Skip to content
Merged
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
211 changes: 211 additions & 0 deletions docs/notebooks/spatialdata_image_3d.ipynb
Original file line number Diff line number Diff line change
@@ -0,0 +1,211 @@
{
"cells": [
{
"cell_type": "code",
"execution_count": 12,
"id": "1ca97045-ab06-4700-9870-5eef11f1e4e1",
"metadata": {},
"outputs": [],
"source": [
"import easy_vitessce as ev"
]
},
{
"cell_type": "code",
"execution_count": 13,
"id": "39f4b2fd-d3fd-4d27-83e2-9b804bd40863",
"metadata": {},
"outputs": [],
"source": [
"import zarr\n",
"\n",
"zarr_group = zarr.open(\n",
" \"s3://idr/zarr/v0.4/idr0062A/6001240.zarr\",\n",
" storage_options={\n",
" \"anon\": True,\n",
" \"client_kwargs\": {\n",
" \"endpoint_url\": \"https://uk1s3.embassy.ebi.ac.uk\"\n",
" }\n",
" }\n",
")"
]
},
{
"cell_type": "code",
"execution_count": 14,
"id": "060f1e78-903f-4943-8d16-56b1e74169b1",
"metadata": {},
"outputs": [
{
"data": {
"text/plain": [
"['0', '1', '2', 'labels']"
]
},
"execution_count": 14,
"metadata": {},
"output_type": "execute_result"
}
],
"source": [
"list(zarr_group.keys())"
]
},
{
"cell_type": "code",
"execution_count": 15,
"id": "eb38c0d8-f0a1-4a03-8055-378a1895cefb",
"metadata": {},
"outputs": [
{
"data": {
"text/plain": [
"{'_creator': {'name': 'omero-zarr', 'version': '0.4.0'},\n",
" 'multiscales': [{'axes': [{'name': 'c', 'type': 'channel'},\n",
" {'name': 'z', 'type': 'space', 'unit': 'micrometer'},\n",
" {'name': 'y', 'type': 'space', 'unit': 'micrometer'},\n",
" {'name': 'x', 'type': 'space', 'unit': 'micrometer'}],\n",
" 'datasets': [{'coordinateTransformations': [{'scale': [1.0,\n",
" 0.5002025531914894,\n",
" 0.3603981534640209,\n",
" 0.3603981534640209],\n",
" 'type': 'scale'}],\n",
" 'path': '0'},\n",
" {'coordinateTransformations': [{'scale': [1.0,\n",
" 0.5002025531914894,\n",
" 0.7207963069280418,\n",
" 0.7207963069280418],\n",
" 'type': 'scale'}],\n",
" 'path': '1'},\n",
" {'coordinateTransformations': [{'scale': [1.0,\n",
" 0.5002025531914894,\n",
" 1.4415926138560835,\n",
" 1.4415926138560835],\n",
" 'type': 'scale'}],\n",
" 'path': '2'}],\n",
" 'version': '0.4'}],\n",
" 'omero': {'channels': [{'active': True,\n",
" 'coefficient': 1.0,\n",
" 'color': '0000FF',\n",
" 'family': 'linear',\n",
" 'inverted': False,\n",
" 'label': 'LaminB1',\n",
" 'window': {'end': 1500.0, 'max': 65535.0, 'min': 0.0, 'start': 0.0}},\n",
" {'active': True,\n",
" 'coefficient': 1.0,\n",
" 'color': 'FFFF00',\n",
" 'family': 'linear',\n",
" 'inverted': False,\n",
" 'label': 'Dapi',\n",
" 'window': {'end': 1500.0, 'max': 65535.0, 'min': 0.0, 'start': 0.0}}],\n",
" 'id': 1,\n",
" 'rdefs': {'defaultT': 0, 'defaultZ': 118, 'model': 'color'},\n",
" 'version': '0.4'}}"
]
},
"execution_count": 15,
"metadata": {},
"output_type": "execute_result"
}
],
"source": [
"dict(zarr_group.attrs)"
]
},
{
"cell_type": "code",
"execution_count": 16,
"id": "12f5ba16-4adc-423b-be4e-629d41c256cd",
"metadata": {},
"outputs": [],
"source": [
"img_arr = zarr_group['/1']"
]
},
{
"cell_type": "code",
"execution_count": 17,
"id": "a51fcb74-94b0-4a43-84af-89ee6fddae15",
"metadata": {},
"outputs": [],
"source": [
"from spatialdata import SpatialData\n",
"from spatialdata.models import Image3DModel\n",
"\n",
"image = Image3DModel.parse(img_arr[()], dims=(\"c\",\"z\",\"y\",\"x\"), c_coords=[\"LaminB1\", \"Dapi\"])"
]
},
{
"cell_type": "code",
"execution_count": 18,
"id": "d2b2fc74-87ba-4c84-a577-b913726cb5a7",
"metadata": {},
"outputs": [
{
"data": {
"text/plain": [
"SpatialData object\n",
"└── Images\n",
" └── 'image': DataArray[czyx] (2, 236, 137, 135)\n",
"with coordinate systems:\n",
" ▸ 'global', with elements:\n",
" image (Images)"
]
},
"execution_count": 18,
"metadata": {},
"output_type": "execute_result"
}
],
"source": [
"sdata = SpatialData(images={\"image\": image})\n",
"sdata"
]
},
{
"cell_type": "code",
"execution_count": 20,
"id": "abca9477-feb7-4317-b77d-af021d5d30f0",
"metadata": {},
"outputs": [],
"source": [
"vw = (\n",
" sdata\n",
" .pl.render_images(\"image\")\n",
" .pl.show()\n",
")"
]
},
{
"cell_type": "code",
"execution_count": null,
"id": "6e09f547-e1d3-4191-a5ae-6cce66e40faa",
"metadata": {},
"outputs": [],
"source": [
"vw.config.web_app()"
]
}
],
"metadata": {
"kernelspec": {
"display_name": "Python 3 (ipykernel)",
"language": "python",
"name": "python3"
},
"language_info": {
"codemirror_mode": {
"name": "ipython",
"version": 3
},
"file_extension": ".py",
"mimetype": "text/x-python",
"name": "python",
"nbconvert_exporter": "python",
"pygments_lexer": "ipython3",
"version": "3.12.5"
}
},
"nbformat": 4,
"nbformat_minor": 5
}