A device for controlling dissolved gas environments in an open-well cell culture. The device is based on the paper DOI: 10.1039/C4LC01168F
- Gas tank (Praxair) with the needed concentration of oxygen
- Pressure regulator Fischer - Scientific
- Push-to-Connect Tube Fitting for Air Straight Adapter, 1/4" Tube OD x 1/4 NPT Female for the pressure regulator to the tubing (Apply teflon tape to these connections-Like a plumber.)
- High pressure tubing O.D..250 or 1/4 or 6.4 mm ID.159 or 4 mm. 95A for the connection of the canister with the flowmwter
- Rotameters we mostly use the 5311G. The maximum flow rate of the lowmeter is 50sccm. A flow of around 30sccm is suggested.
- Push-to-Connect Tube Fitting for Air Straight Adapter for 1/4" Tube OD x 1/8 NPT Male In order to connect the high pressure tube to the rotometers
- Stopcocks with Luer Connections; 4-way; male slip, Non-sterile 3way male valves are very useful
- TYGON LAB TUBING, Non-DEHP, 1/16"ID X 1/8"OD Small tubing to connect the valves with the
- Barbed fitting, Elbow connector,Clear PP, 1/16" - Connectors for the small tubing with the device
- Male luer with lock ring x 5/32" hose barbYou can use these connectors to connect the tygon tube with the device.
- Male luer with lock ring x 1/16" hose barb
UnderConstruction!!