Context
Document rationale for using "Josiah" as reference
Josiah has a longer reference sequence compared to Pinneo
- Commonly used as a comparison standard in laboratory settings
- Designated as the REFSEQ for Lassa virus
I got confused since the tree was rooted to "Pinneo", which highlights to me the need to clarify the distinctions between:
- Reference sequence: Used for alignment and translation, affecting codon numbering and indel comparisons
- Root sequence: Provides consistency in phylogenetic tree rooting
- Reconstructed root: May be necessary for consistent tree rooting when dealing with numerous recent diverse samples but limited historic samples for resolving the tree's trunk
For more information: https://docs.nextstrain.org/en/latest/guides/bioinformatics/root-and-ref-seqs.html
Related to historic issue which set reference to Josiah:
Description
Examples
Possible solution
Context
Document rationale for using "Josiah" as reference
Josiahhas a longer reference sequence compared toPinneoI got confused since the tree was rooted to "Pinneo", which highlights to me the need to clarify the distinctions between:
For more information: https://docs.nextstrain.org/en/latest/guides/bioinformatics/root-and-ref-seqs.html
Related to historic issue which set reference to
Josiah:Description
Examples
Possible solution