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Dryas_GenomeBC

Differential DNA methylation and RNAseq analyses

1_RNAseq - mRNA

  • RSEM for mapping to transcriptome
  • EdgeR for transcript count normalization, differential expression analyses and plotting heat maps

2_Methylseq

  • nf-core Methylseq for WGBS mapping and calling methylated C's
  • Run on wild, phenology, and seedling data

3_DMRs

  • Metilene and Methylkit for detecting DMRs
  • Run for detecting various Wild, Seedling, and Phenology DMRS
  • Scripts for preparing files for Metilene and running metilene

4_Bedgraphs_Intersections

  • Bedtools for comparisons
  • Crosses over DMRs, to find intersecting or non-intersecting DMRS

5_Blast

  • Script for extending DMRS, and mapping to fasta file
  • Notes on Blasting DMRS to reference and to NCBI

6_Interproscan

  • Scripts for running interproscan on a set of files
  • Script on running interproscan on divided subseq files
  • Script for formatting file to run on interproscan
  • Notes on running interproscan on both Methylation and RNA data, and on comparing/combining the two

7_Gene_ontology

  • ErmineJ for GO term enrichment

8_Plotting_Comparisons

  • Several scripts for plotting
  • General notes on heatmap plotting (RNA and methylation)
  • Notes on inheritence plotting

9_SNP_calling

  • BS-SNP for SNP calling from bisulphite treated DNA
  • Basic popgen analyses

10_Phenotypes

  • Comparing phenotypes and traits of the warmed and control plants

Old_Files_Semi_Sorted - Temporary

  • Notes on original runs of certain anlyses + analyses not yet used/completed

Unused scripts

  • Phenogram script for interproscan + annotation file to format to phenogram
  • gff3 to cds conversion

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DNA methylation and RNAseq analyses

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