brainstorm-cytometry created by GitHub Classroom
- mkuijs: Merel Kuijs
- lbourguignon: Lucie Bourguignon
- parizkh: Hana Parizkova
- retogerber: Reto Gerber
- RBetschart : Raphael Betschart
- cell type and cell count discovery; enables cell sorting
- cells labeled with fluorescent antibodies are brought into a single cell suspension, which is illuminated with a laser light beam: cellular features produce light scatter (forward angle scatter and side angle scatter)
- different cell types produce forward scatter and side scatter to a different extent
- output: two-parameter plots and single-parameter histograms
- combination of plasma mass spectrometry and time-of-flight mass spectrometry
- cellular proteins labeled with isotope-conjugated antibodies, metal signals analyzed by time-of-flight mass spectrometer
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FC is cheaper and faster, but needs compensation for fluorescence overlap.
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MC is able to detect more parameters per cell than FC, but requires more sample preparation.