Average Nucleotide Identity

[lskatz]

Hi, I am wondering if you could add ANI per species in QC? It would be a range of within species ANI values and also the next closest species and what the inter-species ANI value is. It looks like you used Sylph and so I hope it is easy to extract that. I am hoping that we might be able to gleam a similar graph to our Lindsey 2023 paper for ANI but for all the bacteria.

https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1225207/full

[happykhan]

Hi Lee,

Sylph does have ANI but this is ANI to the reference genome it finds in its database and I think there are multiple reference genomes per species. So i am not sure how we can normalise it to give this kind of distribution?

[lskatz]

Maybe in a future version of this if the data are updated? But I agree that if everything is measured against a reference then you would not be able to produce this distribution.