diff --git a/.chronus/changes/fix-query-matcher-handling-2026-4-3.md b/.chronus/changes/fix-query-matcher-handling-2026-4-3.md
new file mode 100644
index 00000000000..c0a07d92c41
--- /dev/null
+++ b/.chronus/changes/fix-query-matcher-handling-2026-4-3.md
@@ -0,0 +1,7 @@
+---
+changeKind: fix
+packages:
+  - "@typespec/spector"
+---
+
+Fix query parameter matcher handling: use `resolveMatchers: false` so matcher objects (e.g. `match.dateTime`) are checked semantically instead of being serialized to plain strings before comparison.
diff --git a/packages/spec-api/test/match-engine.test.ts b/packages/spec-api/test/match-engine.test.ts
index c1ec6f01ea9..689f642b4e4 100644
--- a/packages/spec-api/test/match-engine.test.ts
+++ b/packages/spec-api/test/match-engine.test.ts
@@ -217,6 +217,62 @@ describe("integration with expandDyns", () => {
   });
 });
 
+describe("integration with expandDyns({ resolveMatchers: false })", () => {
+  const config: ResolverConfig = { baseUrl: "http://localhost:3000" };
+
+  it("should preserve matcher objects instead of resolving them to plain strings", () => {
+    const content = { timestamp: match.dateTime.rfc3339("2022-08-26T18:38:00.000Z") };
+    const expanded = expandDyns(content, config, { resolveMatchers: false });
+    // Matcher must survive as a matcher, not be converted to a plain string
+    expect(isMatcher(expanded.timestamp)).toBe(true);
+  });
+
+  it("should allow matchValues to do semantic datetime comparison after expandDyns with resolveMatchers:false", () => {
+    // Regression test: query params/headers with datetime matchers must use semantic comparison.
+    // Without resolveMatchers:false, expandDyns converts the matcher to the plain string
+    // "2022-08-26T18:38:00.000Z", and a strict === comparison against the actual value
+    // "2022-08-26T18:38:00Z" (no milliseconds) would fail even though they represent the
+    // same point in time.
+    const queryDef = { input: match.dateTime.utcRfc3339("2022-08-26T18:38:00.000Z") };
+    const expanded = expandDyns(queryDef, config, { resolveMatchers: false });
+
+    // The actual query string received from an HTTP request (no milliseconds)
+    const actualQueryValue = "2022-08-26T18:38:00Z";
+
+    // Simulates what createHandler does: isMatcher → deepEqual → matchValues → matcher.check()
+    expect(isMatcher(expanded.input)).toBe(true);
+    expectPass(matchValues(actualQueryValue, expanded.input, "$", config));
+  });
+
+  it("should allow matchValues to do semantic datetime comparison for header values after expandDyns with resolveMatchers:false", () => {
+    // Regression test: headers with datetime matchers must use semantic comparison, same as query params.
+    // Without resolveMatchers:false the matcher is serialized early and isMatcher() returns false,
+    // so the code falls through to containsHeader() with String(value) — a strict string equality
+    // that fails for semantically equivalent but format-different datetime strings.
+    const headerDef = { "x-ms-date": match.dateTime.rfc7231("Fri, 26 Aug 2022 18:38:00 GMT") };
+    const expanded = expandDyns(headerDef, config, { resolveMatchers: false });
+
+    // isMatcher must still be true so createHandler routes through deepEqual / matchValues
+    expect(isMatcher(expanded["x-ms-date"])).toBe(true);
+    // Semantic check passes for the exact same RFC 7231 string
+    expectPass(matchValues("Fri, 26 Aug 2022 18:38:00 GMT", expanded["x-ms-date"], "$", config));
+  });
+
+  it("should demonstrate why resolveMatchers:true (default) breaks semantic query param matching", () => {
+    // With the default resolveMatchers:true, the matcher is eagerly converted to a plain string.
+    // A strict string comparison then fails for semantically equivalent but format-different values.
+    const queryDef = { input: match.dateTime.utcRfc3339("2022-08-26T18:38:00.000Z") };
+    const expandedWithResolve = expandDyns(queryDef, config); // resolveMatchers: true (default)
+
+    // The matcher is gone — replaced by its serialized string
+    expect(isMatcher(expandedWithResolve.input)).toBe(false);
+    expect(expandedWithResolve.input).toBe("2022-08-26T18:38:00.000Z");
+
+    // Strict string comparison fails for an equivalent datetime without milliseconds
+    expect(expandedWithResolve.input === "2022-08-26T18:38:00Z").toBe(false);
+  });
+});
+
 describe("integration with json() Resolver", () => {
   const config: ResolverConfig = { baseUrl: "http://localhost:3000" };
 
diff --git a/packages/spector/src/app/app.ts b/packages/spector/src/app/app.ts
index c2f6a8ef311..a6b5cc2c62a 100644
--- a/packages/spector/src/app/app.ts
+++ b/packages/spector/src/app/app.ts
@@ -1,5 +1,6 @@
 import {
   expandDyns,
+  isMatcher,
   MockApiDefinition,
   MockBody,
   MockMultipartBody,
@@ -145,10 +146,10 @@ function createHandler(apiDefinition: MockApiDefinition, config: ResolverConfig)
 
     // Validate headers if present in the request
     if (apiDefinition.request?.headers) {
-      const headers = expandDyns(apiDefinition.request.headers, config);
+      const headers = expandDyns(apiDefinition.request.headers, config, { resolveMatchers: false });
       Object.entries(headers).forEach(([key, value]) => {
         if (key.toLowerCase() !== "content-type") {
-          if (Array.isArray(value)) {
+          if (isMatcher(value) || Array.isArray(value)) {
             req.expect.deepEqual(req.headers[key], value);
           } else {
             req.expect.containsHeader(key.toLowerCase(), String(value));
@@ -158,9 +159,9 @@ function createHandler(apiDefinition: MockApiDefinition, config: ResolverConfig)
     }
 
     if (apiDefinition.request?.query) {
-      const query = expandDyns(apiDefinition.request.query, config);
+      const query = expandDyns(apiDefinition.request.query, config, { resolveMatchers: false });
       Object.entries(query).forEach(([key, value]) => {
-        if (Array.isArray(value)) {
+        if (isMatcher(value) || Array.isArray(value)) {
           req.expect.deepEqual(req.query[key], value);
         } else {
           req.expect.containsQueryParam(key, String(value));