EBV lytic viewer: cascade heatmap, CAGE class map, and RNA–protein context

[eulestadt]

Interactive viewer for Yuan 2006 Table 1 (59 complete oligo series) with additive Focus-panel views of published lytic-cycle relationships. Live: https://lytic.phoenix.boston

What stayed the same

The main chart is unchanged: real-hour x-axis, measured points only, ghosts of all 59 genes, 10–90% band + median, pin/hover, log2 vs linear fold, MultiCAST calibrators as the default pins. CAGE filters still affect the gene list only, not the ghosts.

What was added (Focus panel)

Published recommendations, not dual-axis overlays on the Yuan chart:

• Pinned RNA cascade heatmap — log2 fold vs 0 h, genes ordered by peak hour (Yuan-style cascade; Weekes/Ersing temporal heatmaps).
• CAGE kinetic map — BALF2 ratio vs BDLF4 ratio with 0.1 / 0.5 cutoffs (Djavadian et al. PLOS Pathog. 2018, Fig. 4). Not a time series.
• 48 h RNA vs Akata protein scatter — same cell/trigger as Yuan, not paired aliquots (Ersing et al. Cell Rep. 2017; Weekes et al. Cell 2014 CMV). Color = CAGE class, identity line, Pearson r on the plotted log2 axes.
• RNA and protein as separate tracks — independent y-scales, shared hour axis (avoid dual-axis magnitude comparison).

Presets: Calibrators, Top 8 peaks, Cascade examples, Array ≠ CAGE (BFRF1, BMRF2, BKRF4, BVRF1).

Sources

• Yuan et al. J Virol. 2006;80:2548–2565 (Table 1)
• Djavadian, Hayes, Johannsen. PLOS Pathog. 2018;14:e1007114
• Ersing, Nobre, Gewurz, Weekes et al. Cell Rep. 2017;19:1479–1493