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first install the Metamorph nd & ROI files importer to ImageJ if you haven't already.
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Run the stack builder macro (stack-builder.ijm) selecting the calibration and expiriment nd files. ( You may have to change *.TIF to *tif on some Unix systems)
$ for old in *.TIF; do mv $old
basename $old .TIF.tif; done -
Choose the main folder with all your images and .nd files as the 'Experiment Directory'. The macro will then rename this folder as RAW-MDA and make a folder around it with the original foldername.
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Choose the nd files corresponding to your calibration points and finally your experimental timecourse.
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The macro will make stacks of all the positions and organize them into folders. Then it will measure the intensities in one huge table.
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Save the table and use makecolumns.m to reshape the data into positions.
Folders and files
| Name | Name | Last commit date | ||
|---|---|---|---|---|