This page demonstrates the major analyses in the published paper (Liu, J., & Zhou, Q. (2025). Evolution of chromosomes and nuclear architectures of amniotes. Nature Communications.). The raw sequencing data and genome assemblies produced in this study were deposited in NCBI BioProject PRJNA1219809. if any questions, please contract Jing Liu: liujing1993@zju.edu.cn or Qi Zhou: zhouqi1982@zju.edu.cn