diff --git a/browser/app/profile/firefox.js b/browser/app/profile/firefox.js
index 4dd626ad22ab3..8d173b156a406 100644
--- a/browser/app/profile/firefox.js
+++ b/browser/app/profile/firefox.js
@@ -2874,7 +2874,12 @@ pref("browser.migrate.preferences-entrypoint.enabled", true);
 // "offered"        - we have offered feature to user and they have not yet made a decision.
 // "enabled"        - user opted in to the feature.
 // "disabled"       - user opted out of the feature.
+#ifdef MOZ_ENTERPRISE
+// Firefox Relay is not supported in enterprise builds
+pref("signon.firefoxRelay.feature", "unavailable");
+#else
 pref("signon.firefoxRelay.feature", "available");
+#endif
 pref("signon.management.page.breach-alerts.enabled", true);
 pref("signon.management.page.vulnerable-passwords.enabled", true);
 pref("signon.management.page.sort", "name");
diff --git a/toolkit/components/passwordmgr/test/browser/browser.toml b/toolkit/components/passwordmgr/test/browser/browser.toml
index 11e090b83c49c..a4329460e7371 100644
--- a/toolkit/components/passwordmgr/test/browser/browser.toml
+++ b/toolkit/components/passwordmgr/test/browser/browser.toml
@@ -270,17 +270,25 @@ skip-if = [
   "os == 'win' && os_version == '11.26200' && verify-standalone",
 ]
 
+["browser_relay_disabled_enterprise.js"]
+support-files = ["browser_relay_utils.js"]
+run-if = ["enterprise"]
+
 ["browser_relay_signup_flow.js"]
 support-files = ["browser_relay_utils.js"]
+skip-if = ["enterprise"]
 
 ["browser_relay_signup_flow_showToAllBrowsers.js"]
 support-files = ["browser_relay_utils.js"]
+skip-if = ["enterprise"]
 
 ["browser_relay_telemetry.js"]
 support-files = ["browser_relay_utils.js"]
+skip-if = ["enterprise"]
 
 ["browser_relay_use.js"]
 support-files = ["browser_relay_utils.js"]
+skip-if = ["enterprise"]
 
 ["browser_rust_mirror.js"]
 skip-if = [
diff --git a/toolkit/components/passwordmgr/test/browser/browser_relay_disabled_enterprise.js b/toolkit/components/passwordmgr/test/browser/browser_relay_disabled_enterprise.js
new file mode 100644
index 0000000000000..4ad60dc1cf8c1
--- /dev/null
+++ b/toolkit/components/passwordmgr/test/browser/browser_relay_disabled_enterprise.js
@@ -0,0 +1,50 @@
+const TEST_URL_PATH = `https://example.org${DIRECTORY_PATH}form_basic_signup.html`;
+
+Services.scriptloader.loadSubScript(
+  "chrome://mochitests/content/browser/toolkit/components/passwordmgr/test/browser/browser_relay_utils.js",
+  this
+);
+
+add_task(async function test_enterprise_relay_feature_default_disabled() {
+  Assert.equal(
+    Services.prefs
+      .getDefaultBranch("")
+      .getStringPref("signon.firefoxRelay.feature"),
+    "unavailable",
+    "signon.firefoxRelay.feature default should be 'unavailable' in enterprise builds"
+  );
+});
+
+add_task(async function test_enterprise_relay_utils_reports_disabled() {
+  const { FirefoxRelayUtils } = ChromeUtils.importESModule(
+    "resource://gre/modules/FirefoxRelayUtils.sys.mjs"
+  );
+
+  Assert.ok(
+    !FirefoxRelayUtils.relayIsAvailableOrEnabled(),
+    "FirefoxRelayUtils.relayIsAvailableOrEnabled() should return false when feature is disabled"
+  );
+});
+
+add_task(
+  async function test_enterprise_relay_autocomplete_not_shown_when_disabled() {
+    const rsSandbox = await stubRemoteSettingsAllowList();
+    await BrowserTestUtils.withNewTab(
+      {
+        gBrowser,
+        url: TEST_URL_PATH,
+      },
+      async function (browser) {
+        const popup = document.getElementById("PopupAutoComplete");
+        await openACPopup(popup, browser, "#form-basic-username");
+
+        const relayItem = getRelayItemFromACPopup(popup);
+        Assert.ok(
+          !relayItem,
+          "Relay item SHOULD NOT be present in the autocomplete popup when the feature is disabled"
+        );
+      }
+    );
+    rsSandbox.restore();
+  }
+);