simoncchu/SpliceJumper
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Usage: ./SpliceJumper [options]
Required options:
-P/G indicates collecting features / indicates output results
-r FILE reference file(indexed)
-b/i FILE input bam/prediction file(index and sorted)
-o FILE output file name
-l INT read length
Optimal options
-c INT slack value for split position with default 3
-m DOUBLE mean insert size
-v DOUBLE standard variation of insert size
1.Feature collection:
./SpliceJumper -P -r ./human_g1k_v37.fasta -b ./simulated_data_input.bam -o test_sim.txt -l 100 -c 5
2. Training and prediction
2.1 Training
python easy.py training_data
2.2 Predicting
..\windows\svm-predict data.scale Trained.model predictResult
3. Output generated splicing junctions
./SpliceJumper -G -i prediction.txt -o result.txt