A web-based Python application for predicting the effective translation rate (ETR) from a provided full RBS sequence and generating the RBS core sequence based on a desired ETR value.

The application requires Python 3.12 and the modules listed in the standard requirements.txt file. To launch the web-based GUI, simply run the app.py script.

The source data were obtained from: Bonde, M. T. et al. Predictable tuning of protein expression in bacteria. Nat Methods 13, 233–236 (2016).

Author: Pavel Zach (pazac@upv.edu.es)