Error on scglue.genomics.rna_anchored_guidance_graph

[peiruzhang]

Hello, I have the error when running guidance = scglue.genomics.rna_anchored_guidance_graph(rna, atac) when using the data provided in the tutorial. The error message is as follows:

ValueError Traceback (most recent call last)
Cell In[58], line 2
1 #print(rna.var)
----> 2 guidance = scglue.genomics.rna_anchored_guidance_graph(rna, atac)
3 #print(len(atac))
4 #guidance

File ~/anaconda3/envs/glue/lib/python3.10/site-packages/scglue/genomics.py:528, in rna_anchored_guidance_graph(rna, gene_region, promoter_len, extend_range, extend_fn, signs, propagate_highly_variable, corrupt_rate, random_state, *others)
526 raise RuntimeError("Length of others and signs must match!")
527 if set(signs).difference({-1, 1}):
--> 528 raise RuntimeError("signs can only contain {-1, 1}!")
530 rna_bed = Bed(rna.var.assign(name=rna.var_names))
531 other_beds = [Bed(other.var.assign(name=other.var_names)) for other in others]

File ~/anaconda3/envs/glue/lib/python3.10/site-packages/scglue/utils.py:496, in ConstrainedDataFrame.init(self, *args, **kwargs)
494 df = pd.DataFrame(*args, **kwargs)
495 df = self.rectify(df)
--> 496 self.verify(df)
497 super().init(df)

File ~/anaconda3/envs/glue/lib/python3.10/site-packages/scglue/genomics.py:65, in Bed.verify(cls, df)
63 super(Bed, cls).verify(df)
64 print("当前列名：", df.columns)
---> 65 print("数据内容示例：", df.head().to_csv(sep='\t', na_rep='nan'))
66 if len(df.columns) != len(cls.COLUMNS) or np.any(df.columns != cls.COLUMNS):
67 raise ValueError("Invalid BED format!")

ValueError: Invalid BED format!

When I deleted the duplicate columns in rna.var, it showed an error indicating that highly_variable was missing. Then I tried to remove the column name consistency check, and the following error message appeared.

I will appreciate if you could help me to figure it out. Thanks!