To whom it may concerned.
Hi, I am currently testing stormseq with my samples.
However, I got stuck on the page of stormseq with serveral errors.
The error comment is like below.
Case 1. There was an error : file-fail not found.
Case 2. There was an error : qc-fail Malformed paired end file. (read name should have /1 or /2 tag)
Let me tell you about my env. First.
I am trying to test my 2 files(3.7GB each) which are one sample’s paired end seq. result.
The data type I tried is illumina truseq and File extension is XXX_R1.fastq.gz / XXX_R2.fastq.gz. (except that, every options are as default (BWA, GATK Lite, etc)
So technically, the number of genome is one (2 files though). So I checked [One] in test page and got the error case 1 above. (Of course the sample is in root position in my bucket)
Then tried [Multiple] with files in same folder as the page guided but got the error case 2. (as far as I know, My sample have tags in the head)
In this stage, I cannot proceed the test any more so I need your help.
Here is my question below.
- I need to know the exact meaning of the option [One / Multiple] and What I should checked in my case (again, one samples paired end seq. 2 files as result)
- Is there any additional format or requirements for the .fastq file? If so, Please let me know about it.
Looking forward to having your answer and thank you in advance.
Thanks & Regards,
Jaehoon, Jang
To whom it may concerned.
Hi, I am currently testing stormseq with my samples.
However, I got stuck on the page of stormseq with serveral errors.
The error comment is like below.
Case 1. There was an error : file-fail not found.
Case 2. There was an error : qc-fail Malformed paired end file. (read name should have /1 or /2 tag)
Let me tell you about my env. First.
I am trying to test my 2 files(3.7GB each) which are one sample’s paired end seq. result.
The data type I tried is illumina truseq and File extension is XXX_R1.fastq.gz / XXX_R2.fastq.gz. (except that, every options are as default (BWA, GATK Lite, etc)
So technically, the number of genome is one (2 files though). So I checked [One] in test page and got the error case 1 above. (Of course the sample is in root position in my bucket)
Then tried [Multiple] with files in same folder as the page guided but got the error case 2. (as far as I know, My sample have tags in the head)
In this stage, I cannot proceed the test any more so I need your help.
Here is my question below.
Looking forward to having your answer and thank you in advance.
Thanks & Regards,
Jaehoon, Jang