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ADPKD-ECS

Public-data program on the endocannabinoid system and cannabis-related questions in autosomal dominant polycystic kidney disease. No wet lab.

This git tree is the program. Each confirmatory question is its own seal.

Study Question (one line) Status
1 — Atlas kill (this directory’s root SEAL files) Frozen CNR1 in ADPKD PT (GSE185948) + DKD specificity (GSE195460) GIT-SEALED; OSF; Decide 2026-09-01: C fail
2 — Safety map studies/cannabis-safety/ — plant vs CBD vs synthetics vs CB1 drugs; Jynarque / Epidiolex GIT-SEALED; OSF; v1 map complete (Decide 2026-08-31)
3 — Chemistry Docking / ADMET Parked in EXPLORE.md. Study 1 Decide (C fail) does not license docking.
Read first What it is
QUESTION.md Study 1 one sentence
KILL.md Named confounds + pass/fail (study 1)
CLAIMS.md What study 1 may / may not say
PROTOCOL.md Study 1 confirmatory fields (git-sealed; OSF)
STATUS.md You are here (study 1)
docs/briefing/cannabinoid-adpkd-pathways.md Source briefing (not a protocol)
docs/SUPERPOWERS.md How Science Superpowers maps onto SEAL
EXPLORE.md Parked work including study 3

Protocol seal (study 1): GIT-SEALED 2026-08-30 (db44b30). OSF: https://osf.io/7g3tn/ . Decide: Gate C fail (2026-09-01). Stop this ADPKD accession; do not dock.

Study text is CC BY 4.0. Third-party datasets stay under their original licenses.

Setup

Python 3.12 and uv:

uv sync

The scrna extra (scanpy) is for after study 1 Seal. Unit 00 is the first runner.

Process kernel: .seal/E2E_FLOW.md. This folder is study 1: E2E_FLOW.md.

Chat shorthand

Explore → Question → Kill → Protocol → Clarify → Analyze → Seal (OSF) → Unit → Run → Converge → Decide → Archive.

Science Superpowers skills fire inside those stages (docs/SUPERPOWERS.md). After Seal, say Unit then Run. Do not say Run on outcomes before Seal. Do not use study 1 CNR1 results to write study 2 claims, or the reverse.

About

Public-data computational study of the endocannabinoid system in ADPKD. v1 is a pre-registered kill test of CNR1 localization in GSE185948. Not a harvest lab. Not clinical advice.

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