15717,15834,15839,15855,15857,15861,15964,16052,16054,16058,16066,16069,16073,16075,16076,16078,16082,16089,16093,16097,16217,16228,16247,16249,16253,16257,16261,16278,16282,16443,16457,16458,16461,16463,16464,16473,16499,16622,16640,16646,16649,16657,16659,16665,16670,16838,16842,16845,16846,16851,16855,16857,16859,16861,16863,16865,16867,16869,16871,16873,16883,16885,16889,16891,17029,17035,17042,17045,17048,17052,17055,17059,17071,17082,17213,17219,17229,17232,17234,17236,17237,17264,17267,17407,17415,17416,17421,17430,17434,17437,17445,17450,17453,17456,17477,17480,17618,17628,17633,17640,17643,17644,17652,17659,17661,17664,17667,17831,17833,17834,17837,17840,17842,17845,17855,17857,17862,17864,17866,17873,17879,18030,18036,18040,18054,18057,18061,18066,18071,18243,18245,18627,18647,18798,18804,18816,18818,18821,18822,18825,18833,18974,18994,18996,18999,19012,19016,19019,19022,19027,19030,19035,19041,19178,19184,19192,19196,19200,19203,19207,19213,19216,19388, ptg000001l:51835-71565:m54329U_210323_190418/143787994/ccs block 128,0,128,0 Ignored m6A +ptg000001l 52539 71409 5mC 0 - 52539 71409 139,69,19,1 76 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,81,199,203,242,262,280,289,317,593,1202,1222,1877,2314,2820,2855,2936,3419,3699,4547,4603,4627,4657,4667,4722,4840,5531,5568,5579,6357,6413,6550,8078,8123,8148,8219,8223,8522,8991,10381,10509,10966,11513,11520,11565,11574,11994,12123,12341,12430,12435,13815,14062,14259,15580,15916,16580,16582,16614,16922,16955,17309,17406,17927,17930,18093,18136,18165,18227,18559,18601,18676,18736,18738,18746,18869, ptg000001l:51835-71565:m54329U_210323_190418/143787994/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 51904 71291 NUC 0 - 51904 71291 169,169,169,1 96 171,167,184,154,167,172,138,184,149,184,143,144,143,139,151,168,140,164,195,130,197,190,185,168,348,147,147,132,161,186,185,134,97,102,161,156,175,102,143,152,141,129,159,157,163,130,154,172,158,129,161,154,149,149,152,147,120,340,141,125,149,123,135,109,143,157,152,172,172,189,146,174,187,159,349,183,153,116,102,87,119,160,148,167,137,130,139,137,163,150,171,381,150,140,136,171, 0,198,370,566,745,929,1130,1297,1493,1680,1878,2072,2269,2476,2677,2851,3071,3241,3411,3636,3824,4026,4225,4411,4585,5032,5229,5408,5595,5775,5962,6155,6303,6401,6540,6746,6950,7154,7391,7547,7768,7965,8168,8385,8574,8803,8985,9159,9394,9616,9795,9999,10231,10431,10616,10829,11064,11245,11649,11891,12117,12363,12561,12771,12988,13195,13354,13559,13746,13978,14168,14354,14534,14768,14928,15293,15503,15717,15861,15964,16097,16282,16473,16670,16891,17082,17267,17480,17667,17879,18071,18245,18647,18833,19041,19216, ptg000001l:51835-71565:m54329U_210323_190418/143787994/ccs block 169,169,169,0 Ignored NUC +ptg000001l 59160 59295 FIRE 0 - 59160 59295 225,0,0,1 1 135, 0, ptg000001l:51835-71565:m54329U_210323_190418/143787994/ccs block 225,0,0,0 Ignored FIRE +ptg000001l 51903 71292 LINKER 0 - 51903 71292 147,112,219,1 96 1,27,5,12,25,17,29,29,12,38,14,51,53,64,62,23,52,30,6,30,58,5,9,1,6,99,50,32,55,19,1,8,14,1,37,45,48,29,13,69,56,74,58,32,66,52,20,63,64,50,43,78,51,36,61,88,61,64,101,101,97,75,75,108,64,2,53,15,60,1,40,6,47,1,16,27,61,28,1,46,66,31,49,54,54,55,74,50,49,42,3,21,36,68,39,1, 0,172,366,555,721,913,1102,1269,1482,1643,1865,2022,2217,2413,2616,2829,3020,3212,3406,3607,3767,4022,4217,4411,4580,4934,5180,5377,5541,5757,5962,6148,6290,6401,6504,6702,6903,7126,7535,7700,7910,8095,8328,8543,8738,8934,9140,9332,9553,9746,9957,10154,10381,10581,10769,10977,11185,11586,11791,12017,12267,12487,12697,12881,13132,13353,13507,13732,13919,14168,14315,14529,14722,14928,15278,15477,15657,15834,15964,16052,16217,16443,16622,16838,17029,17213,17407,17618,17831,18030,18243,18627,18798,18974,19178,19388, ptg000001l:51835-71565:m54329U_210323_190418/143787994/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 52179 71619 m6A 0 + 52179 71619 128,0,128,1 1371 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,145,152,159,162,165,332,348,501,510,517,647,661,664,668,678,680,684,834,838,840,846,847,849,853,854,856,862,866,869,872,874,876,877,879,882,884,886,890,905,1045,1070,1208,1214,1217,1228,1230,1235,1237,1238,1240,1245,1249,1251,1429,1433,1437,1450,1580,1596,1601,1602,1606,1608,1609,1611,1616,1778,1780,1795,1798,1806,1807,1829,1973,1981,1984,1993,2000,2017,2140,2144,2150,2156,2160,2170,2171,2174,2178,2183,2186,2192,2200,2352,2361,2363,2365,2367,2370,2374,2378,2514,2528,2529,2535,2556,2562,2566,2568,2570,2581,2583,2728,2730,2732,2744,2752,2903,2912,2917,2922,2936,3079,3084,3086,3091,3095,3096,3099,3103,3106,3108,3109,3110,3111,3112,3117,3122,3126,3127,3129,3130,3133,3141,3143,3148,3275,3276,3297,3302,3303,3306,3308,3457,3471,3477,3484,3491,3496,3505,3515,3661,3667,3673,3674,3680,3826,3829,3831,3834,3842,3855,3859,3861,3864,3866,3869,3871,3877,3880,3882,3889,3912,4030,4047,4203,4215,4218,4219,4222,4223,4225,4228,4389,4394,4403,4411,4413,4560,4563,4580,4610,4612,4614,4618,4621,4629,4632,4731,4734,4740,4743,4747,4749,4756,4759,4764,4767,4770,4773,4777,4779,4780,4782,4784,4785,4787,4790,4793,4796,4798,4799,4800,4803,4805,4807,4812,4818,4819,4820,4822,4824,4827,4830,4832,4834,4836,4839,4845,4943,4949,4953,4955,4957,4959,4967,4971,4975,4976,4979,4981,4984,4988,4989,4991,4993,4996,4998,5000,5004,5007,5013,5019,5023,5024,5028,5029,5030,5032,5035,5041,5138,5160,5161,5163,5165,5167,5170,5171,5176,5179,5180,5181,5183,5184,5185,5187,5188,5190,5191,5193,5195,5196,5198,5201,5202,5203,5206,5208,5213,5215,5216,5217,5220,5222,5225,5226,5229,5230,5231,5233,5235,5236,5239,5243,5246,5248,5250,5251,5253,5255,5257,5261,5265,5266,5268,5269,5271,5273,5276,5277,5289,5408,5410,5412,5413,5433,5438,5441,5442,5444,5445,5446,5448,5450,5451,5455,5458,5459,5462,5465,5467,5469,5470,5475,5476,5481,5614,5627,5647,5782,5784,5786,5789,5791,5796,5798,5803,5804,5805,5806,5809,5812,5835,5837,5838,5839,5961,5967,5975,6004,6012,6134,6144,6150,6155,6159,6161,6164,6168,6170,6174,6176,6180,6182,6195,6202,6207,6357,6359,6360,6363,6373,6390,6541,6562,6567,6568,6571,6593,6720,6727,6741,6744,6752,6756,6758,6766,6768,6769,6771,6774,6777,6793,6889,6895,6899,6902,6904,6905,6917,6921,6925,6927,6930,6932,6934,6936,6938,6942,6943,6945,6946,6948,6950,6953,6955,6958,6960,6967,6985,6987,7093,7095,7097,7099,7101,7102,7106,7108,7130,7132,7162,7304,7307,7310,7311,7314,7315,7316,7318,7322,7325,7474,7492,7494,7510,7513,7514,7521,7525,7527,7530,7534,7542,7545,7683,7685,7689,7691,7699,7702,7719,7727,7730,7732,7739,7741,7744,7746,7747,7793,7890,7897,7899,7906,7907,7908,7910,7912,7914,7915,7917,7926,7929,7935,7937,7939,8093,8096,8105,8107,8137,8138,8255,8262,8264,8267,8269,8273,8275,8277,8279,8280,8283,8285,8288,8294,8295,8298,8301,8302,8306,8312,8315,8317,8319,8335,8337,8339,8343,8489,8495,8496,8503,8509,8511,8514,8518,8520,8527,8530,8540,8663,8675,8677,8681,8684,8695,8697,8699,8701,8703,8707,8709,8727,8876,8879,8884,8887,9064,9068,9087,9090,9091,9093,9100,9103,9104,9106,9110,9111,9114,9116,9131,9251,9257,9259,9264,9266,9271,9277,9278,9280,9283,9284,9292,9294,9296,9297,9300,9312,9314,9334,9470,9473,9476,9478,9492,9495,9496,9498,9514,9517,9667,9672,9674,9680,9684,9686,9701,9705,9706,9862,9863,9865,9869,9878,9894,9896,9897,9899,9901,9902,9903,9909,9911,9912,9916,9919,9921,10059,10089,10090,10094,10095,10097,10102,10109,10115,10118,10120,10121,10222,10250,10251,10254,10256,10259,10261,10263,10268,10270,10273,10285,10288,10290,10291,10294,10299,10301,10302,10305,10306,10309,10311,10314,10318,10322,10340,10448,10457,10463,10468,10471,10472,10477,10478,10483,10484,10487,10490,10493,10495,10497,10502,10507,10511,10513,10516,10518,10528,10529,10533,10535,10537,10539,10547,10550,10553,10556,10558,10559,10699,10701,10703,10704,10707,10709,10711,10713,10719,10723,10727,10730,10732,10737,10743,10745,10747,10748,10752,10756,10763,10765,10880,10889,10893,10896,10901,10909,10912,10914,10915,10920,10922,10927,10932,10934,10936,11104,11262,11263,11267,11268,11270,11275,11281,11286,11291,11292,11294,11296,11298,11300,11301,11304,11305,11306,11309,11310,11312,11315,11316,11317,11324,11329,11331,11334,11339,11342,11345,11347,11348,11351,11352,11353,11362,11502,11506,11507,11511,11515,11516,11520,11522,11524,11526,11528,11532,11535,11536,11537,11539,11541,11543,11544,11548,11552,11554,11568,11570,11581,11587,11638,11687,11700,11705,11708,11710,11711,11712,11714,11715,11717,11718,11721,11727,11731,11733,11734,11738,11740,11741,11743,11745,11761,11768,11892,11905,11907,11914,11916,11919,11921,11929,11931,11932,11937,11942,11944,12094,12104,12106,12107,12108,12113,12115,12117,12118,12121,12122,12126,12137,12139,12141,12144,12150,12164,12168,12169,12319,12326,12327,12329,12333,12336,12337,12346,12347,12351,12355,12356,12369,12373,12376,12542,12544,12550,12556,12564,12570,12694,12702,12707,12709,12712,12713,12726,12729,12731,12733,12735,12737,12738,12747,12750,12754,12810,12812,12856,12860,12861,12863,12865,12872,12875,12877,12878,12880,12882,12885,12888,12890,12893,12894,12898,12900,12902,12904,12906,12908,12909,12912,12913,12916,12919,12924,12926,12927,12930,12931,12932,12936,12937,12942,12944,12954,12956,12957,12965,12969,12970,12973,12978,12981,12987,12990,12993,13118,13121,13128,13130,13133,13135,13137,13140,13142,13145,13148,13153,13175,13179,13180,13305,13308,13313,13320,13327,13347,13357,13370,13508,13513,13540,13702,13706,13712,13722,13726,13727,13730,13744,13746,13924,13932,14033,14089,14109,14116,14117,14119,14124,14125,14129,14134,14138,14143,14148,14310,14317,14319,14321,14323,14324,14326,14327,14328,14330,14332,14346,14348,14350,14423,14480,14484,14487,14488,14490,14495,14508,14509,14517,14522,14560,14681,14692,14694,14700,14705,14710,14714,14719,14722,14833,14835,14881,14888,14894,14896,14898,14900,14911,14918,15047,15050,15052,15055,15058,15060,15062,15065,15066,15068,15070,15072,15074,15077,15078,15079,15081,15086,15090,15092,15100,15103,15104,15106,15111,15112,15116,15120,15122,15125,15128,15132,15273,15279,15281,15282,15284,15286,15288,15310,15313,15455,15458,15481,15485,15486,15490,15507,15686,15690,15691,15694,15696,15698,15708,15847,15859,16010,16013,16017,16021,16028,16033,16035,16039,16044,16197,16204,16209,16211,16221,16223,16228,16237,16240,16242,16245,16252,16253,16255,16258,16263,16267,16277,16279,16419,16421,16425,16428,16436,16438,16440,16442,16444,16446,16451,16609,16611,16613,16615,16617,16619,16621,16623,16625,16627,16629,16631,16633,16635,16637,16639,16642,16643,16645,16657,16660,16661,16663,16665,16667,16669,16671,16806,16808,16812,16815,16816,16817,16819,16826,16828,16833,16839,16846,16847,16850,16852,16991,16996,16998,17003,17010,17013,17015,17018,17020,17021,17024,17027,17028,17034,17195,17197,17199,17201,17203,17204,17207,17215,17218,17219,17222,17223,17228,17232,17241,17266,17269,17271,17278,17283,17388,17391,17393,17395,17397,17406,17425,17427,17431,17442,17443,17446,17594,17599,17619,17622,17625,17629,17635,17785,17788,17793,17795,17798,17800,17804,17806,17810,17922,17954,17955,17957,17964,17967,17969,17973,17984,17986,17991,17993,18000,18008,18010,18018,18022,18023,18027,18141,18214,18293,18299,18302,18303,18306,18332,18334,18336,18338,18344,18346,18485,18494,18499,18504,18506,18513,18526,18528,18531,18682,18686,18688,18696,18698,18702,18706,18720,18845,18859,18863,18866,18868,18871,18873,18876,18883,18885,18890,18892,18899,18905,19027,19030,19034,19037,19040,19042,19047,19050,19053,19056,19216,19220,19223,19225,19230,19322,19380,19382,19385,19388,19395,19397,19400,19401,19402,19405,19407,19408,19412,19415,19423,19426,19427,19439, ptg000001l:52150-71729:m54329U_210323_190418/98239231/ccs block 128,0,128,0 Ignored m6A +ptg000001l 52619 71634 5mC 0 + 52619 71634 139,69,19,1 85 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,30,47,54,110,150,161,208,215,246,252,1141,1200,1796,2774,3378,3497,4228,4546,4576,4586,4628,4641,5498,5525,6276,6321,6346,6835,7008,7997,7999,8042,8046,8441,8910,10074,10216,10300,10428,10459,10535,11318,11432,11439,11484,11486,11493,11947,12260,12265,12349,12354,12383,12413,14178,15283,15306,15410,15462,15466,15499,15519,15835,16423,16499,16501,16738,16823,16874,16937,17418,17846,17849,17874,18055,18084,18478,18520,18655,18665,18969,18980,19012,19014, ptg000001l:52150-71729:m54329U_210323_190418/98239231/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 52345 71559 NUC 0 + 52345 71559 169,169,169,1 100 166,152,129,149,139,137,177,129,161,143,122,151,135,144,150,142,126,148,145,145,117,155,160,146,98,97,96,118,132,134,121,121,149,150,126,95,105,141,148,137,142,153,116,145,122,148,176,119,135,149,155,137,128,107,139,114,167,157,139,105,123,149,149,165,123,101,124,124,137,161,177,156,161,129,158,110,128,140,141,178,138,150,152,139,157,134,138,160,104,147,149,143,186,78,138,150,124,121,159,149, 0,183,352,519,740,905,1086,1285,1451,1664,1852,2035,2213,2418,2587,2771,2983,3143,3350,3515,3747,3882,4063,4248,4467,4680,4876,5124,5316,5482,5674,5847,6042,6225,6428,6628,6822,6997,7160,7380,7582,7774,7973,8178,8375,8562,8722,8966,9169,9352,9541,9756,9956,10175,10394,10600,10771,10939,11197,11416,11603,11779,12004,12211,12405,12589,12828,13015,13205,13375,13581,13767,13983,14185,14357,14557,14753,14967,15148,15342,15543,15694,15879,16114,16286,16506,16687,16869,17118,17281,17470,17645,17862,18049,18181,18366,18555,18740,18891,19065, ptg000001l:52150-71729:m54329U_210323_190418/98239231/ccs block 169,169,169,0 Ignored NUC +ptg000001l 65035 65173 FIRE 0 + 65035 65173 225,0,0,1 1 138, 0, ptg000001l:52150-71729:m54329U_210323_190418/98239231/ccs block 225,0,0,0 Ignored FIRE +ptg000001l 57317 63542 FIRE 0 + 57317 63542 255,0,0,1 2 152,101, 0,6124, ptg000001l:52150-71729:m54329U_210323_190418/98239231/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 52324 71619 LINKER 0 + 52324 71619 147,112,219,1 98 21,17,17,38,72,26,44,22,37,52,45,61,27,70,25,34,70,34,59,20,87,18,26,25,73,115,99,74,34,58,52,74,34,53,74,99,70,22,72,65,50,46,89,52,65,12,68,84,48,40,60,63,91,112,67,57,1,80,82,53,76,58,29,61,63,66,33,45,9,60,41,43,42,86,86,41,53,23,13,35,83,33,63,47,44,89,59,42,26,74,1,54,47,39,61,30,15,60, 0,187,356,502,689,900,1063,1284,1435,1633,1828,1995,2207,2369,2583,2758,2934,3130,3312,3516,3681,3885,4058,4244,4415,4586,4798,5263,5469,5637,5816,5989,6212,6396,6575,6744,6948,7159,7329,7538,7745,7948,8110,8344,8518,8731,8919,9106,9325,9522,9717,9914,10105,10303,10554,10735,10959,11357,11542,11747,11949,12174,12397,12549,12973,13160,13363,13557,13779,13944,14165,14335,14536,14688,14902,15128,15310,15541,15702,15865,16052,16274,16464,16661,16846,17050,17243,17449,17640,17809,18069,18148,18340,18537,18700,18882,19071,19235, ptg000001l:52150-71729:m54329U_210323_190418/98239231/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 56422 72931 m6A 0 - 56422 72931 128,0,128,1 1833 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,2,6,11,18,21,40,42,50,54,186,187,188,191,192,193,197,198,199,200,203,205,207,209,211,212,213,215,221,222,224,225,226,229,231,232,235,237,240,242,244,247,248,250,253,254,256,258,259,261,263,264,267,268,271,278,386,402,403,408,410,412,413,415,417,419,420,421,423,427,430,433,434,436,443,447,450,452,460,463,464,466,469,539,587,589,591,593,596,598,601,607,612,615,617,618,620,621,622,626,628,629,630,634,635,636,637,639,642,643,645,661,776,785,786,789,792,796,798,802,807,811,917,918,920,933,936,937,938,944,947,948,950,952,953,955,959,960,963,965,970,972,986,988,992,1003,1005,1007,1008,1012,1018,1023,1028,1065,1190,1195,1198,1199,1201,1202,1205,1207,1208,1212,1215,1216,1219,1222,1224,1343,1345,1351,1353,1355,1357,1363,1371,1388,1390,1537,1539,1541,1543,1546,1688,1698,1701,1704,1708,1710,1713,1715,1719,1721,1724,1726,1732,1874,1878,1882,1894,1896,1897,1901,1907,1914,1927,2053,2056,2067,2070,2075,2077,2079,2081,2117,2258,2260,2262,2264,2265,2267,2272,2478,2482,2484,2498,2501,2509,2513,2515,2523,2525,2526,2528,2531,2534,2536,2538,2539,2541,2542,2545,2547,2550,2551,2554,2567,2674,2693,2810,2812,2815,2817,2819,2820,2822,2824,2826,2834,2836,2838,2840,2842,2844,2847,2848,2850,2852,2854,2856,2865,2866,2868,2870,2872,2874,2876,2883,2885,2887,2889,2891,2893,2895,2896,2900,2902,2905,2907,2911,2912,2935,3010,3019,3022,3026,3027,3028,3031,3034,3039,3052,3054,3056,3061,3064,3067,3069,3073,3075,3076,3079,3082,3202,3209,3211,3354,3370,3377,3381,3383,3385,3387,3390,3391,3394,3397,3398,3399,3401,3403,3408,3414,3421,3423,3429,3435,3436,3440,3442,3446,3558,3582,3585,3587,3590,3593,3595,3598,3599,3601,3603,3605,3608,3609,3615,3620,3623,3750,3762,3764,3767,3769,3778,3784,3785,3799,3926,3942,3948,3951,3955,3958,3959,3960,3963,3966,3984,4100,4121,4126,4128,4132,4266,4268,4271,4283,4297,4299,4310,4311,4323,4329,4332,4348,4466,4492,4503,4507,4511,4515,4542,4548,4549,4567,4568,4572,4587,4702,4704,4707,4709,4711,4724,4727,4730,4742,4850,4868,4871,4873,4875,4888,4890,4898,4904,4905,4907,4915,4923,4927,4930,4940,4944,4946,4948,4952,5053,5073,5077,5086,5091,5097,5101,5104,5109,5113,5115,5118,5120,5129,5132,5135,5136,5139,5142,5271,5274,5276,5294,5295,5298,5299,5301,5302,5303,5306,5308,5310,5311,5312,5313,5316,5318,5319,5320,5323,5325,5326,5335,5338,5342,5343,5345,5346,5348,5350,5353,5355,5358,5480,5492,5506,5507,5513,5515,5516,5519,5520,5525,5527,5529,5534,5540,5542,5543,5545,5548,5549,5550,5553,5557,5563,5565,5568,5573,5584,5585,5590,5710,5728,5735,5742,5748,5749,5759,5762,5764,6058,6059,6062,6072,6074,6075,6078,6079,6082,6083,6087,6094,6097,6201,6206,6214,6217,6220,6225,6228,6234,6235,6240,6241,6244,6247,6250,6252,6254,6259,6264,6268,6270,6273,6275,6278,6285,6286,6290,6294,6296,6304,6307,6310,6313,6456,6458,6460,6461,6464,6466,6468,6470,6471,6472,6476,6480,6484,6487,6500,6502,6504,6505,6509,6511,6513,6514,6516,6520,6522,6526,6619,6632,6634,6636,6637,6638,6640,6646,6650,6653,6654,6655,6658,6659,6661,6664,6666,6669,6671,6672,6675,6676,6677,6679,6684,6689,6691,6693,6696,6699,6701,6703,6704,6708,6709,6711,6713,6717,6720,6724,6728,6737,6739,6741,6742,6743,6745,6750,6876,6885,6891,6893,6895,6896,6898,6899,6900,6902,6903,6906,7005,7048,7049,7051,7053,7057,7061,7064,7066,7067,7069,7072,7073,7074,7081,7088,7091,7096,7102,7104,7105,7108,7109,7110,7114,7118,7119,7122,7124,7127,7129,7130,7132,7150,7264,7268,7272,7273,7277,7279,7281,7283,7285,7289,7292,7296,7298,7300,7301,7305,7309,7311,7313,7316,7321,7338,7347,7444,7453,7465,7467,7469,7472,7474,7555,7580,7585,7592,7595,7598,7601,7605,7607,7609,7611,7613,7615,7616,7619,7621,7622,7624,7626,7628,7632,7638,7641,7643,7644,7645,7649,7650,7653,7656,7657,7658,7659,7662,7664,7671,7673,7676,7678,7686,7688,7689,7692,7693,7694,7696,7697,7699,7701,7703,7704,7706,7707,7708,7709,7710,7717,7723,7730,7856,7870,7874,7875,7878,7879,7881,7883,7893,7896,7897,7898,7900,7901,7903,7904,7905,7907,7908,7912,7914,7919,7921,7922,7925,7926,7930,7933,8059,8061,8063,8065,8076,8084,8086,8087,8088,8092,8096,8098,8101,8103,8104,8108,8112,8113,8116,8120,8126,8129,8130,8133,8250,8268,8271,8272,8273,8277,8279,8281,8291,8292,8293,8295,8297,8298,8299,8301,8302,8303,8307,8309,8310,8313,8314,8316,8317,8318,8320,8321,8327,8330,8332,8333,8335,8337,8339,8341,8344,8346,8347,8495,8499,8507,8511,8514,8516,8518,8521,8522,8528,8531,8535,8536,8539,8541,8543,8545,8548,8549,8553,8554,8556,8558,8565,8567,8569,8578,8584,8587,8673,8683,8687,8691,8693,8711,8713,8714,8726,8727,8854,8857,8869,8871,8873,8875,8878,8880,8882,8885,8887,8888,8890,8892,8894,8895,8896,8899,8902,8923,8926,9061,9063,9065,9067,9071,9073,9077,9079,9081,9082,9084,9086,9104,9106,9113,9114,9116,9127,9256,9258,9297,9299,9448,9451,9455,9459,9463,9467,9471,9473,9475,9484,9487,9577,9638,9641,9649,9657,9730,9802,9805,9808,9812,9813,9817,9818,9820,9823,9824,9826,9829,9832,9835,9837,9839,9843,9846,9958,9961,9963,9974,9976,9978,9980,9981,9984,9985,9988,9996,9998,10001,10005,10006,10008,10010,10011,10015,10019,10021,10023,10026,10028,10041,10049,10052,10061,10067,10074,10194,10196,10203,10206,10208,10209,10211,10214,10215,10220,10221,10223,10225,10230,10231,10234,10235,10237,10239,10241,10244,10245,10247,10249,10252,10254,10258,10260,10274,10276,10279,10404,10409,10410,10413,10418,10421,10423,10426,10431,10434,10437,10438,10444,10447,10449,10474,10479,10590,10592,10595,10597,10598,10604,10619,10742,10748,10750,10754,10758,10766,10767,10770,10772,10774,10779,10780,10781,10783,10786,10790,10797,10802,10804,10807,10809,10811,10812,10815,10817,10822,10823,10831,10834,10835,10836,10940,10943,10945,10947,10950,10952,10958,10961,10962,10964,10966,10968,10970,10972,10976,10978,10980,10990,10992,11087,11098,11111,11113,11114,11117,11119,11132,11135,11138,11140,11147,11149,11150,11151,11152,11156,11158,11159,11160,11163,11165,11167,11168,11170,11174,11176,11181,11182,11188,11189,11191,11195,11198,11199,11335,11336,11338,11342,11347,11348,11352,11356,11357,11360,11363,11365,11366,11368,11388,11492,11497,11498,11501,11507,11514,11526,11529,11533,11535,11539,11544,11546,11547,11550,11554,11557,11559,11563,11567,11570,11574,11704,11709,11713,11718,11720,11721,11722,11723,11726,11728,11729,11730,11734,11738,11742,11743,11745,11757,11759,11763,11899,11901,11924,11926,11929,11930,11936,11938,11942,11944,11962,12091,12094,12095,12099,12100,12103,12106,12108,12110,12112,12114,12116,12118,12119,12120,12121,12125,12127,12128,12130,12133,12137,12140,12146,12151,12418,12420,12424,12426,12435,12436,12438,12443,12446,12450,12454,12456,12457,12460,12463,12464,12468,12469,12471,12474,12476,12477,12480,12481,12485,12487,12488,12493,12508,12613,12622,12630,12634,12636,12637,12639,12641,12642,12645,12648,12649,12652,12653,12654,12656,12659,12661,12662,12663,12665,12667,12668,12670,12673,12677,12679,12683,12684,12686,12688,12690,12694,12700,12701,12706,12710,12713,12715,12717,12718,12719,12722,12724,12725,12729,12846,12855,12859,12867,12870,12872,12874,12878,12881,12883,12885,12887,12889,12893,12894,12896,12897,12900,12902,12915,12916,12918,12958,13037,13057,13060,13062,13064,13065,13066,13069,13076,13078,13079,13080,13082,13085,13087,13088,13091,13096,13097,13099,13106,13109,13115,13116,13118,13121,13124,13125,13251,13284,13286,13290,13292,13294,13297,13420,13423,13427,13432,13453,13454,13456,13459,13464,13465,13466,13467,13471,13472,13474,13477,13480,13484,13487,13489,13493,13495,13498,13503,13508,13511,13513,13517,13521,13638,13642,13648,13650,13653,13655,13657,13658,13660,13663,13665,13669,13671,13686,13688,13689,13691,13694,13702,13706,13707,13839,13845,13848,13850,13851,13853,13854,13855,13856,13857,13860,13861,13862,13865,13866,13869,13870,13872,13876,13878,13879,13880,13884,13885,13889,13890,13892,13895,13898,13900,13908,13912,13989,13992,13994,13998,14013,14018,14019,14021,14023,14025,14027,14032,14033,14038,14041,14048,14051,14052,14054,14056,14059,14060,14063,14064,14067,14069,14073,14178,14183,14185,14190,14191,14192,14195,14198,14199,14201,14203,14206,14208,14212,14215,14217,14219,14223,14236,14238,14239,14240,14242,14244,14248,14251,14256,14261,14263,14279,14358,14377,14379,14382,14385,14393,14394,14398,14399,14405,14407,14409,14411,14414,14416,14418,14422,14427,14428,14433,14434,14439,14441,14443,14445,14446,14448,14449,14451,14453,14463,14592,14594,14596,14598,14600,14602,14604,14606,14608,14610,14612,14616,14620,14623,14625,14628,14630,14633,14638,14640,14642,14647,14649,14651,14652,14653,14656,14659,14662,14665,14669,14673,14681,14684,14688,14794,14796,14797,14799,14801,14802,14804,14807,14810,14812,14813,14816,14820,14825,14829,14831,14835,14837,14840,14844,14845,14856,14869,14982,14987,14994,14995,15000,15001,15004,15008,15010,15011,15015,15017,15020,15021,15022,15027,15029,15031,15033,15035,15049,15050,15052,15060,15142,15164,15165,15169,15172,15180,15182,15183,15184,15187,15188,15191,15194,15196,15201,15216,15220,15224,15227,15232,15324,15344,15346,15349,15351,15354,15355,15357,15359,15360,15361,15364,15367,15369,15370,15371,15374,15375,15378,15380,15382,15384,15387,15389,15391,15393,15395,15396,15411,15537,15539,15542,15544,15547,15548,15554,15555,15558,15560,15561,15564,15565,15566,15568,15571,15576,15580,15581,15585,15586,15588,15590,15593,15596,15612,15712,15731,15733,15734,15736,15741,15742,15744,15746,15748,15749,15751,15754,15755,15756,15759,15762,15765,15767,15771,15776,15778,15779,15780,15783,15784,15786,15787,15789,15793,15794,15795,15796,15798,15800,15802,15808,15810,15816,15927,15950,15951,15958,15962,15963,15964,15969,15970,15972,15974,15978,15981,15990,15992,15996,15999,16002,16004,16006,16008,16010,16011,16017,16023,16130,16133,16136,16137,16140,16141,16144,16147,16148,16151,16152,16153,16154,16155,16157,16159,16165,16167,16169,16170,16174,16176,16178,16180,16183,16186,16188,16189,16192,16197,16198,16202,16204,16206,16211,16212,16215,16216,16218,16219,16224,16227,16228,16229,16233,16238,16239,16240,16241,16244,16372,16375,16485,16508, ptg000001l:56406-72943:m54329U_210323_190418/54199156/ccs block 128,0,128,0 Ignored m6A +ptg000001l 56848 72384 5mC 0 - 56848 72384 139,69,19,1 63 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,19,238,318,341,348,358,413,531,1270,2048,2241,2607,3206,3218,3769,3771,3910,3914,4682,6200,7121,7204,7211,7222,7256,7258,7685,7900,8032,8077,8121,8126,8155,8185,8811,9234,11078,11085,11193,11271,12271,12273,12510,12595,12646,13388,13548,13618,13621,13856,14250,14306,14427,14429,14437,14560,14741,14752,14773,14784,14786,15535, ptg000001l:56406-72943:m54329U_210323_190418/54199156/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 56477 72794 NUC 0 - 56477 72794 169,169,169,1 83 131,107,117,114,105,161,118,146,141,141,125,140,205,106,116,106,119,142,111,126,126,115,133,143,114,107,100,128,121,119,293,103,142,92,125,141,113,96,80,125,125,116,147,85,126,134,128,148,150,144,111,119,124,110,122,103,94,135,103,129,135,128,266,104,116,118,158,122,116,131,76,104,78,128,105,112,81,91,125,99,110,106,127, 0,224,415,607,757,974,1170,1336,1492,1678,1873,2063,2218,2513,2639,2858,3028,3157,3392,3569,3745,3930,4078,4294,4533,4688,4898,5088,5304,5536,5710,6043,6259,6472,6696,6852,7096,7293,7420,7676,7879,8079,8293,8533,8673,8872,9073,9245,9433,9603,9792,10020,10225,10425,10565,10782,10938,11145,11334,11520,11709,11908,12097,12454,12675,12864,13071,13243,13467,13653,13858,14019,14225,14409,14634,14815,15006,15178,15357,15558,15762,15969,16190, ptg000001l:56406-72943:m54329U_210323_190418/54199156/ccs block 169,169,169,0 Ignored NUC +ptg000001l 63977 64153 FIRE 0 - 63977 64153 255,0,0,1 1 176, 0, ptg000001l:56406-72943:m54329U_210323_190418/54199156/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 56608 72798 LINKER 0 - 56608 72798 147,112,219,1 82 93,84,75,36,112,35,48,10,45,54,65,15,90,20,103,64,10,93,66,50,59,33,83,96,41,103,90,88,111,55,40,113,71,132,31,103,84,31,78,75,98,93,55,73,67,44,40,20,45,117,86,76,30,95,53,113,54,83,60,64,61,91,117,73,89,14,102,70,74,85,102,106,97,76,79,91,88,76,105,97,115,4, 0,200,401,590,731,1004,1157,1351,1502,1688,1867,2072,2292,2488,2624,2833,3016,3168,3372,3564,3740,3914,4080,4306,4516,4664,4867,5085,5294,5524,5872,6015,6270,6433,6690,6862,7078,7258,7670,7873,8064,8309,8487,8668,8875,9070,9262,9452,9616,9772,10008,10218,10404,10556,10754,10901,11149,11306,11518,11713,11905,12232,12427,12660,12851,13098,13234,13452,13653,13803,13992,14172,14406,14608,14796,14956,15138,15351,15526,15741,15944,16186, ptg000001l:56406-72943:m54329U_210323_190418/54199156/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 58022 71948 m6A 0 + 58022 71948 128,0,128,1 1545 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,150,151,154,156,157,159,161,163,166,169,171,175,177,294,334,337,339,341,342,365,370,372,374,453,456,494,624,641,643,651,653,656,658,660,662,665,667,670,672,675,681,682,685,688,689,691,705,709,710,713,715,717,719,724,725,726,728,731,733,734,735,738,740,742,744,747,748,750,751,756,759,760,765,769,773,775,778,779,781,783,784,785,790,793,798,803,898,901,909,915,923,925,926,928,931,934,938,939,941,942,945,947,950,954,958,960,962,964,967,971,979,1084,1095,1099,1100,1102,1105,1107,1110,1112,1115,1117,1120,1124,1130,1132,1134,1138,1140,1142,1144,1146,1148,1268,1270,1272,1274,1279,1283,1285,1287,1289,1291,1293,1415,1418,1422,1426,1428,1441,1452,1453,1456,1464,1472,1476,1479,1482,1523,1600,1603,1607,1609,1611,1613,1621,1781,1785,1791,1792,1794,1941,1943,1956,1958,1962,1963,1964,1967,1968,1972,1976,1978,1983,1985,1987,1990,1993,1995,1997,1998,2001,2005,2011,2015,2021,2023,2024,2026,2029,2030,2037,2040,2042,2049,2054,2064,2225,2323,2341,2342,2343,2346,2347,2348,2350,2355,2356,2363,2370,2475,2486,2488,2490,2517,2519,2521,2522,2525,2528,2531,2532,2534,2535,2536,2537,2538,2540,2666,2668,2671,2675,2677,2680,2681,2683,2697,2699,2701,2711,2718,2723,2725,2728,2738,2743,2745,2747,2748,2752,2755,2758,2760,2773,2897,2900,2903,2905,2907,2912,2918,3007,3009,3085,3096,3097,3098,3111,3116,3226,3233,3244,3247,3248,3250,3254,3260,3261,3263,3268,3273,3282,3287,3288,3290,3293,3295,3299,3430,3435,3437,3449,3451,3453,3454,3455,3457,3460,3461,3466,3469,3471,3473,3475,3477,3478,3479,3480,3486,3489,3490,3491,3493,3494,3495,3497,3498,3501,3504,3509,3518,3532,3535,3536,3539,3542,3571,3576,3671,3674,3676,3678,3679,3685,3689,3690,3692,3694,3698,3701,3702,3703,3706,3708,3710,3711,3712,3713,3719,3720,3723,3725,3726,3773,3776,3837,3841,3858,3862,3863,3884,4014,4017,4019,4020,4022,4025,4036,4038,4045,4047,4051,4053,4054,4056,4058,4059,4060,4065,4066,4068,4069,4073,4076,4078,4080,4083,4086,4088,4091,4095,4099,4100,4103,4105,4106,4110,4112,4114,4115,4118,4120,4121,4125,4126,4131,4135,4136,4284,4286,4288,4292,4293,4294,4297,4298,4299,4305,4449,4456,4459,4462,4472,4479,4482,4487,4488,4494,4497,4498,4519,4524,4530,4532,4534,4536,4550,4553,4556,4559,4563,4566,4668,4673,4675,4690,4694,4696,4704,4705,4707,4709,4710,4713,4715,4720,4725,4729,4747,4856,4858,4861,4876,4880,4884,4887,4889,4892,4894,4909,4998,5019,5020,5022,5024,5032,5034,5036,5037,5038,5040,5041,5046,5050,5053,5055,5059,5061,5064,5066,5071,5072,5075,5076,5077,5079,5091,5093,5096,5099,5103,5104,5110,5111,5113,5117,5118,5120,5124,5126,5128,5249,5253,5260,5264,5268,5270,5273,5276,5279,5280,5281,5283,5285,5286,5291,5293,5296,5299,5308,5441,5448,5449,5458,5466,5467,5469,5472,5473,5474,5479,5486,5488,5491,5508,5643,5645,5659,5664,5668,5672,5677,5683,5819,5821,5823,5825,5828,5830,5844,5850,5854,5855,5857,5859,5860,5865,5869,5871,5976,6001,6007,6009,6013,6015,6021,6024,6026,6028,6032,6038,6041,6049,6050,6053,6064,6071,6076,6086,6088,6089,6096,6099,6177,6185,6191,6194,6196,6197,6202,6209,6210,6215,6228,6244,6251,6371,6374,6376,6392,6398,6412,6563,6566,6571,6574,6578,6581,6589,6597,6610,6617,6623,6630,6727,6730,6735,6737,6739,6741,6757,6758,6759,6762,6765,6768,6771,6774,6777,6779,6782,6794,6801,6804,6818,6933,6936,6939,6941,6943,6944,6945,6948,6949,6954,6956,6958,6965,6967,6969,6978,6984,6985,6989,6992,7122,7126,7130,7132,7138,7144,7146,7147,7150,7154,7157,7164,7169,7285,7290,7292,7294,7296,7309,7318,7323,7326,7328,7329,7330,7331,7332,7336,7477,7484,7506,7512,7514,7516,7522,7523,7526,7527,7528,7532,7542,7545,7656,7665,7670,7672,7673,7674,7687,7688,7693,7694,7696,7697,7699,7700,7704,7706,7710,7721,7724,7733,7737,7738,7871,7873,7884,7887,7891,7895,7896,7897,7899,7901,7903,7907,7915,7918,7919,7922,7966,7971,7973,7977,7986,7988,7989,8006,8013,8026,8027,8032,8049,8057,8070,8076,8081,8083,8086,8100,8103,8105,8108,8110,8112,8118,8121,8123,8125,8190,8235,8237,8240,8243,8246,8251,8266,8276,8279,8301,8305,8307,8419,8423,8426,8428,8441,8448,8449,8453,8467,8474,8481,8596,8603,8606,8608,8613,8617,8623,8631,8641,8644,8647,8652,8771,8804,8809,8810,8813,8818,8820,8823,8831,8834,8837,8838,8883,8902,8930,8973,8976,8978,8990,8992,8995,8997,8998,9006,9011,9012,9014,9015,9016,9019,9022,9025,9027,9085,9125,9129,9145,9148,9150,9154,9156,9163,9170,9172,9174,9179,9180,9181,9183,9186,9187,9188,9190,9191,9193,9195,9196,9197,9198,9200,9202,9204,9207,9209,9211,9212,9215,9217,9219,9222,9223,9225,9240,9243,9247,9249,9253,9254,9257,9260,9261,9265,9267,9270,9277,9279,9285,9343,9350,9355,9358,9362,9364,9366,9368,9370,9372,9376,9378,9384,9386,9388,9390,9392,9394,9400,9402,9404,9406,9408,9410,9412,9414,9415,9416,9418,9420,9422,9423,9425,9426,9427,9430,9434,9439,9441,9443,9445,9450,9451,9467,9567,9574,9576,9588,9589,9591,9595,9598,9599,9601,9602,9603,9604,9605,9607,9610,9661,9682,9694,9725,9736,9738,9740,9743,9756,9760,9763,9777,9781,9787,9788,9790,9791,9883,9887,9892,9923,9926,9929,9933,9935,9939,9944,9946,9947,9950,9954,9956,9957,9959,9963,9967,9970,9973,9974,9978,10002,10004,10006,10032,10035,10134,10142,10143,10145,10147,10148,10152,10157,10162,10163,10166,10167,10170,10172,10174,10177,10179,10181,10182,10184,10187,10190,10322,10333,10336,10344,10352,10357,10361,10362,10366,10373,10374,10378,10380,10381,10383,10385,10389,10394,10399,10401,10402,10407,10410,10476,10503,10504,10506,10509,10510,10512,10514,10516,10519,10520,10521,10525,10527,10530,10533,10534,10536,10546,10551,10573,10578,10697,10699,10700,10702,10703,10705,10708,10715,10718,10724,10726,10742,10744,10746,10748,10750,10752,10754,10756,10758,10760,10762,10764,10766,10768,10770,10772,10774,10776,10778,10780,10782,10784,10786,10788,10790,10794,10796,10798,10800,10802,10808,10822,10824,10826,10828,10831,10835,10836,10838,10843,10846,10850,10854,10856,10857,10864,10869,10871,10876,10881,10892,10894,10896,10898,10904,10967,10992,10994,10996,10999,11003,11004,11006,11007,11009,11014,11016,11017,11022,11024,11025,11027,11028,11032,11036,11037,11039,11041,11157,11159,11172,11175,11177,11178,11179,11181,11184,11185,11191,11193,11194,11197,11199,11202,11207,11208,11215,11219,11222,11227,11235,11342,11354,11356,11360,11361,11364,11369,11375,11376,11379,11380,11387,11389,11411,11481,11508,11509,11511,11514,11515,11518,11521,11525,11528,11530,11533,11540,11542,11545,11547,11548,11550,11552,11554,11558,11573,11577,11594,11661,11690,11692,11694,11697,11710,11712,11714,11715,11718,11721,11723,11726,11728,11732,11733,11734,11736,11738,11739,11743,11745,11747,11750,11751,11754,11756,11760,11763,11767,11771,11774,11775,11776,11777,11779,11782,11786,11790,11792,11795,11797,11799,11800,11803,11809,11811,11816,11820,11823,11827,11832,11935,11938,11942,11944,11945,11947,11950,11952,11955,11963,11967,11971,11974,11975,11978,12008,12091,12093,12094,12097,12100,12102,12104,12105,12106,12107,12110,12111,12117,12119,12121,12124,12126,12128,12129,12130,12131,12134,12138,12141,12143,12148,12150,12153,12157,12158,12159,12161,12165,12167,12285,12290,12292,12298,12300,12307,12308,12310,12312,12314,12315,12317,12319,12333,12336,12340,12341,12342,12344,12346,12347,12349,12351,12353,12356,12386,12389,12483,12489,12498,12500,12501,12503,12505,12507,12508,12509,12521,12522,12525,12526,12538,12541,12548,12550,12552,12560,12562,12564,12565,12567,12569,12572,12574,12575,12578,12579,12582,12583,12585,12592,12598,12601,12603,12606,12608,12612,12615,12617,12619,12623,12663,12667,12708,12713,12717,12721,12727,12741,12742,12745,12746,12750,12753,12756,12762,12765,12767,12768,12771,12853,12855,12869,12872,12875,12878,12880,12883,12884,12886,12887,12890,12891,12893,12897,12900,12902,12905,12908,12911,12912,12915,12919,12922,12925,12929,12931,12933,12936,13016,13023,13042,13047,13049,13053,13056,13062,13065,13069,13072,13073,13077,13078,13081,13084,13090,13094,13097,13101,13105,13123,13135,13216,13220,13229,13231,13237,13240,13256,13260,13269,13387,13401,13404,13405,13406,13410,13411,13415,13417,13420,13421,13433,13435,13447,13450,13452,13454,13458,13460,13532,13536,13537,13545,13552,13558,13559,13562,13564,13565,13569,13572,13580,13582,13583,13587,13588,13591,13594,13601,13616,13620,13621,13624,13627,13632,13739,13744,13751,13757,13764,13769,13774,13775,13778,13780,13784,13785,13789,13793,13795,13800,13925, ptg000001l:57999-71965:m54329U_210323_190418/111871348/ccs block 128,0,128,0 Ignored m6A +ptg000001l 58069 71634 5mC 0 + 58069 71634 139,69,19,1 45 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,37,48,871,928,1019,2617,6034,6036,6043,6585,6899,6904,6933,6963,7589,8012,9441,9489,9833,9856,9863,9971,10012,10016,10049,10973,11391,11424,11875,12383,12396,12399,12605,13028,13070,13145,13205,13207,13215,13519,13530,13551,13562,13564, ptg000001l:57999-71965:m54329U_210323_190418/111871348/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 58200 71761 NUC 0 + 58200 71761 169,169,169,1 65 156,78,129,94,104,119,121,117,159,144,160,97,104,125,123,88,75,109,130,94,110,129,147,143,101,108,88,120,132,134,135,104,77,119,150,96,114,129,115,140,110,132,109,111,114,151,99,99,90,98,131,118,115,106,118,112,102,112,117,93,81,79,80,117,106, 0,197,317,626,802,971,1116,1305,1444,1619,1887,2048,2193,2363,2596,2741,2832,2939,3122,3399,3549,3707,3959,4128,4389,4570,4732,4951,5131,5331,5506,5694,5922,6074,6235,6453,6641,6815,6992,7159,7368,7561,7948,8130,8304,8475,8848,9290,9615,9858,10013,10401,10864,11058,11212,11400,11655,11801,11990,12212,12594,12759,12958,13092,13455, ptg000001l:57999-71965:m54329U_210323_190418/111871348/ccs block 169,169,169,0 Ignored NUC +ptg000001l 58646 69064 FIRE 0 + 58646 69064 225,0,0,1 2 180,345, 0,10073, ptg000001l:57999-71965:m54329U_210323_190418/111871348/ccs block 225,0,0,0 Ignored FIRE +ptg000001l 58172 71823 LINKER 0 + 58172 71823 147,112,219,1 64 28,41,42,82,65,26,68,22,16,124,1,48,66,108,22,3,32,74,147,56,48,123,22,118,80,54,131,60,68,41,53,124,75,42,68,92,60,48,52,69,83,255,73,63,57,222,343,226,153,57,257,79,48,70,143,44,77,105,289,84,120,54,246,62, 0,184,303,748,934,1118,1265,1450,1631,1791,2075,2173,2325,2516,2747,2857,2935,3076,3280,3521,3687,3864,4134,4299,4518,4706,4848,5099,5291,5493,5669,5826,6027,6221,6413,6577,6783,6972,7135,7327,7506,7721,8085,8269,8446,8654,8975,9417,9733,9984,10172,11007,11192,11358,11540,11785,11941,12135,12333,12703,12866,13066,13237,13589, ptg000001l:57999-71965:m54329U_210323_190418/111871348/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 58030 74216 m6A 0 + 58030 74216 128,0,128,1 2169 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,5,113,116,118,121,124,146,148,151,153,155,195,208,506,509,512,514,517,518,521,522,528,535,538,539,541,542,545,578,580,748,773,795,915,917,920,923,933,934,937,939,942,946,971,1107,1109,1112,1116,1122,1124,1126,1129,1130,1132,1134,1136,1138,1140,1162,1164,1166,1168,1170,1172,1174,1176,1178,1182,1184,1185,1186,1188,1190,1192,1341,1346,1350,1355,1356,1358,1670,1876,1877,1879,1881,1890,2037,2041,2046,2051,2055,2056,2057,2059,2061,2064,2066,2075,2078,2086,2088,2231,2236,2238,2242,2244,2245,2250,2251,2254,2256,2258,2406,2413,2415,2416,2418,2420,2422,2424,2426,2428,2429,2432,2434,2435,2439,2443,2447,2450,2455,2461,2463,2464,2466,2467,2468,2473,2474,2478,2480,2482,2483,2484,2486,2488,2490,2491,2492,2495,2497,2509,2511,2513,2514,2518,2520,2524,2790,2802,2804,2807,2812,2814,2839,2841,2842,2844,2846,2852,2854,2856,2858,3133,3184,3392,3408,3432,3437,3447,3449,3458,3465,3469,3619,3622,3625,3654,3813,3816,3829,3833,4002,4007,4009,4011,4012,4014,4017,4022,4027,4034,4036,4037,4039,4043,4045,4046,4048,4050,4051,4060,4065,4068,4070,4339,4341,4348,4357,4359,4360,4365,4368,4369,4371,4373,4375,4376,4377,4380,4382,4384,4388,4390,4394,4396,4397,4399,4400,4403,4405,4410,4417,4419,4422,4431,4439,4542,4544,4545,4548,4551,4553,4555,4556,4558,4560,4561,4562,4563,4567,4568,4569,4572,4581,4583,4585,4586,4587,4591,4593,4597,4598,4599,4602,4603,4604,4606,4609,4620,4621,4626,4627,4632,4633,4636,4639,4642,4644,4660,4735,4737,4739,4756,4758,4760,4762,4764,4767,4770,4771,4772,4776,4779,4781,4782,4783,4785,4787,4789,4790,4793,4796,4797,4798,4803,4808,4810,4812,4814,4816,4821,4828,4832,4839,4841,4848,4850,4852,4853,4856,4858,4860,4862,4863,4864,4868,4872,4876,4879,4881,4884,4886,4892,4894,4896,4897,4901,4903,4905,4906,4908,4912,4914,4915,4918,4922,4926,4929,4933,4941,4950,5024,5026,5028,5029,5030,5032,5033,5047,5050,5051,5053,5056,5058,5061,5063,5064,5067,5068,5069,5071,5076,5081,5083,5091,5093,5095,5100,5105,5109,5110,5112,5116,5118,5206,5213,5216,5219,5220,5221,5222,5232,5233,5235,5237,5241,5242,5245,5246,5252,5256,5259,5260,5265,5268,5271,5272,5275,5277,5283,5285,5288,5291,5295,5298,5312,5401,5411,5412,5416,5417,5419,5424,5425,5427,5430,5433,5434,5435,5440,5441,5443,5445,5447,5449,5450,5452,5453,5455,5456,5458,5459,5461,5464,5465,5466,5471,5473,5478,5480,5483,5485,5488,5491,5494,5496,5497,5500,5502,5511,5697,5705,5710,5820,5887,5889,5892,5894,5910,5919,6056,6065,6068,6070,6078,6080,6081,6084,6188,6194,6201,6202,6207,6210,6211,6212,6213,6215,6217,6219,6220,6221,6224,6225,6226,6228,6232,6234,6236,6238,6240,6241,6243,6245,6246,6248,6249,6251,6253,6255,6256,6257,6260,6262,6264,6267,6270,6279,6282,6283,6285,6286,6288,6289,6290,6292,6293,6295,6296,6297,6299,6304,6313,6317,6318,6322,6434,6451,6453,6455,6459,6471,6473,6475,6476,6478,6479,6480,6482,6484,6485,6493,6495,6496,6500,6505,6642,6644,6649,6650,6652,6656,6660,6661,6663,6664,6665,6669,6671,6673,6674,6684,6689,6690,6691,6693,6705,6803,6806,6810,6812,6816,6818,6820,6822,6823,6825,6826,6827,6829,6837,6839,6843,6846,6851,6856,6858,6861,6862,6868,6869,6871,6874,6875,6878,6880,6881,6882,6884,6886,6887,6891,6898,6903,6970,6990,6993,6994,6997,7005,7009,7010,7012,7014,7021,7024,7026,7027,7029,7031,7033,7034,7037,7039,7042,7043,7047,7049,7051,7053,7055,7057,7058,7061,7062,7065,7068,7073,7075,7076,7079,7080,7081,7083,7085,7086,7091,7093,7106,7127,7227,7234,7237,7242,7243,7247,7249,7250,7254,7259,7261,7263,7265,7267,7270,7272,7274,7277,7279,7282,7284,7286,7288,7289,7291,7294,7297,7310,7315,7318,7321,7322,7323,7324,7328,7329,7434,7440,7442,7444,7445,7446,7449,7454,7455,7457,7459,7462,7463,7465,7469,7471,7473,7476,7478,7479,7482,7483,7486,7584,7586,7600,7605,7607,7608,7610,7614,7619,7621,7626,7631,7635,7637,7639,7640,7644,7646,7648,7650,7651,7656,7658,7664,7668,7675,7676,7689,7691,7692,7693,7696,7812,7817,7821,7823,7828,7831,7832,7835,7836,7839,7840,7843,7847,7851,7859,7861,7863,7865,7871,7875,7876,7879,7883,7887,7888,7891,7893,7905,7914,8030,8032,8037,8041,8043,8049,8054,8060,8061,8062,8064,8068,8069,8072,8075,8076,8078,8079,8081,8085,8087,8090,8092,8095,8097,8100,8104,8117,8186,8205,8209,8210,8212,8218,8221,8224,8227,8229,8232,8235,8237,8242,8246,8258,8262,8265,8268,8376,8393,8400,8402,8403,8407,8411,8413,8415,8418,8420,8422,8433,8441,8558,8572,8576,8578,8580,8582,8584,8586,8588,8590,8595,8598,8600,8601,8603,8606,8607,8609,8612,8613,8615,8617,8622,8623,8626,8627,8629,8631,8633,8636,8637,8639,8641,8644,8646,8648,8650,8651,8652,8655,8657,8658,8660,8662,8664,8666,8668,8671,8801,8894,8899,8901,8904,8908,8914,8922,8924,8929,8955,8958,8959,8970,8982,8984,8987,8989,8990,9099,9103,9105,9106,9111,9112,9114,9117,9121,9122,9127,9130,9134,9137,9140,9142,9146,9151,9154,9158,9252,9255,9269,9271,9274,9277,9287,9291,9294,9295,9296,9299,9301,9302,9304,9305,9307,9309,9310,9313,9319,9321,9322,9325,9329,9332,9333,9335,9337,9339,9340,9342,9343,9345,9347,9349,9351,9353,9354,9356,9358,9360,9362,9364,9366,9368,9370,9372,9374,9376,9378,9496,9503,9506,9509,9511,9522,9524,9527,9530,9532,9535,9536,9538,9539,9541,9543,9544,9548,9550,9551,9552,9555,9562,9566,9568,9574,9581,9587,9590,9680,9686,9693,9697,9700,9702,9704,9707,9708,9711,9713,9723,9725,9728,9730,9732,9735,9737,9744,9748,9749,9752,9754,9758,9760,9835,9839,9843,9845,9853,9857,9860,9862,9865,9867,9874,9875,9879,9884,9889,9890,9893,9909,9914,9916,9918,9921,9925,9927,9931,9936,9938,9939,9946,9949,9951,9955,9959,9962,9966,10040,10046,10048,10059,10060,10065,10069,10071,10073,10076,10081,10083,10090,10092,10096,10098,10101,10104,10110,10112,10113,10114,10115,10119,10126,10130,10134,10135,10137,10140,10151,10269,10272,10274,10275,10276,10278,10282,10285,10289,10291,10293,10301,10303,10304,10306,10307,10314,10316,10318,10319,10330,10331,10334,10336,10340,10349,10352,10353,10354,10358,10360,10484,10486,10488,10492,10493,10495,10496,10498,10500,10501,10502,10504,10506,10508,10510,10511,10512,10513,10517,10519,10520,10522,10525,10526,10528,10529,10532,10535,10536,10538,10540,10541,10543,10689,10691,10692,10694,10695,10697,10698,10700,10701,10703,10705,10707,10710,10715,10718,10720,10723,10724,10728,10730,10732,10734,10736,10738,10740,10742,10744,10746,10748,10750,10752,10754,10756,10758,10760,10846,10852,10855,10866,10872,10886,10888,10892,10893,10897,10899,10900,10902,10907,10908,10910,10912,10915,10918,10921,10928,10932,10934,10946,10951,10955,11053,11069,11071,11075,11078,11080,11082,11083,11086,11092,11093,11098,11102,11105,11107,11109,11110,11111,11114,11116,11117,11120,11121,11125,11127,11128,11130,11131,11132,11137,11138,11140,11142,11144,11145,11147,11149,11152,11153,11156,11159,11273,11286,11289,11292,11294,11303,11307,11308,11310,11321,11326,11329,11331,11332,11334,11344,11346,11348,11350,11353,11354,11356,11359,11361,11362,11364,11367,11368,11371,11461,11483,11488,11489,11491,11493,11495,11498,11500,11501,11503,11506,11510,11516,11517,11520,11521,11522,11525,11530,11532,11534,11537,11542,11550,11556,11616,11674,11676,11678,11682,11684,11686,11689,11690,11696,11698,11700,11702,11704,11706,11707,11710,11713,11715,11718,11720,11725,11728,11730,11837,11845,11851,11856,11857,11858,11859,11863,11864,11866,11867,11869,11872,11876,11879,11881,11882,11887,11890,11895,11898,11900,11902,11903,11905,11909,11913,11918,11919,11923,11925,11927,11930,11934,11936,11937,11939,11942,11944,11947,11953,12027,12030,12040,12042,12045,12047,12052,12055,12057,12061,12063,12070,12071,12074,12075,12076,12078,12080,12083,12085,12086,12089,12090,12091,12092,12094,12099,12109,12111,12113,12116,12118,12120,12122,12136,12140,12141,12142,12241,12243,12247,12249,12254,12255,12261,12263,12267,12270,12271,12272,12275,12276,12277,12281,12282,12284,12287,12290,12291,12292,12296,12297,12299,12300,12302,12304,12306,12307,12309,12311,12312,12313,12314,12317,12322,12324,12325,12328,12331,12333,12334,12336,12339,12341,12343,12345,12348,12433,12440,12446,12448,12451,12452,12455,12456,12459,12461,12465,12469,12470,12472,12473,12475,12476,12477,12478,12481,12483,12485,12487,12490,12492,12493,12495,12497,12499,12500,12501,12506,12508,12510,12512,12517,12518,12520,12524,12531,12533,12538,12540,12542,12544,12548,12552,12617,12636,12640,12643,12648,12653,12655,12657,12661,12668,12671,12675,12677,12679,12680,12683,12685,12686,12688,12689,12691,12694,12695,12698,12700,12702,12703,12705,12706,12709,12713,12719,12722,12724,12725,12730,12733,12734,12737,12738,12739,12742,12745,12748,12830,12831,12861,12864,12867,12869,12872,12875,12876,12878,12879,12882,12885,12887,12889,12892,12894,12897,12900,12903,12904,12907,12908,12911,12914,12917,12918,12921,12923,12925,12928,12929,12931,12932,12933,12934,12938,12940,12941,12943,12953,12957,12982,12986,12992,12994,13012,13091,13093,13094,13097,13101,13102,13106,13112,13115,13119,13122,13127,13131,13133,13140,13149,13150,13152,13154,13156,13158,13168,13170,13176,13196,13248,13252,13270,13274,13282,13283,13288,13296,13298,13301,13303,13304,13306,13311,13313,13315,13318,13320,13321,13322,13326,13327,13333,13335,13337,13340,13343,13345,13346,13347,13350,13353,13355,13356,13358,13360,13364,13365,13372,13379,13452,13462,13464,13466,13476,13484,13486,13488,13490,13492,13493,13495,13499,13502,13504,13506,13508,13510,13513,13516,13517,13518,13519,13524,13526,13529,13531,13534,13537,13541,13544,13546,13548,13549,13550,13551,13554,13556,13561,13564,13565,13566,13572,13574,13575,13576,13579,13580,13583,13586,13593,13702,13705,13712,13716,13718,13721,13734,13737,13743,13749,13872,13874,13876,13878,13879,13885,13887,13894,13896,13897,13914,14031,14037,14049,14052,14055,14057,14058,14062,14065,14068,14069,14072,14073,14075,14080,14083,14088,14093,14106,14112,14126,14227,14229,14231,14233,14236,14239,14242,14243,14245,14246,14249,14251,14254,14255,14257,14258,14259,14261,14264,14265,14266,14268,14269,14278,14281,14283,14287,14291,14292,14294,14298,14304,14382,14387,14388,14394,14396,14397,14398,14402,14403,14408,14414,14415,14418,14425,14429,14431,14432,14433,14436,14441,14442,14444,14445,14447,14448,14451,14453,14457,14462,14464,14466,14477,14482,14484,14487,14491,14492,14493,14495,14496,14498,14501,14515,14557,14559,14561,14570,14572,14575,14578,14580,14581,14584,14589,14596,14598,14603,14604,14610,14616,14619,14620,14621,14625,14627,14630,14631,14632,14633,14636,14637,14638,14640,14642,14655,14657,14660,14661,14764,14770,14774,14775,14777,14778,14780,14782,14784,14785,14788,14791,14792,14802,14804,14808,14809,14812,14814,14815,14816,14818,14819,14821,14824,14826,14827,14830,14832,14833,14835,14837,14840,14842,14843,14849,14851,14852,14855,14856,14863,14865,14867,14868,14869,14871,14872,14873,14877,14981,14983,14984,14986,15005,15007,15008,15009,15011,15012,15014,15016,15017,15018,15027,15031,15032,15033,15035,15037,15038,15072,15141,15147,15150,15152,15154,15156,15158,15162,15167,15170,15171,15174,15175,15176,15180,15184,15187,15189,15199,15205,15208,15209,15211,15214,15215,15217,15218,15219,15221,15225,15227,15229,15231,15236,15239,15241,15244,15248,15252,15254,15257,15260,15262,15264,15267,15275,15282,15318,15336,15342,15343,15345,15347,15352,15355,15356,15363,15364,15366,15369,15372,15373,15374,15377,15379,15381,15383,15385,15388,15389,15392,15395,15398,15401,15402,15406,15408,15410,15411,15413,15414,15416,15417,15418,15420,15425,15426,15427,15429,15430,15433,15436,15437,15440,15441,15443,15447,15448,15452,15461,15463,15465,15468,15469,15474,15479,15501,15589,15590,15592,15593,15595,15597,15603,15608,15609,15612,15613,15615,15617,15622,15627,15628,15631,15633,15634,15641,15644,15647,15657,15659,15661,15746,15766,15769,15771,15772,15774,15775,15777,15780,15781,15785,15788,15791,15792,15793,15796,15798,15799,15800,15804,15805,15808,15812,15815,15817,15820,15821,15823,15825,15826,15828,15829,15831,15834,15845,15846,15857,15969,15971,15974,15976,15979,15981,15986,15987,15988,15990,15992,15994,15997,15998,16000,16003,16005,16008,16009,16011,16012,16013,16015,16016,16018,16019,16020,16021,16026,16031,16036,16042,16045,16049,16051,16052,16174,16182,16185, ptg000001l:58014-74219:m54329U_210323_190418/14615259/ccs block 128,0,128,0 Ignored m6A +ptg000001l 58106 73763 5mC 0 + 58106 73763 139,69,19,1 64 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,11,801,982,1947,1959,2128,2510,2512,2611,2651,3423,4729,5398,5831,5862,5945,5952,5997,5999,6006,6426,6555,6641,6757,6818,6862,6867,6896,6926,7552,7975,9796,9819,9826,9934,9975,11012,11014,11046,11354,11741,12496,12597,13168,13170,13178,13420,13482,13493,13514,13525,13527,13563,13942,14196,14515,15052,15066,15101,15118,15212,15285,15656, ptg000001l:58014-74219:m54329U_210323_190418/14615259/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 58239 73999 NUC 0 + 58239 73999 169,169,169,1 65 297,167,119,135,148,311,205,146,142,147,265,273,207,149,158,168,268,102,87,88,185,176,136,103,111,136,97,99,104,97,115,115,100,107,116,129,92,108,93,117,89,117,123,145,85,97,113,89,117,106,98,84,81,78,108,122,116,100,77,102,103,102,87,84,111, 0,372,587,763,984,1150,1462,1682,1880,2050,2316,2650,2976,3261,3446,3625,3862,4231,4910,5104,5303,5502,5711,5876,6114,6297,6497,6919,7121,7278,7488,7706,7896,8060,8233,8463,8593,8782,8950,9170,9382,9943,10152,10335,10552,10747,10951,11163,11348,11522,11934,12140,12540,12804,13385,13541,13706,13918,14096,14453,14669,14830,15293,15453,15649, ptg000001l:58014-74219:m54329U_210323_190418/14615259/ccs block 169,169,169,0 Ignored NUC +ptg000001l 73171 73532 FIRE 0 + 73171 73532 255,0,0,1 1 361, 0, ptg000001l:58014-74219:m54329U_210323_190418/14615259/ccs block 255,0,0,0 Ignored FIRE +ptg000001l 58143 74083 LINKER 0 + 58143 74083 147,112,219,1 65 96,75,48,57,86,18,1,15,52,28,119,69,53,78,36,21,69,101,577,107,111,14,33,29,135,72,64,325,103,53,113,103,75,64,66,114,1,97,60,127,95,472,92,60,72,110,107,99,96,57,306,108,316,183,503,48,43,96,78,280,114,58,73,112,84, 0,393,635,802,994,1228,1557,1763,1924,2118,2293,2677,3019,3279,3506,3700,3889,4226,4429,5093,5288,5584,5774,5943,6075,6321,6529,6690,7114,7321,7471,7699,7917,8092,8263,8445,8688,8781,8986,9139,9383,9567,10156,10371,10576,10733,10940,11160,11348,11561,11724,12128,12320,12717,12978,13589,13759,13918,14114,14269,14651,14868,15476,15633,15856, ptg000001l:58014-74219:m54329U_210323_190418/14615259/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 64071 78138 m6A 0 - 64071 78138 128,0,128,1 1168 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,4,11,13,15,22,24,27,29,37,39,50,52,191,193,195,197,199,202,204,208,210,212,214,215,216,219,221,223,229,234,370,376,379,381,383,385,386,389,391,393,396,397,400,414,416,420,421,425,430,432,434,435,437,438,441,443,444,445,450,454,455,459,463,464,595,598,601,603,608,609,611,622,643,646,648,649,650,652,654,658,661,664,665,668,672,673,678,690,692,802,804,820,830,833,834,837,839,840,843,845,911,1002,1006,1010,1012,1014,1016,1017,1020,1021,1024,1027,1032,1034,1035,1038,1039,1040,1044,1186,1192,1206,1209,1217,1222,1224,1226,1229,1231,1236,1238,1239,1241,1245,1250,1252,1253,1256,1412,1418,1421,1422,1424,1428,1430,1432,1435,1438,1445,1609,1621,1644,1647,1648,1650,1663,1802,1806,1810,1814,1818,1820,1822,1824,1826,1834,1835,1842,1847,1850,1852,1854,2000,2008,2021,2023,2027,2038,2145,2147,2151,2153,2159,2164,2171,2176,2180,2183,2186,2188,2190,2194,2197,2217,2221,2227,2230,2370,2399,2405,2418,2422,2425,2427,2432,2440,2445,2581,2582,2588,2592,2595,2596,2598,2600,2603,2610,2611,2614,2616,2617,2619,2622,2623,2625,2630,2639,2760,2772,2774,2777,2778,2781,2782,2784,2785,2795,2800,2802,2803,2805,2806,2808,2810,2813,2818,2821,2822,2825,2827,2830,2834,2836,2839,2842,2843,2845,2846,2848,2849,2853,2855,2858,2860,2863,2867,2871,2879,2889,3001,3002,3004,3006,3008,3019,3022,3024,3026,3029,3036,3037,3038,3039,3043,3055,3058,3065,3070,3071,3076,3182,3185,3189,3198,3200,3204,3205,3208,3211,3212,3214,3216,3228,3230,3233,3236,3253,3255,3266,3269,3273,3381,3387,3390,3394,3396,3402,3404,3405,3409,3410,3412,3414,3418,3419,3421,3425,3430,3433,3436,3438,3589,3593,3594,3598,3600,3605,3612,3615,3619,3621,3626,3628,3630,3631,3639,3667,3791,3794,3796,3798,3802,3804,3806,3808,3812,3816,3819,3821,3824,3826,3830,3833,3834,3836,3838,3841,3843,3845,3849,3852,3858,3865,3870,3874,3875,3877,3880,3884,3982,3999,4004,4007,4008,4028,4032,4035,4040,4042,4044,4049,4051,4055,4057,4063,4079,4081,4083,4198,4202,4226,4228,4231,4233,4234,4235,4237,4248,4250,4263,4267,4399,4403,4411,4414,4416,4427,4433,4437,4442,4443,4447,4451,4452,4454,4455,4457,4459,4460,4461,4463,4465,4467,4470,4471,4475,4476,4478,4479,4481,4616,4647,4648,4650,4651,4653,4656,4662,4666,4669,4674,4825,4831,4838,4844,4845,4847,4859,4861,4869,4874,4883,4884,4891,4992,5007,5012,5024,5028,5030,5035,5037,5039,5041,5042,5045,5051,5052,5059,5061,5064,5066,5069,5223,5229,5232,5244,5245,5248,5251,5262,5266,5442,5447,5448,5450,5459,5465,5469,5472,5475,5476,5479,5484,5491,5633,5635,5637,5641,5643,5645,5648,5655,5659,5661,5663,5665,5666,5669,5672,5674,5677,5679,5683,5689,5694,5696,5705,5711,5714,5828,5831,5835,5838,5840,5841,5846,5849,5854,5861,5864,5868,5889,6020,6022,6028,6030,6033,6034,6035,6037,6039,6040,6042,6045,6200,6202,6204,6206,6211,6213,6214,6216,6217,6220,6230,6231,6234,6235,6236,6241,6243,6246,6249,6250,6251,6255,6261,6410,6414,6415,6418,6420,6424,6428,6434,6440,6442,6444,6446,6454,6459,6574,6587,6591,6593,6595,6602,6607,6614,6616,6620,6625,6626,6627,6630,6634,6636,6638,6639,6642,6644,6647,6650,6654,6657,6659,6661,6662,6664,6665,6672,6678,6681,6684,6794,6804,6806,6812,6820,6823,6826,6828,6829,6831,6834,6835,6837,6841,6844,6846,6851,6853,6854,6856,6859,6862,6866,6870,6873,7010,7016,7020,7021,7024,7028,7030,7032,7035,7039,7041,7045,7050,7053,7056,7060,7065,7071,7188,7191,7207,7220,7223,7226,7227,7366,7368,7371,7378,7382,7384,7386,7401,7403,7405,7407,7411,7416,7552,7567,7571,7572,7575,7578,7583,7594,7597,7601,7606,7609,7616,7776,7779,7783,7786,7790,7793,7801,7804,7808,7813,7817,7927,7931,7932,7937,7939,7947,7950,7959,7963,7966,7969,7971,7972,7976,7979,7980,7981,7983,7984,7985,7987,7991,7993,7997,8002,8011,8012,8014,8016,8017,8024,8159,8165,8167,8181,8201,8220,8313,8323,8353,8355,8357,8361,8368,8374,8377,8384,8388,8392,8395,8516,8525,8529,8531,8534,8537,8539,8540,8543,8553,8557,8562,8563,8567,8575,8578,8579,8580,8586,8589,8601,8736,8737,8739,8741,8743,8757,8761,8763,8767,8768,8771,8773,8775,8778,8780,8964,8966,8968,8970,8973,8978,8990,9126,9129,9133,9139,9141,9143,9148,9149,9158,9164,9167,9168,9170,9178,9180,9184,9186,9188,9190,9195,9198,9203,9207,9213,9284,9314,9340,9342,9344,9347,9348,9351,9354,9361,9365,9389,9487,9492,9497,9525,9528,9530,9532,9536,9554,9556,9699,9705,9713,9715,9721,9728,9736,9739,9873,9875,9883,9885,9888,9889,9891,9897,9899,9903,9906,9909,9912,9919,9935,10024,10045,10051,10060,10073,10076,10088,10205,10210,10213,10215,10220,10224,10227,10235,10237,10239,10241,10243,10245,10249,10251,10252,10413,10415,10431,10437,10443,10453,10459,10462,10464,10572,10579,10581,10584,10591,10593,10595,10608,10610,10613,10618,10620,10621,10623,10626,10628,10629,10630,10633,10635,10638,10641,10642,10644,10647,10649,10650,10652,10655,10658,10676,10784,10786,10793,10797,10799,10812,10815,10818,10819,10821,10822,10824,10827,10828,10829,10833,10836,10838,10852,10853,10859,10866,10868,10871,10873,11000,11004,11011,11013,11019,11029,11032,11157,11158,11161,11169,11171,11172,11176,11177,11179,11182,11196,11197,11199,11202,11205,11207,11209,11214,11221,11395,11398,11399,11400,11407,11548,11549,11550,11553,11554,11555,11558,11560,11570,11573,11576,11579,11581,11583,11585,11587,11590,11591,11599,11602,11607,11611,11614,11622,11751,11765,11767,11769,11772,11783,11784,11787,11788,11790,11792,11793,11794,11796,11799,11802,11805,11807,11812,11819,11820,11827,11829,11833,11837,11839,11841,11959,11974,11977,11982,11984,11987,11997,11999,12001,12004,12005,12008,12020,12024,12027,12028,12029,12125,12148,12152,12154,12158,12160,12162,12165,12167,12188,12204,12206,12335,12339,12341,12343,12347,12349,12351,12353,12354,12356,12358,12364,12367,12373,12376,12378,12511,12515,12517,12519,12534,12536,12537,12543,12546,12549,12550,12551,12553,12555,12556,12558,12559,12563,12569,12573,12577,12578,12579,12582,12583,12585,12586,12587,12589,12590,12592,12595,12597,12598,12601,12603,12606,12607,12609,12734,12739,12745,12750,12751,12754,12770,12776,12786,12843,12933,12935,12937,12942,12945,12946,12952,12953,12955,12969,12977,13132,13151,13324,13333,13557,13681,13863,13870,13872,13878,14024,14026,14031,14037,14049,14056,14065,14066, ptg000001l:63954-78146:m54329U_210323_190418/19792629/ccs block 128,0,128,0 Ignored m6A +ptg000001l 64052 78054 5mC 0 - 64052 78054 139,69,19,1 58 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,18,52,54,61,481,603,812,828,833,917,922,951,1607,2030,2549,3507,3851,3874,3881,3978,4030,4034,4087,5017,5067,5069,5101,5306,5391,5505,5796,6580,7046,7088,7223,7225,7233,7537,7569,7580,7997,8570,9107,9121,9156,9267,9340,10197,10227,10251,10267,11531,12482,13306,13611,13823,14001, ptg000001l:63954-78146:m54329U_210323_190418/19792629/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 64124 78095 NUC 0 - 64124 78095 169,169,169,1 72 138,135,130,109,156,141,155,163,138,145,106,139,135,120,111,105,107,150,151,97,114,131,134,150,100,153,175,141,113,130,154,148,114,109,136,116,138,135,159,109,134,92,120,134,183,135,100,97,142,133,88,116,160,107,107,126,124,173,140,128,117,95,128,132,124,146,154,172,223,123,181,145, 0,182,412,640,793,992,1204,1393,1611,1802,1986,2178,2393,2587,2837,3024,3221,3386,3587,3832,4031,4215,4429,4622,4839,5017,5214,5439,5662,5837,5993,6209,6407,6632,6821,7019,7175,7364,7564,7765,7972,8168,8343,8549,8728,8938,9161,9337,9504,9687,9883,10036,10200,10412,10624,10821,10980,11169,11355,11570,11789,11977,12154,12326,12557,12734,12925,13099,13281,13505,13629,13826, ptg000001l:63954-78146:m54329U_210323_190418/19792629/ccs block 169,169,169,0 Ignored NUC +ptg000001l 64071 77950 LINKER 0 - 64071 77950 147,112,219,1 72 53,44,95,98,44,43,71,34,55,53,39,86,76,59,130,76,92,58,51,94,102,70,83,59,67,78,44,50,82,62,26,62,50,111,80,62,40,51,65,42,98,62,83,86,45,27,88,76,70,41,63,65,48,52,105,90,33,65,13,75,91,71,82,44,99,53,45,20,10,1,1,16, 0,191,370,595,802,1002,1186,1412,1609,1802,2000,2145,2370,2581,2760,3001,3182,3381,3589,3791,3982,4198,4399,4616,4825,4992,5223,5442,5633,5828,6020,6200,6410,6574,6794,7010,7188,7366,7552,7776,7927,8159,8313,8516,8736,8964,9126,9314,9487,9699,9873,10024,10205,10413,10572,10784,11000,11157,11395,11548,11751,11959,12125,12335,12511,12734,12933,13132,13324,13557,13681,13863, ptg000001l:63954-78146:m54329U_210323_190418/19792629/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 64075 91942 m6A 0 - 64075 91942 128,0,128,1 803 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,20,23,25,33,35,180,187,191,195,196,198,200,201,203,204,217,222,226,230,372,389,396,401,406,408,410,412,434,437,439,455,594,597,642,645,646,816,817,829,833,998,1002,1006,1010,1017,1023,1028,1220,1222,1225,1227,1417,1424,1426,1428,1592,1599,1767,1778,1779,1783,1786,1949,1960,1963,2100,2113,2116,2119,2121,2152,2153,2155,2216,2510,2513,2519,2531,2533,2535,2543,2751,2756,2757,2760,2966,2968,2969,2972,3149,3151,3154,3156,3170,3172,3176,3187,3190,3194,3196,3204,3345,3347,3351,3355,3390,3392,3398,3545,3748,3910,4076,4079,4089,4289,4291,4447,4450,4461,4474,4480,4643,4644,4647,4652,4662,4665,4673,4675,4687,4689,4691,4693,4695,4815,4843,4855,4857,4870,4873,4876,4880,4883,4887,4889,4890,4892,4895,4900,4901,4906,4910,4912,4916,5064,5262,5265,5446,5448,5455,5461,5465,5471,5472,5480,5631,5633,5637,5639,5644,5662,5665,5670,5818,5824,5834,5997,6016,6018,6196,6198,6216,6222,6236,6388,6399,6401,6403,6406,6407,6411,6559,6564,6570,6583,6587,6591,6595,6603,6608,6610,6612,6614,6615,6616,6626,6630,6632,6786,6788,6790,6792,6793,6795,6796,6800,6802,6805,6806,6808,6810,6816,6818,6819,6821,6822,6824,6825,6827,6830,6833,6840,6849,7009,7012,7016,7017,7020,7184,7187,7207,7380,7382,7397,7399,7403,7407,7409,7412,7548,7563,7571,7574,7579,7581,7587,7593,7597,7600,7602,7603,7605,7779,7972,7976,7983,7987,7989,7993,7998,8000,8045,8048,8353,8370,8373,8391,8530,8533,8535,8539,8549,8559,8571,8574,8575,8739,8740,8746,8747,8757,8763,8769,8774,8937,8969,8974,9041,9135,9137,9154,9160,9318,9319,9321,9324,9328,9344,9347,9483,9484,9488,9490,9493,9515,9517,9521,9524,9526,9528,9544,9550,9552,9683,9689,9691,9695,9696,9697,9698,9700,9701,9709,9711,9712,9721,9730,9740,9741,9748,9751,9753,9754,9760,9763,9767,9881,9884,9899,9902,9904,9905,9908,9911,9915,9917,9919,9920,9926,9929,9931,9936,9943,9945,9955,10076,10085,10092,10098,10105,10112,10129,10130,10134,10137,10140,10155,10158,10303,10305,10308,10346,10675,10697,10881,10888,10891,10901,10906,11096,11098,11102,11115,11118,11119,11259,11270,11273,11275,11280,11289,11294,11299,11302,11305,11468,11478,11484,11489,11490,11495,11674,11829,11837,11847,11848,11851,11853,11856,11886,12016,12038,12202,12211,12218,12372,12374,12376,12378,12379,12735,12737,12741,12743,12750,12759,12764,12933,12938,12941,12942,12949,12965,12980,13027,13281,13284,13303,13305,13337,14222,14409,14421,14429,14435,14442,14449,14590,14802,14986,14988,15028,15177,15217,15218,15379,15384,15417,15588,15596,15961,16315,16323,16330,16332,16333,16335,16339,16512,16518,16882,16888,16890,16892,16895,16897,16898,16901,16911,16923,16927,16950,17111,17303,17305,17308,17314,17457,17463,17499,17504,17506,17530,17533,17537,17539,17544,17786,17860,17862,17864,17868,17871,17872,17874,17876,17902,17906,17910,17938,18094,18106,18115,18122,18276,18281,18301,18465,18480,18484,18488,18491,18500,18675,18738,18831,18856,18861,18862,18867,18876,18912,19062,19065,19275,19277,19280,19446,19455,19459,19475,19476,19479,19490,19493,19648,19651,19661,19666,19677,19680,19682,19685,19690,19693,19694,19699,19701,19703,19709,19711,19713,19714,19728,19791,19906,19912,19914,19934,20097,20105,20110,20289,20291,20328,20484,20492,20498,20504,20715,20737,20930,20944,20946,20966,21099,21105,21110,21111,21115,21117,21129,21136,21142,21146,21279,21283,21285,21288,21289,21292,21296,21313,21314,21316,21319,21346,21347,21350,21353,21358,21361,21366,21370,21373,21376,21381,21517,21523,21526,21550,21556,21557,21560,21562,21567,21570,21580,21582,21700,21706,21710,21722,21726,21734,21937,21941,21962,22141,22164,22332,22335,22698,22700,22739,22744,22751,22775,22778,22938,22951,23160,23314,23318,23321,23322,23325,23330,23333,23342,23343,23346,23373,23377,23381,23522,23534,23540,23549,23556,23561,23570,23572,23575,23583,23661,23758,23763,23770,23960,24098,24112,24125,24129,24135,24139,24141,24512,24515,24535,24688,24692,24695,24697,24704,24706,24711,24714,24720,24723,25013,25025,25030,25032,25058,25065,25068,25085,25088,25238,25239,25242,25245,25246,25420,25430,25434,25445,25448,25596,25612,25623,25625,25629,25631,25632,25679,25847,25848,26221,26229,26237,26239,26242,26245,26249,26253,26263,26407,26411,26422,26425,26427,26429,26431,26441,26447,26448,26608,26635,26646,26657,26660,26663,26669,26674,26675,26682,26689,26837,26843,27029,27040,27044,27047,27050,27051,27097,27249,27251,27256,27261,27263,27265,27268,27431,27437,27448,27450,27453,27465,27467,27473,27477,27494,27660,27663,27668,27834,27851,27857,27866, ptg000001l:64052-92038:m54329U_210323_190418/180357787/ccs block 128,0,128,0 Ignored m6A +ptg000001l 64104 91955 5mC 0 - 64104 91955 139,69,19,1 122 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,2,9,429,551,558,760,776,781,870,929,1555,1978,2089,2250,3455,3822,3926,3978,3982,4015,4035,4351,4939,5015,5017,5339,5357,5453,5744,5841,5934,6362,6365,6390,6499,6528,6600,6658,6662,6994,7036,7171,7173,7181,7485,7517,7528,7530,7935,8199,9069,9104,9159,9215,9242,9288,10016,10023,10096,10113,10145,10175,10199,10215,11297,11625,13254,13559,13575,13771,13949,14864,15064,16220,16959,17593,18425,18438,18771,18801,18863,18865,18877,19512,19655,20265,20322,20458,20467,20631,20761,21195,21205,21228,21373,23434,23586,24054,24479,24572,24626,24674,24689,24738,25359,25376,26103,26152,26237,26590,26766,26927,26959,26967,27106,27120,27125,27163,27557,27679,27850, ptg000001l:64052-92038:m54329U_210323_190418/180357787/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 64111 91909 NUC 0 - 64111 91909 169,169,169,1 127 144,141,138,169,164,191,189,163,167,162,136,293,207,205,176,140,146,202,161,165,199,155,162,147,147,197,180,150,147,162,177,151,146,153,159,163,172,135,173,192,304,138,163,162,160,157,135,130,113,120,144,328,183,189,139,162,178,154,129,163,153,355,168,253,884,186,140,211,183,148,160,170,364,353,172,363,160,191,142,241,155,153,163,174,155,149,209,165,154,177,162,178,155,210,192,132,132,135,117,202,178,167,362,159,208,153,140,77,96,189,137,370,152,289,149,173,147,167,372,143,159,147,185,151,162,165,165, 0,195,420,611,798,993,1192,1393,1564,1751,1928,2181,2508,2725,2937,3169,3363,3510,3713,3875,4054,4256,4445,4660,4881,5029,5230,5445,5635,5799,5983,6201,6376,6597,6814,6985,7172,7377,7570,7744,8013,8356,8540,8739,8939,9125,9312,9517,9732,9920,10123,10311,10662,10871,11084,11270,11460,11639,11851,12003,12183,12344,12729,12992,13302,14187,14414,14555,14767,14993,15183,15382,15561,15926,16304,16483,16915,17076,17279,17509,17903,18087,18266,18465,18640,18877,19030,19245,19458,19693,19899,20075,20293,20469,20702,20931,21111,21346,21547,21699,21927,22129,22300,22743,22916,23125,23346,23548,23626,23735,23925,24106,24500,24688,25053,25211,25413,25644,25813,26228,26413,26654,26808,27062,27233,27459,27633, ptg000001l:64052-92038:m54329U_210323_190418/180357787/ccs block 169,169,169,0 Ignored NUC +ptg000001l 64255 91942 LINKER 0 - 64255 91942 147,112,219,1 127 51,84,53,18,31,8,12,8,20,15,117,34,10,7,56,54,1,1,1,14,3,34,53,74,1,4,35,40,17,22,41,24,75,64,12,24,33,58,1,77,39,46,36,38,26,30,70,85,75,83,44,23,26,24,47,28,1,58,23,17,8,30,95,57,1,41,1,1,43,42,39,9,1,25,7,69,1,12,88,153,29,26,36,1,82,4,6,48,81,29,14,40,21,23,37,48,103,66,35,26,24,4,81,14,1,68,62,1,13,1,44,24,36,76,9,29,84,2,43,42,82,7,69,20,64,9,33, 0,192,414,636,818,1040,1237,1412,1587,1769,1920,2330,2571,2786,2969,3165,3365,3568,3730,3896,4109,4267,4463,4663,4884,5082,5266,5451,5638,5817,6016,6208,6378,6606,6829,7004,7200,7368,7599,7792,8173,8350,8559,8757,8955,9138,9303,9503,9701,9896,10123,10495,10701,10916,11079,11288,11494,11649,11836,12022,12192,12555,12753,13101,14042,14229,14410,14622,14806,14997,15199,15408,15781,16135,16332,16702,16931,17123,17277,17606,17914,18096,18285,18495,18651,18882,19095,19266,19468,19726,19917,20109,20304,20535,20750,20919,21099,21337,21520,21757,21961,22152,22518,22758,22980,23134,23342,23481,23578,23780,23918,24332,24508,24833,25058,25240,25416,25667,26041,26227,26428,26657,26849,27069,27251,27480,27654, ptg000001l:64052-92038:m54329U_210323_190418/180357787/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 65781 76510 m6A 0 + 65781 76510 128,0,128,1 1177 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,36,38,41,43,54,55,58,61,62,66,67,70,72,73,80,81,85,88,89,92,96,100,104,108,254,277,279,282,285,290,292,298,449,458,459,461,467,470,473,475,476,478,480,481,484,487,490,492,503,612,619,639,642,646,649,651,652,656,660,662,664,667,669,674,690,693,695,697,719,835,837,844,847,849,852,855,858,862,864,871,872,875,878,880,882,890,893,901,1018,1020,1040,1041,1045,1050,1051,1054,1059,1061,1062,1064,1067,1068,1072,1075,1079,1088,1090,1092,1093,1095,1096,1098,1100,1103,1108,1111,1112,1115,1117,1120,1153,1253,1255,1256,1260,1262,1263,1266,1267,1270,1272,1273,1274,1275,1278,1279,1280,1283,1286,1288,1291,1292,1294,1309,1312,1316,1319,1443,1445,1448,1450,1453,1456,1458,1460,1464,1468,1470,1472,1475,1476,1477,1479,1481,1484,1488,1490,1494,1495,1498,1501,1502,1504,1506,1514,1518,1520,1523,1526,1659,1677,1680,1682,1684,1686,1692,1694,1695,1698,1699,1700,1702,1708,1709,1720,1726,1728,1866,1884,1888,1890,1905,2034,2080,2084,2086,2088,2089,2092,2094,2096,2102,2106,2109,2111,2116,2247,2255,2257,2260,2261,2269,2272,2273,2276,2278,2281,2282,2284,2287,2291,2292,2419,2425,2428,2431,2433,2437,2441,2442,2444,2446,2447,2461,2464,2468,2469,2598,2602,2607,2614,2619,2626,2630,2632,2635,2638,2640,2642,2643,2650,2651,2653,2656,2661,2665,2784,2814,2817,2819,2836,2840,2842,2844,2846,2848,2855,2858,2859,2862,2864,2867,2871,2878,2881,2882,2886,2887,2889,2892,2894,2897,2899,2906,2914,2976,2977,2979,2981,3013,3015,3017,3019,3021,3023,3025,3027,3029,3031,3034,3046,3049,3050,3053,3054,3055,3058,3059,3061,3063,3065,3067,3186,3204,3210,3217,3222,3223,3224,3226,3228,3231,3233,3235,3237,3240,3244,3247,3248,3250,3255,3277,3278,3282,3389,3393,3394,3396,3398,3400,3401,3402,3405,3407,3408,3411,3413,3416,3418,3419,3420,3422,3425,3426,3432,3437,3440,3442,3443,3448,3453,3456,3459,3595,3597,3599,3602,3750,3762,3765,3774,3898,3925,3927,3931,3933,3935,3938,3945,3947,3949,3953,3956,3959,4061,4064,4073,4085,4094,4097,4100,4105,4106,4107,4108,4112,4113,4115,4118,4121,4125,4128,4130,4131,4136,4139,4279,4288,4289,4291,4293,4294,4296,4298,4299,4301,4304,4306,4310,4312,4315,4320,4323,4324,4325,4327,4329,4330,4332,4335,4336,4338,4340,4343,4348,4494,4496,4497,4498,4501,4503,4507,4510,4513,4517,4519,4520,4521,4525,4526,4530,4531,4533,4536,4539,4541,4546,4553,4555,4654,4662,4664,4666,4668,4673,4683,4689,4692,4693,4695,4697,4700,4701,4704,4705,4708,4710,4714,4718,4719,4721,4722,4724,4725,4726,4727,4730,4732,4734,4736,4746,4831,4832,4836,4839,4842,4844,4847,4849,4853,4856,4858,4860,4864,4866,4874,4877,4879,4880,4881,4883,4885,4889,4892,4897,4902,4904,4906,4915,4917,4920,4924,4926,4928,4929,5050,5055,5057,5059,5063,5079,5080,5082,5084,5086,5087,5089,5090,5092,5094,5096,5099,5100,5102,5104,5110,5112,5113,5115,5116,5118,5119,5121,5124,5125,5127,5128,5134,5141,5143,5149,5152,5156,5264,5281,5283,5288,5292,5294,5300,5303,5306,5310,5311,5314,5318,5320,5322,5325,5329,5335,5338,5448,5461,5464,5470,5472,5478,5481,5485,5497,5501,5510,5513,5516,5517,5519,5521,5523,5525,5531,5536,5537,5539,5550,5553,5690,5691,5693,5701,5703,5705,5708,5711,5722,5857,5861,5865,5868,5873,5875,5881,5887,5891,5894,5896,5897,5899,5901,5903,5904,5906,5912,5914,5916,5918,5919,5921,5923,5927,5930,5939,5945,5947,5951,5953,5954,5970,6073,6075,6085,6087,6096,6098,6103,6106,6107,6109,6111,6113,6116,6117,6118,6121,6123,6125,6127,6128,6130,6134,6136,6266,6274,6275,6277,6280,6281,6286,6287,6292,6298,6301,6304,6314,6321,6322,6324,6329,6332,6333,6337,6339,6340,6375,6439,6476,6478,6480,6485,6491,6492,6503,6506,6507,6508,6510,6513,6517,6518,6524,6527,6647,6651,6657,6658,6660,6663,6664,6667,6681,6682,6685,6693,6696,6697,6700,6702,6703,6704,6706,6708,6711,6713,6715,6718,6720,6729,6731,6733,6737,6806,6815,6821,6824,6827,6829,6830,6833,6839,6843,6847,6852,6859,6860,6876,6879,6885,6887,6889,6891,6904,6906,6909,6910,6913,6914,6916,6917,6920,6949,7068,7078,7100,7101,7104,7105,7109,7110,7114,7124,7129,7137,7263,7276,7280,7286,7287,7290,7295,7297,7299,7302,7304,7305,7310,7315,7316,7318,7320,7321,7332,7454,7457,7460,7467,7468,7470,7474,7476,7478,7480,7485,7488,7490,7493,7497,7501,7503,7511,7513,7516,7519,7524,7527,7529,7534,7536,7604,7622,7634,7641,7644,7647,7650,7660,7662,7663,7667,7676,7678,7679,7681,7682,7684,7685,7686,7689,7690,7692,7696,7838,7839,7841,7842,7844,7846,7850,7852,7857,7858,7861,7862,7864,7866,7870,7871,7875,7876,7877,7880,7882,7885,7890,7893,7896,8011,8018,8024,8026,8029,8030,8034,8037,8041,8042,8045,8047,8048,8049,8053,8054,8055,8057,8061,8064,8066,8069,8072,8074,8077,8078,8080,8082,8094,8211,8213,8218,8220,8223,8225,8228,8230,8235,8236,8237,8239,8241,8243,8246,8247,8249,8252,8254,8257,8258,8260,8261,8262,8265,8267,8285,8291,8294,8423,8424,8428,8431,8432,8439,8443,8445,8449,8452,8454,8456,8460,8461,8486,8628,8633,8635,8637,8655,8658,8662,8819,8834,8838,8840,8841,8843,8844,8846,8848,8850,8855,8862,8865,8869,8871,8874,8881,8883,8885,9012,9017,9023,9025,9027,9029,9046,9053,9056,9059,9062,9064,9074,9076,9209,9211,9213,9227,9229,9231,9233,9234,9237,9240,9241,9245,9247,9248,9252,9253,9255,9258,9409,9422,9424,9425,9428,9430,9444,9447,9448,9451,9455,9456,9459,9461,9462,9466,9467,9469,9472,9473,9477,9492,9610,9612,9614,9617,9623,9626,9629,9630,9658,9661,9663,9665,9666,9668,9672,9685,9688,9789,9800,9804,9805,9808,9811,9813,9816,9818,9819,9822,9825,9830,9831,9833,9834,9838,9839,9840,9843,9845,9848,9850,9852,9857,9860,9863,9864,9866,9869,9871,9873,9875,9877,9880,9881,9883,9888,9889,9892,9897,9901,9904,9909,9912,10026,10036,10039,10041,10043,10048,10049,10050,10055,10057,10059,10062,10065,10066,10069,10073,10074,10077,10078,10080,10082,10083,10084,10086,10089,10092,10095,10097,10110,10112,10117,10118,10123,10131,10249,10250,10259,10264,10265,10269,10270,10272,10274,10277,10287,10289,10291,10294,10295,10298,10332,10455,10467,10478,10490,10491,10493,10494,10496,10498,10502,10504,10505,10509,10514,10521,10523,10666,10668,10670,10672,10673,10678,10700,10706,10728, ptg000001l:65740-76562:m54329U_210323_190418/45812047/ccs block 128,0,128,0 Ignored m6A +ptg000001l 66081 75729 5mC 0 + 66081 75729 139,69,19,1 44 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,111,696,1477,1821,1844,1851,1948,2000,2004,2037,2373,2987,3037,3039,3361,3956,4154,4387,4622,4680,4684,5016,5058,5072,5193,5195,5203,5507,5539,5550,5552,5642,6221,7077,7091,7264,7310,7681,8045,8197,8221,8237,9647, ptg000001l:65740-76562:m54329U_210323_190418/45812047/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 65890 76447 NUC 0 + 65890 76447 169,169,169,1 51 145,150,108,115,116,132,123,132,137,128,130,126,128,148,118,106,135,147,150,101,139,145,98,84,120,107,109,136,134,102,129,100,119,147,125,121,141,114,116,128,141,156,126,132,150,117,100,113,117,156,142, 0,190,395,611,793,1012,1211,1418,1620,1797,2008,2184,2361,2557,2959,3174,3351,3494,3666,3851,4031,4240,4447,4638,4821,5048,5230,5445,5614,5862,6028,6267,6419,6812,7029,7224,7588,7788,7986,8186,8378,8554,8777,8968,9150,9384,9580,9804,10023,10190,10415, ptg000001l:65740-76562:m54329U_210323_190418/45812047/ccs block 169,169,169,0 Ignored NUC +ptg000001l 66035 76510 LINKER 0 + 66035 76510 147,112,219,1 51 45,55,108,67,103,67,84,70,40,83,46,51,68,254,97,71,8,25,35,79,70,62,93,99,107,75,106,33,114,64,110,52,274,70,70,243,59,84,84,64,35,67,65,50,84,79,124,106,50,69,63, 0,195,358,581,764,999,1189,1405,1612,1780,1993,2165,2344,2560,2932,3135,3341,3496,3671,3807,4025,4240,4400,4577,4796,5010,5194,5436,5603,5819,6012,6222,6393,6814,7009,7200,7584,7757,7957,8169,8374,8565,8758,8955,9155,9356,9535,9772,9995,10201,10412, ptg000001l:65740-76562:m54329U_210323_190418/45812047/ccs block 147,112,219,0 Ignored LINKER +ptg000001l 70924 81400 m6A 0 - 70924 81400 128,0,128,1 325 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,6,9,13,14,17,20,197,200,203,212,218,225,228,233,374,376,380,382,388,407,410,548,550,554,555,558,560,562,563,565,568,570,572,579,584,590,601,605,610,612,616,758,761,763,776,780,784,787,788,802,804,808,810,811,953,957,960,964,978,980,982,985,1134,1138,1140,1144,1155,1158,1161,1174,1178,1186,1189,1190,1194,1357,1363,1364,1367,1371,1375,1376,1381,1547,1550,1551,1553,1554,1557,1559,1560,1561,1563,1565,1568,1570,1572,1575,1577,1586,1588,1590,1594,1597,1598,1599,1601,1614,1748,1767,1941,1961,1962,1965,1966,1967,2133,2135,2137,2152,2169,2171,2178,2331,2333,2337,2342,2345,2350,2354,2363,2366,2368,2373,2533,2535,2547,2549,2553,2719,2723,2727,2898,2910,2912,2914,2918,2923,2926,2929,3085,3087,3092,3094,3098,3103,3104,3106,3109,3111,3115,3118,3121,3142,3148,3151,3302,3306,3309,3311,3317,3328,3332,3334,3337,3338,3341,3343,3345,3353,3512,3515,3519,3522,3525,3526,3545,3547,3549,3551,3556,3558,3723,3728,3731,3740,3742,3913,3916,3919,3931,4298,4304,4308,4313,4316,4440,4691,4705,4707,4717,4720,4723,4726,4728,4730,4734,4738,4749,4750,4754,4761,4919,4943,5129,5148,5152,5167,5314,5324,5351,5366,5378,5380,5391,5394,5400,5557,5581,5585,5736,5742,5745,5748,5750,5753,5756,5759,5927,5930,5934,6097,6099,6114,6123,6131,6888,7362,7462,7482,7495,7517,7674,7675,7689,7696,7874,7876,7878,7879,7882,7885,7900,8057,8060,8064,8066,8075,8077,8080,8087,8259,8265,8277,8461,8745,8999,9010,9152,9156,9161,9165,9313,9316,9319,9331,9338,9383,9512,9522,9537,9541,9544,9546,9548,9551,9552,9556,9572,9725,9735,9736,9737,9916,10103,10105,10278,10281,10284,10304,10448,10450,10456,10475, ptg000001l:70845-81453:m54329U_210323_190418/9503691/ccs block 128,0,128,0 Ignored m6A +ptg000001l 71098 80325 5mC 0 - 71098 80325 139,69,19,1 35 1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, 0,177,179,187,310,429,491,502,534,536,562,626,951,1205,1524,2110,2127,2165,2221,2665,3022,3029,3181,3205,4485,4631,5758,6260,6565,6581,6777,6955,8070,8768,9226, ptg000001l:70845-81453:m54329U_210323_190418/9503691/ccs block 139,69,19,0 Ignored 5mC +ptg000001l 70945 81372 NUC 0 - 70945 81372 169,169,169,1 48 176,140,137,141,141,148,162,165,133,173,165,152,159,165,170,155,150,158,164,170,366,123,250,157,185,146,156,150,167,162,756,473,99,156,177,156,171,183,283,253,141,147,128,152,178,186,172,143, 0,213,390,596,791,965,1174,1361,1594,1747,1947,2158,2353,2533,2707,2909,3131,3333,3538,3722,3911,4296,4420,4741,4923,5147,5380,5565,5739,5914,6111,6868,7342,7497,7676,7880,8067,8257,8441,8725,8990,9145,9363,9552,9717,9896,10085,10284, ptg000001l:70845-81453:m54329U_210323_190418/9503691/ccs block 169,169,169,0 Ignored NUC +ptg000001l 70924 81400 LINKER 0 - 70924 81400 147,112,219,1 49 21,37,37,69,54,33,61,25,68,20,27,46,43,21,9,32,67,52,47,20,19,19,1,71,25,39,87,29,24,8,35,1,1,56,23,27,31,19,1,1,12,14,71,61,13,1,3,27,28, 0,197,374,548,758,953,1134,1357,1547,1748,1941,2133,2331,2533,2719,2898,3085,3302,3512,3723,3913,4298,4440,4691,4919,5129,5314,5557,5736,5927,6097,6888,7362,7462,7674,7874,8057,8259,8461,8745,8999,9152,9313,9512,9725,9916,10103,10278,10448, ptg000001l:70845-81453:m54329U_210323_190418/9503691/ccs block 147,112,219,0 Ignored LINKER diff --git a/tests/regression/snapshots/regression__fire__fire_extract_reports_fire_fdrs.snap b/tests/regression/snapshots/regression__fire__fire_extract_reports_fire_fdrs.snap new file mode 100644 index 00000000..daafc38f --- /dev/null +++ b/tests/regression/snapshots/regression__fire__fire_extract_reports_fire_fdrs.snap @@ -0,0 +1,25 @@ +--- +source: tests/regression/fire.rs +expression: fire_rows +--- +ptg000001l 1653 1817 m54329U_210323_190418/5048829/ccs 4 - 1653 1817 225,0,0 0.0392157 UNK +ptg000001l 23315 23481 m54329U_210323_190418/32113767/ccs 2 - 23315 23481 175,0,0 0.015686264 UNK +ptg000001l 30867 31014 m54329U_210323_190418/32113767/ccs 4 - 30867 31014 225,0,0 0.0392157 UNK +ptg000001l 38220 38416 m54329U_210323_190418/66718332/ccs 5 + 38220 38416 255,0,0 0.04705879 UNK +ptg000001l 25261 25413 m54329U_210323_190418/90833781/ccs 3 - 25261 25413 200,0,0 0.027450943 UNK +ptg000001l 29769 29881 m54329U_210323_190418/90833781/ccs 5 - 29769 29881 255,0,0 0.04705879 UNK +ptg000001l 26088 26305 m54329U_210323_190418/85459034/ccs 1 + 26088 26305 175,0,0 0.011764679 UNK +ptg000001l 27744 28045 m54329U_210323_190418/85459034/ccs 5 + 27744 28045 255,0,0 0.04705879 UNK +ptg000001l 33916 34061 m54329U_210323_190418/85459034/ccs 5 + 33916 34061 255,140,0 0.050980378 UNK +ptg000001l 36368 36458 m54329U_210323_190418/10290844/ccs 5 - 36368 36458 255,140,0 0.050980378 UNK +ptg000001l 36630 36784 m54329U_210323_190418/2491749/ccs 4 - 36630 36784 255,0,0 0.043137282 UNK +ptg000001l 40171 40361 m54329U_210323_190418/2491749/ccs 2 - 40171 40361 200,0,0 0.023529435 UNK +ptg000001l 41415 41503 m54329U_210323_190418/94437983/ccs 5 - 41415 41503 255,0,0 0.04705879 UNK +ptg000001l 59160 59295 m54329U_210323_190418/143787994/ccs 4 - 59160 59295 225,0,0 0.0392157 UNK +ptg000001l 57317 57469 m54329U_210323_190418/98239231/ccs 5 + 57317 57469 255,0,0 0.04705879 UNK +ptg000001l 63441 63542 m54329U_210323_190418/98239231/ccs 5 + 63441 63542 255,0,0 0.04705879 UNK +ptg000001l 65035 65173 m54329U_210323_190418/98239231/ccs 3 + 65035 65173 225,0,0 0.03137253 UNK +ptg000001l 63977 64153 m54329U_210323_190418/54199156/ccs 5 - 63977 64153 255,0,0 0.04705879 UNK +ptg000001l 58646 58826 m54329U_210323_190418/111871348/ccs 3 + 58646 58826 225,0,0 0.03137253 UNK +ptg000001l 68719 69064 m54329U_210323_190418/111871348/ccs 4 + 68719 69064 225,0,0 0.0392157 UNK +ptg000001l 73171 73532 m54329U_210323_190418/14615259/ccs 4 + 73171 73532 255,0,0 0.043137282 UNK diff --git a/tests/regression/snapshots/regression__qc__qc_m6a_per_msp_sees_fire_elements.snap b/tests/regression/snapshots/regression__qc__qc_m6a_per_msp_sees_fire_elements.snap new file mode 100644 index 00000000..53d9c869 --- /dev/null +++ b/tests/regression/snapshots/regression__qc__qc_m6a_per_msp_sees_fire_elements.snap @@ -0,0 +1,25 @@ +--- +source: tests/regression/qc.rs +expression: "fire_rows.join(\"\\n\")" +--- +m6a_per_msp_size 14,197,true 1 +m6a_per_msp_size 22,88,true 1 +m6a_per_msp_size 29,90,true 1 +m6a_per_msp_size 36,136,true 1 +m6a_per_msp_size 37,103,true 1 +m6a_per_msp_size 37,190,true 1 +m6a_per_msp_size 42,112,true 1 +m6a_per_msp_size 46,147,true 1 +m6a_per_msp_size 46,153,true 1 +m6a_per_msp_size 47,166,true 1 +m6a_per_msp_size 49,138,true 1 +m6a_per_msp_size 57,145,true 1 +m6a_per_msp_size 57,165,true 1 +m6a_per_msp_size 59,177,true 1 +m6a_per_msp_size 60,154,true 1 +m6a_per_msp_size 61,152,true 1 +m6a_per_msp_size 62,182,true 1 +m6a_per_msp_size 75,300,true 1 +m6a_per_msp_size 91,353,true 1 +m6a_per_msp_size 93,218,true 1 +m6a_per_msp_size 107,362,true 1 From 7e326e67b617e241655d3e9a07c0570ae8aefa93 Mon Sep 17 00:00:00 2001 From: "Mitchell R. Vollger" Date: Thu, 13 Aug 2026 10:17:01 -0700 Subject: [PATCH 2/5] =?UTF-8?q?fix:=20more=20MA-refactor=20fallout=20?= =?UTF-8?q?=E2=80=94=20filter=20expressions,=20mock-fire,=20stale=20legacy?= =?UTF-8?q?=20tags?= MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Three more instances of the 0.10 MA-refactor bug class, found by a systematic sweep: - -x 'qual(msp)' compared against MSP quals, which are always 0 post-MA (the fire-qual path was gated on fire count == msp count, but fire is the p>0 strict subset). '>N' dropped everything including called FIREs; ' --- src/subcommands/convert_tags.rs | 21 ++---- src/subcommands/mock_fire.rs | 8 +- src/utils/ftexpression.rs | 95 +++++++++++++----------- src/utils/ma_io.rs | 123 ++++++++++++++++++++++++++++--- tests/regression.rs | 2 + tests/regression/convert_tags.rs | 48 ++++++++++++ tests/regression/fire.rs | 72 ++++++++++++++++++ tests/regression/mock_fire.rs | 37 ++++++++++ 8 files changed, 336 insertions(+), 70 deletions(-) create mode 100644 tests/regression/mock_fire.rs diff --git a/src/subcommands/convert_tags.rs b/src/subcommands/convert_tags.rs index 89f7889c..76060d79 100644 --- a/src/subcommands/convert_tags.rs +++ b/src/subcommands/convert_tags.rs @@ -12,11 +12,14 @@ use rust_htslib::bam::Read; /// dropped on the way in (the legacy `aq` byte becomes the separate `fire` /// type), so converted MSP tags carry no qualities. /// -/// Legacy tags are removed **only** when they were the source of the -/// annotations, i.e. the record had no `MA` tag. A record that already carries -/// an `MA` tag is passed through untouched, so we never strip identically-named -/// aux tags written by another tool. `MM`/`ML` are preserved byte-identically -/// (this path does not touch base modifications). +/// Legacy tags (including the fibertig `fs`/`fl`/`fa` set) are removed +/// **only** when they were the source of the annotations, i.e. the record had +/// no `MA` tag — this provenance rule is enforced by `ma_io::write_record` +/// itself, shared with every other MA-writing subcommand. A record that +/// already carries an `MA` tag keeps its legacy-named aux tags untouched, so +/// we never strip identically-named tags written by another tool. `MM`/`ML` +/// are preserved byte-identically (this path does not touch base +/// modifications). pub fn convert_tags(opts: &mut cli::ConvertTagsOptions) { let mut bam = opts.input.bam_reader(); let mut out = opts.input.bam_writer(&opts.out); @@ -24,17 +27,9 @@ pub fn convert_tags(opts: &mut cli::ConvertTagsOptions) { for rec in bam.records() { let mut record = rec.expect("failed to read BAM record"); - let had_ma = record.aux(b"MA").is_ok(); let annot = ma_io::read_record(&record) .unwrap_or_else(|e| panic!("failed to read annotations: {e}")); - // Provenance guard: only remove legacy tags we actually consumed. - if !had_ma { - for tag in ma_io::LEGACY_READ_TAGS { - record.remove_aux(tag).ok(); - } - } - ma_io::write_record(&mut record, &annot); out.write(&record).expect("failed to write BAM record"); bar.inc(1); diff --git a/src/subcommands/mock_fire.rs b/src/subcommands/mock_fire.rs index 37412b86..9bf2c2a0 100644 --- a/src/subcommands/mock_fire.rs +++ b/src/subcommands/mock_fire.rs @@ -129,13 +129,17 @@ fn create_mock_fire_record( quals.push(quality); } - // Build MA-spec annotations and emit. Mock FIRE only produces MSPs with - // the FIRE precision (Q-scaled) quality; no nucleosomes are populated. + // Build MA-spec annotations and emit. No nucleosomes are populated. + // Real fire-scored records carry every interval as an `msp` annotation + // (no quals) with the called subset duplicated into the `fire` type, so + // emit both — fire-only records are invisible to msp-driven consumers + // (pileup msp_coverage, ft extract, qc, filter expressions). // Using `ma_io::write_record_with_basemods` directly (rather than the // `FiberseqData::serialize_annotations` path used elsewhere) is intentional // here — we're synthesizing a record from BED, so there's no `FiberseqData` // to round-trip through. let mut annot = MolecularAnnotations::from_record(&record); + ma_io::add_msp_annotations(&mut annot, &starts, &lengths, None); ma_io::add_fire_annotations(&mut annot, &starts, &lengths, &quals); ma_io::write_record_with_basemods(&mut record, &annot); diff --git a/src/utils/ftexpression.rs b/src/utils/ftexpression.rs index da4a6007..1caf9672 100644 --- a/src/utils/ftexpression.rs +++ b/src/utils/ftexpression.rs @@ -150,55 +150,62 @@ pub fn apply_filter_fsd(fsd: &mut FiberseqData, filt: &FiberFilters) -> Result<( other => anyhow::bail!("Unknown feature name: {}", other), }; match parser.fn_name.as_str() { - "len" => fsd.annotations.retain(type_name, |a| { - len(a.length as i64, &parser.op, &parser.threshold) - }), + "len" => { + fsd.annotations.retain(type_name, |a| { + len(a.length as i64, &parser.op, &parser.threshold) + }); + // fire annotations are interval copies of their source + // MSPs — apply the same predicate so they are not + // orphaned when their parent MSP is removed. + if type_name == "msp" { + fsd.annotations.retain("fire", |a| { + len(a.length as i64, &parser.op, &parser.threshold) + }); + } + } "qual" if type_name == "msp" => { // qual(msp) historically meant "filter MSPs by FIRE // precision" (legacy fibertools wrote FIRE precisions // onto the MSP `aq` tag). Post-MA, that quality lives - // on the `fire` annotation type instead. Collect - // per-MSP precisions in *molecular* order to align - // with retain's iteration, then drop msp and fire - // in lockstep. + // on the `fire` annotation type, which holds the p>0 + // SUBSET of MSPs as exact interval copies. Overlay the + // fire quals onto the MSPs keyed by molecular start; + // MSPs without a fire entry fall back to their own + // qual (legacy read path) and so evaluate as 0. Drop + // msp and fire entries by the same kept-start set so + // the two types stay paired. let primary = crate::utils::bamannotations::primary_qual; - let mol_quals: Vec = if let Some(f) = - fsd.annotations.get_type("fire").filter(|f| { - fsd.annotations - .get_type("msp") - .is_some_and(|m| m.annotations.len() == f.annotations.len()) - }) { - f.annotations - .iter() - .map(|a| primary(&a.qualities, "fire")) - .collect() - } else if let Some(m) = fsd.annotations.get_type("msp") { - m.annotations - .iter() - .map(|a| primary(&a.qualities, "msp")) - .collect() - } else { - Vec::new() - }; - let keep: Vec = mol_quals - .iter() - .map(|q| qual(*q, &parser.op, &parser.threshold)) - .collect(); - let has_fire = fsd.annotations.get_type("fire").is_some(); - let mut i = 0; - fsd.annotations.retain("msp", |_| { - let k = keep.get(i).copied().unwrap_or(false); - i += 1; - k - }); - if has_fire { - let mut i = 0; - fsd.annotations.retain("fire", |_| { - let k = keep.get(i).copied().unwrap_or(false); - i += 1; - k - }); - } + let fire_quals: std::collections::HashMap = fsd + .annotations + .get_type("fire") + .map(|f| { + f.annotations + .iter() + .map(|a| (a.start, primary(&a.qualities, "fire"))) + .collect() + }) + .unwrap_or_default(); + let keep_starts: std::collections::HashSet = fsd + .annotations + .get_type("msp") + .map(|m| { + m.annotations + .iter() + .filter(|a| { + let q = fire_quals + .get(&a.start) + .copied() + .unwrap_or_else(|| primary(&a.qualities, "msp")); + qual(q, &parser.op, &parser.threshold) + }) + .map(|a| a.start) + .collect() + }) + .unwrap_or_default(); + fsd.annotations + .retain("msp", |a| keep_starts.contains(&a.start)); + fsd.annotations + .retain("fire", |a| keep_starts.contains(&a.start)); } "qual" => fsd.annotations.retain(type_name, |a| { qual( diff --git a/src/utils/ma_io.rs b/src/utils/ma_io.rs index e1fb944a..8113f427 100644 --- a/src/utils/ma_io.rs +++ b/src/utils/ma_io.rs @@ -34,6 +34,11 @@ pub const FIRE_TYPE: &str = "fire"; /// MA tag is present. These are never emitted; we only ingest them. pub const LEGACY_READ_TAGS: &[&[u8]] = &[b"ns", b"nl", b"as", b"al", b"aq"]; +/// Legacy fibertig tags, likewise consumed only as a read fallback (see +/// [`read_legacy_fibertig`]) and stripped alongside [`LEGACY_READ_TAGS`] +/// when they were the source of a written annotation model. +pub const LEGACY_FIBERTIG_TAGS: &[&[u8]] = &[b"fs", b"fl", b"fa"]; + /// Molecular-orientation nuc/msp arrays returned by [`extract_nuc_msp_arrays`]: /// `(nuc_starts, nuc_lengths, msp_starts, msp_lengths, msp_qual)`. `msp_qual` /// is empty when there are no MSP qualities (pre-FIRE state). @@ -59,20 +64,19 @@ pub fn read_record(record: &bam::Record) -> Result { // legacy fallback whenever MM/ML is present). let has_ma = matches!(record.aux(b"MA"), Ok(Aux::String(_))); if !has_ma { - // Provenance caveat: presence of `ns`/`as`/`fs` is treated as evidence - // of fibertools-legacy data with no further validation. This path is - // read-only — we never delete these tags — so a foreign tool reusing - // those names is at worst mis-parsed here, never destroyed. Any future - // code that *removes* legacy tags must verify provenance first. - let has_legacy_nuc = record.aux(b"ns").is_ok(); - let has_legacy_msp = record.aux(b"as").is_ok(); - if has_legacy_nuc || has_legacy_msp { + // Provenance: the gates are type-checked (`has_legacy_nuc_msp`, + // `has_legacy_fibertig`), so a foreign tool reusing these two-letter + // names with a different aux type is never parsed as fibertools data. + // The write path (`strip_consumed_legacy_tags`) removes a legacy tag + // only under these same gates plus a per-tag type check — i.e. only + // when this reader consumed it — so keep the two in sync. + if has_legacy_nuc_msp(record) { merge_missing_types(&mut annot, read_legacy_nuc_msp(record)?); } // Legacy fibertig (`fs`/`fl`/`fa`): the pre-MA fibertig wire format, // dropped from the writer in favour of the MA-spec `AN` tag. Kept - // read-only so older fibertig BAMs stay consumable by `extract`. - if record.aux(b"fs").is_ok() { + // readable so older fibertig BAMs stay consumable by `extract`. + if has_legacy_fibertig(record) { merge_missing_types(&mut annot, read_legacy_fibertig(record)?); } } @@ -133,9 +137,68 @@ fn merge_missing_types(dst: &mut MolecularAnnotations, src: MolecularAnnotations /// Producers that create or modify base mods must instead call /// [`write_record_with_basemods`], which canonically re-emits MM/ML. pub fn write_record(record: &mut bam::Record, annot: &MolecularAnnotations) { + strip_consumed_legacy_tags(record); annot.to_record(record); } +/// Provenance rule shared by every MA write: strip exactly the legacy tag +/// sets that [`read_record`] consumed as the source of the annotation model +/// being written — they are superseded by the MA tags (v0.9 replace +/// semantics; otherwise legacy readers silently see stale calls forever). +/// +/// The gates mirror [`read_record`]'s consumption gates, which is what makes +/// this provenance-safe: a set is only removed when the reader ingested it +/// (and it parsed as fibertools data — a parse failure errors out before any +/// write). Records that already carry `MA` were not read from legacy tags, +/// so their legacy-named aux tags are left untouched: a foreign tool reusing +/// those names is never destroyed. Likewise a lone foreign tag outside its +/// set's gate (e.g. `nl` with no `ns`/`as`) is left alone. +fn strip_consumed_legacy_tags(record: &mut bam::Record) { + if matches!(record.aux(b"MA"), Ok(Aux::String(_))) { + return; + } + if has_legacy_nuc_msp(record) { + for tag in LEGACY_READ_TAGS { + if is_legacy_typed(record, tag) { + record.remove_aux(tag).ok(); + } + } + } + if has_legacy_fibertig(record) { + for tag in LEGACY_FIBERTIG_TAGS { + if is_legacy_typed(record, tag) { + record.remove_aux(tag).ok(); + } + } + } +} + +/// Type-checked consumption gate for the legacy nuc/msp tag set. Presence +/// alone is weak provenance — a foreign tool could reuse these two-letter +/// names with a different aux type — so the set only counts as +/// fibertools-legacy when a gate tag parses as the int array legacy +/// fibertools wrote. +fn has_legacy_nuc_msp(record: &bam::Record) -> bool { + u32_array(record, b"ns").is_some() || u32_array(record, b"as").is_some() +} + +/// Type-checked consumption gate for the legacy fibertig tag set. +fn has_legacy_fibertig(record: &bam::Record) -> bool { + u32_array(record, b"fs").is_some() && u32_array(record, b"fl").is_some() +} + +/// True when `tag` carries the aux type legacy fibertools wrote for it — +/// the per-tag half of the provenance check in +/// [`strip_consumed_legacy_tags`]: even inside a gated set, a wrong-typed +/// tag was not consumed by the reader and so is never removed. +fn is_legacy_typed(record: &bam::Record, tag: &[u8]) -> bool { + match tag { + b"aq" => u8_array(record, tag).is_some(), + b"fa" => matches!(record.aux(tag), Ok(Aux::String(_))), + _ => u32_array(record, tag).is_some(), + } +} + /// Writes MA-family tags **and** canonically re-encodes MM/ML from the /// annotation model. /// @@ -151,7 +214,7 @@ pub fn write_record(record: &mut bam::Record, annot: &MolecularAnnotations) { /// Basemod types must already be `Encoding::MmMl` — they are when read from a /// record, and producers create them that way (e.g. `Encoding::mm_ml()`). pub fn write_record_with_basemods(record: &mut bam::Record, annot: &MolecularAnnotations) { - annot.to_record(record); + write_record(record, annot); annot.write_mm_ml(record); } @@ -495,6 +558,44 @@ mod tests { use super::*; use molecular_annotation::{MolecularAnnotations, QualitySpec, Strand}; + /// Foreign tags that merely reuse legacy names with a different aux type + /// must never be stripped by the MA write path; correctly-typed consumed + /// legacy tags must be. + #[test] + fn strip_consumed_legacy_tags_checks_types() { + // foreign: `ns` as a string, `al` as a float — not fibertools-typed + let mut foreign = synth_record(b"ACGTACGT"); + foreign.push_aux(b"ns", Aux::String("not-ours")).unwrap(); + foreign.push_aux(b"al", Aux::Float(1.5)).unwrap(); + strip_consumed_legacy_tags(&mut foreign); + assert!(foreign.aux(b"ns").is_ok(), "foreign string ns was stripped"); + assert!(foreign.aux(b"al").is_ok(), "foreign float al was stripped"); + + // fibertools-typed legacy nuc/msp set: consumed, so stripped — but a + // wrong-typed member of the set (string aq) survives + let mut legacy = synth_record(b"ACGTACGT"); + legacy + .push_aux(b"ns", Aux::ArrayU32((&vec![1u32, 5]).into())) + .unwrap(); + legacy + .push_aux(b"nl", Aux::ArrayU32((&vec![2u32, 2]).into())) + .unwrap(); + legacy.push_aux(b"aq", Aux::String("not-ours")).unwrap(); + strip_consumed_legacy_tags(&mut legacy); + assert!(legacy.aux(b"ns").is_err(), "consumed ns not stripped"); + assert!(legacy.aux(b"nl").is_err(), "consumed nl not stripped"); + assert!(legacy.aux(b"aq").is_ok(), "wrong-typed aq was stripped"); + + // a record already carrying MA keeps even fibertools-typed legacy tags + let mut with_ma = synth_record(b"ACGTACGT"); + with_ma.push_aux(b"MA", Aux::String("8;")).unwrap(); + with_ma + .push_aux(b"ns", Aux::ArrayU32((&vec![1u32]).into())) + .unwrap(); + strip_consumed_legacy_tags(&mut with_ma); + assert!(with_ma.aux(b"ns").is_ok(), "legacy tag stripped despite MA"); + } + /// Build a synthetic record with the given forward sequence. Aligned-blocks /// metadata is not needed for these I/O tests because we only exercise /// tag-level round-trips, not coordinate liftovers. diff --git a/tests/regression.rs b/tests/regression.rs index ffcb41be..43eb9467 100644 --- a/tests/regression.rs +++ b/tests/regression.rs @@ -12,6 +12,8 @@ mod extract; mod fire; #[path = "regression/footprint.rs"] mod footprint; +#[path = "regression/mock_fire.rs"] +mod mock_fire; #[path = "regression/pileup.rs"] mod pileup; #[path = "regression/predict_m6a.rs"] diff --git a/tests/regression/convert_tags.rs b/tests/regression/convert_tags.rs index 5aa46b16..bdeb16cb 100644 --- a/tests/regression/convert_tags.rs +++ b/tests/regression/convert_tags.rs @@ -96,3 +96,51 @@ fn convert_tags_is_idempotent() { } } } + +// Legacy fibertig fs/fl/fa tags are consumed by the reader (into the MA +// fibertig type) and must be stripped on conversion just like ns/nl/as/al/aq +// — otherwise the source tags survive as duplicate, potentially-stale copies. +#[test] +fn convert_tags_strips_consumed_fibertig_tags() { + use rust_htslib::bam::Header; + + // synthesize a legacy fibertig BAM from the first msp_nuc.bam record + let input = fixture("msp_nuc.bam"); + let mut reader = bam::Reader::from_path(&input).unwrap(); + let header = Header::from_template(reader.header()); + let synth = NamedTempFile::with_suffix(".bam").unwrap(); + { + let mut writer = bam::Writer::from_path(synth.path(), &header, bam::Format::Bam).unwrap(); + let mut rec = reader.records().next().unwrap().unwrap(); + rec.push_aux(b"fs", Aux::ArrayU32((&vec![100u32, 500]).into())) + .unwrap(); + rec.push_aux(b"fl", Aux::ArrayU32((&vec![50u32, 60]).into())) + .unwrap(); + rec.push_aux(b"fa", Aux::String("gene_a|")).unwrap(); + writer.write(&rec).unwrap(); + } + + let out = NamedTempFile::with_suffix(".bam").unwrap(); + convert(synth.path(), out.path()); + let rec = &records(out.path())[0]; + for tag in [b"fs", b"fl", b"fa"] { + assert!( + rec.aux(tag).is_err(), + "fibertig tag {} survived conversion", + String::from_utf8_lossy(tag) + ); + } + let ma_tag = ma(rec).expect("MA tag not written"); + assert!( + ma_tag.contains("fibertig"), + "fibertig annotations not carried into MA tag: {ma_tag}" + ); + let an = match rec.aux(b"AN") { + Ok(Aux::String(s)) => s.to_string(), + _ => String::new(), + }; + assert!( + an.contains("gene_a"), + "fibertig name not carried into AN tag: {an}" + ); +} diff --git a/tests/regression/fire.rs b/tests/regression/fire.rs index 554112b9..631394ef 100644 --- a/tests/regression/fire.rs +++ b/tests/regression/fire.rs @@ -1,6 +1,78 @@ use super::common::{fixture, run, select_tsv_cols}; +use rust_htslib::bam::{self, Read}; use tempfile::NamedTempFile; +fn extract_fdrs(out: &str) -> Vec { + out.lines() + .map(|l| l.split('\t').nth(9).unwrap().parse().unwrap()) + .collect() +} + +// -x "qual(msp)" historically filtered MSPs by FIRE precision (legacy aq +// tag). Post-MA the precision lives on the `fire` type, so the filter must +// overlay fire quals onto MSPs: > keeps the called FIREs, < drops them, and +// the fire type must never be orphaned from its parent MSPs. +#[test] +fn filter_expression_qual_msp_uses_fire_quals() { + let scored = NamedTempFile::with_suffix(".bam").unwrap(); + run(&[ + "fire", + fixture("all.bam").to_str().unwrap(), + scored.path().to_str().unwrap(), + ]); + let above = run(&[ + "fire", + "--extract", + "-x", + "qual(msp)>100", + scored.path().to_str().unwrap(), + ]); + let fdrs = extract_fdrs(&above); + assert!(!fdrs.is_empty(), "qual(msp)>100 kept no MSPs"); + assert!( + fdrs.iter().all(|&f| f < 1.0), + "qual(msp)>100 kept non-FIRE MSPs" + ); + let below = run(&[ + "fire", + "--extract", + "-x", + "qual(msp)<100", + scored.path().to_str().unwrap(), + ]); + let fdrs = extract_fdrs(&below); + assert!(!fdrs.is_empty(), "qual(msp)<100 kept no MSPs"); + assert!( + fdrs.iter().all(|&f| f >= 1.0), + "qual(msp)<100 kept called FIRE elements" + ); +} + +// `ft fire` on a legacy-tag BAM consumes ns/nl/as/al/aq and writes MA tags; +// the consumed legacy tags must be stripped (v0.9 replace semantics) or +// legacy readers silently see stale calls forever. +#[test] +fn fire_on_legacy_input_strips_consumed_legacy_tags() { + let scored = NamedTempFile::with_suffix(".bam").unwrap(); + run(&[ + "fire", + fixture("msp_nuc.bam").to_str().unwrap(), + scored.path().to_str().unwrap(), + ]); + let mut reader = bam::Reader::from_path(scored.path()).unwrap(); + for rec in reader.records() { + let rec = rec.unwrap(); + assert!(rec.aux(b"MA").is_ok(), "record missing MA tag"); + for tag in [b"ns", b"nl", b"as", b"al", b"aq"] { + assert!( + rec.aux(tag).is_err(), + "stale legacy tag {} left on fire output", + String::from_utf8_lossy(tag) + ); + } + } +} + // `ft fire` stores FIRE calls on the `fire` annotation type (MA spec); the // MSPs themselves carry no quals. `--extract` must overlay the fire quals // onto the matching MSPs, otherwise every row comes out with FDR = 1.0 and diff --git a/tests/regression/mock_fire.rs b/tests/regression/mock_fire.rs new file mode 100644 index 00000000..69952e00 --- /dev/null +++ b/tests/regression/mock_fire.rs @@ -0,0 +1,37 @@ +use super::common::run; +use rust_htslib::bam::record::Aux; +use rust_htslib::bam::{self, Read}; +use tempfile::NamedTempFile; + +// Real fire-scored records carry every interval as an `msp` annotation with +// the called subset duplicated into the `fire` type. mock-fire must emit +// both, or its output is invisible to msp-driven consumers (pileup +// msp_coverage, extract, qc, filter expressions). +#[test] +fn mock_fire_emits_msp_and_fire_annotations() { + let bed = NamedTempFile::with_suffix(".bed").unwrap(); + std::fs::write( + bed.path(), + "chr1\t100\t200\tread1\t90\nchr1\t400\t500\tread1\t80\n", + ) + .unwrap(); + let out = NamedTempFile::with_suffix(".bam").unwrap(); + run(&[ + "mock-fire", + bed.path().to_str().unwrap(), + "-o", + out.path().to_str().unwrap(), + ]); + let mut reader = bam::Reader::from_path(out.path()).unwrap(); + for rec in reader.records() { + let rec = rec.unwrap(); + let Ok(Aux::String(ma)) = rec.aux(b"MA") else { + panic!("mock-fire record has no MA tag"); + }; + assert!(ma.contains("msp"), "mock-fire MA tag lacks msp group: {ma}"); + assert!( + ma.contains("fire"), + "mock-fire MA tag lacks fire group: {ma}" + ); + } +} From 279d03e7be31c4a74dc4844a5ff0283040250b35 Mon Sep 17 00:00:00 2001 From: "Mitchell R. Vollger" Date: Thu, 13 Aug 2026 10:21:43 -0700 Subject: [PATCH 3/5] docs: AL tag is not part of the MA write set (lengths are inline in MA:Z) Co-Authored-By: Claude Fable 5 --- src/utils/ma_io.rs | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/utils/ma_io.rs b/src/utils/ma_io.rs index 8113f427..0e3022d2 100644 --- a/src/utils/ma_io.rs +++ b/src/utils/ma_io.rs @@ -100,7 +100,7 @@ fn merge_missing_types(dst: &mut MolecularAnnotations, src: MolecularAnnotations } } -/// Writes MA-family tags (MA/AL/AQ/AN) to a BAM record, **preserving the +/// Writes MA-family tags (MA/AQ/AN) to a BAM record, **preserving the /// record's existing MM/ML bytes**. /// /// # Which write function do I call? From e6aa8b84d6c6849038836a14c59586bda31a7740 Mon Sep 17 00:00:00 2001 From: "Mitchell R. Vollger" Date: Thu, 13 Aug 2026 10:23:20 -0700 Subject: [PATCH 4/5] docs: retire stale AL / separate-lengths references (lengths are inline in MA:Z) Co-Authored-By: Claude Fable 5 --- molecular-annotation/README.md | 7 +++---- molecular-annotation/docs/mm-ml-per-group-passthrough.md | 2 +- molecular-annotation/examples/fiberseq_to_ma.rs | 5 +---- molecular-annotation/src/lib.rs | 2 +- 4 files changed, 6 insertions(+), 10 deletions(-) diff --git a/molecular-annotation/README.md b/molecular-annotation/README.md index fa0738cc..72c9df81 100644 --- a/molecular-annotation/README.md +++ b/molecular-annotation/README.md @@ -16,10 +16,9 @@ molecular-annotation = { path = ".", features = ["htslib"] } ## Encoding Formats -Two encoding formats are available via feature flags: - -- **`inline-lengths`** (default): Lengths in MA string: `MA:Z:1000;nuc+:100-50,200-60` -- **`separate-lengths`**: Lengths in AL array: `MA:Z:1000;nuc+:100,200` + `AL:B:I,50,60` +Lengths are encoded inline in the MA string (`MA:Z:1000;nuc+:100-50,200-60`). +The retired separate-length encoding (`AL` array) is stripped on write and +never emitted. ## Documentation diff --git a/molecular-annotation/docs/mm-ml-per-group-passthrough.md b/molecular-annotation/docs/mm-ml-per-group-passthrough.md index 93fd6495..785b5f71 100644 --- a/molecular-annotation/docs/mm-ml-per-group-passthrough.md +++ b/molecular-annotation/docs/mm-ml-per-group-passthrough.md @@ -12,7 +12,7 @@ but non-canonical encodings (grouped multi-code `C+mh`, `N+a` wildcards). Today we work around this with two write functions, and the caller picks based on intent: -- `write_record` / `to_record` — writes only MA-family tags (`MA`/`AL`/`AQ`/`AN`), +- `write_record` / `to_record` — writes only MA-family tags (`MA`/`AQ`/`AN`), leaves the record's MM/ML bytes untouched. Used by structural editors (fire, add-nucs, footprint, pileup, extract, center, convert-tags) via `FiberseqData::serialize_annotations`. diff --git a/molecular-annotation/examples/fiberseq_to_ma.rs b/molecular-annotation/examples/fiberseq_to_ma.rs index e61c98a2..e7a217c1 100644 --- a/molecular-annotation/examples/fiberseq_to_ma.rs +++ b/molecular-annotation/examples/fiberseq_to_ma.rs @@ -1,10 +1,7 @@ //! Convert fiber-seq BAM tags (ns/nl, as/al/aq) to MolecularAnnotations format //! //! This example reads a CRAM/BAM file with fiber-seq annotations and converts -//! them to the MA/AQ tag format (inline lengths, the default). -//! -//! It also writes M2/AL tags with the alternative separate format for compression -//! comparison testing. +//! them to the MA/AQ tag format (lengths are inline in the MA string). //! //! Run with: //! cargo run --example fiberseq_to_ma diff --git a/molecular-annotation/src/lib.rs b/molecular-annotation/src/lib.rs index d146faa6..f81d0450 100644 --- a/molecular-annotation/src/lib.rs +++ b/molecular-annotation/src/lib.rs @@ -1,7 +1,7 @@ //! Molecular Annotation Library //! //! This library provides types and functions for working with molecular annotations -//! according to the MA/AL/AQ/AN tag specification for SAM/BAM files. +//! according to the MA/AQ/AN tag specification for SAM/BAM files. //! //! # Coordinate Conventions //! From 406ddccf216002157335e0ac1712dc0afad3e39e Mon Sep 17 00:00:00 2001 From: "Mitchell R. Vollger" Date: Thu, 13 Aug 2026 10:30:48 -0700 Subject: [PATCH 5/5] refactor: split legacy-tag stripping out to its own PR (#124) Keeps this PR scoped to the FIRE-qual overlay fixes (qc, decorators, filter expressions, mock-fire). The write_record legacy-tag hygiene and its tests move to fix/legacy-tag-hygiene, where they ship together with the Ma/Aq/An read-tolerance work. Co-Authored-By: Claude Fable 5 --- src/subcommands/convert_tags.rs | 21 ++++-- src/utils/ma_io.rs | 123 +++---------------------------- tests/regression/convert_tags.rs | 48 ------------ tests/regression/fire.rs | 26 ------- 4 files changed, 24 insertions(+), 194 deletions(-) diff --git a/src/subcommands/convert_tags.rs b/src/subcommands/convert_tags.rs index 76060d79..89f7889c 100644 --- a/src/subcommands/convert_tags.rs +++ b/src/subcommands/convert_tags.rs @@ -12,14 +12,11 @@ use rust_htslib::bam::Read; /// dropped on the way in (the legacy `aq` byte becomes the separate `fire` /// type), so converted MSP tags carry no qualities. /// -/// Legacy tags (including the fibertig `fs`/`fl`/`fa` set) are removed -/// **only** when they were the source of the annotations, i.e. the record had -/// no `MA` tag — this provenance rule is enforced by `ma_io::write_record` -/// itself, shared with every other MA-writing subcommand. A record that -/// already carries an `MA` tag keeps its legacy-named aux tags untouched, so -/// we never strip identically-named tags written by another tool. `MM`/`ML` -/// are preserved byte-identically (this path does not touch base -/// modifications). +/// Legacy tags are removed **only** when they were the source of the +/// annotations, i.e. the record had no `MA` tag. A record that already carries +/// an `MA` tag is passed through untouched, so we never strip identically-named +/// aux tags written by another tool. `MM`/`ML` are preserved byte-identically +/// (this path does not touch base modifications). pub fn convert_tags(opts: &mut cli::ConvertTagsOptions) { let mut bam = opts.input.bam_reader(); let mut out = opts.input.bam_writer(&opts.out); @@ -27,9 +24,17 @@ pub fn convert_tags(opts: &mut cli::ConvertTagsOptions) { for rec in bam.records() { let mut record = rec.expect("failed to read BAM record"); + let had_ma = record.aux(b"MA").is_ok(); let annot = ma_io::read_record(&record) .unwrap_or_else(|e| panic!("failed to read annotations: {e}")); + // Provenance guard: only remove legacy tags we actually consumed. + if !had_ma { + for tag in ma_io::LEGACY_READ_TAGS { + record.remove_aux(tag).ok(); + } + } + ma_io::write_record(&mut record, &annot); out.write(&record).expect("failed to write BAM record"); bar.inc(1); diff --git a/src/utils/ma_io.rs b/src/utils/ma_io.rs index 0e3022d2..1927e32b 100644 --- a/src/utils/ma_io.rs +++ b/src/utils/ma_io.rs @@ -34,11 +34,6 @@ pub const FIRE_TYPE: &str = "fire"; /// MA tag is present. These are never emitted; we only ingest them. pub const LEGACY_READ_TAGS: &[&[u8]] = &[b"ns", b"nl", b"as", b"al", b"aq"]; -/// Legacy fibertig tags, likewise consumed only as a read fallback (see -/// [`read_legacy_fibertig`]) and stripped alongside [`LEGACY_READ_TAGS`] -/// when they were the source of a written annotation model. -pub const LEGACY_FIBERTIG_TAGS: &[&[u8]] = &[b"fs", b"fl", b"fa"]; - /// Molecular-orientation nuc/msp arrays returned by [`extract_nuc_msp_arrays`]: /// `(nuc_starts, nuc_lengths, msp_starts, msp_lengths, msp_qual)`. `msp_qual` /// is empty when there are no MSP qualities (pre-FIRE state). @@ -64,19 +59,20 @@ pub fn read_record(record: &bam::Record) -> Result { // legacy fallback whenever MM/ML is present). let has_ma = matches!(record.aux(b"MA"), Ok(Aux::String(_))); if !has_ma { - // Provenance: the gates are type-checked (`has_legacy_nuc_msp`, - // `has_legacy_fibertig`), so a foreign tool reusing these two-letter - // names with a different aux type is never parsed as fibertools data. - // The write path (`strip_consumed_legacy_tags`) removes a legacy tag - // only under these same gates plus a per-tag type check — i.e. only - // when this reader consumed it — so keep the two in sync. - if has_legacy_nuc_msp(record) { + // Provenance caveat: presence of `ns`/`as`/`fs` is treated as evidence + // of fibertools-legacy data with no further validation. This path is + // read-only — we never delete these tags — so a foreign tool reusing + // those names is at worst mis-parsed here, never destroyed. Any future + // code that *removes* legacy tags must verify provenance first. + let has_legacy_nuc = record.aux(b"ns").is_ok(); + let has_legacy_msp = record.aux(b"as").is_ok(); + if has_legacy_nuc || has_legacy_msp { merge_missing_types(&mut annot, read_legacy_nuc_msp(record)?); } // Legacy fibertig (`fs`/`fl`/`fa`): the pre-MA fibertig wire format, // dropped from the writer in favour of the MA-spec `AN` tag. Kept - // readable so older fibertig BAMs stay consumable by `extract`. - if has_legacy_fibertig(record) { + // read-only so older fibertig BAMs stay consumable by `extract`. + if record.aux(b"fs").is_ok() { merge_missing_types(&mut annot, read_legacy_fibertig(record)?); } } @@ -137,68 +133,9 @@ fn merge_missing_types(dst: &mut MolecularAnnotations, src: MolecularAnnotations /// Producers that create or modify base mods must instead call /// [`write_record_with_basemods`], which canonically re-emits MM/ML. pub fn write_record(record: &mut bam::Record, annot: &MolecularAnnotations) { - strip_consumed_legacy_tags(record); annot.to_record(record); } -/// Provenance rule shared by every MA write: strip exactly the legacy tag -/// sets that [`read_record`] consumed as the source of the annotation model -/// being written — they are superseded by the MA tags (v0.9 replace -/// semantics; otherwise legacy readers silently see stale calls forever). -/// -/// The gates mirror [`read_record`]'s consumption gates, which is what makes -/// this provenance-safe: a set is only removed when the reader ingested it -/// (and it parsed as fibertools data — a parse failure errors out before any -/// write). Records that already carry `MA` were not read from legacy tags, -/// so their legacy-named aux tags are left untouched: a foreign tool reusing -/// those names is never destroyed. Likewise a lone foreign tag outside its -/// set's gate (e.g. `nl` with no `ns`/`as`) is left alone. -fn strip_consumed_legacy_tags(record: &mut bam::Record) { - if matches!(record.aux(b"MA"), Ok(Aux::String(_))) { - return; - } - if has_legacy_nuc_msp(record) { - for tag in LEGACY_READ_TAGS { - if is_legacy_typed(record, tag) { - record.remove_aux(tag).ok(); - } - } - } - if has_legacy_fibertig(record) { - for tag in LEGACY_FIBERTIG_TAGS { - if is_legacy_typed(record, tag) { - record.remove_aux(tag).ok(); - } - } - } -} - -/// Type-checked consumption gate for the legacy nuc/msp tag set. Presence -/// alone is weak provenance — a foreign tool could reuse these two-letter -/// names with a different aux type — so the set only counts as -/// fibertools-legacy when a gate tag parses as the int array legacy -/// fibertools wrote. -fn has_legacy_nuc_msp(record: &bam::Record) -> bool { - u32_array(record, b"ns").is_some() || u32_array(record, b"as").is_some() -} - -/// Type-checked consumption gate for the legacy fibertig tag set. -fn has_legacy_fibertig(record: &bam::Record) -> bool { - u32_array(record, b"fs").is_some() && u32_array(record, b"fl").is_some() -} - -/// True when `tag` carries the aux type legacy fibertools wrote for it — -/// the per-tag half of the provenance check in -/// [`strip_consumed_legacy_tags`]: even inside a gated set, a wrong-typed -/// tag was not consumed by the reader and so is never removed. -fn is_legacy_typed(record: &bam::Record, tag: &[u8]) -> bool { - match tag { - b"aq" => u8_array(record, tag).is_some(), - b"fa" => matches!(record.aux(tag), Ok(Aux::String(_))), - _ => u32_array(record, tag).is_some(), - } -} - /// Writes MA-family tags **and** canonically re-encodes MM/ML from the /// annotation model. /// @@ -214,7 +151,7 @@ fn is_legacy_typed(record: &bam::Record, tag: &[u8]) -> bool { /// Basemod types must already be `Encoding::MmMl` — they are when read from a /// record, and producers create them that way (e.g. `Encoding::mm_ml()`). pub fn write_record_with_basemods(record: &mut bam::Record, annot: &MolecularAnnotations) { - write_record(record, annot); + annot.to_record(record); annot.write_mm_ml(record); } @@ -558,44 +495,6 @@ mod tests { use super::*; use molecular_annotation::{MolecularAnnotations, QualitySpec, Strand}; - /// Foreign tags that merely reuse legacy names with a different aux type - /// must never be stripped by the MA write path; correctly-typed consumed - /// legacy tags must be. - #[test] - fn strip_consumed_legacy_tags_checks_types() { - // foreign: `ns` as a string, `al` as a float — not fibertools-typed - let mut foreign = synth_record(b"ACGTACGT"); - foreign.push_aux(b"ns", Aux::String("not-ours")).unwrap(); - foreign.push_aux(b"al", Aux::Float(1.5)).unwrap(); - strip_consumed_legacy_tags(&mut foreign); - assert!(foreign.aux(b"ns").is_ok(), "foreign string ns was stripped"); - assert!(foreign.aux(b"al").is_ok(), "foreign float al was stripped"); - - // fibertools-typed legacy nuc/msp set: consumed, so stripped — but a - // wrong-typed member of the set (string aq) survives - let mut legacy = synth_record(b"ACGTACGT"); - legacy - .push_aux(b"ns", Aux::ArrayU32((&vec![1u32, 5]).into())) - .unwrap(); - legacy - .push_aux(b"nl", Aux::ArrayU32((&vec![2u32, 2]).into())) - .unwrap(); - legacy.push_aux(b"aq", Aux::String("not-ours")).unwrap(); - strip_consumed_legacy_tags(&mut legacy); - assert!(legacy.aux(b"ns").is_err(), "consumed ns not stripped"); - assert!(legacy.aux(b"nl").is_err(), "consumed nl not stripped"); - assert!(legacy.aux(b"aq").is_ok(), "wrong-typed aq was stripped"); - - // a record already carrying MA keeps even fibertools-typed legacy tags - let mut with_ma = synth_record(b"ACGTACGT"); - with_ma.push_aux(b"MA", Aux::String("8;")).unwrap(); - with_ma - .push_aux(b"ns", Aux::ArrayU32((&vec![1u32]).into())) - .unwrap(); - strip_consumed_legacy_tags(&mut with_ma); - assert!(with_ma.aux(b"ns").is_ok(), "legacy tag stripped despite MA"); - } - /// Build a synthetic record with the given forward sequence. Aligned-blocks /// metadata is not needed for these I/O tests because we only exercise /// tag-level round-trips, not coordinate liftovers. diff --git a/tests/regression/convert_tags.rs b/tests/regression/convert_tags.rs index bdeb16cb..5aa46b16 100644 --- a/tests/regression/convert_tags.rs +++ b/tests/regression/convert_tags.rs @@ -96,51 +96,3 @@ fn convert_tags_is_idempotent() { } } } - -// Legacy fibertig fs/fl/fa tags are consumed by the reader (into the MA -// fibertig type) and must be stripped on conversion just like ns/nl/as/al/aq -// — otherwise the source tags survive as duplicate, potentially-stale copies. -#[test] -fn convert_tags_strips_consumed_fibertig_tags() { - use rust_htslib::bam::Header; - - // synthesize a legacy fibertig BAM from the first msp_nuc.bam record - let input = fixture("msp_nuc.bam"); - let mut reader = bam::Reader::from_path(&input).unwrap(); - let header = Header::from_template(reader.header()); - let synth = NamedTempFile::with_suffix(".bam").unwrap(); - { - let mut writer = bam::Writer::from_path(synth.path(), &header, bam::Format::Bam).unwrap(); - let mut rec = reader.records().next().unwrap().unwrap(); - rec.push_aux(b"fs", Aux::ArrayU32((&vec![100u32, 500]).into())) - .unwrap(); - rec.push_aux(b"fl", Aux::ArrayU32((&vec![50u32, 60]).into())) - .unwrap(); - rec.push_aux(b"fa", Aux::String("gene_a|")).unwrap(); - writer.write(&rec).unwrap(); - } - - let out = NamedTempFile::with_suffix(".bam").unwrap(); - convert(synth.path(), out.path()); - let rec = &records(out.path())[0]; - for tag in [b"fs", b"fl", b"fa"] { - assert!( - rec.aux(tag).is_err(), - "fibertig tag {} survived conversion", - String::from_utf8_lossy(tag) - ); - } - let ma_tag = ma(rec).expect("MA tag not written"); - assert!( - ma_tag.contains("fibertig"), - "fibertig annotations not carried into MA tag: {ma_tag}" - ); - let an = match rec.aux(b"AN") { - Ok(Aux::String(s)) => s.to_string(), - _ => String::new(), - }; - assert!( - an.contains("gene_a"), - "fibertig name not carried into AN tag: {an}" - ); -} diff --git a/tests/regression/fire.rs b/tests/regression/fire.rs index 631394ef..e269f17d 100644 --- a/tests/regression/fire.rs +++ b/tests/regression/fire.rs @@ -1,5 +1,4 @@ use super::common::{fixture, run, select_tsv_cols}; -use rust_htslib::bam::{self, Read}; use tempfile::NamedTempFile; fn extract_fdrs(out: &str) -> Vec { @@ -48,31 +47,6 @@ fn filter_expression_qual_msp_uses_fire_quals() { ); } -// `ft fire` on a legacy-tag BAM consumes ns/nl/as/al/aq and writes MA tags; -// the consumed legacy tags must be stripped (v0.9 replace semantics) or -// legacy readers silently see stale calls forever. -#[test] -fn fire_on_legacy_input_strips_consumed_legacy_tags() { - let scored = NamedTempFile::with_suffix(".bam").unwrap(); - run(&[ - "fire", - fixture("msp_nuc.bam").to_str().unwrap(), - scored.path().to_str().unwrap(), - ]); - let mut reader = bam::Reader::from_path(scored.path()).unwrap(); - for rec in reader.records() { - let rec = rec.unwrap(); - assert!(rec.aux(b"MA").is_ok(), "record missing MA tag"); - for tag in [b"ns", b"nl", b"as", b"al", b"aq"] { - assert!( - rec.aux(tag).is_err(), - "stale legacy tag {} left on fire output", - String::from_utf8_lossy(tag) - ); - } - } -} - // `ft fire` stores FIRE calls on the `fire` annotation type (MA spec); the // MSPs themselves carry no quals. `--extract` must overlay the fire quals // onto the matching MSPs, otherwise every row comes out with FDR = 1.0 and