Assertion Failed At 'corelib/line_reader.c:HbnBufferedLineReaderLoadData:86'

[BioYLiu]

Hello,
I installed MECAT2, when I use
mecat.pl correct ecoli_config_file.txt
the ecoli_config_file.txt as follow:

• PROJECT=ecoli
• RAWREADS=/data/third-generation/test/ecoli_filtered.fastq
• GENOME_SIZE=4800000
• THREADS=4
• MIN_READ_LENGTH=2000
• CNS_OVLP_OPTIONS="-kmer_size 13"
• CNS_PCAN_OPTIONS="-p 100000 -k 100"
• CNS_OPTIONS=""
• CNS_OUTPUT_COVERAGE=30
• TRIM_OVLP_OPTIONS="-skip_overhang"
• TRIM_PM4_OPTIONS="-p 100000 -k 100"
• TRIM_LCR_OPTIONS=""
• TRIM_SR_OPTIONS=""
• ASM_OVLP_OPTIONS=""
• FSA_OL_FILTER_OPTIONS="--max_overhang=-1 --min_identity=-1"
• FSA_ASSEMBLE_OPTIONS=""
• CLEANUP=0

the error message as follow:
Smartmatch is experimental at /soft/MECAT2/Linux-amd64/bin/Plgd/Project.pm line 232. 2020-05-06 10:29:15 [Info] Start correcting rawreads. 2020-05-06 10:29:15 [Info] Skip correcting rawreads step 1 mecat2map for outputs are newer. 2020-05-06 10:29:15 [Info] Start partition correction candidates step 2 mecat2pcan. 2020-05-06 10:29:15 [Info] Run script: /data/third-generation/test/ecoli/scripts/cns_pcan.sh 2>&1 |tee /data/third-generation/test/ ecoli/scripts/cns_pcan.sh.log 2020-05-06 10:29:15 [Info] End partition correction candidates step 2 mecat2pcan. 2020-05-06 10:29:15 [Info] Start correcting rawreads step 3 mecat2cns. 2020-05-06 10:29:15 [Info] Run script: /data/third-generation/test/ecoli/scripts/cns_cns.sh 2>&1 |tee /data/third-generation/test/e coli/scripts/cns_cns.sh.log 2020-05-06 10:29:15 [Info] End correcting rawreads step 3 mecat2cns. 2020-05-06 10:29:15 [Info] Start correcting rawreads step 4 make consensus reads list. 2020-05-06 10:29:15 [Info] Run script: /data/third-generation/test/ecoli/scripts/cns_make_list.sh 2>&1 |tee /data/third-generation/ test/ecoli/scripts/cns_make_list.sh.log 2020-05-06 10:29:16 [Info] End correcting rawreads step 4 make consensus reads list. 2020-05-06 10:29:16 [Info] Start correcting rawreads step 5 extract_sequences. 2020-05-06 10:29:16 [Info] Run script: /data/third-generation/test/ecoli/scripts/cns_extract.sh 2>&1 |tee /data/third-generation/te st/ecoli/scripts/cns_extract.sh.log Assertion Failed At 'corelib/line_reader.c:HbnBufferedLineReaderLoadData:86' Expression: 'gzeof(stream)' /data/third-generation/test/ecoli/scripts/cns_extract.sh: line 11: 10416 Aborted /soft/MECAT2/Linux-amd64/bin/mecat2extseqs 4800000 30 /state/ partition1/data/third-generation/test/ecoli/1-consensus/cns_reads_list.txt > /data/third-generation/test/ecoli/1-consensus/cns_final.fasta 2020-05-06 10:29:16 [Error] Failed to run script, 134, /data/third-generation/test/ecoli/scripts/cns_extract.sh