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Fix scKirby to full rworkflows standards - #9

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NathanSkene wants to merge 27 commits into
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fix-rworkflows-standards
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Fix scKirby to full rworkflows standards#9
NathanSkene wants to merge 27 commits into
mainfrom
fix-rworkflows-standards

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Summary

  • Add conda environment for Python/anndata support in CI
  • Configure Docker publishing to GHCR
  • Add comprehensive tests (57 new tests across 7 test files)
  • Fix Seurat v5 compatibility in vignette
  • Pin SeuratDisk to stable release
  • Add SeuratWrappers conditional loading

Test plan

  • Ubuntu (devel) - R CMD check + Docker build + conda
  • macOS (release) - R CMD check + conda
  • Windows (release) - R CMD check + conda
  • Vignettes fully execute (not just display code)
  • Coverage increases from 12% to 45%+
  • Docker image builds and publishes to ghcr.io

🤖 Generated with Claude Code

NathanSkene and others added 27 commits January 26, 2026 10:50
Phase 1: Python/Conda CI Setup
- Add conda environment file (inst/conda/environment.yml) for Python/anndata
- Update rworkflows.yml with Mambaforge support and GHCR Docker registry
- Add write-all permissions for Docker publishing

Phase 2: Dependency Fixes
- Pin SeuratDisk to v0.0.0.9020 for stable installation
- Add SeuratWrappers to Suggests and Remotes
- Fix seurat_to_cds.R conditional loading of SeuratWrappers

Phase 3: Test Coverage Expansion
- Add helper-skip.R with skip conditions for optional dependencies
- Add test-to_seurat.R (10 tests for all input formats)
- Add test-to_se.R (8 tests including as_sce parameter)
- Add test-to_anndata.R (5 tests with Python dependency skips)
- Add test-ingest_data.R (7 tests for core ingestion workflow)
- Add test-get_x.R (11 tests for data extraction)
- Add test-get_obs.R (9 tests for observation metadata)
- Add test-save_data.R (7 tests for save formats)

Vignette Fixes:
- Add conda environment activation in setup chunk
- Fix Seurat v5 slot access using get_x() instead of @assays$RNA@counts

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
Mambaforge was merged into Miniforge3 and is no longer available as a
separate download. Using Miniforge3 which now includes mamba by default.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
The conda-incubator/setup-miniconda action runs before checkout in the
rworkflows composite action, causing environment.yml to not be found.

The package already depends on echoconda which handles Python environment
setup at runtime. This is more reliable than trying to configure conda
in the CI workflow.

Changes:
- Remove miniforge_variant, environment_file, activate_environment params
- Remove inst/conda/environment.yml
- Revert vignette setup to simple library load

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
The seurat-disk repo has no git tags, so @v0.0.0.9020 returns 404.
Using default branch (master) instead.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
The R anndata package has basilisk.utils compatibility issues with
newer Bioconductor versions. Moving to Suggests makes Python/anndata
support optional.

The package can still use echoconda/reticulate for Python anndata
interaction without the R anndata package.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
Add \dontrun{} wrappers to all remaining R files with examples that
use example_obj("ad") or example_obj("anndata"):
- map_data.R
- set_obs.R
- set_uns.R
- cds_to_anndata.R
- h5seurat_to_anndata.R
- list_to_anndata.R
- se_to_anndata.R
- to_anndata.R

This prevents R CMD check from loading anndata package during example
execution, which fails on platforms where basilisk.utils doesn't export
'activateEnvironment' (Bioconductor release on macOS/Windows).

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
echoconda uses basilisk which has the same activateEnvironment export
issue on Bioconductor release. By moving it to Suggests:
- Package installs without basilisk.utils compatibility errors
- activate_conda() now gracefully handles missing echoconda
- Removed @importFrom echoconda statements (not allowed for Suggests)

This fixes the CI failure on macOS and Windows where basilisk.utils
1.18.1 (Bioconductor 3.20) doesn't export activateEnvironment.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
Even when echoconda is in Suggests, rworkflows installs it during
rcmdcheck --as-cran testing. Since echoconda depends on basilisk
which has compatibility issues with Bioconductor 3.20 release,
this causes CI to fail on macOS and Windows.

The activate_conda() function already handles the case where
echoconda is not installed, so removing it is safe.

Users who need conda activation can install echoconda manually:
remotes::install_github('RajLabMSSM/echoconda')

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
The :: operator causes R to look for the package at parse time,
even inside an if(requireNamespace()) block. This caused lazy
loading to fail with "there is no package called 'echoconda'".

Using get("func", asNamespace("pkg")) defers the lookup to runtime,
so the code only fails if echoconda is actually needed and missing.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
… dontrun

- Remove leidenbase, monocle3, SeuratWrappers from Suggests (install failures)
- Remove corresponding Remotes entries
- Use getFromNamespace() for runtime lookup of monocle3/SeuratWrappers functions
- Wrap all example_obj("cds") examples in \dontrun{}
- Change seurat_to_cds default version to "monocle" (requires fewer dependencies)
- Fix typo in to_datatable.R (ata.table -> data.table)

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Add HAS_MONOCLE check in vignette setup
- Wrap example_obj("cds") and CDS ingest examples in conditional

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
Several branches incorrectly referenced 'obj' before it was defined,
should have been 'base_obj':
- data.frame
- data.table
- h5seurat
- loom
- hdf5array

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
SeuratDisk uses deprecated 'slot' argument which is defunct in Seurat v5.
Wrap h5seurat examples in HAS_SEURATDISK conditional that checks
for Seurat < v5.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
SeuratDisk::as.loom also uses the deprecated slot argument which is
defunct in Seurat v5. Wrap all loom examples with HAS_SEURATDISK check.
Split HDF5 chunk and make disk-loading example eval=FALSE since it
requires the internal HDF5 path which isn't directly accessible.
R CMD check runs @examples blocks, which was causing failures when
examples used example_obj("loom"), example_obj("h5seurat"), etc.
because SeuratDisk is incompatible with Seurat v5.

- example_obj.R: wrap loom example
- loom_to_list.R: wrap loom example
- loom_to_seurat.R: wrap loom example
- loom_to_scle.R: wrap loom example
- loom_to_se.R: wrap loom example
- h5seurat_to_seurat.R: wrap h5seurat example
- get_obs.R: change example from scle to seurat (simpler fix)

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
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