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cff-version: 1.2.0
message: "If you use MetaManifold in academic work, please cite it as follows."
title: "MetaManifold: a Julia orchestrator for amplicon sequencing pipelines with an interactive web interface"
type: software
authors:
- given-names: Joshua Benjamin
family-names: Jewell
email: jjewell23@rvc.ac.uk
affiliation: "Royal Veterinary College"
orcid: "https://orcid.org/0009-0007-0289-9741"
version: "0.1.0"
date-released: "2026-05-21"
license: AGPL-3.0-only
repository-code: "https://github.com/JoshuaJewell/MetaManifold-WebUI"
url: "https://github.com/JoshuaJewell/MetaManifold-WebUI"
abstract: >-
MetaManifold is a Julia-native orchestrator for amplicon sequencing
workflows. It wraps cutadapt, DADA2, SWARM, vsearch, and cd-hit-est into a
single configurable pipeline, storing per-run results in DuckDB and exposing
them through an interactive React frontend. Analyses include alpha diversity,
taxonomic composition, NMDS, and PERMANOVA via R/vegan, together with
cross-run comparison and a functional-database annotation layer.
keywords:
- amplicon sequencing
- metabarcoding
- DADA2
- SWARM
- bioinformatics
- Julia
- DuckDB
references:
- type: article
title: "DADA2: High-resolution sample inference from Illumina amplicon data"
authors:
- family-names: Callahan
given-names: "Benjamin J."
- family-names: McMurdie
given-names: "Paul J."
- family-names: Rosen
given-names: "Michael J."
- family-names: Han
given-names: "Andrew W."
- family-names: Johnson
given-names: "Amy Jo A."
- family-names: Holmes
given-names: "Susan P."
journal: "Nature Methods"
year: 2016
volume: 13
issue: 7
start: 581
end: 583
doi: "10.1038/nmeth.3869"
- type: article
title: "Swarm v2: highly-scalable and high-resolution amplicon clustering"
authors:
- family-names: Mahe
given-names: "Frederic"
- family-names: Rognes
given-names: "Torbjorn"
- family-names: Quince
given-names: "Christopher"
- family-names: "de Vargas"
given-names: "Colomban"
- family-names: Dunthorn
given-names: "Micah"
journal: "PeerJ"
year: 2015
volume: 3
start: e1420
doi: "10.7717/peerj.1420"
- type: article
title: "VSEARCH: a versatile open source tool for metagenomics"
authors:
- family-names: Rognes
given-names: "Torbjorn"
- family-names: Flouri
given-names: "Tomas"
- family-names: Nichols
given-names: "Ben"
- family-names: Quince
given-names: "Christopher"
- family-names: Mahe
given-names: "Frederic"
journal: "PeerJ"
year: 2016
volume: 4
start: e2584
doi: "10.7717/peerj.2584"