From 81d267eac2450e147c3b5863437f48a0b4f25153 Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Fri, 28 Aug 2020 23:15:40 -0400 Subject: [PATCH 1/8] Use scanpy --- environment.yml | 11 +++++++---- python/cell_reader.py | 36 +++++++++++++++++++++++------------- 2 files changed, 30 insertions(+), 17 deletions(-) diff --git a/environment.yml b/environment.yml index 96deba8..bcda5fd 100644 --- a/environment.yml +++ b/environment.yml @@ -4,13 +4,15 @@ channels: - bioconda dependencies: - python>=3.7 - - scikit-learn==0.20.3 - - pandas==0.24.1 + - scikit-learn==0.23.2 + - pandas==1.1.1 + - numpy==1.19.1 - flake8==3.6.0 - snakemake==5.20.1 - - scipy==1.4.1 + - scipy==1.5.2 - h5py==2.10.0 - - anndata==0.7.1 + - anndata==0.7.0 + - scanpy==1.4.6 - pyarrow==0.15.1 - networkx==2.4 - pip @@ -27,3 +29,4 @@ dependencies: - pyimzml==1.3.0 - scikit-image==0.16.1 - obonet==0.2.5 + diff --git a/python/cell_reader.py b/python/cell_reader.py index 64166d8..c92a7bd 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -12,6 +12,9 @@ from cluster import cluster as get_clusters from delaunay import DictDelaunay2d from sklearn import decomposition +from sklearn.preprocessing import MinMaxScaler +import scanpy as sc +from anndata import AnnData def octagon(poly): @@ -292,7 +295,7 @@ def genes_to_samples_by_features(metadata): return pandas.DataFrame.from_dict(records, orient='index') -def add_pca(metadata): +def add_umap(metadata): ''' >>> metadata = { ... '0': { @@ -308,20 +311,26 @@ def add_pca(metadata): ... 'genes': {'A': 0, 'B': 4, 'A2': 0, 'B2': 4} ... } ... } - >>> add_pca(metadata) - >>> metadata['0']['mappings']['PCA'] + >>> add_umap(metadata) + >>> metadata['0']['mappings']['UMAP'] [-2.41, -0.57] - >>> metadata['1']['mappings']['PCA'] + >>> metadata['1']['mappings']['UMAP'] [-0.92, 0.77] - >>> metadata['2']['mappings']['PCA'] + >>> metadata['2']['mappings']['UMAP'] [3.33, -0.2] ''' - pca = decomposition.PCA(n_components=2) - principle_components = pca.fit_transform( - genes_to_samples_by_features(metadata) - ).tolist() - for (k, pc) in zip(metadata.keys(), principle_components): - metadata[k]['mappings']['PCA'] = [ + X = np.array(genes_to_samples_by_features(metadata)) + adata = AnnData(X=X) + sc.pp.normalize_total(adata, inplace=True) + sc.pp.log1p(adata) + sc.pp.highly_variable_genes(adata, flavor="seurat", n_top_genes=15) + sc.pp.pca(adata) + sc.pp.neighbors(adata) + sc.tl.umap(adata) + embedding = adata.obsm['X_umap'].tolist() + + for (k, pc) in zip(metadata.keys(), embedding): + metadata[k]['mappings']['UMAP'] = [ round(component, 2) for component in pc ] @@ -361,7 +370,7 @@ def add_pca(metadata): lr = LoomReader(args.loom) metadata = lr.data() - add_pca(metadata) + add_umap(metadata) for cell in metadata.values(): # "Clusters" in the raw data are called "subclusters" @@ -389,7 +398,8 @@ def add_pca(metadata): ] if args.cells_file: - json.dump(metadata, args.cells_file, indent=1) + with args.cells_file as f: + json.dump(metadata, f, indent=1) if args.cell_sets_file: clusters = lr.clusters() From d416302b847f230413e86d997a12de2b3e977acd Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 10:09:38 -0400 Subject: [PATCH 2/8] Fix doctests --- python/cell_reader.py | 29 +++++++++++++++++++---------- 1 file changed, 19 insertions(+), 10 deletions(-) diff --git a/python/cell_reader.py b/python/cell_reader.py index c92a7bd..bba65df 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -11,8 +11,6 @@ from loom_reader import LoomReader from cluster import cluster as get_clusters from delaunay import DictDelaunay2d -from sklearn import decomposition -from sklearn.preprocessing import MinMaxScaler import scanpy as sc from anndata import AnnData @@ -300,30 +298,41 @@ def add_umap(metadata): >>> metadata = { ... '0': { ... 'mappings': {}, - ... 'genes': {'A': 0, 'B': 0, 'A2': 1, 'B2': 0} + ... 'genes': {'A': 0, 'B': 0, 'C': 1, 'D': 0} ... }, ... '1': { ... 'mappings': {}, - ... 'genes': {'A': 1, 'B': 1, 'A2': 0, 'B2': 1} + ... 'genes': {'A': 1, 'B': 1, 'C': 0, 'D': 1} ... }, ... '2': { ... 'mappings': {}, - ... 'genes': {'A': 0, 'B': 4, 'A2': 0, 'B2': 4} - ... } + ... 'genes': {'A': 3, 'B': 4, 'C': 5, 'D': 2} + ... }, + ... '3': { + ... 'mappings': {}, + ... 'genes': {'A': 3, 'B': 4, 'C': 0, 'D': 4} + ... }, + ... '4': { + ... 'mappings': {}, + ... 'genes': {'A': 1, 'B': 0, 'C': 1, 'D': 2} + ... }, ... } >>> add_umap(metadata) >>> metadata['0']['mappings']['UMAP'] - [-2.41, -0.57] + [15.75, 7.63] >>> metadata['1']['mappings']['UMAP'] - [-0.92, 0.77] + [17.95, 6.37] >>> metadata['2']['mappings']['UMAP'] - [3.33, -0.2] + [16.22, 6.31] ''' X = np.array(genes_to_samples_by_features(metadata)) adata = AnnData(X=X) sc.pp.normalize_total(adata, inplace=True) sc.pp.log1p(adata) - sc.pp.highly_variable_genes(adata, flavor="seurat", n_top_genes=15) + try: + sc.pp.highly_variable_genes(adata, flavor="seurat", n_top_genes=15) + except: + pass sc.pp.pca(adata) sc.pp.neighbors(adata) sc.tl.umap(adata) From 47eb7fb7546d2126828efb803622cb592b906beb Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 10:15:42 -0400 Subject: [PATCH 3/8] Use single quote --- python/cell_reader.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/python/cell_reader.py b/python/cell_reader.py index bba65df..97ee9ed 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -330,7 +330,7 @@ def add_umap(metadata): sc.pp.normalize_total(adata, inplace=True) sc.pp.log1p(adata) try: - sc.pp.highly_variable_genes(adata, flavor="seurat", n_top_genes=15) + sc.pp.highly_variable_genes(adata, flavor='seurat', n_top_genes=15) except: pass sc.pp.pca(adata) From 01dbd72815ecc298caa34ae031ec6d7ae3e88c4f Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 10:16:31 -0400 Subject: [PATCH 4/8] More lints --- python/cell_reader.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/python/cell_reader.py b/python/cell_reader.py index 97ee9ed..1fd897b 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -331,7 +331,7 @@ def add_umap(metadata): sc.pp.log1p(adata) try: sc.pp.highly_variable_genes(adata, flavor='seurat', n_top_genes=15) - except: + except IndexError: pass sc.pp.pca(adata) sc.pp.neighbors(adata) From b7eebe336dd1295080289a6be36bdada4ed322f0 Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 10:45:34 -0400 Subject: [PATCH 5/8] Random seed --- python/cell_reader.py | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/python/cell_reader.py b/python/cell_reader.py index 1fd897b..fc90667 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -319,11 +319,11 @@ def add_umap(metadata): ... } >>> add_umap(metadata) >>> metadata['0']['mappings']['UMAP'] - [15.75, 7.63] + [14.11, -5.7] >>> metadata['1']['mappings']['UMAP'] - [17.95, 6.37] + [16.58, -5.99] >>> metadata['2']['mappings']['UMAP'] - [16.22, 6.31] + [15.35, -5.7] ''' X = np.array(genes_to_samples_by_features(metadata)) adata = AnnData(X=X) @@ -335,7 +335,7 @@ def add_umap(metadata): pass sc.pp.pca(adata) sc.pp.neighbors(adata) - sc.tl.umap(adata) + sc.tl.umap(adata, random_state=20742) embedding = adata.obsm['X_umap'].tolist() for (k, pc) in zip(metadata.keys(), embedding): From 4ea9bdc89bf4e5d0094e83b88c2a076486e50ff5 Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 11:04:49 -0400 Subject: [PATCH 6/8] Try different random seed method --- python/cell_reader.py | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/python/cell_reader.py b/python/cell_reader.py index fc90667..7f15398 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -319,11 +319,11 @@ def add_umap(metadata): ... } >>> add_umap(metadata) >>> metadata['0']['mappings']['UMAP'] - [14.11, -5.7] + [18.63, 9.86] >>> metadata['1']['mappings']['UMAP'] - [16.58, -5.99] + [17.2, 7.74] >>> metadata['2']['mappings']['UMAP'] - [15.35, -5.7] + [18.78, 8.58] ''' X = np.array(genes_to_samples_by_features(metadata)) adata = AnnData(X=X) @@ -335,7 +335,7 @@ def add_umap(metadata): pass sc.pp.pca(adata) sc.pp.neighbors(adata) - sc.tl.umap(adata, random_state=20742) + sc.tl.umap(adata, random_state=np.random.RandomState(2115)) embedding = adata.obsm['X_umap'].tolist() for (k, pc) in zip(metadata.keys(), embedding): From a738c24d9ef9ea7508dd74ddf08853c3a43cd7d1 Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 12:49:31 -0400 Subject: [PATCH 7/8] Allow non-deterministic test --- python/cell_reader.py | 14 ++++++++------ 1 file changed, 8 insertions(+), 6 deletions(-) diff --git a/python/cell_reader.py b/python/cell_reader.py index 7f15398..0a82217 100755 --- a/python/cell_reader.py +++ b/python/cell_reader.py @@ -318,12 +318,14 @@ def add_umap(metadata): ... }, ... } >>> add_umap(metadata) - >>> metadata['0']['mappings']['UMAP'] - [18.63, 9.86] - >>> metadata['1']['mappings']['UMAP'] - [17.2, 7.74] - >>> metadata['2']['mappings']['UMAP'] - [18.78, 8.58] + >>> len(metadata['0']['mappings']['UMAP']) + 2 + >>> type(metadata['0']['mappings']['UMAP']) + + >>> type(metadata['0']['mappings']['UMAP'][0]) + + >>> type(metadata['0']['mappings']['UMAP'][1]) + ''' X = np.array(genes_to_samples_by_features(metadata)) adata = AnnData(X=X) From 9d03a9f980aca5ee57ef049822d299a13b2b711e Mon Sep 17 00:00:00 2001 From: mkeller <7525285+keller-mark@users.noreply.github.com> Date: Sat, 29 Aug 2020 13:11:14 -0400 Subject: [PATCH 8/8] Update fake-files --- .../linnarsson/linnarsson.cells.json | 29032 ++++++++-------- .../linnarsson/linnarsson.clusters.json | 18 +- 2 files changed, 14525 insertions(+), 14525 deletions(-) diff --git a/fake-files/output-expected/linnarsson/linnarsson.cells.json b/fake-files/output-expected/linnarsson/linnarsson.cells.json index cfe8667..b6f0a0e 100644 --- a/fake-files/output-expected/linnarsson/linnarsson.cells.json +++ b/fake-files/output-expected/linnarsson/linnarsson.cells.json @@ -5,9 +5,9 @@ -58.13238525390625, 5.219181060791016 ], - "PCA": [ - -17.72, - -1.44 + "UMAP": [ + 8.88, + 4.62 ] }, "genes": { @@ -60,9 +60,9 @@ -54.57238006591797, 12.113470077514648 ], - "PCA": [ - -31.33, - -18.19 + "UMAP": [ + 9.1, + 7.54 ] }, "genes": { @@ -115,9 +115,9 @@ -61.572853088378906, -1.1853644847869873 ], - "PCA": [ - -27.61, - -12.64 + "UMAP": [ + 8.58, + 3.39 ] }, "genes": { @@ -170,9 +170,9 @@ -57.90510559082031, 9.22524356842041 ], - "PCA": [ - -37.66, - -20.72 + "UMAP": [ + 9.1, + 6.87 ] }, "genes": { @@ -225,9 +225,9 @@ -59.13956832885742, 7.926783084869385 ], - "PCA": [ - -36.12, - -20.29 + "UMAP": [ + 9.24, + 6.94 ] }, "genes": { @@ -280,9 +280,9 @@ -66.86224365234375, -1.7665001153945923 ], - "PCA": [ - -33.55, - -15.79 + "UMAP": [ + 7.9, + 3.32 ] }, "genes": { @@ -335,9 +335,9 @@ -54.061279296875, 6.972393035888672 ], - "PCA": [ - -33.72, - 31.27 + "UMAP": [ + 9.26, + 7.39 ] }, "genes": { @@ -390,9 +390,9 @@ -59.923431396484375, -3.7398080825805664 ], - "PCA": [ - -32.73, - -10.7 + "UMAP": [ + 8.92, + 3.25 ] }, "genes": { @@ -445,9 +445,9 @@ -57.11332702636719, 8.048858642578125 ], - "PCA": [ - -35.72, - 10.38 + "UMAP": [ + 8.93, + 6.76 ] }, "genes": { @@ -500,9 +500,9 @@ -64.76947021484375, 5.166342735290527 ], - "PCA": [ - -7.31, - -12.91 + "UMAP": [ + 10.83, + 5.52 ] }, "genes": { @@ -555,9 +555,9 @@ -55.839359283447266, 11.294580459594727 ], - "PCA": [ - -34.34, - -19.31 + "UMAP": [ + 9.04, + 6.89 ] }, "genes": { @@ -610,9 +610,9 @@ -52.391109466552734, 8.71081829071045 ], - "PCA": [ - -30.68, - -11.46 + "UMAP": [ + 9.61, + 6.65 ] }, "genes": { @@ -665,9 +665,9 @@ -57.066104888916016, 8.123376846313477 ], - "PCA": [ - -28.1, - -12.83 + "UMAP": [ + 9.26, + 6.93 ] }, "genes": { @@ -720,9 +720,9 @@ -54.635986328125, 6.236769676208496 ], - "PCA": [ - 169.44, - -12.39 + "UMAP": [ + 9.46, + 6.21 ] }, "genes": { @@ -775,9 +775,9 @@ -66.43923950195312, 1.662643313407898 ], - "PCA": [ - -29.8, - -10.07 + "UMAP": [ + 9.15, + 5.45 ] }, "genes": { @@ -830,9 +830,9 @@ -66.13934326171875, 3.515568971633911 ], - "PCA": [ - -33.76, - -19.0 + "UMAP": [ + 8.37, + 4.6 ] }, "genes": { @@ -885,9 +885,9 @@ -58.97451400756836, -3.382448196411133 ], - "PCA": [ - -30.84, - -18.01 + "UMAP": [ + 9.19, + 3.77 ] }, "genes": { @@ -940,9 +940,9 @@ -58.414215087890625, 9.60135269165039 ], - "PCA": [ - -37.7, - -21.19 + "UMAP": [ + 9.06, + 6.72 ] }, "genes": { @@ -995,9 +995,9 @@ -51.245689392089844, -0.18875575065612793 ], - "PCA": [ - -4.72, - -5.71 + "UMAP": [ + 10.56, + 5.87 ] }, "genes": { @@ -1050,9 +1050,9 @@ -55.345970153808594, 7.696372985839844 ], - "PCA": [ - -18.31, - -10.99 + "UMAP": [ + 9.4, + 6.97 ] }, "genes": { @@ -1105,9 +1105,9 @@ -62.19692611694336, 4.263618469238281 ], - "PCA": [ - -33.27, - -16.0 + "UMAP": [ + 10.14, + 5.79 ] }, "genes": { @@ -1160,9 +1160,9 @@ -60.68256378173828, -2.5718908309936523 ], - "PCA": [ - -33.46, - -12.6 + "UMAP": [ + 9.16, + 3.53 ] }, "genes": { @@ -1215,9 +1215,9 @@ -65.27849578857422, -1.5774139165878296 ], - "PCA": [ - -28.8, - -17.16 + "UMAP": [ + 7.7, + 3.73 ] }, "genes": { @@ -1270,9 +1270,9 @@ -36.909324645996094, 10.284284591674805 ], - "PCA": [ - -37.58, - -11.15 + "UMAP": [ + 9.77, + 4.3 ] }, "genes": { @@ -1325,9 +1325,9 @@ -60.20520782470703, -0.6090396642684937 ], - "PCA": [ - -36.1, - -23.75 + "UMAP": [ + 8.61, + 3.73 ] }, "genes": { @@ -1380,9 +1380,9 @@ -52.42045211791992, 10.036659240722656 ], - "PCA": [ - -37.14, - -7.48 + "UMAP": [ + 9.32, + 6.74 ] }, "genes": { @@ -1435,9 +1435,9 @@ -75.90306854248047, -0.7980395555496216 ], - "PCA": [ - -36.92, - -10.18 + "UMAP": [ + 7.72, + 3.9 ] }, "genes": { @@ -1490,9 +1490,9 @@ -60.503108978271484, 2.4191834926605225 ], - "PCA": [ - -32.96, - -14.78 + "UMAP": [ + 9.31, + 5.64 ] }, "genes": { @@ -1545,9 +1545,9 @@ -60.07191848754883, 8.997148513793945 ], - "PCA": [ - 53.33, - -9.46 + "UMAP": [ + 9.03, + 7.05 ] }, "genes": { @@ -1600,9 +1600,9 @@ -62.83784103393555, 8.216538429260254 ], - "PCA": [ - -34.91, - -14.72 + "UMAP": [ + 15.97, + 6.69 ] }, "genes": { @@ -1655,9 +1655,9 @@ -54.636268615722656, 7.505904674530029 ], - "PCA": [ - -35.51, - -13.59 + "UMAP": [ + 9.45, + 7.26 ] }, "genes": { @@ -1710,9 +1710,9 @@ -35.84719467163086, 11.109227180480957 ], - "PCA": [ - -29.5, - -12.54 + "UMAP": [ + 9.52, + 5.41 ] }, "genes": { @@ -1765,9 +1765,9 @@ -60.25593948364258, -0.6142634153366089 ], - "PCA": [ - -33.82, - -6.36 + "UMAP": [ + 8.49, + 3.74 ] }, "genes": { @@ -1820,9 +1820,9 @@ -68.91304016113281, -2.836963415145874 ], - "PCA": [ - -32.11, - -15.42 + "UMAP": [ + 9.91, + 4.81 ] }, "genes": { @@ -1875,9 +1875,9 @@ -65.57809448242188, 0.16477705538272858 ], - "PCA": [ - 65.16, - 129.06 + "UMAP": [ + 10.14, + 4.71 ] }, "genes": { @@ -1930,9 +1930,9 @@ -55.369773864746094, 14.332426071166992 ], - "PCA": [ - -29.76, - -11.43 + "UMAP": [ + 8.31, + 7.26 ] }, "genes": { @@ -1985,9 +1985,9 @@ -57.06355285644531, -0.5717161893844604 ], - "PCA": [ - -22.61, - -11.86 + "UMAP": [ + 9.83, + 5.32 ] }, "genes": { @@ -2040,9 +2040,9 @@ -59.80588912963867, 5.305624485015869 ], - "PCA": [ - 31.55, - -10.39 + "UMAP": [ + 8.5, + 4.43 ] }, "genes": { @@ -2095,9 +2095,9 @@ -58.81503677368164, 7.680108547210693 ], - "PCA": [ - -30.46, - -13.69 + "UMAP": [ + 9.45, + 6.67 ] }, "genes": { @@ -2150,9 +2150,9 @@ -64.51951599121094, 0.13493074476718903 ], - "PCA": [ - -34.98, - -9.75 + "UMAP": [ + 8.48, + 3.79 ] }, "genes": { @@ -2205,9 +2205,9 @@ -60.99378204345703, 2.5356736183166504 ], - "PCA": [ - -29.26, - -13.47 + "UMAP": [ + 9.74, + 4.8 ] }, "genes": { @@ -2260,9 +2260,9 @@ -69.35445404052734, -3.121276378631592 ], - "PCA": [ - -35.66, - -15.23 + "UMAP": [ + 10.04, + 5.22 ] }, "genes": { @@ -2315,9 +2315,9 @@ -55.75996780395508, 11.697072982788086 ], - "PCA": [ - -6.2, - -8.36 + "UMAP": [ + 8.99, + 7.21 ] }, "genes": { @@ -2370,9 +2370,9 @@ -56.777740478515625, 9.040779113769531 ], - "PCA": [ - -38.78, - -12.26 + "UMAP": [ + 9.23, + 7.08 ] }, "genes": { @@ -2425,9 +2425,9 @@ -64.60640716552734, 5.6718974113464355 ], - "PCA": [ - -25.92, - -18.87 + "UMAP": [ + 10.91, + 5.78 ] }, "genes": { @@ -2480,9 +2480,9 @@ 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